Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 30, Issue 9, 2011, Pages 1127-1134

  Josefson, P ^a   Stralin K ^a   Ohlin, A ^a   Ennefors, T ^a   Dragsten, B ^a   Andersson, L ^a   Fredlund, H ^a   Molling P ^a   Olcen P ^a  

^a ÖREBRO UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; AGED; ARTICLE; ASPERGILLUS; BLOOD CULTURE; BLOODSTREAM INFECTION; CANDIDA; COMPARATIVE EFFECTIVENESS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; DIFFERENTIAL DIAGNOSIS; ESCHERICHIA COLI; FEMALE; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HUMAN; KLEBSIELLA; MAJOR CLINICAL STUDY; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PREDICTIVE VALUE; PRIORITY JOURNAL; PROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE;

EID: 80054723483     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-011-1201-6     Document Type: Article

References (23)

1. Kumar A, Roberts D, Wood KE, Light B, Parrillo JE, Sharma S, Suppes R, Feinstein D, Zanotti S, Taiberg L, Gurka D, Kumar A, Cheang M (2006) Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34(6):1589-1596 (Pubitemid 43810035)
2. Beekmann SE, Diekema DJ, Chapin KC, Doern GV (2003) Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J Clin Microbiol 41(7):3119-3125 (Pubitemid 36842309)
3. Kollef MH, Sherman G, Ward S, Fraser VJ (1999) Inadequate antimicrobial treatment of infections: A risk factor for hospital mortality among critically ill patients. Chest 115(2):462-474 (Pubitemid 29083057)
4. Ibrahim EH, Sherman G, Ward S, Fraser VJ, Kollef MH (2000) The influence of inadequate antimicrobial treatment of bloodstream infections on patient outcomes in the ICU setting. Chest 118(1):146-155 (Pubitemid 30485502)
5. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM (2004) New developments in the diagnosis of bloodstream infections. Lancet Infect Dis 4(12):751-7606.
6. Schrenzel J (2007) Clinical relevance of new diagnostic methods for bloodstream infections. Int J Antimicrob Agents 30(Suppl 1): S2-S6
7. Mancini N, Carletti S, Ghidoli N, Cichero P, Burioni R, Clementi M (2010) The era of molecular and other non-culture-based methods in diagnosis of sepsis. Clin Microbiol Rev 23(1):235-251
8. Meier A, Persing DH, Finken M, Böttger EC (1993) Elimination of contaminating DNA within polymerase chain reaction reagents: Implications for a general approach to detection of uncultured pathogens. J Clin Microbiol 31(3):646-652 (Pubitemid 23059882)
9. Lehmann LE, Hunfeld KP, Emrich T, Haberhausen G, Wissing H, Hoeft A, Stüber F (2008) A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol 197(3):313-324
10. Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH (2003) Manual of clinical microbiology, 8th edn. ASM Press, Washington, DC
11. Lau A, Chen S, Sorrell T, Carter D, Malik R, Martin P, Halliday C (2007) Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. J Clin Microbiol 45(2):380-385
12. Abdeldaim G, Herrmann B, Mölling P, Holmberg H, Blomberg J, Olcén P, Strålin K (2010) Usefulness of real-time PCR for lytA, ply, and Spn9802 on plasma samples for the diagnosis of pneumococcal pneumonia. Clin Microbiol Infect 16(8):1135-1141
13. Hall KK, Lyman JA (2006) Updated review of blood culture contamination. Clin Microbiol Rev 19(4):788-802 (Pubitemid 44582981)
14. Kassis C, Rangaraj G, Jiang Y, Hachem RY, Raad I (2009) Differentiating culture samples representing coagulase-negative staphylococcal bacteremia from those representing contamination by use of time-to-positivity and quantitative blood culture methods. J Clin Microbiol 47(10):3255-3260
15. Mancini N, Clerici D, Diotti R, Perotti M, Ghidoli N, De Marco D, Pizzorno B, Emrich T, Burioni R, Ciceri F, Clementi M (2008) Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies. J Med Microbiol 57(Pt 5):601-604 (Pubitemid 351632907)
16. von Lilienfeld-Toal M, Lehmann LE, Raadts AD, Hahn-Ast C, Orlopp KS, Marklein G, Purr I, Cook G, Hoeft A, Glasmacher A, Stüber F (2009) Utility of a commercially available multiplex realtime PCR assay to detect bacterial and fungal pathogens in febrile neutropenia. J Clin Microbiol 47(8):2405-2410
17. Varani S, Stanzani M, Paolucci M, Melchionda F, Castellani G, Nardi L, Landini MP, Baccarani M, Pession A, Sambri V (2009) Diagnosis of bloodstream infections in immunocompromised patients by real-time PCR. J Infect 58(5):346-351
18. Casalta JP, Gouriet F, Roux V, Thuny F, Habib G, Raoult D (2009) Evaluation of the LightCycler SeptiFast test in the rapid etiologic diagnostic of infectious endocarditis. Eur J Clin Microbiol Infect Dis 28:569-573
19. Lamoth F, Jaton K, Prod'hom G, Senn L, Bille J, Calandra T, Marchetti O (2010) Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia. J Clin Microbiol 48(10):3510-3516
20. Louie RF, Tang Z, Albertson TE, Cohen S, Tran NK, Kost GJ (2008) Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia. Crit Care Med 36(5):1487-1492 (Pubitemid 351620647)
21. Tsalik EL, Jones D, Nicholson B, Waring L, Liesenfeld O, Park LP, Glickman SW, Caram LB, Langley RJ, van Velkinburgh JC, Cairns CB, Rivers EP, Otero RM, Kingsmore SF, Lalani T, Fowler VG, Woods CW (2010) Multiplex PCR to diagnose bloodstream infections in patients admitted from the emergency department with sepsis. J Clin Microbiol 48(1):26-33
22. Avolio M, Diamante P, Zamparo S, Modolo ML, Grosso S, Zigante P, Tosoni N, De Rosa R, Stano P, Camporese A (2010) Molecular identification of bloodstream pathogens in patients presenting to the emergency department with suspected sepsis. Shock 34(1):27-30
23. Westh H, Lisby G, Breysse F, Böddinghaus B, Chomarat M, Gant V, Goglio A, Raglio A, Schuster H, Stüber F, Wissing H, Hoeft A (2009) Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect 15(6):544-551