PCR identification of bacteria in blood culture does not fit the daily workflow of a routine microbiology laboratory

Journal of Clinical Microbiology

Volumn 50, Issue 3, 2012, Pages 1031-1033

  Karumaa, Santra ^a   Kärpänoja, Pauliina ^a   Sarkkinen, Hannu ^a  

^a Clinical Microbiology   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; VANCOMYCIN;

ACINETOBACTER BAUMANNII; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BLOOD CULTURE; CITROBACTER FREUNDII; CITROBACTER KOSERI; DNA ISOLATION; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENTEROBACTER INTERMEDIUM; ENTEROCOCCUS CASSELIFLAVUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS GALLINARUM; ESCHERICHIA COLI; GENOTYPE; GRAM NEGATIVE BACTERIUM; HAEMOPHILUS INFLUENZAE; HAEMOPHILUS PARAINFLUENZAE; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; MECA GENE; MICROBIOLOGY; NONHUMAN; PANTOEA AGGLOMERANS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEUS MIRABILIS; PSEUDOMONAS AERUGINOSA; SALMONELLA ENTERICA; SERRATIA MARCESCENS; SHIGELLA; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS HAEMOLYTICUS; STAPHYLOCOCCUS HOMINIS; STAPHYLOCOCCUS SIMULANS; STAPHYLOCOCCUS WARNERI; STENOTROPHOMONAS MALTOPHILIA; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS ANGINOSUS; STREPTOCOCCUS BOVIS; STREPTOCOCCUS CONSTELLATUS; STREPTOCOCCUS DYSGALACTIAE; STREPTOCOCCUS INTERMEDIUS; STREPTOCOCCUS MITIS; STREPTOCOCCUS MUTANS; STREPTOCOCCUS ORALIS; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS PYOGENES; STREPTOCOCCUS SANGUINIS; VANA GENE; VANB GENE; VANC1 GENE; VANC2 GENE; VANC3 GENE; WORKFLOW;

BACTEREMIA; BACTERIA; BACTERIOLOGICAL TECHNIQUES; BLOOD; DNA PRIMERS; GERMANY; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WORKFLOW;

EID: 84857410354     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01271-11     Document Type: Article

References (14)

1. Denys GA, Carey RB. 1992. Identification of Streptococcus pneumoniae with a DNA probe. J. Clin. Microbiol. 30:2725-2727.
2. Eigner U, Weizenegger M, Fahr A-M, Witte W. 2005. Evaluation of rapid direct assay for identification of bacteria and the mecA and van genes from positive-testing blood cultures. J. Clin. Microbiol. 43:5256-5262. (Pubitemid 41484034)
3. Hassan-King M, et al. 1996. Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay. J. Clin. Microbiol. 34:2030-2032. (Pubitemid 26239249)
4. Jolkkonen S, Kärpänoja P, Sarkkinen H. 2009. Direct identification of Gram-positive cocci and Gram-negative rods from bio-Merieux blood culture bottles by Geno-Type blood culture assay (HAIN), abstr. P1829. Eur. Congr. Clin. Microbiol. Infect. Dis., Helsinki, Finland., 16-19 May 2009.
5. Kempf VAJ, Trebesius K, Autenrieth IB. 2000. Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J. Clin. Microbiol. 38:830-838. (Pubitemid 30082689)
6. Lin S, Yan S. 2010. Molecular methods for pathogen detection in blood. Lancet 375:178-179.
7. Lindholm L, Sarkkinen H. 2004. Direct identification of gram-positive cocci from routine blood cultures by using AccuProbe tests. J. Clin. Microbiol. 42:5609-5613. (Pubitemid 39664344)
8. Mancini N, et al. 2008. Molecular diagnosis of sepsis in neutropenic patiens with haematological malignancies. J. Med. Microbiol. 57:601-604. (Pubitemid 351632907)
9. Oliveira K, Procop GW, Wilson D, Coull J, Stender H. 2002. Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescent in situ hybridization with peptide nucleic acid probes. J. Clin. Microbiol. 40:247-251. (Pubitemid 34027631)
10. Oliveira K, et al. 2003. Direct identification of Staphylococcus aureus from positive blood culture bottles. J. Clin. Microbiol. 41:889-891. (Pubitemid 36184223)
11. Peters RPH, et al. 2006. Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice. J. Clin. Microbiol. 44:119-123. (Pubitemid 43100589)
12. Prère M-F, Baron O, Fayet O. 2007. Rapid identification of bacteria, mecA and van genes from blood cultures. Pathol. Biol. (Paris) 55:375-377. (Pubitemid 47633097)
13. Rudolph KM, Parkinson Black AJCM, Mayer LW. 1993. Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia. J. Clin. Microbiol. 31:2661-2666. (Pubitemid 23284898)
14. Tissari P, et al. 2010. Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study. Lancet 375:224-230.