Biodegradable Bioplastics from Fermented Sludge, Wastes, and Effluents

Biological Sludge Minimization and Biomaterials/Bioenergy Recovery Technologies

Volumn , Issue , 2012, Pages 465-498

  Paul, Etienne ^a,b,c,d   Neuhauser, Elisabeth ^a,b,c,d   Liu, Yu ^e  

^a UNIVERSITÉ DE TOULOUSE   (France)

^b INSA   (France)

^c UMR792 INGÉNIERIE DES SYSTÈMES BIOLOGIQUES ET DES PROCÉDÉS   (France)

^d CNRS   (France)

^e NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

Biodegradable bioplastics from fermented sludge, waste and effluent; Biological transformations by MC fermentation, for chemical or energy; Cupriavidus necator, microorganisms in PHA energy accumulation; Effluents, and sludge organic matter conversion to PHA; PHA synthesis, metabolism and ADF, PHA store for energy balance

Indexed keywords

EID: 84885357894     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781118309643.ch15     Document Type: Chapter

References (111)

1. Albuquerque M. G. E., Eiroa M., Torres C., Nunes B. R., Reis M. A. M. (2007) Strategies for the development of a side stream process for polyhydroxyalkanoate (PHA) production from sugar cane molasses. Journal of Biotechnology 130(4), 411-421.
2. Albuquerque M. G. E., Torres C., Bengtsson S., Werker A., Reis M. A. M. (2008) Strategies for culture selection in a three-stage PHA production process from sugar cane molasses. In: Proceedings II of the 4th IWA Specialised Conference on Sequencing Batch Reactor Technology (SBR4) Rome, April 7-10, pp.; 2-4.
3. Albuquerque M. G. E., Bengtsson S., Martino V., Pollet E., Reis M. A. M. (2010a) Ecoengineering of mixed microbial cultures to develop a cost-effective bioplastic (PHA) production process from a surplus feedstock-sugar molasses. Journal of Biotechnology 150, S70-S70.
4. Albuquerque M. G. E., Concas S., Bengtsson S., Reis M. A. M. (2010b). Mixed culture polyhydroxyalkanoates production from sugar molasses: the use of a 2-stage CSTR system for culture selection. Bioresource Technology 101(18), 7123-7133.
5. Anderson A. J., Dawes E. A. (1990) Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiological Reviews 54(4), 450-472.
6. Anderson A. J., Haywood G. W., Dawes E. A. (1990) Biosynthesis and composition of bacterial poly(hydroxyalkanoates). International Journal of Biological Macromolecules 12(2), 102-105.
7. Anderson A. J., Williams D. R., Taidi B., Dawes E. A., Ewing D. F. (1992) Studies on copolyester synthesis by Rhodococcus-ruber and factors influencing the molecular mass of polyhydroxybutyrate accumulated by Methylobacterium-extorquens and Alcaligeneseutrophus. FEMS Microbiology Reviews 103(2-4), 93-101.
8. Aragao G. M. F., Lindley N. D., Uribelarrea J. L., Pareilleux A. (1996). Maintaining a controlled residual growth capacity increases the production of polyhydroxyalkanoate copolymers by Alcaligenes eutrophus. Biotechnology Letters 18(8), 937-942.
9. Beccari M., Dionisi D., Giuliani A., Majone M., Ramadori R. (2002) Effect of different carbon sources on aerobic storage by activated sludge. Water Science and Technology 45(6), 157-168.
10. Beccari M., Bertin L., Dionisi D., Fava F., Lampis S., Majone M., Valentino F., Vallini G., Villano M. (2009) Exploiting olive oil mill effluents as a renewable resource for production of biodegradable polymers through a combined anaerobic-aerobic process. Journal of Chemical Technology and Biotechnology 84(6), 901-908.
11. Bengtsson S. (2009) The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates. Biotechnology and Bioengineering 104(4), 698-708.
12. Bengtsson S., Werker A., Christensson M., Welander T. (2008a) Production of polyhydroxyalkanoates by activated sludge treating a paper mill wastewater. Bioresource Technology 99(3), 509-516.
13. Bengtsson S., Werker A., Welander T. (2008b) Production of polyhydroxyalkanoates by glycogen accumulating organisms treating a paper mill wastewater. Water Science and Technology 58(2), 323-330.
14. Beun J. J., Paletta F., van Loosdrecht M. C. M., Heijnen J. J. (2000a) Stoichiometry and kinetics of poly-beta-hydroxybutyrate metabolism in aerobic, slow growing, activated sludge cultures. Biotechnology and Bioengineering 67(4), 379-389.
15. Beun J. J., Verhoef E. V., van Loosdrecht M. C. M., Heijnen J. J. (2000b) Stoichiometry and kinetics of poly-beta-hydroxybutyrate metabolism under denitrifying conditions in activated sludge cultures. Biotechnology and Bioengineering 68(5), 496-507.
16. Beun J. J., Dircks K., Van LoosdrechtM. C.M., Heijnen J. J. (2002) Poly-beta-hydroxybutyrate metabolism in dynamically fed mixed microbial cultures. Water Research 36(5), 1167-1180.
17. Braunegg G., Bona R., Koller M. (2004) Sustainable polymer production. Polymer Plastics Technology and Engineering 43(6), 1779-1793.
18. Byrom D. (1987) Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology 5(9), 246-250.
19. Carole T. M., Pellegrino J., Paster M. D. (2004) Opportunities in the industrial biobased products industry. Applied Biochemistry and Biotechnology 113, 871-885.
20. Carta F., Beun J. J., van Loosdrecht M. C. M., Heijnen J. J. (2001) Simultaneous storage and degradation of PHB and glycogen in activated sludge cultures. Water Research 35(11), 2693-2701.
21. Cech J. S., Chudoba J. (1983) Influence of accumulation capacity of activated-sludge microorganisms on kinetics of glucose removal. Water Research 17(6), 659-666.
22. Cech J. S., Hartman P. (1990) Glucose-induced break down of enhanced biological phosphate removal. Environmental Technology 11(7), 651-656.
23. Cech J. S., Hartman P. (1993) Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems. Water Research 27(7), 1219-1225.
24. Choi J. I., Lee S. Y. (1997) Process analysis and economic evaluation for poly(3- hydroxybutyrate) production by fermentation. Bioprocess Engineering 17(6), 335-342.
25. Choi J. I., Lee S. Y. (1999a) Efficient and economical recovery of poly(3-hydroxybutyrate) from recombinant Escherichia coli by simple digestion with chemicals. Biotechnology and Bioengineering 62(5), 546-553.
26. Choi J. I., Lee S. Y. (1999b) High-level production of poly(3-hydroxybutyrate-co-3- hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli. Applied and Environmental Microbiology 65(10), 4363-4368.
27. Chua A. S. M., Takabatake H., Satoh H., Mino T. (2003) Production of polyhydroxyalkanoates (PHA) by activated sludge treating municipal wastewater: effect of pH, sludge retention time (SRT), and acetate concentration in influent. Water Research 37(15), 3602-3611.
28. Coats E. R., Loge F. J., Smith W. A., Thompson D. N., Wolcott M. P. (2007a) Functional stability of a mixed microbial consortium producing PHA from waste carbon sources. Applied Biochemistry and Biotechnology 137, 909-925.
29. Coats E. R., Loge F. J., Wolcott M. P., Englund K., McDonald A. G. (2007b) Synthesis of polyhydroxyalkanoates in municipal wastewater treatment. Water Environment Research 79(12), 2396-2403.
30. Dai Y., Yuan Z. G., Wang X. L., Oehmen A., Keller J. (2007) Anaerobic metabolism of Defluviicoccus vanus related glycogen accumulating organisms (GAOs) with acetate and propionate as carbon sources. Water Research 41(9), 1885-1896.
31. Dai Y., Lambert L., Yuan Z., Keller J. (2008) Characterisation of polyhydroxyalkanoate copolymers with controllable four-monomer composition. Journal of Biotechnology 134(1-2), 137-145.
32. Dias J. M. L., Lemos P. C., Serafim L. S., Oliveira C., Eiroa M., Albuquerque M. G. E., Ramos A. M., Oliveira R., Reis M. A. M. (2006) Recent advances in polyhydroxyalkanoate production by mixed aerobic cultures: from the substrate to the final product. Macromolecular Bioscience 6(11), 885-906.
33. Din M. F. M., Ujang Z., van Loosdrecht M. C. M., Ahmad A., Sairan M. F. (2006) Optimization of nitrogen and phosphorus limitation for better biodegradable plastic production and organic removal using single fed-batch mixed cultures and renewable resources. Water Science and Technology 53(6), 15-20.
34. Dionisi D., Majone M., Papa V., Beccari M. (2004a) Biodegradable polymers from organic acids by using activated sludge enriched by aerobic periodic feeding. Biotechnology and Bioengineering 85(6), 569-579.
35. Dionisi D., Renzi V., Majone M., Beccari M., Ramadori R. (2004b) Storage of substrate mixtures by activated sludges under dynamic conditions in anoxic or aerobic environments. Water Research 38(8), 2196-2206.
36. Dionisi D., Beccari M., Di Gregorio S., Majone M., Papini M. P., Vallini G. (2005a) Storage of biodegradable polymers by an enriched microbial community in a sequencing batch reactor operated at high organic load rate. Journal of Chemical Technology and Biotechnology 80(11), 1306-1318.
37. Dionisi D., Carucci G., Papini M. P., Riccardi C., Majone M., Carrasco F. (2005b) Olive oil mill effluents as a feedstock for production of biodegradable polymers. Water Research 39(10), 2076-2084.
38. Dionisi D., Majone M., Levantesi C., Bellani A., Fuoco A. (2006a) Effect of feed length on settleability, substrate uptake and storage in a sequencing batch reactor treating an industrial wastewater. Environmental Technology 27(8), 901-908.
39. Dionisi D., Majone M., Vallini G., Di Gregorio S., Beccari M. (2006b) Effect of the applied organic load rate on biodegradable polymer production by mixed microbial cultures in a sequencing batch reactor. Biotechnology and Bioengineering 93(1), 76-88.
40. Dircks K., Henze M., van Loosdrecht M. C. M., Mosbaek H., Aspegren H. (2001) Storage and degradation of poly-beta-hydroxybutyrate in activated sludge under aerobic conditions. Water Research 35(9), 2277-2285.
41. Du G. C., Chen J., Yu J., Lun S. Y. (2001) Kinetic studies on poly-3-hydroxybutyrate formation by Ralstonia eutropha in a two-stage continuous culture system. Process Biochemistry 37(3), 219-227.
42. Eggink G., Preusting H., Huijberts G., Huisman G., Witholt B. 1992. Synthesis of poly-3- hydroxy-alkanoates (phas) by pseudomonase-substrates polymerases, bioreactor configurations, and products. Abstracts of Papers of the American Chemical Society 204, 73-PMSE.
43. Gaudin P. (1998) Contribution de la synthèse de poly-b-hydroxybutyrate (PHB) á la croissance de Ralstonia eutropha. Doctorat, INSA, Toulouse, France.
44. Goel R., Mino T., Satoh H., Matsuo T. (1999) Modeling hydrolysis processes considering intracellular storage. Water Science and Technology 39(1), 97-105.
45. Grothe E., Chisti Y. (2000) Poly(b-hydroxybutyric acid) thermoplastic production by Alcaligenes latus: behavior of fed-batch cultures. Bioprocess and Biosystems Engineering 22(5), 441.
46. Gurieff N. B. (2007) Production of biodegradable polyhydroxyalkanoate polymers using advanced biological wastewater treatment process technology. University of Queensland, Australia.
47. Gurieff N., Lant P. (2007) Comparative life cycle assessment and financial analysis of mixed culture polyhydroxyalkanoate production. Bioresource Technology 98, 3393-3403.
48. Hollender J., van der Krol D., Kornberger L., Gierden E., Dott W. (2002) Effect of different carbon sources on the enhanced biological phosphorus removal in a sequencing batch reactor. World Journal of Microbiology and Biotechnology 18(4), 355-360.
49. Hrabak O. (1992) Industrial-production of poly-beta-hydroxybutyrate. FEMS Microbiology Reviews 103(2-4), 251-255.
50. Johnson K., Jiang Y., Kleerebezem R., Muyzer G., van Loosdrecht M. C. M. (2009) Enrichment of a mixed bacterial culture with a high polyhydroxyalkanoate storage capacity. Biomacromolecules 10(4), 670-676.
51. Johnson K., Kleerebezem R., van Loosdrecht M. C. M. (2010a) Influence of the C/N ratio on the performance of polyhydroxybutyrate (PHB) producing sequencing batch reactors at short SRTs. Water Research 44(7), 2141-2152.
52. Johnson K., van Geest J., Kleerebezem R., van Loosdrecht M. C. M. (2010b) Short- and longterm temperature effects on aerobic polyhydroxybutyrate producing mixed cultures. Water Research 44(6), 1689-1700.
53. Kapritchkoff F. M., Viotti A. P., Alli R. C. P., Zuccolo M., Pradella J. G. C., Maiorano A. E., Miranda E. A., Bonomi A. (2006) Enzymatic recovery and purification of polyhydroxybutyrate produced by Ralstonia eutropha. Journal of Biotechnology 122(4), 453-462.
54. Kim B. S., Lee S. C., Lee S. Y., Chang H. N., Chang Y. K., Woo S. I. (1994) Production of poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) by fed-batch culture of Alcaligeneseutrophus with substrate control using online glucose analyzer. Enzyme and Microbial Technology 16(7), 556-561.
55. Kim D. Y., Kim H. W., Chung M. G., Rhee Y. H. (2007) Biosynthesis, modification, and biodegradation of bacterial medium-chain-length polyhydroxyalkanoates. Journal of Microbiology 45(2), 87-97.
56. Kleerebezem R., van Loosdrecht M. C. M. (2007) Mixed culture biotechnology for bioenergy production. Current Opinion in Biotechnology 18(3), 207-212.
57. Koller M., Salerno A., Dias M., Reiterer A., Braunegg G. (2010) Modern biotechnological polymer synthesis: a review. Food Technology and Biotechnology 48(3), 255-269.
58. Kothuis B., Schelleman F. (1998). Environmental economic comparison of biotechnology with traditional alternatives. In: Meesters KHP, editor. Production of poly-(3-hydroxyalkanoates) from waste streams. Delft: Tudelft Press; p. 4.
59. Lee I. Y., Kim M. K., Chang H. N., Park Y. H. (1994) Effects of propionate on accumulation of poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) and excretion of pyruvate in Alcaligenes-eutrophus. Biotechnology Letters 16(6), 611-616.
60. LeeS.Y. (1996a) Bacterialpolyhydroxyalkanoates. BiotechnologyandBioengineering49(1),1-14.
61. Lee S. Y. (1996b) Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria. Trends in Biotechnology 14(11), 431-438.
62. Lee S. Y. (1997) E. coli moves into the plastic age. Nature Biotechnology 15(1), 17-18.
63. Lee S. Y., Choi J. I. (1998) Effect of fermentation performance on the economics of poly(3- hydroxybutyrate) production by Alcaligenes latus. Polymer Degradation and Stability 59(1-3), 387-393.
64. Lee S. Y., Choi J-I. (1999) Production and degradation of polyhydroxyalkanoates in waste environment. Waste Management 19(2), 133.
65. Lee S. Y., Choi J. I.,Wong H. H. (1999) Recent advances in polyhydroxyalkanoate production by bacterial fermentation: mini-review. International Journal of Biological Macromolecules 25(1-3), 31-36.
66. Lee Y., Lee S. Y. (1996) Enhanced production of poly(3-hydroxybutyrate) by filamentationsuppressed recombinant Escherichia coli in a defined medium. Journal of Environmental Polymer Degradation 4(2), 131-134.
67. Lemos P. C., Serafim L. S., Reis M. A. M. (2006) Synthesis of polyhydroxyalkanoates from different short-chain fatty acids by mixed cultures submitted to aerobic dynamic feeding. Journal of Biotechnology 122(2), 226-238.
68. Li R., Zhang H. X., Qi Q. S. (2007) The production of polyhydroxyalkanoates in recombinant Escherichia coli. Bioresource Technology 98(12), 2313-2320.
69. Liu H. Y., Hall P. V., Darby J. L., Coats E. R., Green P. G., Thompson D. E., Loge F. J. (2008) Production of polyhydroxyalkanoate during treatment of tomato cannery wastewater.Water Environment Research 80(4), 367-372.
70. Lopez-Vazquez C. M., Song Y. I., Hooijmans C. M., Brdjanovic D., Moussa M. S., Gijzen H. J., van Loosdrecht M. C. M. (2007) Short-term temperature effects on the anaerobic metabolism of glycogen accumulating organisms. Biotechnology and Bioengineering 97(3), 483-495.
71. Majone M., Massanisso P., Carucci A., Lindrea K., Tandoi V. (1996) Influence of storage on kinetic selection to control aerobic filamentous bulking. Water Science and Technology 34(5-6), 223-232.
72. Majone M., Dircks K., Beun J. J. (1999) Aerobic storage under dynamic conditions in activated sludge processes: the state of the art. Water Science and Technology 39(1), 61-73.
73. Majone M., Beccari M., Dionisi D., Levantesi C., Renzi V. (2001) Role of storage phenomena on removal of different substrates during pre-denitrification. Water Science and Technology 43(3), 151-158.
74. Mato T., Ben M., Kennes C., Veiga M. C. (2008) PHA production using brewery wastewater. 4th Sequencing Batch Reactor Conference Rome, April 7-10, pp. 59-66.
75. Mengmeng C., Hong C., Qingliang Z., Shirley S. N., Jie R. (2009) Optimal production of polyhydroxyalkanoates (PHA) in activated sludge fed by volatile fatty acids (VFAs) generated from alkaline excess sludge fermentation. Bioresource Technology 100(3), 1399-1405.
76. Mino T., Van Loosdrecht M. C. M., Heijnen J. J. (1998) Microbiology and biochemistry of the enhanced biological phosphate removal process. Water Research 32(11), 3193-3207.
77. Moo-Young M., Tamer L. M., Chisti Y. (1998) A process for low-cost recovery of microbially produced poly(beta-hydroxybutyric acid) thermoplastic. Abstracts of Papers of the American Chemical Society 216, 185-BIOT.
78. Odegaard H., Gisvold B., Helness H., Sjovold F., Zuliang L. (2000) High rate biological/ chemical treatment based on the moving bed biofilm process combined with coagulation. Chemical Water and Wastewater Treatment 6, 245-255.
79. Oehmen A., Lemos P. C., Carvalho G., Yuan Z. G., Keller J., Blackall L. L., Reis M. A. M. (2007) Advances in enhanced biological phosphorus removal: from micro to macro scale. Water Research 41(11), 2271-2300.
80. Page W. J., Knosp O. (1989) Hyperproduction of poly-beta-hydroxybutyrate during exponential- growth of Azotobacter-vinelandiiuwd. Applied Environmental Microbiology 55(6), 1334-1339.
81. Paul E. (2010). PHB production under phosphorus limitation in fed batch culture inoculated directly with activated sludge. Unpublished report, INSA, Toulouse, France.
82. Paul E., Wolff D. B., Ochoa J. C., da Costa R. H. R. (2007) Recycled and virgin plastic carriers in hybrid reactors for wastewater treatment. Water Environment Research 79(7), 765-774.
83. Pisco A. R., Bengtsson S.,Werker A., Reis M. A. M., Lemos P. C. (2008) Use of industrial byproducts for polyhydroxyalkanoates production by glycogen-accumulating organisms. Proceedings of the 5th IWA Leading-Edge Conference on Water and Wastewater Technologies, Zurich, Switzerland, June, 1-4.
84. Pisco A. R., Bengtsson S.,Werker A., ReisM. A.M., Lemos P. C. (2009) Community structure evolution and enrichment of glycogen-accumulating organisms producing polyhydroxyalkanoates from fermented molasses. Applied Environmental Microbiology 75(14), 4676-4686.
85. Reddy C. S. K., Ghai R., Kalia V. C. (2003) Polyhydroxyalkanoates: an overview. Bioresource Technology 87(2), 137-146.
86. Reis M. A. M., SerafimL. S., Lemos P. C., Ramos A. M., Aguiar F. R., van Loosdrecht M. C. M. (2003) Production of polyhydroxyalkanoates by mixed microbial cultures. Bioprocess and Biosystems Engineering 25(6), 377-385.
87. Rhu D. H., LeeW. H., Kim J. Y., Choi E. (2003) Polyhydroxyalkanoate (PHA) production from waste. Water Science and Technology 48(8), 221-228.
88. Salehizadeh H., van Loosdrecht M. C. M. (2004) Production of polyhydroxyalkanoates by mixed culture: recent trends and biotechnological importance. Biotechnology Advances 22(3), 261-279.
89. Satoh H., Mino T., Matsuo T. (1992) Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal processes. Water Science and Technology 26(5-6), 933-942.
90. Satoh H., Mino T., Matsuo T. (1994) Deterioration of enhanced biological phosphorus removal by the domination of microorganisms without polyphosphate accumulation. Water Science and Technology 30(6), 203-211.
91. Satoh H., Iwamoto Y., Mino T., Matsuo T. (1998a) Activated sludge as a possible source of biodegradable plastic. Water Science and Technology 38(2), 103-109.
92. Satoh H., Ramey W. D., Koch F. A., Oldham W. K., Mino T., Matsuo T. (1996) Anaerobic substrate uptake by the enhanced biological phosphorus removal activated sludge treating real sewage. Water Science and Technology 34(1-2), 9-16.
93. Satoh H., Mino T., Matsuo T. (1998b) Anaerobic uptake of glutamate and aspartate by enhanced biological phosphorus removal activated sludge. Water Science and Technology 37(4-5), 579-582.
94. Satoh H., Mino T., Matsuo T. (1999) PHA production by activated sludge. International Journal of Biological Macromolecules 25(1-3), 105-109.
95. Senior P. J., Dawes E. A. (1971) Poly-beta-hydroxybutyrate biosynthesis and regulation of glucose metabolim in Azotobacter-beijerinckii. Biochemical Journal 125(1), 55-66.
96. Serafim L. S., Lemos P. C., Oliveira R., Ramos A. M., Reis M. A. M. (2004a) High storage of PHB by mixed microbial cultures under aerobic dynamic feeding conditions. European Symposium on Environmental Biotechnology, pp. 479-482.
97. Serafim L. S., Lemos P. C., Oliveira R., Reis M. A. M. (2004b) Optimization of polyhydroxybutyrate production by mixed cultures submitted to aerobic dynamic feeding conditions. Biotechnology and Bioengineering 87(2), 145-160.
98. SerafimL. S., Lemos P. C., Rossetti S., Levantesi C., TandoiV., Reis M. A. M. (2006) Microbial community analysis with a high PHA storage capacity.Water Science and Technology 54(1), 183-188.
99. Serafim L. S., Lemos P. C., Albuquerque M. G. E., Reis M. A. M. (2008) Strategies for PHA production by mixed cultures and renewable waste materials. Applied Microbiology and Biotechnology 81(4), 615-628.
100. Takabatake H., Satoh H., Mino T., Matsuo T. (2000) Recovery of biodegradable plastics from activated sludge process. Water Science and Technology 42(3-4), 351-356.
101. Tamer I. M., Moo-Young M., Chisti Y. (1998a) Disruption of Alcaligenes latus for recovery of poly(beta-hydroxybutyric acid): Comparison of high-pressure homogenization, beadmilling, and chemically induced lysis. Industrial and Engineering Chemistry Research 37(5), 1807-1814.
102. Tamer I. M., Moo-Young M., Chisti Y. (1998b) Optimization of poly(beta-hydroxybutyric acid) recovery from Alcaligenes latus: combined mechanical and chemical treatments. Bioprocess Engineering 19(6), 459-468.
103. Tohyama M., Shimizu K. (1999) Control of a mixed culture of Lactobacillus delbrueckii and Ralstonia eutropha for the production of PHB from glucose via lactate. Biochemical Engineering Journal 4(1), 45.
104. Tsuge T., Tanaka K., Ishizaki A. (2001) Development of a novel method for feeding a mixture of L-lactic acid and acetic acid in fed-batch culture of Ralstonia eutropha for poly-D-3- hydroxybutyrate production. Journal of Bioscience and Bioengineering 91(6), 545-550.
105. van Aalastvan Leeuwen M. A., Pot M. A., van Loosdrecht M. C. M., Heijnen J. J. (1997) Kinetic modeling of poly(beta-hydroxybutyrate) production and consumption by Paracoccus pantotrophus under dynamic substrate supply. Biotechnology and Bioengineering 55(5), 773-782.
106. van Loosdrecht M. C. M., Pot M. A., Heijnen J. J. (1997) Importance of bacterial storage polymers in bioprocess. Water Science and Technology 35, 41-47.
107. Vollbrecht D., Schlegel H. G. (1979) Excretion of metabolites of hydrogen bacteria: 3. D(-)-3- hydroxybutanoate. European Journal of Applied Microbiology and Biotechnology 7(3), 259-266.
108. Wallen L., Rohwedder W. (1974) Poly-b-hydroxyalkanoate from activated sludge. Environment Science and Technology 8, 576-579.
109. Wang F., Lee S. Y. (1997) Poly(3-hydroxybutyrate) production with high productivity and high polymer content by a fed-batch culture of Alcaligenes latus under nitrogen limitation. Applied Environmental Microbiology 63(9), 3703-3706.
110. Witholt B.,Kessler B. (1999) Perspectives of medium chain length poly (hydroxyalkanoates), a versatile set of bacterial bioplastics. Current Opinion in Biotechnology 10(3), 279-285.
111. Yu J. (2001) Production of PHA from starchy wastewater via organic acids. Journal of Biotechnology 86, 105-112.