Lysis-on-chip of single target cells following forced interaction with CTLs or NK cells on a dielectrophoresis-based array

Journal of Immunology

Volumn 191, Issue 7, 2013, Pages 3545-3552

  Abonnenc, Melanie ^a   Borgatti, Monica ^b   Fabbri, Enrica ^b   Gavioli, Riccardo ^b   Fortini, Cinzia ^b   Destro, Federica ^b   Altomare, Luigi ^c   Manaresi, Nicolò ^a   Medoro, Gianni ^a   Romani, Aldo ^c   Tartagni, Marco ^c   Monaco, Elisa Lo ^d   Giacomini, Patrizio ^d   Guerrieri, Roberto ^c   Gambari, Roberto ^b  

^a Silicon Biosystems   (Italy)

^b UNIVERSITY OF FERRARA   (Italy)

^c UNIVERSITY OF BOLOGNA   (Italy)

^d REGINA ELENA NATIONAL CANCER INSTITUTE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CALCEIN;

ARTICLE; CELL INTERACTION; CELL MEDIATED CYTOTOXICITY; CONTROLLED STUDY; CYTOTOXIC T LYMPHOCYTE; EFFECTOR CELL; ELECTROPHORESIS; HUMAN; HUMAN CELL; LAB ON A CHIP; NATURAL KILLER CELL; PRIORITY JOURNAL; TUMOR CELL;

CELL COMMUNICATION; CELL LINE, TRANSFORMED; CELL LINE, TUMOR; CELL MEMBRANE PERMEABILITY; CYTOTOXICITY, IMMUNOLOGIC; HUMANS; KILLER CELLS, NATURAL; SINGLE-CELL ANALYSIS; T-LYMPHOCYTES, CYTOTOXIC;

EID: 84885092328     PISSN: 00221767     EISSN: 15506606     Source Type: Journal    
DOI: 10.4049/jimmunol.1300890     Document Type: Article

References (45)

1. Kricka, L. J. 2001. Microchips, microarrays, biochips and nanochips: personal laboratories for the 21st century. Clin. Chim. Acta 307:219-223.
2. Hughes, M. P. 2002. Strategies for dielectrophoretic separation in laboratory-ona-chip systems. Electrophoresis 23:2569-2582.
3. Müller, T., A. Pfennig, P. Klein, G. Gradl, M. Jäger, and T. Schnelle. 2003. The potential of dielectrophoresis for single-cell experiments. IEEE Eng. Med. Biol. Mag. 22:51-61.
4. Gascoyne, P. R., J. V. Vykoukal, J. A. Schwartz, T. J. Anderson, D. M. Vykoukal, K. W. Current, C. McConaghy, F. F. Becker, and C. Andrews. 2004. Dielectrophoresis-based programmable fluidic processors. Lab Chip 4:299-309.
5. Fatoyinbo, H. O., D. Kamchis, R. Whattingham, S. L. Ogin, and M. P. Hughes. 2005. A high-throughput 3-D composite dielectrophoretic separator. IEEE Trans. Biomed. Eng. 52:1347-1349.
6. Lin, R. Z., C. T. Ho, C. H. Liu, and H. Y. Chang. 2006. Dielectrophoresis basedcell patterning for tissue engineering. Biotechnol. J. 1:949-957.
7. Manaresi, N., A. Romani, G. Medoro, L. Altomare, A. Leonardi, M. Tartagni, and R. Guerrieri. 2003. A CMOS chip for individual cell manipulation and detection. IEEE J. Solid-State Circuits 38:2297-2305.
8. Medoro, G., C. Nastruzzi, R. Guerrieri, R. Gambari, and N. Manaresi. 2007. Lab on a chip for live-cell manipulation. IEEE Design Test Computers 24:26-36.
9. Wong, P. K., C. Y. Chen, T. H. Wang, and C. M. Ho. 2004. Electrokinetic bioprocessor for concentrating cells and molecules. Anal. Chem. 76:6908-6914.
10. Hunt, T. P., H. Lee, and R. M. Westervelt. 2004. Addressable micropost array for the dielectrophoretic manipulation of particles in fluid. Appl. Phys. Lett. 85:6421-6423.
11. Fiedler, S., S. G. Shirley, T. Schnelle, and G. Fuhr. 1998. Dielectrophoretic sorting of particles and cells in a microsystem. Anal. Chem. 70:1909-1915.
12. Xu, C., Y. Wang, M. Cao, and Z. Lu. 1999. Dielectrophoresis of human red cells in microchips. Electrophoresis 20:1829-1831.
13. Altomare, L., M. Borgatti, G. Medoro, N. Manaresi, M. Tartagni, R. Guerrieri, and R. Gambari. 2003. Levitation and movement of human tumor cells using a printed circuit board device based on software-controlled dielectrophoresis. Biotechnol. Bioeng. 82:474-479.
14. Micheletti, F., A. Canella, S. Vertuani, M. Marastoni, L. Tosi, S. Volinia, S. Traniello, and R. Gavioli. 2000. Supra-agonist peptides enhance the reactivation of memory CTL responses. J. Immunol. 165:4264-4271.
15. Navarro, F., M. Llano, T. Bellón, M. Colonna, D. E. Geraghty, and M. López-Botet. 1999. The ILT2 (LIR1) and CD94/NKG2A NK cell receptors respectively recognize HLA-G1 and HLA-E molecules co-expressed on target cells. Eur. J. Immunol. 29:277-283.
16. Shimizu, Y., D. E. Geraghty, B. H. Koller, H. T. Orr, and R. De Mars. 1988. Transfer and expression of three cloned human non-HLA-A, B, C class I major histocompatibility complex genes in mutant lymphoblastoid cells. Proc. Natl. Acad. Sci. USA 85:227-231.
17. Chumbley, G., A. King, K. Robertson, N. Holmes, and Y. W. Loke. 1994. Resistance of HLA-G and HLA-A2 transfectants to lysis by decidual NK cells. Cell. Immunol. 155:312-322.
18. Robertson, M. J., K. J. Cochran, C. Cameron, J. M. Le, R. Tantravahi, and J. Ritz. 1996. Characterization of a cell line, NKL, derived from an aggressive human natural killer cell leukemia. Exp. Hematol. 24:406-415.
19. Manaresi, N. 2003. A CMOS Chip for individual cell manipulation and detection. ISSCC 487:192-193.
20. Gambari, R., M. Borgatti, L. Altomare, N. Manaresi, G. Medoro, A. Romani, M. Tartagni, and R. Guerrieri. 2003. Applications to cancer research of "lab-ona-chip" devices based on dielectrophoresis (DEP). Technol. Cancer Res. Treat. 2:31-40.
21. Borgatti, M., L. Altomare, M. Abonnec, E. Fabbri, N. Manaresi, G. Medoro, A. Romani, M. Tartagni, C. Nastruzzi, S. Di Croce, et al. 2005. Dielectrophoresisbased 'Lab-on-a-chip' devices for programmable binding of microspheres to target cells. Int. J. Oncol. 27:1559-1566.
22. Lichtenfels, R., W. E. Biddison, H. Schulz, A. B. Vogt, and R. Martin. 1994. CARE-LASS (calcein-release-assay), an improved fluorescence-based test system to measure cytotoxic T lymphocyte activity. J. Immunol. Methods 172:227-239.
23. Jayaraman, S. 2005. Flow cytometric determination of mitochondrial membrane potential changes during apoptosis of T lymphocytic and pancreatic beta cell lines: comparison of tetramethylrhodamineethylester (TMRE), chloromethyl-X-rosamine (H2-CMX-Ros) and MitoTracker Red 580 (MTR580). J. Immunol. Methods 306:68-79.
24. Neri, S., E. Mariani, A. Meneghetti, L. Cattini, and A. Facchini. 2001. Calceinacetyoxymethyl cytotoxicity assay: standardization of a method allowing additional analyses on recovered effector cells and supernatants. Clin. Diagn. Lab. Immunol. 8:1131-1135.
25. Roden, M. M., K. H. Lee, M. C. Panelli, and F. M. Marincola. 1999. A novel cytolysis assay using fluorescent labeling and quantitative fluorescent scanning technology. J. Immunol. Methods 226:29-41.
26. Wallace, D., A. Hildesheim, and L. A. Pinto. 2004. Comparison of benchtop microplate beta counters with the traditional gamma counting method for measurement of chromium-51 release in cytotoxic assays. Clin. Diagn. Lab. Immunol. 11:255-260.
27. Hillman, G. G., N. Roessler, R. S. Fulbright, J. E. Pontes, and G. P. Haas. 1993. 51Cr-release assay adapted to a 96-well format sample reading. Biotechniques 15:744-749.
28. Nagao, F., T. Yabe, M. Xu, K. Yokoyama, K. Saito, and K. Okumura. 1996. Application of non-radioactive europium (Eu3+) release assay to a measurement of human natural killer activity of healthy and patient populations. Immunol. Invest. 25:507-518.
29. Bouma, G. J., P. M. van der Meer-Prins, F. P. van Bree, J. J. van Rood, and F. H. Claas. 1992. Determination of cytotoxic T-lymphocyte precursor frequencies using europium labeling as a nonradioactive alternative to labeling with chromium-51. Hum. Immunol. 35:85-92.
30. Gharehbaghian, A., K. M. Haque, C. Truman, J. Newman, and B. A. Bradley. 2002. Quantitation of natural killer cell precursors in man. J. Immunol. Methods 260:69-77.
31. Suhrbier, A., A. Fernan, S. R. Burrows, A. Saul, and D. J. Moss. 1991. BLT esterase activity as an alternative to chromium release in cytotoxic T cell assays. J. Immunol. Methods 145:43-53.
32. Bachy, M., A. Bonnin-Rivalland, V. Tilliet, and E. Trannoy. 1999. Beta galactosidase release as an alternative to chromium release in cytotoxic T-cell assays. J. Immunol. Methods 230:37-46.
33. Goldberg, J. E., S. W. Sherwood, and C. Clayberger. 1999. A novel method for measuring CTL and NK cell-mediated cytotoxicity using annexin V and twocolor flow cytometry. J. Immunol. Methods 224:1-9.
34. Shotton, D. M., and A. Attaran. 1998. Variant antigenic peptide promotes cytotoxic T lymphocyte adhesion to target cells without cytotoxicity. Proc. Natl. Acad. Sci. USA 95:15571-15576.
35. Stinchcombe, J. C., G. Bossi, S. Booth, and G. M. Griffiths. 2001. The immunological synapse of CTL contains a secretory domain and membrane bridges. Immunity 15:751-761.
36. Glamann, J., and A. J. Hansen. 2006. Dynamic detection of natural killer cellmediated cytotoxicity and cell adhesion by electrical impedance measurements. Assay Drug Dev. Technol. 4:555-563.
37. Pittet, M. J., D. Valmori, P. R. Dunbar, D. E. Speiser, D. Liénard, F. Lejeune, K. Fleischhauer, V. Cerundolo, J. C. Cerottini, and P. Romero. 1999. High frequencies of naive Melan-A/MART-1-specific CD8 (+) T cells in a large proportion of human histocompatibility leukocyte antigen (HLA)-A2 individuals. J. Exp. Med. 190:705-715.
38. Stratov, I., R. De Rose, D. F. Purcell, and S. J. Kent. 2004. Vaccines and vaccine strategies against HIV. Curr. Drug Targets 5:71-88.
39. Tsujimura, K., Y. Obata, and T. Takahashi. 2004. Thymus-leukemia antigen (TL) as a major histocompatibility complex (MHC) class Ib molecule and tumorspecific antigen. Cancer Sci. 95:469-474.
40. Dunbar, P. R., G. S. Ogg, J. Chen, N. Rust, P. van der Bruggen, and V. Cerundolo. 1998. Direct isolation, phenotyping and cloning of low-frequency antigen-specific cytotoxic T lymphocytes from peripheral blood. Curr. Biol. 8:413-416.
41. von Zons, P., P. Crowley-Nowick, D. Friberg, M. Bell, U. Koldovsky, and T. L. Whiteside. 1997. Comparison of europium and chromium release assays: cytotoxicity in healthy individuals and patients with cervical carcinoma. Clin. Diagn. Lab. Immunol. 4:202-207.
42. Granberg, C., K. Blomberg, I. Hemmilä, and T. Lövgren. 1988. Determination of cytotoxic T lymphocyte activity by time-resolved fluorometry using europiumlabelled concanavalin A-stimulated cells as targets. J. Immunol. Methods 114:191-195.
43. Blomberg, K., R. Hautala, J. Lövgren, V. M. Mukkala, C. Lindqvist, and K. Akerman. 1996. Time-resolved fluorometric assay for natural killer activity using target cells labelled with a fluorescence enhancing ligand. J. Immunol. Methods 193:199-206.
44. Fruci, D., M. Benevolo, L. Cifaldi, S. Lorenzi, E. Lo Monaco, E. Tremante, and P. Giacomini. 2012. Major histocompatibility complex class i and tumour immuno-evasion: how to fool T cells and natural killer cells at one time. Curr. Oncol. 19:39-41.
45. González, A., V. Rebmann, J. Le Maoult, P. A. Horn, E. D. Carosella, and E. Alegre. 2012. The immunosuppressive molecule HLA-G and its clinical implications. Crit. Rev. Clin. Lab. Sci. 49:63-84.