1.55 Å-resolution structure of ent-copalyl diphosphate synthase and exploration of general acid function by site-directed mutagenesis

Biochimica et Biophysica Acta - General Subjects

Volumn 1840, Issue 1, 2014, Pages 184-190

  Köksal, Mustafa ^a,c   Potter, Kevin ^b   Peters, Reuben J ^b   Christianson, David W ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b IOWA STATE UNIVERSITY   (United States)

^c IZMIR INSTITUTE OF TECHNOLOGY   (Turkey)

Author keywords

Enzyme mechanism; Gibberellin biosynthesis; Protein crystallography; Terpene cyclase

Indexed keywords

ACID; AMMONIA; COPALYL DIPHOSPHATE SYNTHASE; DIPHOSPHATE GROUP; MAGNESIUM ION; MUTANT PROTEIN; PHOSPHATE; PROTON; SOLVENT; SYNTHETASE; UNCLASSIFIED DRUG; WATER; ASPARTIC ACID; ENT-KAURENE SYNTHETASE A; GERANYLGERANYL PYROPHOSPHATE; ISOPRENOID PHOSPHATE; TRANSFERASE; VEGETABLE PROTEIN; ENT KAURENE SYNTHETASE A;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; CRYSTALLIZATION; DIFFRACTION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; HYDROGEN BOND; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; ARABIDOPSIS; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY; ENZYME MECHANISM; GIBBERELLIN BIOSYNTHESIS; PROTEIN CRYSTALLOGRAPHY; TERPENE CYCLASE;

ALKYL AND ARYL TRANSFERASES; ARABIDOPSIS; ASPARTIC ACID; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; HYDROGEN BONDING; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLANT PROTEINS; POLYISOPRENYL PHOSPHATES; PROTEIN CONFORMATION; PROTONS;

ENZYME MECHANISM; GIBBERELLIN BIOSYNTHESIS; PROTEIN CRYSTALLOGRAPHY; TERPENE CYCLASE;

EID: 84885074387     PISSN: 03044165     EISSN: 18728006     Source Type: Journal    
DOI: 10.1016/j.bbagen.2013.09.004     Document Type: Article

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