Conditional Gene Expression in Chlamydia trachomatis Using the Tet System

PLoS ONE

Volumn 8, Issue 10, 2013, Pages

  Wickstrum, Jason ^a   Sammons, Lindsay R ^a   Restivo, Keasha N ^a   Hefty, P Scott ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANHYDROTETRACYCLINE; GREEN FLUORESCENT PROTEIN; TETRACYCLINE;

ARTICLE; BACTERIOLOGY; CHLAMYDIA TRACHOMATIS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DEVELOPMENTAL STAGE; GENE CONTROL; NONHUMAN; PATHOGENESIS; PROTEIN EXPRESSION; PROTEIN INDUCTION; REPRESSOR GENE; SHUTTLE VECTOR;

ANIMALS; ANTIPORTERS; BACTERIAL PROTEINS; CELL LINE, TUMOR; CHLAMYDIA TRACHOMATIS; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MICE; MICROSCOPY, FLUORESCENCE; PROMOTER REGIONS, GENETIC; REPRESSOR PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TETRACYCLINE RESISTANCE; TETRACYCLINES;

EID: 84885053267     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0076743     Document Type: Article

References (33)

1. Stephens RS, (2003) The cellular paradigm of chlamydial pathogenesis. Trends Microbiol 11: 44-51. doi:10.1016/S0966-842X(02)00011-2. PubMed: 12526854.
2. Abdelrahman YM, Belland RJ, (2005) The chlamydial developmental cycle. FEMS Microbiol Rev 29: 949-959. doi:10.1016/j.femsre.2005.03.002. PubMed: 16043254.
3. Hybiske K, Stephens RS, (2007) Mechanisms of host cell exit by the intracellular bacterium Chlamydia. Proc Natl Acad Sci U S A 104: 11430-11435. doi:10.1073/pnas.0703218104. PubMed: 17592133.
4. Wang Y, Kahane S, Cutcliffe LT, Skilton RJ, Lambden PR, et al. (2011) Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector. PLOS Pathog 7: e1002258. PubMed: 21966270.
5. Agaisse H, Derré I, (2013) A C. trachomatis cloning vector and the generation of C. trachomatis strains expressing fluorescent proteins under the control of a C. trachomatis promoter. PLOS ONE 8: e57090. doi:10.1371/journal.pone.0057090. PubMed: 23441233.
6. Voigt CA, (2006) Genetic parts to program bacteria. Curr Opin Biotechnol 17: 548-557. doi:10.1016/j.copbio.2006.09.001. PubMed: 16978856.
7. Bertram R, Hillen W, (2008) The application of Tet repressor in prokaryotic gene regulation and expression. J Microbiol Biotechnol 1: 2-16.
8. Hillen W, Berens C, (1994) Mechanisms underlying expression of Tn10 encoded tetracycline resistance. Annu Rev Microbiol 48: 345-369. doi:10.1146/annurev.mi.48.100194.002021. PubMed: 7826010.
9. Hernandez-Abanto SM, Woolwine SC, Jain SK, Bishai WR, (2006) Tetracycline-inducible gene expression in mycobacteria within an animal host using modified Streptomyces tcp830 regulatory elements. Arch Microbiol 186: 459-464. doi:10.1007/s00203-006-0160-2. PubMed: 16944099.
10. Whetstine CR, Slusser JG, Zückert WR, (2009) Development of a single-plasmid-based regulatable gene expression system for Borrelia burgdorferi. Appl Environ Microbiol 75: 6553-6558. doi:10.1128/AEM.02825-08. PubMed: 19700541.
11. Lutz R, Bujard H, (1997) Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res 25: 1203-1210. doi:10.1093/nar/25.6.1203. PubMed: 9092630.
12. Corrigan RM, Foster TJ, (2009) An improved tetracycline-inducible expression vector for Staphylococcus aureus. Plasmid 61: 126-129. doi:10.1016/j.plasmid.2008.10.001. PubMed: 18996145.
13. Gossen M, Bujard H, (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A 89: 5547-5551. doi:10.1073/pnas.89.12.5547. PubMed: 1319065.
14. Furth PA, St Onge L, Böger H, Gruss P, Gossen M, et al. (1994) Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc Natl Acad Sci U S A 91: 9302-9306. doi:10.1073/pnas.91.20.9302. PubMed: 7937760.
15. Ji Y, Zhang B, Van SF, Horn Warren Pet al. (2001) Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293: 2266-2269. doi:10.1126/science.1063566. PubMed: 11567142.
16. Degenkolb J, Takahashi M, Ellestad GA, Hillen W, (1991) Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor. Antimicrob Agents Chemother 35: 1591-1595. doi:10.1128/AAC.35.8.1591. PubMed: 1929330.
17. Oliva B, Gordon G, McNicholas P, Ellestad G, Chopra I, (1992) Evidence that tetracycline analogs whose primary target is not the bacterial ribosome cause lysis of Escherichia coli. Antimicrob Agents Chemother 36: 913-919. doi:10.1128/AAC.36.5.913. PubMed: 1510413.
18. Kamionka A, Bogdanska-Urbaniak J, Scholz O, Hillen W, (2004) Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor. Nucleic Acids Res 32: 842-847. doi:10.1093/nar/gkh200. PubMed: 14764926.
19. Song L, Carlson JH, Whitmire WM, Kari L, Virtaneva K, et al. (2013) Chlamydia trachomatis plasmid-encoded Pgp4 is a transcriptional regulator of virulence-associated genes. Infect Immun 81: 636-644. doi:10.1128/IAI.01305-12. PubMed: 23319558.
20. Wang Y, Cutcliffe LT, Skilton RJ, Persson K, Bjartling C, et al. (2013) Transformation of a plasmid-free, genital tract isolate of Chlamydia trachomatis with a plasmid vector carrying a deletion in CDS6 revealed that this gene regulates inclusion phenotype. Pathog Dis 67: 100-103. doi:10.1111/2049-632X.12024. PubMed: 23620154.
21. Wang Y, Kahane S, Cutcliffe LT, Skilton RJ, Lambden PR, et al. (2013) Genetic transformation of a clinical (genital tract), plasmid-free isolate of Chlamydia trachomatis: engineering the plasmid as a cloning vector. PLOS ONE 8: e59195. doi:10.1371/journal.pone.0059195. PubMed: 23527131.
22. Skerra A, (1994) A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments. Gene 141: 79-84. doi:10.1016/0378-1119(94)90131-7. PubMed: 8163179.
23. Heim R, Tsien RY, (1996) Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol 6: 178-182. doi:10.1016/S0960-9822(02)00450-5. PubMed: 8673464.
24. Osaka I, Hills JM, Kieweg SL, Shinogle HE, Moore DS, et al. (2012) An automated image-based method for rapid analysis of Chlamydia infection as a tool for screening antichlamydial agents. Antimicrob Agents Chemother 56: 4184-4188. doi:10.1128/AAC.00427-12. PubMed: 22615279.
25. Osaka I, Hefty PS, (2013) Simple resazurin-based microplate assay for measuring Chlamydia infections. Antimicrob Agents Chemother 57: 2838-2840. doi:10.1128/AAC.00056-13. PubMed: 23507273.
26. Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, et al. (2009) Far-red fluorescent tags for protein imaging in living tissues. Biochem J 418: 567-574. doi:10.1042/BJ20081949. PubMed: 19143658.
27. Lambden PR, Pickett MA, Clarke IN, (2006) The effect of penicillin on Chlamydia trachomatis DNA replication. Microbiology 152: 2573-2578. doi:10.1099/mic.0.29032-0. PubMed: 16946252.
28. Mathews SA, Volp KM, Timms P, (1999) Development of a quantitative gene expression assay for Chlamydia trachomatis identified temporal expression of sigma factors. FEBS Lett 458: 354-358. doi:10.1016/S0014-5793(99)01182-5. PubMed: 10570939.
29. Wilson DP, Mathews S, Wan C, Pettitt AN, McElwain DL, (2004) Use of a quantitative gene expression assay based on micro-array techniques and a mathematical model for the investigation of chlamydial generation time. Bull Math Biol 66: 523-537. doi:10.1016/j.bulm.2003.09.001. PubMed: 15006447.
30. Gong S, Yang Z, Lei L, Shen L, Zhong G, (2013) Characterization of Chlamydia trachomatis plasmid-encoded open reading frames. J Bacteriol, 195: 3819-26. PubMed: 23794619.
31. Piatkevich KD, Hulit J, Subach OM, Wu B, Abdulla A, et al. (2010) Monomeric red fluorescent proteins with a large Stokes shift. Proc Natl Acad Sci U S A 107: 5369-5374. doi:10.1073/pnas.0914365107. PubMed: 20212155.
32. Schaumburg CS, Tan M, (2003) Mutational analysis of the Chlamydia trachomatis dnaK promoter defines the optimal-35 promoter element. Nucleic Acids Res 31: 551-555. doi:10.1093/nar/gkg150. PubMed: 12527761.
33. Rozen S, Skaletsky H, (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132: 365-386. PubMed: 10547847.