Transformation of a plasmid-free, genital tract isolate of Chlamydia trachomatis with a plasmid vector carrying a deletion in CDS6 revealed that this gene regulates inclusion phenotype

Pathogens and Disease

Volumn 67, Issue 2, 2013, Pages 100-103

  Wang, Yibing ^a   Cutcliffe, Lesley T ^a   Skilton, Rachel J ^a   Persson, Kenneth ^b   Bjartling, Carina ^c   Clarke, Ian N ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b Malmo University Hospital   (Sweden)

^c LUND UNIVERSITY HOSPITAL   (Sweden)

Author keywords

CDS 6; Chlamydia trachomatis; Deletion; Glycogen biosynthesis; Plasmid; Transformation

Indexed keywords

GLYCOGEN; GREEN FLUORESCENT PROTEIN; PLASMID VECTOR;

ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATION; BACTERIUM TRANSFORMATION; CHLAMYDIA TRACHOMATIS; GENE CONTROL; GENE DELETION; GENE FUNCTION; GENETIC TRANSFORMATION; GENITAL SYSTEM; GLYCOGEN METABOLISM; HOST; MICROBIAL MORPHOLOGY; NONHUMAN; PENICILLIN RESISTANCE; PHENOTYPE; PRIORITY JOURNAL; SHUTTLE VECTOR;

BACTERIAL PROTEINS; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE DELETION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; GENITALIA; GLYCOGEN; PHENOTYPE; PLASMIDS; TRANSFORMATION, BACTERIAL;

EID: 84878131291     PISSN: None     EISSN: 2049632X     Source Type: Journal    
DOI: 10.1111/2049-632X.12024     Document Type: Article

References (23)

1. Albrecht M, Sharma CM, Reinhardt R, Vogel J & Rudel T (2010) Deep sequencing-based discovery of the Chlamydia trachomatis transcriptome. Nucleic Acids Res 38: 868-877.
2. Carlson JH, Whitmire WM, Crane DD et al. (2008) The Chlamydia trachomatis plasmid is a transcriptional regulator of chromosomal genes and a virulence factor. Infect Immun 76: 2273-2283.
3. Farencena A, Comanducci M, Donati M, Ratti G & Cevenini R (1997) Characterization of a new isolate of Chlamydia trachomatis which lacks the common plasmid and has properties of Biovar trachoma. Infect Immun 65: 2965-2969.
4. Harris SR, Clarke IN, Seth-Smith HM et al. (2012) Whole-genome analysis of diverse Chlamydia trachomatis strains identifies phylogenetic relationships masked by current clinical typing. Nat Genet 44: 413-419.
5. Hatt C, Ward ME & Clarke IN (1988) Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication. Nucleic Acids Res 16: 4053.
6. Kari L, Whitmire WM, Olivares-Zavaleta N et al. (2011) A live-attenuated chlamydial vaccine protects against trachoma in nonhuman primates. J Exp Med 208: 2217-2223.
7. Kokes M & Valdivia RH (2012) Cell biology of the chlamydial inclusion. Intracellular Pathogens I Chlamydiales (Tan M & Bavoil PM, eds), pp. 170-191. ASM Press, Washington, DC.
8. Lambden PR, Pickett MA & Clarke IN (2006) The effect of penicillin on Chlamydia trachomatis DNA replication. Microbiology 152: 2573-2578.
9. O'Connell CM & Nicks KM (2006) A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture. Microbiology 152: 1601-1607.
10. O'Connell CM, Abdelrahman YM, Green E, Darville HK, Saira K, Smith B, Darville T, Scurlock AM, Meyer CR & Belland RJ (2011) Toll-like receptor 2 activation by Chlamydia trachomatis is plasmid dependent, and plasmid-responsive chromosomal loci are coordinately regulated in response to glucose limitation by C. trachomatis but not by C. muridarum. Infect Immun 79: 1044-1056.
11. Olivares-Zavaleta N, Whitmire W, Gardner D & Caldwell HD (2010) Immunization with the attenuated plasmidless Chlamydia trachomatis L2(25667R) strain provides partial protection in a murine model of female genitourinary tract infection. Vaccine 28: 1454-1462.
12. Persson K, Bredberg A & Bojs G (1996) Two strains of Chlamydia trachomatis without detectable 7.5kb plasmid. Proc Eur Soc Chlamydia Res 3: 33.
13. Peters J, Wilson DP, Myers G, Timms P & Bavoil PM (2007) Type III secretion a la Chlamydia. Trends Microbiol 15: 241-251.
14. Peterson EM, Markoff BA, Schachter J & de la Maza LM (1990) The 7.5-kb plasmid present in Chlamydia trachomatis is not essential for the growth of this microorganism. Plasmid 23: 144-148.
15. Ricci S, Ratti G & Scarlato V (1995) Transcriptional regulation in the Chlamydia trachomatis pCT plasmid. Gene 154: 93-98.
16. Rockey DD (2011) Unraveling the basic biology and clinical significance of the chlamydial plasmid. J Exp Med 208: 2159-2162.
17. Seth-Smith HMB, Harris SR, Persson K et al. (2009) Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain. BMC Genomics 10: 239.
18. Stothard DR, Williams JA, Van der Pol B & Jones RB (1998) Identification of a Chlamydia trachomatis serovar E urogenital isolate which lacks the cryptic plasmid. Infect Immun 66: 6010-6013.
19. Thomas NS, Lusher M, Storey CC & Clarke IN (1997) Plasmid diversity in Chlamydia. Microbiology 143: 1847-1854.
20. Wang Y, Kahane S, Cutcliffe LT, Skilton RJ, Lambden PR & Clarke IN (2011) Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector. PLoS Pathog 7: e1002258.
21. Ward ME (1983) Chlamydial classification, development and structure. Br Med Bull 39: 109-115.
22. Wilson DP, Timms P, McElwain DL & Bavoil PM (2006) Type III secretion, contact-dependent model for the intracellular development of chlamydia. Bull Math Biol 68: 161-178.
23. Wilson DP, Whittum-Hudson JA, Timms P & Bavoil PM (2009) Kinematics of intracellular Chlamydiae provide evidence for contact-dependent development. J Bacteriol 191: 5734-5742.