The generation of transgenic mice with Fat1 and Fad2 genes that have their own polyunsaturated fatty acid biosynthetic pathway

Cellular Physiology and Biochemistry

Volumn 32, Issue 3, 2013, Pages 523-532

  Chen, Yue ^a   Mei, Mei ^a   Zhang, Peng ^a   Ma, Kuiying ^a   Song, Guangqi ^a   Ma, Xin ^b   Zhao, Tianchuang ^a   Tang, Bo ^a   Ouyang, Hongsheng ^a   Li, Guangpeng ^c   Li, Ziyi ^a  

^a JILIN UNIVERSITY   (China)

^b JILIN AGRICULTURAL UNIVERSITY   (China)

^c INNER MONGOLIA UNIVERSITY   (China)

Author keywords

Fad 2; Fat 1; Omega 3 PUFAs; Omega 6 PUFAs; Polyunsaturated fatty acids (PUFAs); Transgenic mice

Indexed keywords

OMEGA 3 FATTY ACID; OMEGA 6 FATTY ACID; POLYUNSATURATED FATTY ACID;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CHROMOSOME 2; CHROMOSOME 3; CHROMOSOME 7; COMPARATIVE STUDY; CONTROLLED STUDY; FAD2 GENE; FAT1 GENE; FATTY ACID ANALYSIS; FEMALE; GAS CHROMATOGRAPHY; GENE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RESTRICTION SITE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EMBRYOPHYTA; MAMMALIA; MUS; MUS MUSCULUS;

ANIMALS; FATTY ACID DESATURASES; FATTY ACIDS, OMEGA-3; FATTY ACIDS, OMEGA-6; FATTY ACIDS, UNSATURATED; FEMALE; LIVER; MALE; MICE; MICE, INBRED C57BL; MICE, INBRED ICR; MICE, TRANSGENIC; ZYGOTE;

EID: 84885010238     PISSN: 10158987     EISSN: 14219778     Source Type: Journal    
DOI: 10.1159/000354456     Document Type: Article

References (36)

1. Damude HG, Zhang H, Farrall L, Ripp KG, Tomb JF, Hollerbach D, Yadav NS: Identification of bifunctional delta12/omega3 fatty acid desaturases for improving the ratio of omega3 to omega6 fatty acids in microbes and plants. Proc Natl Acad Sci USA 2006;103:9446-9451.
2. Satouchi K, Hirano K, Sakaguchi M, Takehara H, Matsuura F: Phospholipids from the free-living nematode caenorhabditis elegans. Lipids 1993;28:837-840.
3. Spychalla JP, Kinney AJ, Browse J: Identification of an animal omega-3 fatty acid desaturase by heterologous expression in arabidopsis. Proc Natl Acad Sci USA 1997;94:1142-1147.
4. Watts JL, Phillips E, Griffing KR, Browse J: Deficiencies in c20 polyunsaturated fatty acids cause behavioral and developmental defects in caenorhabditis elegans fat-3 mutants. Genetics 2003;163:581-589.
5. Gvozdjáková A, Pella D, Kucharská J, Otsuka K, Singh RB: Omega-3-pufa, omega-6-pufa and mitochondria mitochondrial medicine; in Gvozdjáková A (ed). Springer Netherlands, 2008, pp 343-356.
6. Goodnight SJ, Harris W, Connor W: The effects of dietary omega 3 fatty acids on platelet composition and function in man: A prospective, controlled study. Blood 1981;58:880-885.
7. Jump DB: The biochemistry of n-3 polyunsaturated fatty acids. J Biol Chem 2002;277:8755-8758.
8. Riediger ND, Othman RA, Suh M, Moghadasian MH: A systemic review of the roles of n-3 fatty acids in health and disease. J Am Diet Assoc 2009;109:668-679.
9. Watts JL, Browse J: Genetic dissection of polyunsaturated fatty acid synthesis in caenorhabditis elegans. Proc Natl Acad Sci USA 2002;99:5854-5859.
10. Saeki K, Matsumoto K, Kinoshita M, Suzuki I, Tasaka Y, Kano K, Taguchi Y, Mikami K, Hirabayashi M, Kashiwazaki N, Hosoi Y, Murata N, Iritani A: Functional expression of a delta12 fatty acid desaturase gene from spinach in transgenic pigs. Proc Natl Acad Sci USA 2004;101:6361-6366.
11. Kang JX, Wang J, Wu L, Kang ZB: Transgenic mice: Fat-1 mice convert n-6 to n-3 fatty acids. Nature 2004;427:504-504.
12. Kao BT, Lewis KA, DePeters EJ, Van Eenennaam AL: Endogenous production and elevated levels of longchain n-3 fatty acids in the milk of transgenic mice. J Dairy Sci 2006;89:3195-3201.
13. Bongiovanni KD, Depeters EJ, Van Eenennaam AL: Neonatal growth rate and development of mice raised on milk transgenically enriched with omega-3 fatty acids. Pediatr Res 2007;62:412-416.
14. Lai L, Kang JX, Li R, Wang J, Witt WT, Yong HY, Hao Y, Wax DM, Murphy CN, Rieke A, Samuel M, Linville ML, Korte SW, Evans RW, Starzl TE, Prather RS, Dai Y: Generation of cloned transgenic pigs rich in omega-3 fatty acids. Nat Biotechnol 2006;24:435-436.
15. Wu X, Ouyang H, Duan B, Pang D, Zhang L, Yuan T, Xue L, Ni D, Cheng L, Dong S, Wei Z, Li L, Yu M, Sun QY, Chen DY, Lai L, Dai Y, Li GP: Production of cloned transgenic cow expressing omega-3 fatty acids. Transgenic Res 2012;21:537-543.
16. Morimoto KC, Van Eenennaam AL, DePeters EJ, Medrano JF: Endogenous production of n-3 and n-6 fatty acids in mammalian cells. J Dairy Sci 2005;88:1142-1146.
17. Tao F, Zhu SW, Fan J, Cheng BJ: Cloning and sequence analysis of maize fad2 gene. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 2006;32:649-656.
18. Indo Y, Tatemizo A, Abe Y, Suzuki I, Matsumoto K, Hosoi Y, Kinoshita M, Mikami K, Murata N, Iritani A, Saeki K: Functional expression of a humanized gene for an omega-3 fatty acid desaturase from scarlet flax in transfected bovine adipocytes and bovine embryos cloned from the cells. Biochim Biophys Acta 2009;1791:183-190.
19. Kang JX, Wang J: A simplified method for analysis of polyunsaturated fatty acids. BMC Biochem 2005;6:5.
20. Pillai M, Venkataraman G, Kosak S, Torok-Storb B: Integration site analysis in transgenic mice by thermal asymmetric interlaced (tail)-pcr: Segregating multiple-integrant founder lines and determining zygosity. Transgenic Res 2008;17:749-754.
21. Park SH, Kim JN, Park TS, Lee SD, Kim TH, Han BK, Han JY: Cpg methylation modulates tissue-specific expression of a transgene in chickens. Theriogenology 2010;74:805-816.e801.
22. Simopoulos AP: The omega-6/omega-3 fatty acid ratio, genetic variation, and cardiovascular disease. Asia Pac J Clin Nutr 2008;17:S131-134.
23. Madsen L, Pedersen LM, Liaset B, Ma T, Petersen RK, van den Berg S, Pan J, Müller-Decker K, Dülsner ED, Kleemann R, Kooistra T, Døskeland SO, Kristiansen K: Camp-dependent signaling regulates the adipogenic effect of n-6 polyunsaturated fatty acids. J Biol Chem 2008;283:7196-7205.
24. Kang ZB, Ge Y, Chen Z, Cluette-Brown J, Laposata M, Leaf A, Kang JX: Adenoviral gene transfer of caenorhabditis elegans n-3 fatty acid desaturase optimizes fatty acid composition in mammalian cells. Proc Natl Acad Sci USA 2001;98:4050-4054.
25. Chen Q, Liu Q, Wu Z, Wang Z, Gou K: Generation of fad2 transgenic mice that produce omega-6 fatty acids. Sci China C Life Sci 2009;52:1048-1054.
26. Almendingen K, Höstmark AT, Fausa O, Mosdöl A, Aabakken L, Vatn MH: Familial adenomatous polyposis patients have high levels of arachidonic acid and docosahexaenoic acid and low levels of linoleic acid and α-linolenic acid in serum phospholipids. Int J Cancer 2007;120:632-637.
27. Chang ET, Bälter KM, Torrang A, Smedby KE, Melbye M, Sundström C, Glimelius B, Adami H-O: Nutrient intake and risk of non-hodgkin's lymphoma. Am J Epidemiol 2006;164:1222-1232.
28. Reyes N, Reyes I, Tiwari R, Geliebter J: Effect of linoleic acid on proliferation and gene expression in the breast cancer cell line t47d. Cancer Lett 2004;209:25-35.
29. Kilian M, Mautsch I, Gregor JI, Stahlknecht P, Jacobi CA, Schimke I, Guski H, Wenger FA: Influence of conjugated vs. Conventional linoleic acid on liver metastasis and hepatic lipidperoxidation in bop-induced pancreatic cancer in syrian hamster. Prostaglandins Leukot Essent Fatty Acids 2002;67:223-228.
30. Zhu G, Chen H, Wu X, Zhou Y, Lu J, Deng J: A modified n-3 fatty acid desaturase gene from caenorhabditis briggsae produced high proportion of dha and dpa in transgenic mice. Transgenic Res 2008;17:717-725.
31. Jia Q, Lupton JR, Smith R, Weeks BR, Callaway E, Davidson LA, Kim W, Fan Y-Y, Yang P, Newman RA, Kang JX, McMurray DN, Chapkin RS: Reduced colitis-associated colon cancer in fat-1 (n-3 fatty acid desaturase) transgenic mice. Cancer Res 2008;68:3985-3991.
32. Nowak J, Weylandt KH, Habbel P, Wang J, Dignass A, Glickman JN, Kang JX: Colitis-associated colon tumorigenesis is suppressed in transgenic mice rich in endogenous n-3 fatty acids. Carcinogenesis 2007;28:1991-1995.
33. Hudert CA, Weylandt KH, Lu Y, Wang J, Hong S, Dignass A, Serhan CN, Kang JX: Transgenic mice rich in endogenous omega-3 fatty acids are protected from colitis. Proc Natl Acad Sci USA 2006;103:11276-11281.
34. Xia S, Lu Y, Wang J, He C, Hong S, Serhan CN, Kang JX: Melanoma growth is reduced in fat-1 transgenic mice: Impact of omega-6/omega-3 essential fatty acids. Proc Natl Acad Sci USA 2006;103:12499-12504.
35. Connor KM, SanGiovanni JP, Lofqvist C, Aderman CM, Chen J, Higuchi A, Hong S, Pravda EA, Majchrzak S, Carper D, Hellstrom A, Kang JX, Chew EY, Salem N, Serhan CN, Smith LEH: Increased dietary intake of [omega]-3-polyunsaturated fatty acids reduces pathological retinal angiogenesis. Nat Med 2007;13:868-873.
36. Marszalek JR, Lodish HF: Docosahexaenoic acid, fatty acid-interacting proteins, and neuronal function: Breastmilk and fish are good for you. Annu Rev Cell Dev Biol 2005;21:633-657.