Cloning, expression and purification of penicillin binding protein2a (PBP2a) from methicillin resistant staphylococcus aureus: A study on immunoreactivity in Balb/C mouse

Avicenna Journal of Medical Biotechnology

Volumn 5, Issue 4, 2013, Pages 204-211

  Haghighat, Setareh ^a   Siadat, Seyed Davar ^b   Sorkhabadi, Seyed Mehdi Rezayat ^c   Sepahi, Abbas Akhavan ^d   Mahdavi, Mehdi ^b  

^a ISLAMIC AZAD UNIVERSITY   (Iran)

^b PASTEUR INSTITUTE OF IRAN   (Iran)

^c TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^d ISLAMIC AZAD UNIVERSITY   (Iran)

Author keywords

Methicillin resistant Staphylococcus aureus; PBP2a; Recombinant vaccine

Indexed keywords

PENICILLIN BINDING PROTEIN 2A;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE; HUMORAL IMMUNITY; IMMUNIZATION; IMMUNOGENICITY; IMMUNOREACTIVITY; MECA GENE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING;

EID: 84884954470     PISSN: 20082835     EISSN: 20084625     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Kuklin NA, Clark DJ, Secore S, Cook J, Cope LD, McNeely T, et al. A novel Staphylococcus aureus vaccine: iron surface determinant B induces rapid antibody responses in rhesus macaques and specific increased survival in a murine S. aureus sepsis model. Infect Immun 2006; 74(4): 2215-2223.
2. Selvey LA, Whitby M, Johnson B. Nosocomial methicillin resistant Staphylococcus aureus bacteremia: is it any worse than nosocomical methicillin sensitive Staphylococcus aureus bacteremia. Infect Control Hosp Epidemiol 2000; 21(10): 645-648.
3. Cooper BS. Infection control in healthcare settings. parliamentary office of science and technology 2005; 247: 1-5.
4. Lowy FD. Staphylococcus aureus infection. N Engl J Med 1998; 339(8): 520-532.
5. Deleo RF, Otto M, Kreiswirth BN, Chambers HF. Community-associated methicillin-resistant Staphylococcus aureus. Lancet 2010; 375(9725): 1557-1568.
6. Ohwada A, Sekiya M, Hanaki H, Arai KK, Nagaoka I, Hori S, et al. DNA vaccination by mecA sequence evokes an antibacterial immune response against methicillin resistant Staphylococcus aureus. J Antimicrob Chemother 1999; 44(6): 767-774.
7. Roth DM, Senna JP, Machado DC. Evaluation of the humoral immune response in BALB/c mice immunized with a naked DNA vaccine anti-methicillin-resistant Staphylococcus aureus. Genet Mol Res 2006; 5(3): 503-512.
8. Senna JP, Roth DM, Oliveira JS, Machado DC, Santos DS. Protective immune response against methicillin resistant Staphylococcus aureus in a murine model using a DNA vaccine approach. Vaccine 2003; 21(19-20): 2661-2666.
9. Goffin C, Ghuysen JM. Multimodular penicillinbinding proteins: an enigmatic family of orthologs and paralogs. Microbiol Mol Biol Rev 1998; 62(4): 1079-1093.
10. Katayama Y, Ito T, Hiramatsu K. A new class of genetic element, Staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 2000; 44(6): 1549-1555.
11. Pearson H. "Superbug" hurdles key drug barrier. Nature 2002; 418(6897): 469.
12. Chambers HF. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin Microbiol Rev 1997; 10(4): 781-791.
13. Stranger-Jones YK, Bae T, Schneewind O. Vaccine assembly from surface proteins of Staphylococcus aureus. Proc Natl Acad Sci USA 2006; 103(45): 16942-16947.
14. Cohen ML. Staphylococcus aureus: biology, mechanism of virulence, epidemiology. J Pediatr 1986; 108(5 Pt 2): 796-799.
15. Foster TJ. Potential for vaccination against infection caused by Staphylococcus aureus. Vaccine 1991; 9(4): 221-227.
16. Michie CA. Staphylococcal vaccines. Trends Immunol 2002; 23(9): 461-464.
17. Pourmand MR, Foster SJ. A novel bioinformatics approach for Staphylococcal vaccine development. Tehran Univ Med J 2006; 19-27.
18. Weichhart T, Horky M, Söllner J, Gangl S, Henics T, Nagy E, et al. Functional selection of vaccine candidate peptides from Staphylococcus aureus whole-genome expression libraries in vitro. Infect Immun 2003; 71(8): 4633-4641.
19. Etz H, Minh DB, Henics T, Dryla A, Winkler B, Triska C, et al. Identification of in vivo expressed vaccine candidate antigens from Staphylococcus aureus. Proc Natl Acad Sci USA 2002; 99(10): 6573-6578.
20. Jones C. Revised structures for the capsular polysaccharides from Staphylococcus aureus Types 5 and 8, components of novel glycoconjugate vaccines. Carbohydrate Res 2005; 340(6): 1097-1106.
21. Josefsson E, Hartford O, O'Brien L, Patti JM, Foster T. Protection against experimental Staphylococcus aureus arthritis by vaccination with clumping factor A, a novel virulence determinant. J Infect Dis 2001; 184(12): 1572-1580.
22. Ito T, Hiramatsu K, Tomasz A, de Lencastre H, Perreten V, Holden MT, et al. Guidelines for reporting novel mecA gene homologues. Antimicrob Agents Chemother 2012; 56(10): 4997-4999.
23. Mamo W, Jonsson P, Flock JI, Lindberg M, Müller HP, Wadström T, et al. Vaccination against Staphylococcus aureus mastitis: Immunological response of mice vaccinated with fibronectin-binding protein to challenge with S. aureus. Vaccine 1994; 12(11): 988-992.
24. Lim D, Strynadka CJ. Structural basis for the ßlactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus. Nat Struct Biol 2002; 9 (11): 870-876.
25. Hong N, Yun-ying W, Wei-xian CH. Clone and construction of prokaryotic expression plasmid of MRSA-PBP2a. Chinese J Microbiol 2011; 05: 007.
26. Goudarzi G, Sattari M, Roudkenar MH, Montajabi-Niyat M, Zavaran-Hosseini A, Mosavi-Hosseini K. Cloning, expression, purification, and characterization of recombinant flagellin isolated from Pseudomonas aueroginosa. Biotechnol Lett 2009; 31(9): 1353-1360.
27. Tsumoto K, Abe R, Ejima D, Arakawa T. Non denaturing solubilization of inclusion bodies. Curr Pharm Biotechnol 2010; 11(3): 309-312.
28. Casadevall A. Antibody-mediated immunity against intracellular pathogens: two-dimensional thinking comes full circle. Infect Immun 2003; 71(8): 4225-4228.
29. Abbas AK, Litchtman AH, Pillai SH. Cellular and molecular immunology. 6th ed. Philadelphia: Elsevier; 2007.