Cloning, expression, purification, and characterization of recombinant flagellin isolated from Pseudomonas aeruginosa

Biotechnology Letters

Volumn 31, Issue 9, 2009, Pages 1353-1360

  Goudarzi, Gholamreza ^a   Sattari, Morteza ^a   Roudkenar, Mehryar Habibi ^b   Montajabi Niyat, Mehran ^a   Zavaran Hosseini, Ahmad ^a   Mosavi Hosseini, Kamran ^b  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b IRANIAN BLOOD TRANSFUSION ORGANIZATION   (Iran)

Author keywords

fliC; Inclusion bodies; Pseudomonas aeruginosa; Recombinant flagellin

Indexed keywords

CELL MOTILITY; EXPRESSION VECTORS; FLIC; GUANIDINE HYDROCHLORIDE; IN-VITRO; INCLUSION BODIES; P.AERUGINOSA; POLYCLONAL ANTISERUM; PSEUDOMONAS AERUGINOSA; RECOMBINANT FLAGELLIN;

BIOCHEMISTRY; CELL MEMBRANES; GEL PERMEATION CHROMATOGRAPHY; PURIFICATION; RESINS;

CLONING;

BACTERIA (MICROORGANISMS); ORYCTOLAGUS CUNICULUS; PSEUDOMONAS AERUGINOSA;

BACTERIUM ANTIBODY; FLAGELLIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BLOOD; CHEMISTRY; GENE EXPRESSION; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; LAGOMORPH; LOCOMOTION; MOLECULAR CLONING; MOLECULAR WEIGHT; PSEUDOMONAS AERUGINOSA;

ANIMALS; ANTIBODIES, BACTERIAL; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; FLAGELLIN; GENE EXPRESSION; LAGOMORPHA; LOCOMOTION; MOLECULAR WEIGHT; PSEUDOMONAS AERUGINOSA;

EID: 70349575018     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0026-1     Document Type: Article

References (23)

1. Allison JS, Dawson M, Montie TC et al (1985) Electrophoretic separation and molecular weight characterization of Pseudomonas aeruginosa H-antigen flagellins. Infect Immun 49:770-774
2. Ansorg R (1978) Flagella specific H antigenic schema of Pseudomonas aeruginosa. Zentbl. Bakteriol. Parasitenkd. Infektkrankh Hyg Abt 1(224):228-238
3. Arora SK, Wolfgang MC, Ramphal R et al (2004) Sequence polymorphism in the glycosylation island and flagellins of Pseudomonas aeruginosa. J Bacteriol 186(7):2115-2122
4. Arora SK, Neely AN, Ramphal R et al (2005) Role of motility and flagellin glycosylation in the pathogenesis of Pseudomonas aeruginosa burn wound infections. Infect Immun 73(7):4395-4398
5. Brett PJ, Mah DCW, Woods DE (1994) Isolation and characterization of Pseudomonas pseudomallei flagellin proteins. Infect Immun 62(5):1914-1919
6. Brimer CD, Montie TC (1998) Cloning and comparison of fliC genes and identification of glycosylation in the flagellin of Pseudomonas aeruginosa a-type strains. J Bacteriol 180(12):3209-3217
7. D̈oring G, Pier GB (2008) Vaccines and immunotherapy against Pseudomonas aeruginosa. Vaccine 26:1011-1024
8. Döring G, Meisner C, Stern M (2007) A double-blind randomized placebo-controlled phase III study of a Pseudomonas aeruginosa flagella vaccine in cystic fibrosis patients. Proc Natl Acad Sci USA 26(104):11020-11025
9. Driscoll JA, Brody SL, Kollef MH (2007) The epidemiology, pathogenesis and treatment of Pseudomonas aeruginosa infections. Drugs 67:351-368
10. Feldman M, Bryan R, Prince A et al (1998) Role of flagella in pathogenesis of Pseudomonas aeruginosa pulmonary infection. Infect Immun 66(1):43-51
11. Holder IA, Naglich JG (1986) Experimental studies of the pathogenesis of infection of Pseudomonas aeruginosa: immunization using divalent flagella preparations. J Trauma 26(2):118-121
12. Ibrahim GF, Fleet GH, Walker RA (1985) Method for the isolation of highly purified Salmonella flagellins. J Clin Microbiol 22(6):1040-1044
13. Kelly-Wintenberg K, Montie TC (1989) Cloning and expression of Pseudomonas aeruginosa flagellin in Escherichia coli. J Bacteriol 171(11):6357-6362
14. Montie TC, Holder IA (1982) Loss of virulence associated with absence of flagellum in an isogenic mutant of Pseudomonas aeruginosa in the burned-mouse model. Infect Immun 38(3):1296-1298
15. Montie TC, Craven RC, Holder IA (1982) Flagellar preparations from Pseudomonas aeruginosa: isolation and characterization. Infect Immun 35(1):281-288
16. Namba K, Yamashita I, Vonderviszt F (1989) Structure of the core and central channel of bacterial flagella. Nature 342:648-654
17. Ramos HC, Rumbo M, Sirard JC (2004) Bacterial flagellins: mediators of pathogenicity and host immune responses in mucosa. Trends Microbiol 12(11):509-517
18. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
19. Spangenberg C, Heuer T, Tummler B et al (1996) Genetic diversity of flagellins of Pseudomonas aeruginosa. FEBS Lett 396:213-217
20. Steiner TS (2007) How flagellin and toll-Like receptor 5 contribute to enteric infection. Infect Immun 75(2):545-552
21. Tseng J, Do J, Machen TE et al (2006) Innate immune responses of human tracheal epithelium to Pseudomonas aeruginosa flagellin, TNF-α and IL-1β. Am J Physiol Cell Physiol 290:678-690
22. Wilson DR, Beveridge TJ (1993) Bacterial flagellar filaments and their component flagellins. Can J Microbiol 39:451-472
23. Winstanley C, Coulson MA, Wepner B et al (1996) Flagellin gene and protein variation amongst clinical isolates of Pseudomonas aeruginosa. Microbiology 142:2145-2151