Analysis of lipid droplets in hepatocytes

Methods in Cell Biology

Volumn 116, Issue , 2013, Pages 107-127

  Wang, Huajin ^a   Quiroga, Ariel D ^b   Lehner, Richard ^c  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

^c UNIVERSITY OF ALBERTA   (Canada)

Author keywords

BODIPY fatty acids; Cytosolic lipid droplets; Fluorescence microscopy; Liver; Lumenal lipid droplets; Subcellular fractionation

Indexed keywords

TRIACYLGLYCEROL; VERY LOW DENSITY LIPOPROTEIN;

ANIMAL; ARTICLE; BODIPY FATTY ACIDS; CELL FRACTIONATION; CELL INCLUSION; CHEMISTRY; CYTOLOGY; CYTOSOL; CYTOSOLIC LIPID DROPLETS; ENDOPLASMIC RETICULUM; FLUORESCENCE MICROSCOPY; HUMAN; ISOLATION AND PURIFICATION; LIVER; LIVER CELL; LUMENAL LIPID DROPLETS; METABOLISM;

BODIPY FATTY ACIDS; CYTOSOLIC LIPID DROPLETS; FLUORESCENCE MICROSCOPY; LIVER; LUMENAL LIPID DROPLETS; SUBCELLULAR FRACTIONATION;

ANIMALS; CYTOSOL; ENDOPLASMIC RETICULUM; HEPATOCYTES; HUMANS; INCLUSION BODIES; LIPOPROTEINS, VLDL; LIVER; MICROSCOPY, FLUORESCENCE; TRIGLYCERIDES;

EID: 84884937794     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-408051-5.00007-3     Document Type: Chapter

References (27)

1. Alexander C.A., Hamilton R.L., Havel R.J. Subcellular localization of B apoprotein of plasma lipoproteins in rat liver. The Journal of Cell Biology 1976, 69:241-263.
2. Bickel P.E., Tansey J.T., Welte M.A. PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores. Biochimica et Biophysica Acta 2009, 1791:419-440.
3. Brasaemle D.L., Wolins N.E. Isolation of lipid droplets from cells by density gradient centrifugation. Current Protocols in Cell Biology 2006, Chapter 3, Unit 3.15.
4. Chattopadhyay A. Chemistry and biology of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled lipids: Fluorescent probes of biological and model membranes. Chemistry and Physics of Lipids 1990, 53:1-15.
5. DiDonato D., Brasaemle D.L. Fixation methods for the study of lipid droplets by immunofluorescence microscopy. Journal of Histochemistry and Cytochemistry 2003, 51:773-780.
6. Farese R.V., Walther T.C. Lipid droplets finally get a little R-E-S-P-E-C-T. Cell 2009, 139:855-860.
7. Fujimoto Y., Itabe H., Sakai J., Makita M., Noda J., Mori M., et al. Identification of major proteins in the lipid droplet-enriched fraction isolated from the human hepatocyte cell line HuH7. Biochimica et Biophysica Acta 2004, 1644:47-59.
8. Hamilton R.L., Wong J.S., Cham C.M., Nielsen L.B., Young S.G. Chylomicron-sized lipid particles are formed in the setting of apolipoprotein B deficiency. Journal of Lipid Research 1998, 39:1543-1557.
9. Krahmer N., Hilger M., Kory N., Wilfling F., Stoehr G., Mann M., et al. Protein correlation profiles identify lipid droplet proteins with high confidence. Molecular & Cellular Proteomics 2013, 12:1115-1126.
10. Kuerschner L., Ejsing C.S., Ekroos K., Shevchenko A., Anderson K.I., Thiele C. Polyene-lipids: A new tool to image lipids. Nature Methods 2005, 2:39-45.
11. Kuhnlein R.P. Thematic review series: Lipid droplet synthesis and metabolism: from yeast to man. Lipid droplet-based storage fat metabolism in Drosophila. Journal of Lipid Research 2012, 53:1430-1436.
12. Lehner R., Kuksis A. Triacylglycerol synthesis by an sn-1,2(2,3)-diacylglycerol transacylase from rat intestinal microsomes. Journal of Biological Chemistry 1993, 268:8781-8786.
13. Ohsaki Y., Maeda T., Fujimoto T. Fixation and permeabilization protocol is critical for the immunolabeling of lipid droplet proteins. Histochemistry and Cell Biology 2005, 124:445-452.
14. Olofsson S.O., Bostrom P., Andersson L., Rutberg M., Perman J., Boren J. Lipid droplets as dynamic organelles connecting storage and efflux of lipids. Biochimica et Biophysica Acta 2009, 1791:448-458.
15. Ong K.T., Mashek M.T., Bu S.Y., Greenberg A.S., Mashek D.G. Adipose triglyceride lipase is a major hepatic lipase that regulates triacylglycerol turnover and fatty acid signaling and partitioning. Hepatology 2011, 53:116-126.
16. Ontko J.A., Perrin L.W., Horne L.S. Isolation of hepatocellular lipid droplets: The separation of distinct subpopulations. Journal of Lipid Research 1986, 27:1097-1103.
17. Pagano R.E., Martin O.C., Kang H.C., Haugland R.P. A novel fluorescent ceramide analogue for studying membrane traffic in animal cells: Accumulation at the Golgi apparatus results in altered spectral properties of the sphingolipid precursor. The Journal of Cell Biology 1991, 113:1267-1279.
18. Quiroga A.D., Li L., Trotzmuller M., Nelson R., Proctor S.D., Kofeler H., et al. Deficiency of carboxylesterase 1/esterase-x results in obesity, hepatic steatosis, and hyperlipidemia. Hepatology 2012, 56:2188-2198.
19. Sundaram M., Zhong S., Bou Khalil M., Zhou H., Jiang Z.G., Zhao Y., et al. Functional analysis of the missense APOC3 mutation Ala23Thr associated with human hypotriglyceridemia. Journal of Lipid Research 2010, 51:1524-1534.
20. Thumser A.E., Storch J. Characterization of a BODIPY-labeled fluorescent fatty acid analogue. Binding to fatty acid-binding proteins, intracellular localization, and metabolism. Molecular and Cellular Biochemistry 2007, 299:67-73.
21. Turro S., Ingelmo-Torres M., Estanyol J.M., Tebar F., Fernandez M.A., Albor C.V., et al. Identification and characterization of associated with lipid droplet protein 1: A novel membrane-associated protein that resides on hepatic lipid droplets. Traffic 2006, 7:1254-1269.
22. Walther T.C., Farese R.V. Lipid droplets and cellular lipid metabolism. Annual Review of Biochemistry 2012, 81:687-714.
23. Wang H., Gilham D., Lehner R. Proteomic and lipid characterization of apolipoprotein B-free luminal lipid droplets from mouse liver microsomes: Implications for very low density lipoprotein assembly. Journal of Biological Chemistry 2007, 282:33218-33226.
24. Wang H., Wei E., Quiroga A.D., Sun X., Touret N., Lehner R. Altered lipid droplet dynamics in hepatocytes lacking triacylglycerol hydrolase expression. Molecular Biology of the Cell 2010, 21:1991-2000.
25. Yang L., Ding Y., Chen Y., Zhang S., Huo C., Wang Y., et al. The proteomics of lipid droplets: Structure, dynamics, and functions of the organelle conserved from bacteria to humans. Journal of Lipid Research 2012, 53:1245-1253.
26. Yao Z.M., Vance D.E. The active synthesis of phosphatidylcholine is required for very low density lipoprotein secretion from rat hepatocytes. Journal of Biological Chemistry 1988, 263:2998-3004.
27. Yao Z., Zhou H., Figeys D., Wang Y., Sundaram M. Microsome-associated lumenal lipid droplets in the regulation of lipoprotein secretion. Current Opinion in Lipidology 2013, 24:160-170.