Continuous enzyme-coupled assay for microbial transglutaminase activity

Analytical Biochemistry

Volumn 441, Issue 2, 2013, Pages 169-173

  Oteng Pabi, Samuel K ^a   Keillor, Jeffrey W ^a  

^a UNIVERSITY OF OTTAWA   (Canada)

Author keywords

Enzyme coupled assay; Glutamate dehydrogenase; Hydroxamate assay; Microbial transglutaminase; Microtiter plate; Transamidation

Indexed keywords

AMINES; AMINO ACIDS; CATALYSIS; ENZYMES; PEPTIDES;

GLUTAMATE DEHYDROGENASE; HYDROXAMATE; MICROBIAL TRANSGLUTAMINASE; MICROTITER PLATES; TRANSAMIDATION;

PLANTS (BOTANY);

2 OXOGLUTARIC ACID; GLUTAMATE DEHYDROGENASE; GLUTAMINE; HYDROXAMIC ACID; MICROBIAL ENZYME; PEPTIDE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; BINDING AFFINITY; BIOCATALYSIS; CONJUGATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME SUBSTRATE; LIMIT OF DETECTION; PRIORITY JOURNAL; PROTEIN BINDING;

BACTERIA (MICROORGANISMS);

ENZYME-COUPLED ASSAY; GLUTAMATE DEHYDROGENASE; HYDROXAMATE ASSAY; MICROBIAL TRANSGLUTAMINASE; MICROTITER PLATE; TRANSAMIDATION;

ENZYME ASSAYS; HYDROXAMIC ACIDS; LIMIT OF DETECTION; PEPTIDES; STREPTOMYCES; SUBSTRATE SPECIFICITY; TRANSGLUTAMINASES;

EID: 84884927476     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.07.014     Document Type: Article

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