Live-Cell Fluorescent Speckle Microscopy of Actin Cytoskeletal Dynamics and Their Perturbation by Drug Perfusion

Cell Biology, Four-Volume Set

Volumn 3, Issue , 2006, Pages 137-151

  Gupton, Stephanie L ^a   Waterman Storer, Clare M ^a  

^a NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84884892009     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-012164730-8/50142-8     Document Type: Chapter

References (28)

1. Adams M.C., Matov A., Yarar D., Gupton S.L., Danuser G., Waterman-Storer C.M. Siginal analysis of total internal reflection fluorescent speckle microscopy (TIR-FSM) and widefield epi-fluorescence FSM of the actin cytoskeleton and focal adhesions in living cells. J Microsc. 2004, 216:138-152.
2. Adams M.C., Salmon W.C., Gupton S.L., Cohan C.S., Wittmann T., Prigozhina N., Waterman-Storer C.M. A high-speed multispectral spinning-disk confocal microscope system for fluorescent speckle microscopy of living cells. Methods. 2003, 29(1):29-41.
3. Bulinski J.C., Odde D.J., Howell B.J., Salmon T.D., Waterman-Storer C.M. Rapid dynamics of the microtubule binding of ensconsin in vivo. J. Cell Sci. 2001, 114(Pt 21):3885-3897.
4. Desai A., Mitchison T.J. Microtubule polymerization dynamics. Annu. Rev. Cell Dev. Biol. 1997, 13:83-117.
5. Grego S., Cantillana V., Salmon E.D. Microtubule treadmilling in vitro investigated by fluorescence speckle and confocal microscopy. Biophys. J. 2001, 81(1):66-78.
6. Gupton S.L., Salmon W.C., Waterman-Storer C.M. Converging populations of f-actin promote breakage of associated microtubules to spatially regulate microtubule turnover in migrating cells. Curr. Biol. 2002, 12(22):1891-1899.
7. Inoué S., Spring K.R. Video Microscopy 1997, Plenum Press, New York.
8. Maddox P., Desai A., Oegema K., Mitchison T.J., Salmon E.D. Poleward microtubule flux is a major component of spindle dynamics and anaphase in mitotic Drosophila embryos. Curr. Biol. 2002, 12(19):1670-1674.
9. Maddox P., Straight A., Coughlin P., Mitchison T.J., Salmon E.D. Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: Implications for spindle mechanics. J. Cell Biol. 2003, 162(3):377-382.
10. Maddox P.S., Moree B., Canman J.C., Salmon E.D. Spinning disk confocal microscope system for rapid high-resolution, multimode, fluorescence speckle microscopy and green fluorescent protein imaging in living cells. Methods Enzymol. 2003, 360:597-617.
11. Mikhailov A.V., Gundersen G.G. Centripetal transport of microtubules in motile cells. Cell Motil. Cytoskel. 1995, 32(3):173-186.
12. Pollard T.D., Blanchoin L., Mullins R.D. Molecular mechanisms controlling actin filament dynamics in nonmuscle cells. Annu. Rev. Biophys. Biomol. Struct. 2000, 29:545-576.
13. Ponti A., Vallotton P., Salmon W.C., Waterman-Storer C.M., Danuser G. Computational analysis of f-actin turnover in cortical actin meshworks using fluorescent speckle microscopy. Biophys. J. 2003, 84(5):3336-3352.
14. Prasher D.C. Using GFP to see the light. Trends Genet. 1995, 11(8):320-323.
15. Small J.V. Organization of actin in the leading edge of cultured cells: Influence of osmium tetroxide and dehydration on the ultrastructure of actin meshworks. J. Cell Biol. 1981, 91(3 Pt 1):695-705.
16. Stelzer E.H.K. Contrast, resolution, pixelation, dynamic range and signal-to-noise ratio: Fundamental limits to resolution in fluorescence light microscopy. J. Microsc. 1998, 189:15-24.
17. Svitkina T.M., Verkhovsky A.B., McQuade K.M., Borisy G.G. Analysis of the actin-myosin II system in fish epidermal keratocytes: Mechanism of cell body translocation. J. Cell Biol. 1997, 139(2):397-415.
18. Verkhovsky A.B., Svitkina T.M., Borisy G.G. Self-polarization and directional motility of cytoplasm. Curr. Biol. 1999, 9(1):11-20.
19. Wang Y.L., Lanni F., McNeil P.L., Ware B.R., Taylor D.L. Mobility of cytoplasmic and membrane-associated actin in living cells. Proc. Nalt. Acad. Sci. USA 1982, 79(15):4660-4664.
20. Watanabe N., Mitchison T.J. Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science 2002, 295(5557):1083-1086.
21. Waterman-Storer C.M. Fluorescent speckle microscopy (FSM) of microtubules and actin in living cells. Current Protocols in Cell Biology 2002, Wiley, New York. K.S. Morgan (Ed.).
22. Waterman-Storer C.M. Microtubule/organelle motility assays. Current Protocols in Cell Biology 2002, Wiley, New York. J.S. Bonifacio, M. Dasso, J.B. Harford, J. Lippincott-Schwartz, K.M. Yamada (Eds.).
23. Waterman-Storer C.M., Desai A., Bulinski J.C., Salmon E.D. Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells. Curr. Biol. 1998, 8(22):1227-1230.
24. Waterman-Storer C.M., Salmon E.D. Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling. J. Cell Biol. 1997, 139(2):417-434.
25. Waterman-Storer C.M., Salmon E.D. How microtubules get fluorescent speckles. Biophys. J. 1998, 75(4):2059-2069.
26. Waterman-Storer C.M., Salmon E.D. Fluorescent speckle microscopy of microtubules: How low can you go?. FASEB J. 1999, 2:S225-S230.
27. Waterman-Storer C.M., Salmon W.C., Salmon E.D. Feedback interactions between cell-cell adherens junctions and cytoskeletal dynamics in newt lung epithelial cells. Mol. Biol. Cell 2000, 11(7):2471-2483.
28. Waterman-Storer C.M., Sanger J.W., Sanger J.M. Dynamics of organelles in the mitotic spindles of living cells: membrane and microtubule interactions. Cell Motil. Cytoskel. 1993, 26(1):19-39.