Microtubule treadmilling in vitro investigated by fluorescence speckle and confocal microscopy

Biophysical Journal

Volumn 81, Issue 1, 2001, Pages 66-78

  Grego, Sonia ^a   Cantillana, Viviana ^a   Salmon, E D ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

KINESIN; TUBULIN;

ANIMAL TISSUE; ARTICLE; BRAIN; CONFOCAL MICROSCOPY; DYNAMICS; FLUORESCENCE; IN VITRO STUDY; MICROTUBULE; MICROTUBULE ASSEMBLY; NONHUMAN; STEADY STATE; SWINE; SYSTEM ANALYSIS; TREADMILL;

ANIMALIA; SUIDAE; SUS SCROFA;

EID: 0034949813     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)75680-9     Document Type: Article

References (35)

1. Head-to-tail polymerization of microtubules in vitro
2. Anaphase A chromosome movement and poleward spindle microtubule flux occur at the same rates in Xenopus extract spindles
3. Microtubule polymerization dynamics
4. "Gliding assays" for motor proteins: A theoretical analysis
5. Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends
6. Phase diagram of microtubules
7. Microtubules grow and shorten at intrinsically variable rates
8. Dynamics of microtubules visualized by darkfield microscopy: Treadmilling and dynamic instability
9. Assay of microtubule movement driven by single kinesin molecules
10. Dissociation of the tubulin-sequestering and microtubule catastrophe-promoting activities of oncoprotein 18/Stathmin
11. Force generation by microtubule assembly/disassembly in mitosis and related movements
12. Direct observation of steady-state microtubule dynamics
13. Role of GTP hydrolysis in microtubule treadmilling and assembly
14. Opposite end assembly and disassembly of microtubules at steady state in vitro
15. Microtubule treadmilling: What goes around come around
16. Polewards microtubule flux in the mitotic spindle: Evidence from photoactivation of fluorescence
17. Dynamic instability of microtubule growth
18. Poleward kinetochore fiber movement occurs during both metaphase and anaphase A in newt lung cell mitotis
19. Rapid treadmilling of brain microtubules free of microtubule-associated proteins in vitro and its suppression by tau
20. NEM tubulin inhibits microtubule minus end assembly by a reversible capping mechanism
21. Microtubule treadmilling in vivo
22. Centrosomal control of microtubule dynamics
23. Kinetic stabilization of microtubule dynamic instability in vitro by vinblastine
24. How tubulin subunits are lost from the shortening ends of microtubules
25. A metastable intermediate state of microtubule dynamic instability that differs significantly between plus and minus ends
26. Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro
27. Asymmetric behavior of severed microtubule ends after ultraviolet-microbeam irradiation of individual microtubules in vitro
28. Dynamic instability of individual microtubules analyzed by video light microscopy: Rate constants and transition frequencies
29. Dilution of individual microtubules observed in real time in vitro: Evidence that cap size is small and independent of elongation rate
30. Microtubule dynamics: Treadmilling comes around again
31. Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling
32. How microtubules get fluorescent speckles
33. Oscillating mitotic newt lung cell kinetochores are, on average, under tension and rarely push
34. Head to tail polymerization of actin
35. Kinetochore microtubule dynamics and the metaphase-anaphase transition