-
2
-
-
0039234462
-
Subtilisins: primary structure, chemical and physical properties
-
Academic Press, London
-
Markland F.S., Smith E.L. Subtilisins: primary structure, chemical and physical properties. The Enzymes 1971, vol. 3:561-608. Academic Press, London.
-
(1971)
The Enzymes
, vol.3
, pp. 561-608
-
-
Markland, F.S.1
Smith, E.L.2
-
7
-
-
0030896832
-
Subtilases: The superfamily of subtilisin-like serine proteases
-
Siezen R.J., Leunissen J.A.M. Subtilases: The superfamily of subtilisin-like serine proteases. Protein Sci. 1997, 6:501-523.
-
(1997)
Protein Sci.
, vol.6
, pp. 501-523
-
-
Siezen, R.J.1
Leunissen, J.A.M.2
-
8
-
-
0014682668
-
Structure of subtilisin BPN' at 2.5Å resolution
-
Wright C.S., Alden R.A., Kraut J. Structure of subtilisin BPN' at 2.5Å resolution. Nature 1969, 221:235-242.
-
(1969)
Nature
, vol.221
, pp. 235-242
-
-
Wright, C.S.1
Alden, R.A.2
Kraut, J.3
-
9
-
-
0014201592
-
Three-dimensional structure of tosylα-chymotrypsin
-
Matthews B.W., Sigler P.B., Henderson R., Blow D.M. Three-dimensional structure of tosylα-chymotrypsin. Nature 1967, 214:652-656.
-
(1967)
Nature
, vol.214
, pp. 652-656
-
-
Matthews, B.W.1
Sigler, P.B.2
Henderson, R.3
Blow, D.M.4
-
10
-
-
0015517292
-
An X-ray crystallographic study of the binding of peptide chloromethyl ketone inhibitors to subtilisin BPN'
-
Robertus J.D., Alden R.A., Birktoft J.J., Kraut J., Powers J.C., Wilcox P.E. An X-ray crystallographic study of the binding of peptide chloromethyl ketone inhibitors to subtilisin BPN'. Biochemistry 1972, 11:2439-2449.
-
(1972)
Biochemistry
, vol.11
, pp. 2439-2449
-
-
Robertus, J.D.1
Alden, R.A.2
Birktoft, J.J.3
Kraut, J.4
Powers, J.C.5
Wilcox, P.E.6
-
11
-
-
0017324044
-
Serine proteases: structure and mechanism of catalysis
-
Kraut J. Serine proteases: structure and mechanism of catalysis. Annu. Rev. Biochem. 1977, 46:331-358.
-
(1977)
Annu. Rev. Biochem.
, vol.46
, pp. 331-358
-
-
Kraut, J.1
-
12
-
-
0011188780
-
Structure and function of serine proteases
-
CRC Press, Boca Raton, FL, A. Neuberger, K. Brocklehurst (Eds.)
-
Polgár L. Structure and function of serine proteases. Mechanisms of Protease Action 1987, CRC Press, Boca Raton, FL. A. Neuberger, K. Brocklehurst (Eds.).
-
(1987)
Mechanisms of Protease Action
-
-
Polgár, L.1
-
13
-
-
0001247133
-
A new enzyme containing a synthetically formed active site. Thiol-Subtilisin
-
Polgár L., Bender M.L. A new enzyme containing a synthetically formed active site. Thiol-Subtilisin. J. Am. Chem. Soc. 1966, 88:3153-3154.
-
(1966)
J. Am. Chem. Soc.
, vol.88
, pp. 3153-3154
-
-
Polgár, L.1
Bender, M.L.2
-
14
-
-
0013965360
-
The conversion of serine at the active site of subtilisin to cysteine: A 'chemical mutation'
-
Neet K.E., Koshland D.E. The conversion of serine at the active site of subtilisin to cysteine: A 'chemical mutation'. Proc. Nati Acad. Sci. USA 1966, 56:1606-1611.
-
(1966)
Proc. Nati Acad. Sci. USA
, vol.56
, pp. 1606-1611
-
-
Neet, K.E.1
Koshland, D.E.2
-
15
-
-
0021112782
-
Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis
-
Wells J.A., Ferrari E., Henner D.J., Chen E.Y. Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis. Nucleic Acids Res. 1983, 11:7911-7925.
-
(1983)
Nucleic Acids Res.
, vol.11
, pp. 7911-7925
-
-
Wells, J.A.1
Ferrari, E.2
Henner, D.J.3
Chen, E.Y.4
-
16
-
-
0023783073
-
Subtilisin - an enzyme designed to be engineered
-
Wells J.A., Estell D.A. Subtilisin - an enzyme designed to be engineered. Trends Biochem. Sci. 1988, 13:291-297.
-
(1988)
Trends Biochem. Sci.
, vol.13
, pp. 291-297
-
-
Wells, J.A.1
Estell, D.A.2
-
17
-
-
0024280501
-
Dissecting the catalytic triad of a serine protease
-
Carter P., Wells J. Dissecting the catalytic triad of a serine protease. Nature 1988, 322:546-568.
-
(1988)
Nature
, vol.322
, pp. 546-568
-
-
Carter, P.1
Wells, J.2
-
18
-
-
0018798005
-
Comparison of the kinetic specificity of subtilisin and thiolsubtilisin toward n-alkyl p-nitrobenzyl esters
-
Philipp M., Tsai L.-H., Bender M.L. Comparison of the kinetic specificity of subtilisin and thiolsubtilisin toward n-alkyl p-nitrobenzyl esters. Biochemistry 1979, 18:3769-3773.
-
(1979)
Biochemistry
, vol.18
, pp. 3769-3773
-
-
Philipp, M.1
Tsai, L.-H.2
Bender, M.L.3
-
19
-
-
0023646665
-
Rational modification of enzyme catalysis by engineering surface charge
-
Russell A.J., Fersht A.R. Rational modification of enzyme catalysis by engineering surface charge. Nature 1987, 328:496-500.
-
(1987)
Nature
, vol.328
, pp. 496-500
-
-
Russell, A.J.1
Fersht, A.R.2
-
20
-
-
0025731181
-
Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene
-
van der Laan J.C., Gerritse G., Mulleners L.J.S.M., Van der Hoek R.A.C., Quax W.J. Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene. Appl. Environ. Microbiol. 1991, 57:901-909.
-
(1991)
Appl. Environ. Microbiol.
, vol.57
, pp. 901-909
-
-
van der Laan, J.C.1
Gerritse, G.2
Mulleners, L.J.S.M.3
Van der Hoek, R.A.C.4
Quax, W.J.5
-
21
-
-
0026501813
-
Crystal structure of the alkaline proteinase savinase from Bacillus lentus at 1.4 Å resolution
-
Betzel C., Klupsch S., Papendorf G., Hastrup S., Branner S., Wilson K.S. Crystal structure of the alkaline proteinase savinase from Bacillus lentus at 1.4 Å resolution. J. Mol. Biol. 1992, 223:427-445.
-
(1992)
J. Mol. Biol.
, vol.223
, pp. 427-445
-
-
Betzel, C.1
Klupsch, S.2
Papendorf, G.3
Hastrup, S.4
Branner, S.5
Wilson, K.S.6
-
22
-
-
0022445901
-
Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering
-
Estell D.A., Graycar T.P., Miller J.V., Powers D.B., Burnier J.P., Ng P.G., Wells J.A. Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering. Science 1986, 233:659-663.
-
(1986)
Science
, vol.233
, pp. 659-663
-
-
Estell, D.A.1
Graycar, T.P.2
Miller, J.V.3
Powers, D.B.4
Burnier, J.P.5
Ng, P.G.6
Wells, J.A.7
-
23
-
-
0020688134
-
Kinetics of subtilisin and thiolsubtilisin
-
Philipp M., Bender M.L. Kinetics of subtilisin and thiolsubtilisin. Mol. Cell. Biochem. 1983, 51G:5-32.
-
(1983)
Mol. Cell. Biochem.
, vol.51 G
, pp. 5-32
-
-
Philipp, M.1
Bender, M.L.2
-
24
-
-
0023691688
-
The engineering of binding affinity at metal binding sites for the stabilization of proteins: subtilisin as a test case
-
Pantoliano M.W., Whitlow M., Wood J.F., Rollence M.L., Finzel B.C., Gilliland G.L., Poulos T.L., Bryan P.N. The engineering of binding affinity at metal binding sites for the stabilization of proteins: subtilisin as a test case. Biochemistry 1988, 27:8311-8317.
-
(1988)
Biochemistry
, vol.27
, pp. 8311-8317
-
-
Pantoliano, M.W.1
Whitlow, M.2
Wood, J.F.3
Rollence, M.L.4
Finzel, B.C.5
Gilliland, G.L.6
Poulos, T.L.7
Bryan, P.N.8
-
25
-
-
0024962392
-
Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding
-
Pantoliano M.W., Whitlow M., Wood J.F., Dodd S.W., Hardman K.D., Rollence M.L., Bryan P.N. Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding. Biochemistry 1989, 28:7205-7213.
-
(1989)
Biochemistry
, vol.28
, pp. 7205-7213
-
-
Pantoliano, M.W.1
Whitlow, M.2
Wood, J.F.3
Dodd, S.W.4
Hardman, K.D.5
Rollence, M.L.6
Bryan, P.N.7
-
26
-
-
0025264347
-
Enzymes in organic synthesis: use of subtilisin and a highly stable mutant derived from multiple site-specific mutations
-
Wong C.-H., Chen S.-T., Hennen W.J., Bibbs J.A., Wang Y.-F., Liu J.L.-C., Pantoliano M.W., Whitlow M., Bryan P.N. Enzymes in organic synthesis: use of subtilisin and a highly stable mutant derived from multiple site-specific mutations. J. Am. Chem. Soc. 1990, 112:945-953.
-
(1990)
J. Am. Chem. Soc.
, vol.112
, pp. 945-953
-
-
Wong, C.-H.1
Chen, S.-T.2
Hennen, W.J.3
Bibbs, J.A.4
Wang, Y.-F.5
Liu, J.L.-C.6
Pantoliano, M.W.7
Whitlow, M.8
Bryan, P.N.9
-
27
-
-
0027205210
-
Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide
-
Chen K., Arnold F.H. Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide. Proc. Natl Acad. Sci. USA 1993, 90:5618-5622.
-
(1993)
Proc. Natl Acad. Sci. USA
, vol.90
, pp. 5618-5622
-
-
Chen, K.1
Arnold, F.H.2
-
29
-
-
0029969577
-
Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide
-
You L., Arnold F.H. Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide. Protein Eng. 1996, 9:77-83.
-
(1996)
Protein Eng.
, vol.9
, pp. 77-83
-
-
You, L.1
Arnold, F.H.2
-
30
-
-
0014802965
-
Subtilisin BPN': inactivation by chloromethyl ketone derivatives of peptide substrates
-
Morihara K., Oka T. Subtilisin BPN': inactivation by chloromethyl ketone derivatives of peptide substrates. Arch. Biochem. Biophys. 1970, 138:526-531.
-
(1970)
Arch. Biochem. Biophys.
, vol.138
, pp. 526-531
-
-
Morihara, K.1
Oka, T.2
-
31
-
-
0017253438
-
Polypeptide halomethyl ketones bind to serine proteases as analogs of the tetrahedral intermediate
-
Poulos T.L., Alden R.A., Freer S.T., Birktoft J.J., Kraut J. Polypeptide halomethyl ketones bind to serine proteases as analogs of the tetrahedral intermediate. J. Biol. Chem. 1976, 251:1097-1103.
-
(1976)
J. Biol. Chem.
, vol.251
, pp. 1097-1103
-
-
Poulos, T.L.1
Alden, R.A.2
Freer, S.T.3
Birktoft, J.J.4
Kraut, J.5
-
32
-
-
0016723519
-
X-ray crystallographic study of boronic acid adducts with subtilisin BPN' (Novo)
-
Matthews D.A., Alden R.A., Birktoft J.J., Freer S.T., Kraut J. X-ray crystallographic study of boronic acid adducts with subtilisin BPN' (Novo). J. Biol. Chem. 1975, 250:7120-7126.
-
(1975)
J. Biol. Chem.
, vol.250
, pp. 7120-7126
-
-
Matthews, D.A.1
Alden, R.A.2
Birktoft, J.J.3
Freer, S.T.4
Kraut, J.5
-
33
-
-
0020480240
-
Thermodynamics and kinetics of single residue replacements in avian ovomucoid third domains: effect on inhibitor interactions with serine proteinases
-
Empie M.W., Laskowski M. Thermodynamics and kinetics of single residue replacements in avian ovomucoid third domains: effect on inhibitor interactions with serine proteinases. Biochemistry 1982, 21:2274-2284.
-
(1982)
Biochemistry
, vol.21
, pp. 2274-2284
-
-
Empie, M.W.1
Laskowski, M.2
-
34
-
-
0025884348
-
X-ray structure of the antibiotic bacitracin A
-
Pfeffer S., Hohne W., Branner S., Wilson K., Betzel C. X-ray structure of the antibiotic bacitracin A. FEBS Lett. 1991, 285:115-119.
-
(1991)
FEBS Lett.
, vol.285
, pp. 115-119
-
-
Pfeffer, S.1
Hohne, W.2
Branner, S.3
Wilson, K.4
Betzel, C.5
-
35
-
-
84884827719
-
Eglins
-
Elsevier, Amsterdam, A.J. Barrett, G. Salvesen (Eds.)
-
Seemüller U., Dodt J., Fink E., Fritz H. Eglins. Proteinase Inhibitors 1986, 347-355. Elsevier, Amsterdam. A.J. Barrett, G. Salvesen (Eds.).
-
(1986)
Proteinase Inhibitors
, pp. 347-355
-
-
Seemüller, U.1
Dodt, J.2
Fink, E.3
Fritz, H.4
-
36
-
-
0018873523
-
Protein inhibitors of proteinases
-
Laskowski M., Kato I. Protein inhibitors of proteinases. Annu. Rev. Biochem. 1980, 149:593-626.
-
(1980)
Annu. Rev. Biochem.
, vol.149
, pp. 593-626
-
-
Laskowski, M.1
Kato, I.2
-
37
-
-
0002445959
-
Amino acid sequence of serine protease inhibitor CI-1 from barley. Homology with inhibitor CI-2, potato inhibitor I, and leech eglin
-
Svendsen I., Boisen S., Hejgaard J. Amino acid sequence of serine protease inhibitor CI-1 from barley. Homology with inhibitor CI-2, potato inhibitor I, and leech eglin. Carlsberg Res. Commun. 1982, 47:45-53.
-
(1982)
Carlsberg Res. Commun.
, vol.47
, pp. 45-53
-
-
Svendsen, I.1
Boisen, S.2
Hejgaard, J.3
-
38
-
-
0014023632
-
The determination of the concentration of hydrolytic enzyme solutions: α-chymotrypsin, trypsin, papain, elastase, subtilisin, and acetylcholinesterase
-
Bender M.L., Begué-Cantón M.L., Blakeley R.L., Brubacher L.J., Feder J., Gunter CR., Kézdy F.J., Killheffer J.V., Marshall T.H., Miller C.G., Roeske R.W., Stoops J.K. The determination of the concentration of hydrolytic enzyme solutions: α-chymotrypsin, trypsin, papain, elastase, subtilisin, and acetylcholinesterase. J. Am. Chem. Soc. 1966, 88(24):5890-5913.
-
(1966)
J. Am. Chem. Soc.
, vol.88
, Issue.24
, pp. 5890-5913
-
-
Bender, M.L.1
Begué-Cantón, M.L.2
Blakeley, R.L.3
Brubacher, L.J.4
Feder, J.5
Gunter, C.R.6
Kézdy, F.J.7
Killheffer, J.V.8
Marshall, T.H.9
Miller, C.G.10
Roeske, R.W.11
Stoops, J.K.12
-
39
-
-
0030589046
-
Active-site titration of serine proteases using a fluoride ion selective electrode and sulfonyl fluoride inhibitors
-
Hsia C., Ganshaw G., Paech C., Murray C. Active-site titration of serine proteases using a fluoride ion selective electrode and sulfonyl fluoride inhibitors. Anal. Biochem. 1996, 242:221-227.
-
(1996)
Anal. Biochem.
, vol.242
, pp. 221-227
-
-
Hsia, C.1
Ganshaw, G.2
Paech, C.3
Murray, C.4
-
40
-
-
0026638586
-
Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching
-
Grøn H., Meldal M., Breddam K. Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching. Biochemistry 1992, 31:6011-6018.
-
(1992)
Biochemistry
, vol.31
, pp. 6011-6018
-
-
Grøn, H.1
Meldal, M.2
Breddam, K.3
-
41
-
-
0029521168
-
Studies of binding sites in the subtilisin from Bacillus lentus by means of site directed mutagenesis and kinetic investigations
-
Plenum, New York
-
Grøn H., Bech L.M., Sørensen S.B., Meldal M., Breddam K. Studies of binding sites in the subtilisin from Bacillus lentus by means of site directed mutagenesis and kinetic investigations. Subtilisin Enzymes: Practical Protein Engineering, Advances in Experimental Medicine and Biology 1996, vol. 379:105-112. Plenum, New York.
-
(1996)
Subtilisin Enzymes: Practical Protein Engineering, Advances in Experimental Medicine and Biology
, vol.379
, pp. 105-112
-
-
Grøn, H.1
Bech, L.M.2
Sørensen, S.B.3
Meldal, M.4
Breddam, K.5
-
42
-
-
0027479593
-
Engineering a novel specificity in subtilisin BPN'
-
Rheinnecker M., Baker G., Eder J., Fersht A.R. Engineering a novel specificity in subtilisin BPN'. Biochemistry 1993, 32:1199-1203.
-
(1993)
Biochemistry
, vol.32
, pp. 1199-1203
-
-
Rheinnecker, M.1
Baker, G.2
Eder, J.3
Fersht, A.R.4
-
43
-
-
0026754643
-
Interdependency of binding sub-sites in subtilisin
-
Grøn H., Breddam K. Interdependency of binding sub-sites in subtilisin. Biochemistry 1992, 31:8967-8971.
-
(1992)
Biochemistry
, vol.31
, pp. 8967-8971
-
-
Grøn, H.1
Breddam, K.2
-
44
-
-
0028874551
-
Designing subtilisin BPN' to cleave substrates containing dibasic residues
-
Ballinger M.D., Tom J., Wells J.A. Designing subtilisin BPN' to cleave substrates containing dibasic residues. Biochemistry 1995, 34:13312-13319.
-
(1995)
Biochemistry
, vol.34
, pp. 13312-13319
-
-
Ballinger, M.D.1
Tom, J.2
Wells, J.A.3
-
45
-
-
0026771463
-
Protein engineering of the high-alkaline serine protease PB92 from Bacillus alcalophilus: functional and structural consequences of mutation at the S4 substrate binding pocket
-
Teplyakov A.V., van der Laan J.C., Lammers A.A., Kelders H., Kalk K.H., Misset O., Mulleners L., Dijkstra B.W. Protein engineering of the high-alkaline serine protease PB92 from Bacillus alcalophilus: functional and structural consequences of mutation at the S4 substrate binding pocket. Protein Eng. 1992, 5:413-420.
-
(1992)
Protein Eng.
, vol.5
, pp. 413-420
-
-
Teplyakov, A.V.1
van der Laan, J.C.2
Lammers, A.A.3
Kelders, H.4
Kalk, K.H.5
Misset, O.6
Mulleners, L.7
Dijkstra, B.W.8
-
46
-
-
0027819716
-
Mutational replacements of the amino acid residues forming the hydrophobic S4 binding pocket of subtilisin 309 from Bacillus lentus
-
Sørensen S.B., Bech L.M., Meldal M., Breddam K. Mutational replacements of the amino acid residues forming the hydrophobic S4 binding pocket of subtilisin 309 from Bacillus lentus. Biochemistry 1993, 32:8994-8999.
-
(1993)
Biochemistry
, vol.32
, pp. 8994-8999
-
-
Sørensen, S.B.1
Bech, L.M.2
Meldal, M.3
Breddam, K.4
-
47
-
-
0001463338
-
Designing substrate specificity by protein engineering of electrostatic interactions
-
Wells J.A., Powers D.B., Bott R.R., Graycar T.P., Estell D.A. Designing substrate specificity by protein engineering of electrostatic interactions. Proc. Natl. Acad. Sci. USA 1987, 84:1219-1223.
-
(1987)
Proc. Natl. Acad. Sci. USA
, vol.84
, pp. 1219-1223
-
-
Wells, J.A.1
Powers, D.B.2
Bott, R.R.3
Graycar, T.P.4
Estell, D.A.5
-
48
-
-
0028013522
-
Variants of subtilisin BPN' with altered specificity profiles
-
Rheinnecker M., Eder J., Pandey P.S., Fersht A.R. Variants of subtilisin BPN' with altered specificity profiles. Biochemistry 1994, 33:221-225.
-
(1994)
Biochemistry
, vol.33
, pp. 221-225
-
-
Rheinnecker, M.1
Eder, J.2
Pandey, P.S.3
Fersht, A.R.4
-
49
-
-
0028914722
-
Structural basis of substrate specificity in the serine proteases
-
Perona J.J., Craik C.S. Structural basis of substrate specificity in the serine proteases. Protein Sci. 1995, 4:337-360.
-
(1995)
Protein Sci.
, vol.4
, pp. 337-360
-
-
Perona, J.J.1
Craik, C.S.2
-
50
-
-
0023394374
-
Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering
-
Wells J.A., Cunningham B.C., Graycar T.P., Estell D.A. Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering. Proc. Natl. Acad. Sci. USA 1987, 84:5167-5171.
-
(1987)
Proc. Natl. Acad. Sci. USA
, vol.84
, pp. 5167-5171
-
-
Wells, J.A.1
Cunningham, B.C.2
Graycar, T.P.3
Estell, D.A.4
-
51
-
-
0023384982
-
Engineering enzyme specificity by 'substrate-assisted catalysis'
-
Carter P., Wells J.A. Engineering enzyme specificity by 'substrate-assisted catalysis'. Science 1987, 237:394-399.
-
(1987)
Science
, vol.237
, pp. 394-399
-
-
Carter, P.1
Wells, J.A.2
-
52
-
-
0024814207
-
Engineering subtilisin BPN' for site-specific proteolysis
-
Carter P., Nilsson B., Burnier J.P., Burdick D., Wells J.A. Engineering subtilisin BPN' for site-specific proteolysis. Proteins Struct. Funct. Genet. 1989, 6:240-248.
-
(1989)
Proteins Struct. Funct. Genet.
, vol.6
, pp. 240-248
-
-
Carter, P.1
Nilsson, B.2
Burnier, J.P.3
Burdick, D.4
Wells, J.A.5
-
53
-
-
0025874449
-
Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'
-
Carter P., Abrahmsen L., Wells J.A. Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'. Biochemistry 1991, 30:6142-6148.
-
(1991)
Biochemistry
, vol.30
, pp. 6142-6148
-
-
Carter, P.1
Abrahmsen, L.2
Wells, J.A.3
-
54
-
-
0026692625
-
An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1-3)insulin-like growth factor I
-
Forsberg G., Baastrup B., Rondahl H., Holmgren E., Pohl G., Hartmanis M., Lake M. An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1-3)insulin-like growth factor I. J. Protein Chem. 1992, 11:201-211.
-
(1992)
J. Protein Chem.
, vol.11
, pp. 201-211
-
-
Forsberg, G.1
Baastrup, B.2
Rondahl, H.3
Holmgren, E.4
Pohl, G.5
Hartmanis, M.6
Lake, M.7
-
55
-
-
0029913564
-
Furilisin: a subtilisin BPN' variant engineered for cleaving tribasic substrates
-
Ballinger M.D., Tom J., Wells J.A. Furilisin: a subtilisin BPN' variant engineered for cleaving tribasic substrates. Biochemistry 1996, 35:13579-13585.
-
(1996)
Biochemistry
, vol.35
, pp. 13579-13585
-
-
Ballinger, M.D.1
Tom, J.2
Wells, J.A.3
-
56
-
-
33845281839
-
Peptide segment coupling catalyzed by the semisynthetic enzyme thiolsubtilisin
-
Nakatsuka T., Sasaki T., Kaiser E.T. Peptide segment coupling catalyzed by the semisynthetic enzyme thiolsubtilisin. J. Am. Chem. Soc. 1987, 109:3808-3810.
-
(1987)
J. Am. Chem. Soc.
, vol.109
, pp. 3808-3810
-
-
Nakatsuka, T.1
Sasaki, T.2
Kaiser, E.T.3
-
57
-
-
0025757012
-
Engineering subtilisin and its substrates for efficient ligation of peptide bonds in aqueous solution
-
Abrahmsen L., Tom J., Bumier J., Butcher K.A., Kossiakoff A., Wells J.A. Engineering subtilisin and its substrates for efficient ligation of peptide bonds in aqueous solution. Biochemistry 1991, 30:4151-4159.
-
(1991)
Biochemistry
, vol.30
, pp. 4151-4159
-
-
Abrahmsen, L.1
Tom, J.2
Bumier, J.3
Butcher, K.A.4
Kossiakoff, A.5
Wells, J.A.6
-
58
-
-
0028577295
-
Subtiligase: a tool for semisynthesis of proteins
-
Chang T.K., Jackson D.Y., Burnier J.B., Wells J.A. Subtiligase: a tool for semisynthesis of proteins. Proc. Natl Acad. Sci. USA 1994, 91:12544-12548.
-
(1994)
Proc. Natl Acad. Sci. USA
, vol.91
, pp. 12544-12548
-
-
Chang, T.K.1
Jackson, D.Y.2
Burnier, J.B.3
Wells, J.A.4
-
59
-
-
0028518514
-
A designed peptide ligase for total synthesis of ribonuclease A with unnatural catalytic residues
-
Jackson D.Y., Burnier J., Quan C., Stanley M., Tom J., Wells J.A. A designed peptide ligase for total synthesis of ribonuclease A with unnatural catalytic residues. Science 1994, 266:243-247.
-
(1994)
Science
, vol.266
, pp. 243-247
-
-
Jackson, D.Y.1
Burnier, J.2
Quan, C.3
Stanley, M.4
Tom, J.5
Wells, J.A.6
-
60
-
-
33845185284
-
Conversion of a protease into an acyl transferase: selenolsubtilisin
-
Wu Z.-P., Hilvert D. Conversion of a protease into an acyl transferase: selenolsubtilisin. J. Am. Chem. Soc. 1989, 111:4513-4514.
-
(1989)
J. Am. Chem. Soc.
, vol.111
, pp. 4513-4514
-
-
Wu, Z.-P.1
Hilvert, D.2
-
61
-
-
0024992830
-
Selenosubtilisin as a glutathione peroxidase mimic
-
Wu Z.-P., Hilvert D. Selenosubtilisin as a glutathione peroxidase mimic. J. Am. Chem. Soc. 1990, 112:5647-5648.
-
(1990)
J. Am. Chem. Soc.
, vol.112
, pp. 5647-5648
-
-
Wu, Z.-P.1
Hilvert, D.2
-
62
-
-
0027732153
-
Peroxide dependence of the semisynthetic enzyme selenosubtilisin
-
Bell I.M., Fisher M.L., Wu Z.P., Hilvert D. Peroxide dependence of the semisynthetic enzyme selenosubtilisin. Biochemistry 1993, 32:13969-13973.
-
(1993)
Biochemistry
, vol.32
, pp. 13969-13973
-
-
Bell, I.M.1
Fisher, M.L.2
Wu, Z.P.3
Hilvert, D.4
-
63
-
-
0025998717
-
Homology modeling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteases
-
Siezen R.J., de Vos W.M., Leunissen A.M., Dijkstra B.W. Homology modeling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteases. Protein Eng. 1991, 4:719-737.
-
(1991)
Protein Eng.
, vol.4
, pp. 719-737
-
-
Siezen, R.J.1
de Vos, W.M.2
Leunissen, A.M.3
Dijkstra, B.W.4
-
64
-
-
0029548160
-
Crystal structure from the alkaline proteases savinase and esperase from Bacillus lentus
-
Plenum, New York
-
Betzel C., Klupsch S., Branner S., Wilson K.S. Crystal structure from the alkaline proteases savinase and esperase from Bacillus lentus. Subtilisin Enzymes: Practical Protein Engineering, Advances in Experimental Medicine and Biology 1996, vol. 379:49-61. Plenum, New York.
-
(1996)
Subtilisin Enzymes: Practical Protein Engineering, Advances in Experimental Medicine and Biology
, vol.379
, pp. 49-61
-
-
Betzel, C.1
Klupsch, S.2
Branner, S.3
Wilson, K.S.4
-
66
-
-
0002529802
-
Homology analysis of the propeptides of subtilisin-like serine proteases (subtilases)
-
Springer, New York, U. Shinde, M. Inouye (Eds.)
-
Siezen R.J., Leunissen J.A.M., Shinde U. Homology analysis of the propeptides of subtilisin-like serine proteases (subtilases). Intramolecular Chaperones and Protein Folding 1995, 233-256. Springer, New York. U. Shinde, M. Inouye (Eds.).
-
(1995)
Intramolecular Chaperones and Protein Folding
, pp. 233-256
-
-
Siezen, R.J.1
Leunissen, J.A.M.2
Shinde, U.3
-
67
-
-
0027244542
-
Catalysis of a protein folding reaction: thermodynamic and kinetic analysis of subtilisin BPN' interactions with its propeptide fragment
-
Strausberg S., Alexander P., Wang L., Schwartz F., Bryan P. Catalysis of a protein folding reaction: thermodynamic and kinetic analysis of subtilisin BPN' interactions with its propeptide fragment. Biochemistry 1993, 32:8112-8119.
-
(1993)
Biochemistry
, vol.32
, pp. 8112-8119
-
-
Strausberg, S.1
Alexander, P.2
Wang, L.3
Schwartz, F.4
Bryan, P.5
-
68
-
-
0035976917
-
Folding pathway mediated by an intramolecular chaperone
-
Yabuta Y., Takagi H., Inouye M., Shinde U. Folding pathway mediated by an intramolecular chaperone. J. Biol. Chem. 2001, 276:44427-44434.
-
(2001)
J. Biol. Chem.
, vol.276
, pp. 44427-44434
-
-
Yabuta, Y.1
Takagi, H.2
Inouye, M.3
Shinde, U.4
-
69
-
-
0023729499
-
Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-C-subtilisin Carlsberg and CI-2-subtilisin Novo
-
McPhalen C.A., James N.G. Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-C-subtilisin Carlsberg and CI-2-subtilisin Novo. Biochemistry 1988, 27:6582-6598.
-
(1988)
Biochemistry
, vol.27
, pp. 6582-6598
-
-
McPhalen, C.A.1
James, N.G.2
-
70
-
-
0024278654
-
The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8Å and an analysis of the structural consequences of peroxide inactivation
-
Bott R., Ultsch M., Kossiakoff A., Graycar T., Katz B., Power S. The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8Å and an analysis of the structural consequences of peroxide inactivation. J. Biol. Chem. 1988, 263:7895-7906.
-
(1988)
J. Biol. Chem.
, vol.263
, pp. 7895-7906
-
-
Bott, R.1
Ultsch, M.2
Kossiakoff, A.3
Graycar, T.4
Katz, B.5
Power, S.6
-
71
-
-
0025812630
-
Refined crystal structure of the complex of subtilisin BPN' and Streptomyces subtilisin inhibitor at 1.8Å resolution
-
Takeuchi Y., Satow Y., Nakamura K.T., Mitsui Y. Refined crystal structure of the complex of subtilisin BPN' and Streptomyces subtilisin inhibitor at 1.8Å resolution. J. Mol. Biol. 1991, 221:309-325.
-
(1991)
J. Mol. Biol.
, vol.221
, pp. 309-325
-
-
Takeuchi, Y.1
Satow, Y.2
Nakamura, K.T.3
Mitsui, Y.4
-
72
-
-
0022701771
-
Refined 1.2Å crystal structure of the complex formed between subtilisin Carlsberg and the inhibitor eglin C. Molecular structure of eglin and its detailed interaction with subtilisin
-
Bode W., Papamokos E., Musil D., Seemüller U., Fritz H. Refined 1.2Å crystal structure of the complex formed between subtilisin Carlsberg and the inhibitor eglin C. Molecular structure of eglin and its detailed interaction with subtilisin. EMBO J. 1986, 5:813-818.
-
(1986)
EMBO J.
, vol.5
, pp. 813-818
-
-
Bode, W.1
Papamokos, E.2
Musil, D.3
Seemüller, U.4
Fritz, H.5
-
73
-
-
0032578355
-
The 0.78 Å structure of a serine protease: Bacillus lentus subtilisin
-
Kuhn P., Knapp M., Soltis S., Ganshaw G., Thoene M., Bott R. The 0.78 Å structure of a serine protease: Bacillus lentus subtilisin. Biochemistry 1998, 37:13446-13452.
-
(1998)
Biochemistry
, vol.37
, pp. 13446-13452
-
-
Kuhn, P.1
Knapp, M.2
Soltis, S.3
Ganshaw, G.4
Thoene, M.5
Bott, R.6
-
74
-
-
0026728830
-
Crystal structure of the high-alkaline serine protease PB92 from
-
van der Laan J.C., Teplyakov A.V., Kelders H., Kalk K.H., Misset O., Mulleners L., Dijkstra B.W. Crystal structure of the high-alkaline serine protease PB92 from. Bacillus alcalophilus. Protein Eng. 1992, 5:405-411.
-
(1992)
Bacillus alcalophilus. Protein Eng.
, vol.5
, pp. 405-411
-
-
van der Laan, J.C.1
Teplyakov, A.V.2
Kelders, H.3
Kalk, K.H.4
Misset, O.5
Mulleners, L.6
Dijkstra, B.W.7
-
75
-
-
0033543617
-
Engineered Bacillus lentus subtilisins having altered flexibility
-
Graycar T., Knapp M., Ganshaw G., Dauberman J., Bott R. Engineered Bacillus lentus subtilisins having altered flexibility. J. Mol. Biol. 1999, 292:97-109.
-
(1999)
J. Mol. Biol.
, vol.292
, pp. 97-109
-
-
Graycar, T.1
Knapp, M.2
Ganshaw, G.3
Dauberman, J.4
Bott, R.5
-
76
-
-
0029645412
-
The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'
-
Gallagher T., Gilliland G., Wang L., Bryan P. The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'. Structure 1995, 3:907-914.
-
(1995)
Structure
, vol.3
, pp. 907-914
-
-
Gallagher, T.1
Gilliland, G.2
Wang, L.3
Bryan, P.4
-
77
-
-
0032553556
-
The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0Å resolution
-
Jian S.C., Shinde U., Li Y., Inouye M., Berman H.M. The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0Å resolution. J. Mol. Bio. 1998, 284:137-144.
-
(1998)
J. Mol. Bio.
, vol.284
, pp. 137-144
-
-
Jian, S.C.1
Shinde, U.2
Li, Y.3
Inouye, M.4
Berman, H.M.5
-
78
-
-
34247187995
-
2+-induced folding
-
2+-induced folding. The Journal of Biological Chemistry 2007, 282:8246-8255.
-
(2007)
The Journal of Biological Chemistry
, vol.282
, pp. 8246-8255
-
-
Tanaka, S.1
Saito, K.2
Chon, H.3
Matsumura, H.4
Koga, Y.5
Takano, K.6
Kanaya, S.7
-
79
-
-
0026005349
-
Importance of a distal hydrogen bonding group in stabilizing the transition state of subtilisin BPN'
-
Braxton S., Wells J.A. Importance of a distal hydrogen bonding group in stabilizing the transition state of subtilisin BPN'. J. Biol. Chem. 1991, 266:11797-11800.
-
(1991)
J. Biol. Chem.
, vol.266
, pp. 11797-11800
-
-
Braxton, S.1
Wells, J.A.2
-
80
-
-
0031569799
-
The Solution Structure of serine protease PB92 from Bacillus alcalophilus presents a rigid fold with a flexible substrate-binding site
-
Martin J.R., Mulder F.A.A., Karimi-Nejad Y., van der Zwan J., Mariani M., Schipper D., Boelens R. The Solution Structure of serine protease PB92 from Bacillus alcalophilus presents a rigid fold with a flexible substrate-binding site. Structure 1997, 5:521-532.
-
(1997)
Structure
, vol.5
, pp. 521-532
-
-
Martin, J.R.1
Mulder, F.A.A.2
Karimi-Nejad, Y.3
van der Zwan, J.4
Mariani, M.5
Schipper, D.6
Boelens, R.7
-
81
-
-
0027054919
-
Altering the Proteolytic Activity of Subtilisin through Protein Engineering
-
Graycar T., Bott R., Caldwell R., Dauberman J., Lad P., Power S., Sagar I., Silva R., Weiss G., Woodhouse L., Estell D. Altering the Proteolytic Activity of Subtilisin through Protein Engineering. Anal. NY Acad. Sci. 1992, 672:71-79.
-
(1992)
Anal. NY Acad. Sci.
, vol.672
, pp. 71-79
-
-
Graycar, T.1
Bott, R.2
Caldwell, R.3
Dauberman, J.4
Lad, P.5
Power, S.6
Sagar, I.7
Silva, R.8
Weiss, G.9
Woodhouse, L.10
Estell, D.11
-
82
-
-
0029548159
-
Structural changes leading to increased enzymatic activity in an engineered variant of Bacillus lentus subtilisin
-
Plenum, New York
-
Bott R., Dauberman J., Wilson L., Ganshaw G., Sagar H., Graycar T., Estell D. Structural changes leading to increased enzymatic activity in an engineered variant of Bacillus lentus subtilisin. Subtilisin Enzymes: Practical Protein Engineering, Advances in Experimental Medicine and Biology 1996, vol. 379:277-283. Plenum, New York.
-
(1996)
Subtilisin Enzymes: Practical Protein Engineering, Advances in Experimental Medicine and Biology
, vol.379
, pp. 277-283
-
-
Bott, R.1
Dauberman, J.2
Wilson, L.3
Ganshaw, G.4
Sagar, H.5
Graycar, T.6
Estell, D.7
-
84
-
-
0002568699
-
Fermentation of Bacillus
-
American Society for Microbiology, Washington DC, A. Sonenshein, J. Hoch, R. Losick (Eds.)
-
Arbige M.V., Bulthuis B.A., Schultz J., Crabb D. Fermentation of Bacillus. Bacillus subtilis and other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics 1993, 871-895. American Society for Microbiology, Washington DC. A. Sonenshein, J. Hoch, R. Losick (Eds.).
-
(1993)
Bacillus subtilis and other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics
, pp. 871-895
-
-
Arbige, M.V.1
Bulthuis, B.A.2
Schultz, J.3
Crabb, D.4
-
85
-
-
0028032419
-
Peptide mapping of subtilisins as a practical tool for locating protein sequence errors during extensive protein engineering projects
-
Christianson T., Paech C. Peptide mapping of subtilisins as a practical tool for locating protein sequence errors during extensive protein engineering projects. Anal. Biochem. 1994, 223:119-129.
-
(1994)
Anal. Biochem.
, vol.223
, pp. 119-129
-
-
Christianson, T.1
Paech, C.2
-
86
-
-
0023644876
-
Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli
-
Ikemura H., Takagi H., Inouye M. Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli. J. Biol. Chem. 1987, 262:7859-7864.
-
(1987)
J. Biol. Chem.
, vol.262
, pp. 7859-7864
-
-
Ikemura, H.1
Takagi, H.2
Inouye, M.3
-
87
-
-
0028067179
-
Autoprocessing of prothiolsubtilisin E in which active-site serine 221 is altered to cysteine. J
-
Li Y., Inouye M. Autoprocessing of prothiolsubtilisin E in which active-site serine 221 is altered to cysteine. J. Biol. Chem. 1994, 269:4169-4174.
-
(1994)
Biol. Chem.
, vol.269
, pp. 4169-4174
-
-
Li, Y.1
Inouye, M.2
-
88
-
-
0028223248
-
The structural and functional organization of intramolecular chaperones: the N-terminal propeptides which mediate protein folding
-
Shinde U., Inouye M. The structural and functional organization of intramolecular chaperones: the N-terminal propeptides which mediate protein folding. J. Biochem. 1994, 115:629-636.
-
(1994)
J. Biochem.
, vol.115
, pp. 629-636
-
-
Shinde, U.1
Inouye, M.2
-
89
-
-
39049125071
-
Bacillus protein secretion: an unfolding story
-
Harwood C.R., Cranenburgh R. Bacillus protein secretion: an unfolding story. Trends Microbiol. 2008, 16:73-79.
-
(2008)
Trends Microbiol.
, vol.16
, pp. 73-79
-
-
Harwood, C.R.1
Cranenburgh, R.2
-
90
-
-
0016910644
-
Genetics of bacterial sporulation
-
Hoch J.A. Genetics of bacterial sporulation. Adv. Genet. 1976, 18:69-98.
-
(1976)
Adv. Genet.
, vol.18
, pp. 69-98
-
-
Hoch, J.A.1
-
91
-
-
0017060173
-
Genetic aspects of bacterial endospore formation
-
Piggot P.J., Coote J.G. Genetic aspects of bacterial endospore formation. Bacteriol. Rev. 1976, 40:908-962.
-
(1976)
Bacteriol. Rev.
, vol.40
, pp. 908-962
-
-
Piggot, P.J.1
Coote, J.G.2
-
92
-
-
0017652017
-
Extracellular enzyme synthesis in the genus
-
Priest F.G. Extracellular enzyme synthesis in the genus. Bacillus. Bacteriol. Rev. 1977, 41:711-753.
-
(1977)
Bacillus. Bacteriol. Rev.
, vol.41
, pp. 711-753
-
-
Priest, F.G.1
-
93
-
-
0018838184
-
Extracellular proteases modify cell wall turnover in Bacillus subtilis
-
Jolliffe L.K., Doyle R.J., Streips U.N. Extracellular proteases modify cell wall turnover in Bacillus subtilis. J. Bacteriol. 1980, 141:1199-1208.
-
(1980)
J. Bacteriol.
, vol.141
, pp. 1199-1208
-
-
Jolliffe, L.K.1
Doyle, R.J.2
Streips, U.N.3
-
94
-
-
0021228872
-
Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation
-
Yang M.Y., Ferrari E., Henner D.J. Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation. J. Bacteriol. 1984, 160:15-21.
-
(1984)
J. Bacteriol.
, vol.160
, pp. 15-21
-
-
Yang, M.Y.1
Ferrari, E.2
Henner, D.J.3
-
95
-
-
0030854244
-
Allergy risk in an enzyme producing plant: a retrospective follow up study
-
Johnsen C.R., Sørensen T.B., Ingemann Larsen A., Bertelsen Secher A., Andreasen E., Kofoed G.S., Fredslund Nielsen L., Gyntelberg F. Allergy risk in an enzyme producing plant: a retrospective follow up study. Occup. Environ. Med. 1997, 54:671-675.
-
(1997)
Occup. Environ. Med.
, vol.54
, pp. 671-675
-
-
Johnsen, C.R.1
Sørensen, T.B.2
Ingemann Larsen, A.3
Bertelsen Secher, A.4
Andreasen, E.5
Kofoed, G.S.6
Fredslund Nielsen, L.7
Gyntelberg, F.8
-
96
-
-
0033677213
-
CD4+ T-cell epitope determination using unexposed human donor peripheral blood mononuclear cells
-
Stickler M.M., Estell D.A., Harding F.A. CD4+ T-cell epitope determination using unexposed human donor peripheral blood mononuclear cells. J. Immunother. 2000, 23:654-660.
-
(2000)
J. Immunother.
, vol.23
, pp. 654-660
-
-
Stickler, M.M.1
Estell, D.A.2
Harding, F.A.3
-
97
-
-
0034731382
-
Protein engineering of subtilisin
-
Bryan P.N. Protein engineering of subtilisin. Biochim. Biophys. Acta 2000, 1543:203-222.
-
(2000)
Biochim. Biophys. Acta
, vol.1543
, pp. 203-222
-
-
Bryan, P.N.1
-
98
-
-
0035033318
-
Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes
-
Wlodawer A., Li M., Dauter Z., Gustchina A., Uchida K., Oyama H., Dunn B.M., Oda K. Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes. Nature Structural Biology 2001, 8:442-446.
-
(2001)
Nature Structural Biology
, vol.8
, pp. 442-446
-
-
Wlodawer, A.1
Li, M.2
Dauter, Z.3
Gustchina, A.4
Uchida, K.5
Oyama, H.6
Dunn, B.M.7
Oda, K.8
-
99
-
-
0035951107
-
Inhibitor complexes of the Pseudomonas serine-carboxyl proteinase
-
Wlodawer A., Li M., Gustchina A., Dauter Z., Uchida K., Oyama H., Goldfarb N.E., Dunn B.M., Oda K. Inhibitor complexes of the Pseudomonas serine-carboxyl proteinase. Biochemistry 2001, 40:15602-15611.
-
(2001)
Biochemistry
, vol.40
, pp. 15602-15611
-
-
Wlodawer, A.1
Li, M.2
Gustchina, A.3
Dauter, Z.4
Uchida, K.5
Oyama, H.6
Goldfarb, N.E.7
Dunn, B.M.8
Oda, K.9
|