Engineered Bacillus lentus subtilisins having altered flexibility

Journal of Molecular Biology

Volumn 292, Issue 1, 1999, Pages 97-109

  Graycar, Thomas ^a   Knapp, Mark ^a   Ganshaw, Grant ^a   Dauberman, Judy ^a   Bott, Richard ^a  

^a GENENCOR INTERNATIONAL INC   (United States)

Author keywords

Lattice deformations; Protein flexibility; Subtilisin protein engineering; X ray crystallography

Indexed keywords

BACTERIAL ENZYME; MUTANT PROTEIN; SUBTILISIN;

ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; EGGERTHELLA LENTA; ENZYME ACTIVITY; ENZYME CONFORMATION; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; STATISTICAL ANALYSIS; X RAY CRYSTALLOGRAPHY;

BACILLUS LENTUS;

EID: 0033543617     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.3033     Document Type: Article

References (21)

1. ®from Bacillus lentus at 1.4 Å resolution. J. Mol. Biol. 223:1992;427-445.
2. Blundell, T. Johnson, L. N. 1971, Protein Crystallography, Academic Press, New York.
3. Bone R., Shenvi A. B., Kettner C. A., Agard D. A. Refined structure of alpha-lytic protease at 1.7 Angstroms resolution. Analysis of hydrogen bonding and solvent structure. Biochemistry. 26:1987;7609-7614.
4. Bott R., Frane J. Incorporation of crystallographic temperature factors in the statistical analysis of protein tertiary structures. Protein Eng. 3:1990;649-657.
5. Bott R., Ultsch M., Kossiakoff A., Graycar T., Katz B., Power S. The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 Å and an analysis of the structural consequences of peroxide inactivation. J. Biol. Chem. 263:1988;7895-7906.
6. Bott R., Dauberman J., Caldwell R., Mitchinson C., Wilson L., Schmidt B., Simpson C., Power S., Lad P., Sagar I. H., Craycar T., Estell D. Using structural comparison as a guide in protein engineering. Ann. N.Y. Acad. Sci. 672:1992;10-19.
7. Bryan P. N., Rollence M. L., Pantiliano M. W., Wood J., Finzel B. C., Gilliland G., Howard A. J., Poulos T. L. Proteases of enhanced stability: characterization of a thermostable variant of subtilisin. Proteins: Struct. Funct. Genet. 1:1986;326-334.
8. Gallegher T., Oliver J., Bott R., Betzel C., Gilliland G. L. Subtilisin BPN′ at 1.6 Å resolution: analysis for discrete disorder and comparison of crystal forms. Acta Crystallog. sect. D. 52:1996;1125-1135.
9. Gilliland G. L., Gallagher D. T., Bryan P. Bott R., Betzel C. Subtilisin Enzymes: Practical Protein Engineering. 1996;159-169 Plenum Press, New York.
10. Goddette D. W., Paech C., Yang S. S., Mielenz J. R., Bystroff C., Wilke M. E., Fletterick R. J. The crystal structure of the Bacillus lentus alkaline protease subtilisin BL, at 1.4 Å resolution. J. Mol. Biol. 228:1992;580-595.
11. Graycar T. P., Bott R. R., Caldwell R. M., Dauberman J. L., Lad P. J., Power S. D., Sagar H. I., Silva R. A., Weiss G. L., Woodhouse L. R., Estell D. E. Altering the proteolytic activity of subtilisin through protein engineering. Anal. NY Acad. Sci. 672:1992;71-79.
12. Graycar T., Bott R., Wilson L. "Subtilisin Variants". WO 9510615 application. 1995.
13. Gron H., Meldal M., Breddam K. Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching. Biochemistry. 31:1992;6011-6018.
14. Hendrickson W. A., Konnert J. Srinivasan R. Biomolecular Structure Conformation Function and Evolution. 1981;43-47 Pergamon Press, Oxford.
15. Lange G., Betzel C., Branner S., Wilson K. S. Crystallographic studies of savinase, a subtilisin-like proteinase at pH 10.5. Eur. J. Biochem. 224:1994;507-518.
16. McPhalen C. A., James M. N. G. Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-C-subtilisin Carlsberg and CI-2-subtilisin Novo. Biochemistry. 27:1988;6582-6598.
17. Mulder F. A. A., Schipper D., Bott R., Boelens R. Altered flexibility in the substrate-binding site of related native and engineered high-alkaline Bacillus subtilisin. J. Mol. Biol. 292:1999;111-123.
18. North A. T. C., Phillips D. C., Matthews F. S. A semi emperical method of absorption correction. Acta Crystallog. sect. A. 24:1968;351-359.
19. Siezen R. J., Leunissen J. A. M. The superfamily of subtilisin-like serine proteases. Protein Sci. 6:1997;501-523.
20. van der Laan J. M., Teplyakov A. V., Kelder H., Kalk K. H., Misset O., Mulleners L. J. S. M., Dijsktra B. W. Crystal structure of the high alkaline serine protease PB92 from Bacillus acalophilu. Protein Eng. 5:1992;405-411.
21. Wells J. A., Estell D. A. Subtilisin- an enzyme designed to be protein engineered. Trends Biochem. Sci. 13:1988;291-297.