Ghrelin inhibits the apoptosis of MC3T3-E1 cells through ERK and AKT signaling pathway

Toxicology and Applied Pharmacology

Volumn 272, Issue 3, 2013, Pages 591-597

  Liang, Qiu Hua ^a   Liu, Yuan ^a   Wu, Shan Shan ^a   Cui, Rong Rong ^a   Yuan, Ling Qing ^a   Liao, Er Yuan ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Apoptosis; Ghrelin; Osteoblast; Signaling pathway

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 2 MORPHOLINO 8 PHENYLCHROMONE; CASPASE 3; GHRELIN; GHRELIN RECEPTOR; PROTEIN BAX; PROTEIN BCL 2; PROTEIN KINASE B; SMALL INTERFERING RNA;

ANIMAL CELL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PHOSPHORYLATION; MOUSE; NICK END LABELING; NONHUMAN; OSSIFICATION; OSTEOBLAST; PROTEIN EXPRESSION; PROTEIN FUNCTION; RECEPTOR DOWN REGULATION; RECEPTOR UPREGULATION;

APOPTOSIS; GHRELIN; OSTEOBLAST; SIGNALING PATHWAY;

3T3 CELLS; AMINO ACID SEQUENCE; ANIMALS; APOPTOSIS; GHRELIN; MAP KINASE SIGNALING SYSTEM; MICE; MOLECULAR SEQUENCE DATA; OSTEOBLASTS; PHOSPHORYLATION; PROTO-ONCOGENE PROTEINS C-AKT;

EID: 84884707343     PISSN: 0041008X     EISSN: 10960333     Source Type: Journal    
DOI: 10.1016/j.taap.2013.07.018     Document Type: Article

References (36)

1. Baldanzi G., Filigheddu N., Cutrupi S., Catapano F., Bonissoni S., Fubini A., Malan D., Baj G., Granata R., Broglio F., Papotti M., Surico N., Bussolino F., Isgaard J., Deghenghi R., Sinigaglia F., Prat M., Muccioli G., Ghigo E., Graziani A. Ghrelin and des-acyl ghrelin inhibit cell death in cardiomyocytes and endothelial cells through ERK1/2 and PI 3-kinase/AKT. J. Cell Biol. 2002, 159:1029-1037.
2. Cantrell D.A. Phosphoinositide 3-kinase signalling pathways. J. Cell Sci. 2001, 114:1439-1445.
3. Chua C.C., Chua B.H., Chen Z., Landy C., Hamdy R.C. Dexamethasone induces caspase activation in murine osteoblastic MC3T3-E1 cells. Biochim. Biophys. Acta 2003, 1642:79-85.
4. Chung H., Kim E., Lee D.H., Seo S., Ju S., Lee D., Kim H., Park S. Ghrelin inhibits apoptosis in hypothalamic neuronal cells during oxygen-glucose deprivation. Endocrinology 2007, 148:148-159.
5. Costa J.L., Naot D., Lin J.M., Watson M., Callon K.E., Reid I.R., Grey A.B., Cornish J. Ghrelin is an osteoblast mitogen and increases osteoclastic bone resorption in vitro. Int. J. Pept. 2011, 2011:605193.
6. Date Y., Kojima M., Hosoda H., Sawaguchi A., Mondal M.S., Suganuma T., Matsukura S., Kangawa K., Nakazato M. Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans. Endocrinology 2000, 141:4255-4261.
7. Fukushima N., Hanada R., Teranishi H., Fukue Y., Tachibana T., Ishikawa H., Takeda S., Takeuchi Y., Fukumoto S., Kangawa K., Nagata K., Kojima M. Ghrelin directly regulates bone formation. J. Bone Miner. Res. 2005, 20:790-798.
8. Gaytan F., Barreiro M.L., Caminos J.E., Chopin L.K., Herington A.C., Morales C., Pinilla L., Paniagua R., Nistal M., Casanueva F.F., Aguilar E., Dieguez C., Tena-Sempere M. Expression of ghrelin and its functional receptor, the type 1a growth hormone secretagogue receptor, in normal human testis and testicular tumors. J. Clin. Endocrinol. Metab. 2004, 89:400-409.
9. Granata R., Settanni F., Biancone L., Trovato L., Nano R., Bertuzzi F., Destefanis S., Annunziata M., Martinetti M., Catapano F., Ghe C., Isgaard J., Papotti M., Ghigo E., Muccioli G. Acylated and unacylated ghrelin promote proliferation and inhibit apoptosis of pancreatic beta-cells and human islets: involvement of 3',5'-cyclic adenosine monophosphate/protein kinase A, extracellular signal-regulated kinase 1/2, and phosphatidyl inositol 3-Kinase/Akt signaling. Endocrinology 2007, 148:512-529.
10. Gu Y.X., Du J., Si M.S., Mo J.J., Qiao S.C., Lai H.C. The roles of PI3K/Akt signaling pathway in regulating MC3T3-E1 preosteoblast proliferation and differentiation on SLA and SLActive titanium surfaces. J. Biomed. Mater. Res. A 2013, 101:748-754.
11. Hock J.M., Krishnan V., Onyia J.E., Bidwell J.P., Milas J., Stanislaus D. Osteoblast apoptosis and bone turnover. J. Bone Miner. Res. 2001, 16:975-984.
12. Howard A.D., Feighner S.D., Cully D.F., Arena J.P., Liberator P.A., Rosenblum C.I., Hamelin M., Hreniuk D.L., Palyha O.C., Anderson J., Paress P.S., Diaz C., Chou M., Liu K.K., McKee K.K., Pong S.S., Chaung L.Y., Elbrecht A., Dashkevicz M., Heavens R., Rigby M., Sirinathsinghji D.J., Dean D.C., Melillo D.G., Patchett A.A., Nargund R., Griffin P.R., DeMartino J.A., Gupta S.K., Schaeffer J.M., Smith R.G., Van der Ploeg L.H. A receptor in pituitary and hypothalamus that functions in growth hormone release. Science 1996, 273:974-977.
13. Jilka R.L., Weinstein R.S., Parfitt A.M., Manolagas S.C. Quantifying osteoblast and osteocyte apoptosis: challenges and rewards. J. Bone Miner. Res. 2007, 22:1492-1501.
14. Kim M.S., Yoon C.Y., Jang P.G., Park Y.J., Shin C.S., Park H.S., Ryu J.W., Pak Y.K., Park J.Y., Lee K.U., Kim S.Y., Lee H.K., Kim Y.B., Park K.S. The mitogenic and antiapoptotic actions of ghrelin in 3T3-L1 adipocytes. Mol. Endocrinol. 2004, 18:2291-2301.
15. Kim S.W., Her S.J., Park S.J., Kim D., Park K.S., Lee H.K., Han B.H., Kim M.S., Shin C.S., Kim S.Y. Ghrelin stimulates proliferation and differentiation and inhibits apoptosis in osteoblastic MC3T3-E1 cells. Bone 2005, 37:359-369.
16. Kim H., Rafiuddin-Shah M., Tu H.C., Jeffers J.R., Zambetti G.P., Hsieh J.J., Cheng E.H. Hierarchical regulation of mitochondrion-dependent apoptosis by BCL-2 subfamilies. Nat. Cell Biol. 2006, 8:1348-1358.
17. Kitamura T., Itoh M., Noda T., Matsuura M., Wakabayashi K. Combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on intestinal tumorigenesis in adenomatous polyposis coli gene knockout mice. Int. J. Cancer 2004, 109:576-580.
18. Kojima M., Hosoda H., Date Y., Nakazato M., Matsuo H., Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999, 402:656-660.
19. Kui L., Weiwei Z., Ling L., Daikun H., Guoming Z., Linuo Z., Renming H. Ghrelin inhibits apoptosis induced by high glucose and sodium palmitate in adult rat cardiomyocytes through the PI3K-Akt signaling pathway. Regul. Pept. 2009, 155:62-69.
20. Liang Q.H., Jiang Y., Zhu X., Cui R.R., Liu G.Y., Liu Y., Wu S.S., Liao X.B., Xie H., Zhou H.D., Wu X.P., Yuan L.Q., Liao E.Y. Ghrelin attenuates the osteoblastic differentiation of vascular smooth muscle cells through the ERK pathway. PLoS One 2012, 7:e33126.
21. Maccarinelli G., Sibilia V., Torsello A., Raimondo F., Pitto M., Giustina A., Netti C., Cocchi D. Ghrelin regulates proliferation and differentiation of osteoblastic cells. J. Endocrinol. 2005, 184:249-256.
22. Mazzocchi G., Neri G., Rucinski M., Rebuffat P., Spinazzi R., Malendowicz L.K., Nussdorfer G.G. Ghrelin enhances the growth of cultured human adrenal zona glomerulosa cells by exerting MAPK-mediated proliferogenic and antiapoptotic effects. Peptides 2004, 25:1269-1277.
23. McKee K.K., Palyha O.C., Feighner S.D., Hreniuk D.L., Tan C.P., Phillips M.S., Smith R.G., Van der Ploeg L.H., Howard A.D. Molecular analysis of rat pituitary and hypothalamic growth hormone secretagogue receptors. Mol. Endocrinol. 1997, 11:415-423.
24. McKee K.K., Tan C.P., Palyha O.C., Liu J., Feighner S.D., Hreniuk D.L., Smith R.G., Howard A.D., Van der Ploeg L.H. Cloning and characterization of two human G protein-coupled receptor genes (GPR38 and GPR39) related to the growth hormone secretagogue and neurotensin receptors. Genomics 1997, 46:426-434.
25. Nikolopoulos D., Theocharis S., Kouraklis G. Ghrelin, another factor affecting bone metabolism. Med. Sci. Monit. 2010, 16:RA147-RA162.
26. Olney R.C. Regulation of bone mass by growth hormone. Med. Pediatr. Oncol. 2003, 41:228-234.
27. Rodriguez A., Gomez-Ambrosi J., Catalan V., Rotellar F., Valenti V., Silva C., Mugueta C., Pulido M.R., Vazquez R., Salvador J., Malagon M.M., Colina I., Fruhbeck G. The ghrelin O-acyltransferase-ghrelin system reduces TNF-alpha-induced apoptosis and autophagy in human visceral adipocytes. Diabetologia 2012, 55:3038-3050.
28. Sakata I., Nakamura K., Yamazaki M., Matsubara M., Hayashi Y., Kangawa K., Sakai T. Ghrelin-producing cells exist as two types of cells, closed- and opened-type cells, in the rat gastrointestinal tract. Peptides 2002, 23:531-536.
29. Shuto Y., Shibasaki T., Wada K., Parhar I., Kamegai J., Sugihara H., Oikawa S., Wakabayashi I. Generation of polyclonal antiserum against the growth hormone secretagogue receptor (GHS-R): evidence that the GHS-R exists in the hypothalamus, pituitary and stomach of rats. Life Sci. 2001, 68:991-996.
30. van der Velde M., Delhanty P., van der Eerden B., van der Lely A.J., van Leeuwen J. Ghrelin and bone. Vitam. Horm. 2008, 77:239-258.
31. Xie H., Yuan L.Q., Luo X.H., Huang J., Cui R.R., Guo L.J., Zhou H.D., Wu X.P., Liao E.Y. Apelin suppresses apoptosis of human osteoblasts. Apoptosis 2007, 12:247-254.
32. Xie H., Tang S.Y., Li H., Luo X.H., Yuan L.Q., Wang D., Liao E.Y. l-carnitine protects against apoptosis of murine MC3T3-E1 osteoblastic cells. Amino Acids 2008, 35:419-423.
33. Xie H., Tang L.L., Luo X.H., Wu X.Y., Wu X.P., Zhou H.D., Yuan L.Q., Liao E.Y. Suppressive effect of dexamethasone on TIMP-1 production involves murine osteoblastic MC3T3-E1 cell apoptosis. Amino Acids 2010, 38:1145-1153.
34. 2-induced oxidative damage-implications for the treatment of osteoporosis. Free Radic. Biol. Med. 2011, 50:1314-1323.
35. Yuan L.Q., Liu Y.S., Luo X.H., Guo L.J., Xie H., Lu Y., Wu X.P., Liao E.Y. Recombinant tissue metalloproteinase inhibitor-3 protein induces apoptosis of murine osteoblast MC3T3-E1. Amino Acids 2008, 35:123-127.
36. Zhu X., Jiang Y., Shan P.F., Shen J., Liang Q.H., Cui R.R., Liu Y., Liu G.Y., Wu S.S., Lu Q., Xie H., Liu Y.S., Yuan L.Q., Liao E.Y. Vaspin attenuates the apoptosis of human osteoblasts through ERK signaling pathway. Amino Acids 2013, 44:961-968.