Detection of Escherichia coli O157: H7 using immunomagnetic separation and mPCR in Turkish foods of animal origin

Letters in Applied Microbiology

Volumn 57, Issue 4, 2013, Pages 373-379

  Ertas, N ^a   Gonulalan, Z ^b   Yildirim, Y ^a   Karadal, F ^c   Abay, S ^a   Al, S ^a  

^a ERCIYES UNIVERSITY   (Turkey)

^b KYRGYZ TURKISH MANAS UNIVERSITY   (Kyrgyzstan)

^c NIGDE UNIVERSITY   (Turkey)

Author keywords

Escherichia coli O157:H7; Foods of animal origin; Immunomagnetic separation; mPCR; Virulence genes

Indexed keywords

DAIRIES; DAIRY PRODUCTS; ESCHERICHIA COLI; GENES; MEATS; PUBLIC HEALTH;

ESCHERICHIA COLI O157:H7; FOODS OF ANIMAL ORIGIN; IMMUNOMAGNETIC SEPARATION; MINCED MEAT; MULTIPLEX PCR; RAW MEATS; RAW MILK CHEESE; TURKISHS; VIRULENCE FACTORS; VIRULENCE GENE;

ANIMALS;

BACTERIUM; FOOD CONSUMPTION; MEAT; MILK; PATHOGEN; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; SEPARATION; VIRULENCE;

ANIMAL PRODUCT; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; CHEESE; CONTROLLED STUDY; EAEA GENE; ESCHERICHIA COLI O157; FAST FOOD; FOOD CONTAMINATION; FOOD SAFETY; HLYA GENE; IMMUNOMAGNETIC SEPARATION; MEAT; MILK; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PUBLIC HEALTH PROBLEM; STX1 GENE; STX2 GENE;

TURKEY;

ANIMALIA; ESCHERICHIA COLI; ESCHERICHIA COLI O157:H-;

ESCHERICHIA COLI O157:H7; FOODS OF ANIMAL ORIGIN; IMMUNOMAGNETIC SEPARATION; MPCR; VIRULENCE GENES;

ANIMALS; CATTLE; DAIRY PRODUCTS; ESCHERICHIA COLI O157; ESCHERICHIA COLI PROTEINS; FOOD MICROBIOLOGY; IMMUNOMAGNETIC SEPARATION; MEAT; MULTIPLEX POLYMERASE CHAIN REACTION; TURKEY; VIRULENCE FACTORS;

EID: 84884703864     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/lam.12124     Document Type: Article

References (52)

1. Abdul-Raouf, U.M., Ammar, M.S. and Beuchat, L.R. (1996) Isolation of Escherichia coli O157:H7 from some Egyptian foods. Int J Food Microbiol 29, 423-426.
2. Abong'o, B.O. and Momba, M.N.B. (2009) Prevalence and characterization of Escherichia coli O157:H7 isolates from meat and meat products sold in Amathole District, Eastern Cape Province of South Africa. Food Microbiol 26, 173-176.
3. Akkaya, L., Alisarli, M., Kara, R. and Telli, L. (2007) The prevalence of E. coli O157:H7 in raw milks and cheeses sold in Afyonkarahisar province, Turkey. YYU Vet Fak Derg 18, 1-5.
4. Akkus, F. (1996) Isolation of verocytotoxin producing E. coli O157:H7 in minced beef. PhD Thesis, Graduate School of Health Sciences, University of Ankara, Ankara.
5. Aksu, H., Arun, O.O., Aydi{dotless}n, A. and Ugur, M. (1999) Presence of Escherichia coli O157:H7 in food of animal origin. Pendik Vet Mikrobiyol Derg 30, 77-81.
6. Ansay, S.E. and Kaspar, C.W. (1997) Survey of retail cheese, dairy processing environments and raw milk for Escherichia coli O157:H7. Lett Appl Microbiol 25, 131-134.
7. Aslantas, O., Erdogan, S., Cantekin, Z., Gulacti, I. and Evrendilek, G.A. (2006) Isolation and characterization of verocytotoxin-producing Escherichia coli O157 from Turkish cattle. Int J Food Microbiol 106, 338-342.
8. Bai, J., Shi, X. and Nagaraja, T.G. (2010) A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7. J Microbiol Methods 82, 85-89.
9. Bandyopadhyay, S., Lodh, C., Rahaman, H., Bhattacharya, D., Bera, A.K., Ahmed, F.A., Mahanti, A., Samanta, I. et al. (2012) Characterization of shiga toxin producing (STEC) and enteropathogenic Escherichia coli (EPEC) in raw yak (Poephagus grunniens) milk and milk products. Res Vet Sci 93, 604-610.
10. Bentancor, A., Rumi, M.V., Carbonari, C., Gerhardt, E., Larza, M., Vilte, D.A., Creydt, V.P., Chinen, I. et al. (2012) Profile of Shiga toxin-producing Escherichia coli strains isolated from dogs and cats and genetic relationships with isolates from cattle, meat and humans. Vet Microbiol 156, 336-342.
11. Boerling, P., McEwen, S.A., Wilson, J.B., Johnson, R.P. and Gyles, C.L. (1999) Association between virulence factors of shiga toxin-producing Escherichia coli and disease in humans. J Clin Microbiol 37, 497-503.
12. Cadirci, O., Si{dotless}ri{dotless}ken, B., Inat, G. and Kevenk, T.O. (2010) The prevalence of Escherichia coli O157 and O157:H7 in ground beef and raw meatball by immunomagnetic separation and the detection of virulence genes using multiplex PCR. Meat Sci 84, 553-556.
13. Cagney, C., Crowley, H., Duffy, G., Sheridan, J.J., O'Brien, S., Carney, E., Anderson, W., McDowell, D.A. et al. (2004) Prevalence and numbers of Escherichia coli O157:H7 in minced beef and beef burgers from butcher shops and supermarkets in the Republic of Ireland. Food Microbiol 21, 203-212.
14. Cerqueria, A.M., Tibona, A. and Guth, B.E.C. (1997) High occurrence Shiga-like toxin producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil. J Food Prot 60, 177-180.
15. Chapman, P.A., Siddons, C.A., Cerdan Malo, A.T. and Harkin, M.A. (2000) A one year study of Escherichia coli O157 in raw beef and lamb products. Epidemiol Infect 124, 207-213.
16. Chapman, P.A., Chapman, P.A., Chapman, P.A. and Chapman, P.A. (2001) Escherichia coli O157 in cattle and sheep at slaughter, on beef and lamb carcasses and in raw beef and lamb products in South Yorkshire, UK. Int J Food Microbiol 64, 139-150.
17. Chinen, I., Tanoro, J.D., Miliwebsky, E., Lound, L.H., Chillemi, G., Ledri, S., Baschkier, A., Scarpin, M. et al. (2001) Isolation and characterization of Escherichia coli O157:H7 from retail meats in Argentina. J Food Prot 64, 1346-1351.
18. Chinen, I., Epszteyn, S., Melamed, C.L., Aguerre, L., Espinosa, E.M., Motter, M.M., Baschkier, A., Manfredi, E. et al. (2009) Shiga toxin-producing Escherichia coli O157 in beef and chicken burgers, and chicken carcasses in Buenos Aires, Argentina. Int J Food Microbiol 132, 167-171.
19. Coia, J.E., Johnston, Y., Nicholas, J., Steers, N.J. and Hanson, M.F. (2001) A survey of the prevalence of Escherichia coli O157 in raw meats, raw cow's milk and raw-milk cheeses in south-east Scotland. Int J Food Microbiol 66, 63-69.
20. Daly, P., Collier, T. and Doyle, S. (2002) PCR-ELISA detection of Escherichia coli in milk. Lett Appl Microbiol 34, 222-226.
21. Dontorou, C., Papadopoulou, C., Filioussis, G., Economou, V., Apostolou, I., Zakkas, G., Salamoura, A., Kansouzidou, A. et al. (2003) Isolation of Escherichia coli O157:H7 from foods in Greece. Int J Food Microbiol 82, 273-279.
22. Doyle, M.P. and Schoeni, J.L. (1987) Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry. Appl Environ Microbiol 53, 2394-2396.
23. Fantelli, K. and Stephan, R. (2001) Prevalence and characteristics of shigatoxin-producing Escherichia coli and Listeria monocytogenes strains isolated from minced meat in Switzerland. Int J Food Microbiol 70, 63-69.
24. Fedio, W.M., Jinneman, K.C., Yoshitomi, K.J., Zapata, R., Wendakoon, C.N., Browning, P. and Weagant, S.D. (2011) Detection of E. coli O157:H7 in raw ground beef by Pathatrix™immunomagnetic-separation, real-time PCR and cultural methods. Int J Food Microbiol 148, 87-92.
25. Fode-Vaughan, K.A., Maki, J.S., Benson, J.A. and Collins, M.L.P. (2003) Direct PCR detection of Escherichia coli O157:H7. Lett Appl Microbiol 37, 239-243.
26. Fratamico, P.M., Bagi, L.K. and Pepe, T. (2000) A multiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157:H7 in foods and bovine feces. J Food Prot 63, 1032-1037.
27. Fu, Z., Rogelj, S. and Kieft, T.L. (2005) Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR. Int J Food Microbiol 99, 47-57.
28. Gordillo, R., Córdoba, J.J., Andrade, M.J., Luque, M.I. and Rodríguez, M. (2011) Development of PCR assays for detection of Escherichia coli O157:H7 in meat products. Meat Sci 88, 767-773.
29. Heuvelink, A.E., Wernars, K. and De Boer, E. (1996) Occurrence of Escherichia coli O157 and other verocytotoxin producing E. coli in retail raw meats in the Netherlands. J Food Prot 59, 1267-1272.
30. Heuvelink, A.E., Bleumink, B., van den Biggelaar, F.L., Te Giffel, M.C., Beumer, R.R. and De Boer, E. (1998) Occurrence and survival of verocytotoxin-producing Escherichia coli O157 in raw cow's milk in the Netherlands. J Food Prot 61, 1597-1601.
31. Heuvelink, A.E., Zwartkruis-Nahuis, J.T.M., Beumer, R.R. and De Boer, E. (1999) Occurrence and survival of verocytotoxin-producing Escherichia coli O157 in meats obtain from retail outlets in the Netherlands. J Food Prot 62, 1115-1122.
32. Honish, L., Predy, G., Hislop, N., Chui, L., Kowalewska-Grochowska, K., Trottier, L., Kreplin, C. and Zazulak, I. (2005) An outbreak of E. coli O157:H7 hemorrhagic colitis associated with unpasteurized gouda cheese. Can J Public Health 96, 182-184.
33. Lahtia, E., Keskimaeki, M., Rantala, L., Hyvoenen, P., Siitonen, A. and Honkanen-Buzalski, T. (2001) Occurrence of Escherichia coli O157 in Finnish cattle. Vet Microbiol 79, 239-251.
34. Lekkas, C., Kakouri, A., Paleologos, E., Voutsinasa, L.P., Kontominas, M.G. and Samelis, J. (2006) Survival of Escherichia coli O157:H7 in Galotyri cheese stored at 4 and 12 1C. Food Microbiol 23, 268-276.
35. Liu, Y. and Li, Y. (2002) Detection of Escherichia coli O157:H7 using immunomagnetic separation and absorbance measurement. J Microbiol Methods 51, 369-377.
36. Magwira, C.A., Gashe, B.A. and Collison, E.K. (2005) Prevalence and antibiotic resistance profiles of Escherichia coli O157:H7 in beef products from retail outlets in Gaborone, Botswana. J Food Prot 68, 403-406.
37. Maurer, J.J., Schmidt, D., Petrosco, P., Sanchez, S., Bolton, L. and Lee, M.D. (1999) Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR. Appl Environ Microbiol 65, 2954-2960.
38. Mora, A., León, S.L., Blanco, M., Blanco, J.E., López, C., Dahbi, G., Echeita, A., González, E.A. et al. (2007) Phage types, virulence genes and PFGE profiles of Shiga toxin-producing Escherichia coli O157:H7 isolated from raw beef, soft cheese and vegetables in Lima (Peru). Int J Food Microbiol 114, 204-210.
39. Oksuz, O., Arici, M., Kurultay, S. and Gumus, T. (2004) Incidence of Escherichia coli O157 in raw milk and white pickled cheese manufactured from raw milk in Turkey. Food Control 15, 453-456.
40. Paton, A.W. and Paton, J.C. (1998) Detection and characterization of Shiga Toxigenic Escherichia coli by using Multiplex PCR Assays for stx1, stx2, eaeA, Enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J Clin Microbiol 36, 598-602.
41. Read, S.C., Gyles, C.L., Clarke, R.C., Lior, H. and McEwen, S. (1990) Prevalence of verocytotoxigenic Escherichia coli in ground beef, pork, and chicken in southwestern Ontario. Epidemiol Infect 105, 11-20.
42. Rey, J., Sa′nchez, S., Blanco, J.E., Hermoso de Mendoza, J., Hermoso de Mendoza, M., Garci{dotless}′a, A., Gil, C., Tejero, N. et al. (2006) Prevalence, serotypes and virulence genes of Shiga toxin-producing Escherichia coli isolated from ovine and caprine milk and other dairy products in Spain. Int J Food Microbiol 107, 212-217.
43. Samadpour, M., Kubler, M., Buck, F.C., Depavia, G.A., Mazengia, E., Stewart, J., Yang, P. and Alfi, D. (2002) Prevalence of shiga toxin producing Escherichia coli in ground beef and cattle faeces from King Country, Washington. J Food Prot 65, 1322-1325.
44. Sarimehmetoglu, B., Kuplulu, O. and Kaymaz, S. (1998) Isolation of Escherichia coli 0157:H7 in hamburgers and inegöl meatballs. Vet J Ankara Univ 45, 221-227.
45. Sarimehmetoglu, B., Aksoy, M.H., Ayaz, N.M., Ayaz, Y., Kuplulu, O. and Kaplan, Y.Z. (2009) Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR. Food Control 20, 357-361.
46. Signorini, M. and Tarabla, H. (2009) Quantitative risk assessment for verocytotoxigenic Escherichia coli in ground beef hamburgers in Argentina. Int J Food Microbiol 132, 153-161.
47. Su, H., Ma, Q., Shang, K., Liu, T., Yin, H. and Ai, S. (2012) Gold nanoparticles as colorimetric sensor: a case study on E. coli O157:H7 as a model for Gram-negative bacteria. Sensor Actuators B Chem 161, 298-303.
48. Tarr, P.I., Tran, N.T. and Wilson, R.A. (1999) Escherichia coli O157:H7 in retail ground beef in Seattle: results of a one year prospective study. J Food Prot 62, 133-139.
49. Tutenel, A.V., Pierard, D., Vandekerchove, D., Van Hoof, J. and De Zutter, L. (2003) Sensitivity of methods for the isolation of Escherichia coli O157 from maturely infected bovine faeces. Vet Microbiol 94, 341-346.
50. Varela-Hernández, J.J., Cabrera-Diaz, E., Cardona-López, M.A., Ibarra-Velázquez, L.M., Rangel-Villalobos, H., Castillo, A., Torres-Vitela, M.R. and Ramírez-Álvarez, A. (2007) Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 and non-O157 from beef carcasses at a slaughter plant in Mexico. Int J Food Microbiol 113, 237-241.
51. Vernozy-Rozand, C., Ray-Gueniot, S., Ragot, C., Bavai, C., Mazuy, C., Montet, M.P., Bouvet, J. and Richard, Y. (2002) Prevalence of Escherichia coli O157:H7 in industrial mince beef. Lett Appl Microbiol 35, 7-11.
52. Walsh, L., Dooge, D. and Hill, C. (1997) Screening of Escherichia coli O157:H7 in Irish ground beef using two commercial detection systems. Irish Vet J 50, 111-115.