Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR

Applied and Environmental Microbiology

Volumn 65, Issue 7, 1999, Pages 2954-2960

  Maurer, John J ^a   Schmidt, Denise ^b   Petrosko, Patricia ^b   Sanchez, Susan ^a   Bolton, Lance ^c   Lee, Margie D ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b DUQUESNE UNIVERSITY   (United States)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

O ANTIGEN;

ARTICLE; BACTERIUM IDENTIFICATION; DIAGNOSTIC ACCURACY; DNA SEQUENCE; ESCHERICHIA COLI O157; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN DETERMINATION; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS; SOUTHERN BLOTTING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA; VIBRIO CHOLERAE; YERSINIA ENTEROCOLITICA;

EID: 0032975729     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.7.2954-2960.1999     Document Type: Article

References (55)

1. Al-Hendy, A., P. Toivanen, and M. Skurnik. 1991. The effect of growth temperature on the biosynthesis of Yersinia enterocolitica O:3 lipopolysaccharide: temperature regulates the transcription of the rfb locus but not the rfa region. Microb. Pathog. 10:81-86.
2. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1992. Short protocols in molecular biology, 2nd ed. Current protocols. John Wiley and Sons, Inc. New York, N.Y.
4. Bernard P., P. Gabant, E. M. Bahassi, and M. Couturier. 1994. Positive-selection vectors using the F plasmid ccdB killer gene. Gene 148:71-74.
5. Bettelheim, K. A., H. Evangelidis, J. L. Pearce, E. Sowers, and N. A. Strockbine. 1993. Isolation of a Citrobacter freundii strain which carries the Escherichia coli O157 antigen. J. Clin. Microbiol. 31:760-761.
6. Bik, E. M., A. E. Bunschoten, R. J. Willems, A. C. Chang, and F. R. Mooi. 1996. Genetic organization and functional analysis of the otn DNA essential for cell-wall polysaccharide synthesis in Vibrio cholerae O139. Mol. Microbiol. 20:799-811.
7. Bilge S. S., J. C. Vary, Jr., S. F. Dowell, and P. I. Tarr. 1996. Role of the Escherichia coli O157:H7 O side chain in adherence and analysis of an rfb locus. Infect. Immun. 64:4795-4801.
8. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
9. Borczyk, A. A., H. Lior, and B. Ciebin. 1987. False positive identifications of Escherichia coli O157 in foods. Int. J. Food Microbiol. 4:347-349.
10. Brown, P. K., L. K. Romana, and P. R. Reeves. 1992. Molecular analysis of the rfb gene cluster of Salmonella serovar Muenchen (strain M67): genetic basis of the polymorphism between groups C2 and B. Mol. Microbiol. 6:1385-1394.
11. Chart, H., O. A. Okubadejo, and B. Rowe. 1991. The serological relationship between Escherichia coli O157 and Yersinia enterocolitica O9 using sera from patients with brucellosis. Epidemiol. Infect. 108:77-85.
12. Clarke, B. R., and C. Whitfield. 1992. Molecular cloning of the rfb region of Klebsiella pneumoniae serotype O1:K20: the rfb gene cluster is responsible for synthesis of D-galactan I O polysaccharide. J. Bacteriol. 174:4614-4621.
13. Datz, M., C. Janetzki-Mittmann, S. Franke, F. Gunzer, H. Schmidt, and H. Karch. 1996. Analysis of the enterohemorrhagic Escherichia coli O157 DNA region containing lambdoid phage gene p and Shiga-like toxin structural genes. Appl. Environ. Microbiol. 62:791-797.
14. Dev, V. J., M. Main, and I. Gould. 1991. Waterborne outbreak of Escherichia coli O157. Lancet 337:1412.
15. Dorn, C. R. 1993. Review of foodborne outbreak of Escherichia coli O157:H7 infection in the western United States. JAMA 203:1583-1587.
16. Dorn, C. R., S. Scotland, H. R. Smith, G. A. Willshaw, and B. Rowe. 1989. Properties of Vero cytotoxin-producing Escherichia coli serogroup O157:H7 of human and animal origin belonging to serotypes other than O157:H7. Epidemiol. Infect. 103:83-95.
17. Doyle, M. P. 1991. Escherichia coli O157:H7 and its significance in foods. Int. J. Food Microbiol. 12:289-302.
18. Doyle, M. P., and J. L. Schoeni. 1987. Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry. Appl. Environ. Microbiol. 53:2394-2396.
19. Goldberg, J. B., K. Hatano, G. S. Meluleni, and G. B. Pier. 1992. Cloning and surface expression of Pseudomonas aeruginosa O antigen in Escherichia coli. Proc. Natl. Acad. Sci. USA 89:10716-10720.
20. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557.
21. Hitchcock, P. J., and T. M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269-277.
22. Hobbs, M., and P. R. Reeves. 1995. Genetic organization and evolution of Yersinia pseudotuberculosis 3,6-dideoxyhexose biosynthetic genes. Biochim. Biophys. Acta 1245:273-277.
23. Jiang, X. M., B. Neal, F. Santiago, S. J. Lee, L. K. Romana, and P. R. Reeves. 1991. Structure and sequence of the rfb (O-antigen) gene cluster of Salmonella serovar typhimurium (strain LT2). Mol. Microbiol. 5:695-713.
24. -. J. Clin. Microbiol. 31:1200-1205.
25. Kido, N., M. Ohta, K. Iida, T. Hasegawa, H. Ito, Y. Arakawa, T. Komatsu, and N. Kato. 1989. Partial deletion of the cloned rfb gene of Escherichia coli O9 results in synthesis of a new O-antigenic lipopolysaccharide. J. Bacteriol. 171:3629-3633.
26. Klena, J. D., and C. A. Schnaitman. 1993. Function of the rfb gene cluster and the rfe gene in the synthesis of O antigen by Shigella dysenteriae 1. Mol. Microbiol. 9:393-402.
27. Landgraf, A., B. Reckman, and A. Pingould. 1991. Direct analysis of polymerase chain reation products using enzyme-linked immunosorbent assay techniques. Anal. Biochem. 198:86-91.
28. Lerman, V., D. Cohen, A. Gluck, E. Ohad, and I. Sechter. 1992. A cluster of cases of Escherichia coli O157 infection in a day care center in a communal settlement (kibbutz) in Israel. J. Clin. Microbiol. 30:520-521.
29. Liu, D., N. K. Verma, L. K. Romana, and P. R. Reeves. 1991. Relationships among the rfb regions of Salmonella serovars A, B, and D. J. Bacteriol. 173:4814-4819.
30. Luk, J. M. C., U. Kongmuang, P. R. Reeves, and A. A. Lindberg. 1993. Selective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D). J. Clin. Microbiol. 31:2118-2123.
31. Martin, M. L., L. D. Shipman, J. G. Wells, M. E. Potter, K. Hedberg, I. K. Wachsmuth, R. V. Tauxe, J. P. Davis, J. Arnoldi, and J. Tilleli. 1986. Isolation of Escherichia coli O157:H7 from dairy cattle associated with two cases of haemolytic uraemic syndrome. Lancet 2(8514):1043.
32. McPherson, D. F., R. Morona, D. W. Berger, K. C. Cheah, and P. A. Manning. 1991. Genetic analysis of the rfb region of Shigella flexneri encoding the Y serotype O-antigen specificity. Mol. Microbiol. 5:1491-1499.
33. Morona, R., M. S. Matthews, J. K. Morona, and M. H. Brown. 1990. Regions of the cloned Vibrio cholerae rfb genes needed to determine the Owaga form of the O-antigen. Mol. Gen. Genet. 224:405-412.
34. National Center for Biotechnology Information. 16 April 1996. [Online.] http://www.ncbi.nlm.nih.gov.
35. Okrend, A., and B. E. Rose. 1989. Isolation and identification of Escherichia coli O157:H7 from meat. USDA-FSIS Laboratory Communication no. 38, revision no. 3. Food Safety and Inspection Service, U.S. Department of Agriculture, Washington, D.C.
36. Perry, M. B., and D. R. Bundle. 1990. Antigenic relationships of the lipopolysaccharides of Escherichia hermanii strains with those of Escherichia coli O157:H7, Brucella melitensis, and Brucella abortus. Infect. Immun. 58:1391-1395.
37. Poustka, A., H. Rackwitz, A. M. Frischauf, B. Hohn, and H. Lehrach. 1984. Selective isolation of cosmid clones by homologous recombination in Escherichia coli. Proc. Natl. Acad. Sci. USA 81:4129-4133.
38. Rajakumar, K., B. H. Jost, C. Sasakawa, N. Okada, M. Yoshidawa, and B. Adler. 1994. Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence. J. Bacteriol. 176:2363-2373.
39. Samadpour, M., J. R. Ongerth, J. Liston, N. Tran, D. Nguyen, T. S. Whittam, R. A. Wilson, and P. I. Tarr. 1994. Occurrence of Shiga-like toxin-producing Escherichia coli in retail fresh seafood, beef, lamb, pork, and poultry from grocery stores in Seattle, Washington. Appl. Environ. Microbiol. 60:1038-1040.
40. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed., vol. 1. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
41. Schnaitman, C. A., and J. D. Klena. 1993. Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol. Rev. 57:665-682.
42. Stevenson, G., K. Andrianopoulos, M. Hobbs, and P. R. Reeves. 1996. Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid. J. Bacteriol. 178:4885-4893.
43. Sturm, S., B. Jann, K. Jann, P. Fortnagel, and K. N. Timmis. 1986. Genetic and biochemical analysis of Shigella dysenteriae 1 O antigen polysaccharide biosynthesis in Escherichia coli K-12: structure and functions of the rfb cluster. Microb. Pathog. 1:307-324.
44. Sugiyama, T., N. Kido, T. Komatsu, M. Ohta, and N. Kato. 1991. Expression of the cloned Escherichia coli O9 rfb gene in various mutants of Salmonella typhimurium. J. Bacteriol. 173:55-58.
45. Tsai, C. M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
46. Valvano, M. A., and J. H. Crosa. 1989. Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O7 lipopolysaccharide antigen of a human invasive strain of E. coli O7:K1. Infect. Immun. 57:937-943.
47. Verma, N. K., and P. R. Reeves. 1989. Identification and sequence of rfbS and rfbE, which determine antigenic specificity of group A and group D salmonellae. J. Bacteriol. 171:5694-5701.
48. Viret, J., S. J. Cryz, Jr., A. B. Lang, and D. Favre. 1993. Molecular cloning and characterization of the genetic determinants that express the complete Shigella serotype D (Shigella sonnei) lipopolysaccharide in heterologous live attenuated vaccine strains. Mol. Microbiol. 7:239-252.
49. Wang, L., and P. R. Reeves. 1998. Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes. Infect. Immun. 66:3545-3551.
50. Wells, J. G., L. D. Shipman, K. D. Greene, E. G. Sowers, J. H. Green, D. N. Cameron, F. P. Downes, M. L. Martin, P. M. Griffin, S. M. Ostroff, M. E. Porter, R. V. Tauxe, and I. K. Wachsmuth. 1991. Isolation of Escherichia coli serotype O157:H7 and other Shiga-like-toxin-producing E. coli from dairy cattle. J. Clin. Microbiol. 29:985-989.
51. Wilson, I. G. 1997. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 63:3741-3751.
52. Wittwer, C. T., G. C. Fillmore, and D. R. Hillyard. 1989. Automated polymerase chain reaction capillary tubes with hot air. Nucleic Acid Res. 17: 4353-4357.
53. Wyk, P., and P. Reeves. 1989. Identification and sequence of the gene for abequose synthetase, which confers antigenic specificity on group B salmonellae: homology with galactose epimerase. J. Bacteriol. 171:5687-5693.
54. Xiang, S., M. Hobbs, and P. R. Reeves. 1994. Molecular analysis of the rfb gene cluster of a group D2 Salmonella enterica strain: evidence for its origin from an insertion sequence-mediated recombination event between group E and D1 strains. J. Bacteriol. 176:4357-4365.
55. Zhang, L., P. Toivanen, and M. Skurnik. 1996. The gene cluster directing O-antigen biosynthesis in Yersinia enterocolitica serotype O:8: identification of the genes for mannose and galactose biosynthesis and the gene for the O-antigen polymerase. Microbiology 142:277-288.