Visualizing the effect of tumor microenvironments on radiation-induced cell kinetics in multicellular spheroids consisting of HeLa cells

Biochemical and Biophysical Research Communications

Volumn 439, Issue 4, 2013, Pages 453-458

  Kaida, Atsushi ^a   Miura, Masahiko ^a  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

Fluorescent ubiquitination based cell cycle indicator (Fucci); G2 arrest; Radiation; Spheroid; Tumor microenvironment

Indexed keywords

ARTICLE; CELL CYCLE G1 PHASE; CELL CYCLE PROGRESSION; CELL FRACTIONATION; CELL KINETICS; CELL PROLIFERATION; CELLS; CONFOCAL MICROSCOPE; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE IMAGING; FLUORESCENT UBIQUITINATION BASED CELL CYCLE INDICATOR; G2 PHASE CELL CYCLE CHECKPOINT; HELA CELL; HUMAN; HUMAN CELL; IRRADIATION; MONOLAYER CULTURE; MULTICELLULAR SPHEROID; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RADIATION; RADIATION INDUCED NEOPLASM; TIME LAPSE IMAGING; TUMOR MICROENVIRONMENT; TUMOR SPHEROID;

FLUORESCENT UBIQUITINATION-BASED CELL-CYCLE INDICATOR (FUCCI); G2 ARREST; RADIATION; SPHEROID; TUMOR MICROENVIRONMENT;

FLUORESCENT DYES; G2 PHASE CELL CYCLE CHECKPOINTS; HELA CELLS; HUMANS; KINETICS; SPHEROIDS, CELLULAR; TUMOR CELLS, CULTURED; TUMOR MICROENVIRONMENT; UBIQUITINATION;

EID: 84884702952     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.08.093     Document Type: Article

References (17)

1. Hanahan D., Weinberg R.A. Hallmarks of cancer: the next generation. Cell 2011, 144:646-674.
2. Harada H., Inoue M., Itasaka S., Hirota K., Morinibu A., Shinomiya K., Zeng L., Ou G., Zhu Y., Yoshimura M., McKenna W.G., Muschel R.J., Hiraoka M. Cancer cells that survive radiation therapy acquire HIF-1 activity and translocate towards tumour blood vessels. Nat. Commun. 2012, 17:783.
3. Durand R.E., Raleigh J.A. Identification of nonproliferating but viable hypoxic tumor cells in vivo. Cancer Res. 1998, 58:3547-3550.
4. Sham E., Durand R.E. Cell kinetics and repopulation parameters of irradiated xenograft tumours in SCID mice: comparison of two dose-fractionation regimens. Eur. J. Cancer 1999, 35:850-858.
5. Kimlin L.C., Casagrande G., Virador V.M. In vitro three-dimensional (3D) models in cancer research: an update. Mol. Carcinog. 2013, 52:167-182.
6. Hall E.J., Giaccia J. Spheroids: An In Vitro Model Tumor System, Radiobiology for the Radiologist 2012, Lippincott Williams & Wilkins, Philadelphia, pp. 365-367. seventh ed.
7. Lobjois V., Frongia C., Jozan S., Truchet I., Valette A. Cell cycle and apoptotic effects of SAHA are regulated by the cellular microenvironment in HCT116 multicellular tumour spheroids. Eur. J. Cancer 2009, 45:2402-2411.
8. Hirschhaeuser F., Menne H., Dittfeld C., West J., Mueller-Klieser W., Kunz-Schughart L.A. Multicellular tumor spheroids: an underestimated tool is catching up again. J. Biotechnol. 2010, 148:3-15.
9. Laurent J., Frongia C., Cazales M., Mondesert O., Ducommun B., Lobjois V. Multicellular tumor spheroid models to explore cell cycle checkpoints in 3D. BMC cancer 2013, 13:73.
10. Motoyama N., Naka K. DNA damage tumor suppressor genes and genomic instability. Curr. Opini. Genet. Dev. 2004, 14:11-16.
11. Tamamoto T., Ohnishi K., Takahashi A., Wang X., Yosimura H., Ohishi H., Uchida H., Ohnishi T. Correlation between gamma-ray-induced G2 arrest and radioresistance in two human cancer cells. Int. J. Radiat. Oncol., Biol., Phys. 1999, 44:905-909.
12. Ishikawa M., Ogihara Y., Miura M. Visualization of radiation-induced cell cycle-associated events in tumor cells expressing the fusion protein of Azami Green and the destruction box of human Geminin. Biochem. Biophys. Res. Commun. 2009, 389:426-430.
13. Kaida A., Sawai N., Sakaguchi K., Miura M. Fluorescence kinetics in HeLa cells after treatment with cell cycle arrest inducers visualized with Fucci (fluorescent ubiquitination-based cell cycle indicator). Cell Biol. Int. 2011, 35:359-363.
14. Kaida A., Miura M. Visualizing the effect of hypoxia on fluorescence kinetics in living HeLa cells using the fluorescent ubiquitination-based cell cycle indicator (Fucci). Exp. Cell Res. 2012, 318:288-297.
15. Sakaue-Sawano A., Kurokawa H., Morimura T., Hanyu A., Hama H., Osawa H., Kashiwagi S., Fukami K., Miyata T., Miyoshi H., Imamura T., Ogawa M., Masai H., Miyawaki A. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 2008, 132:487-498.
16. Hama H., Kurokawa H., Kawano H., Ando R., Shimogori T., Noda H., Fukami K., Sakaue-Sawano A., Miyawaki A. Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nat. Neurosci. 2011, 14:1481-1488.
17. Dufau I., Frongia C., Sicard F., Dedieu L., Cordelier P., Ausseil F., Ducommun B., Valette A. Multicellular tumor spheroid model to evaluate spatio-temporal dynamics effect of chemotherapeutics: application to the gemcitabine/CHK1 inhibitor combination in pancreatic cancer. BMC Cancer 2012, 12:15.