Comparison of Flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences

Journal of Clinical Microbiology

Volumn 39, Issue 5, 2001, Pages 1922-1927

  Scaramozzino, N ^a   Crance, J M ^a   Jouan, A ^a   DeBriel, D A ^a   Stoll, F ^a   Garin, D ^a  

^a CENTRE DE RECHERCHES   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR GEL ELECTROPHORESIS; ARBOVIRUS; ARTICLE; CEREBROSPINAL FLUID ANALYSIS; ENCEPHALITIS; GENE AMPLIFICATION; GENE SEQUENCE; MORBIDITY; MORTALITY; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE; STRAIN DIFFERENCE; TECHNIQUE; VIRUS TRANSMISSION; WEST NILE FLAVIVIRUS;

ANIMALS; BASE SEQUENCE; CONSERVED SEQUENCE; DNA PRIMERS; FLAVIVIRUS; FLAVIVIRUS INFECTIONS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; VIRAL NONSTRUCTURAL PROTEINS;

EID: 0035021876     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.39.5.1922-1927.2001     Document Type: Article

References (30)

1. Isolation of West Nile Virus from mosquitoes, crows and a cooper's hawk in Connecticut
2. Outbreak of West Nile-like viral encephalitis: New York
3. Extensive nucleotide changes and deletions within the envelope glycoprotein gene of Euro-African West Nile viruses
4. Identification of a Kunjin/West Nile-like flavivirus in brains of patients with New York encephalitis
5. Guidelines for surveillance, prevention and control of West Nile Virus infection - United States
6. Flavivirus genome organisation, expression, and replication
7. An integrated target sequence and signal amplification assay, reverse transcriptase-PCR-enzyme-linked immunosorbent assay, to detect and characterize flaviviruses
8. Use of NS3 consensus primers for the polymerase chain reaction amplification and sequencing of dengue viruses and other flaviviruses
9. PCR for diagnosis of viral infections of the central nervous system
10. Inhibition of Sandfly Fever Sicilian virus (Phlebovirus) replication in vitro by antiviral compounds
11. Molecular detection and characterisation of Yellow fever virus in blood and liver specimens of a non-vaccinated fatal human case
12. Detection of flavivirus by reverse-transcriptase polymerase chain reaction
13. Groups face to sequence and identify New York virus
14. New York's lethal virus came from Middle East, DNA suggests
15. Rapid identification of flaviviruses based on conserved NS5 gene sequences
16. Clustal: A package for performing multiple sequence alignments on a microcomputer
17. Cloning and sequence analysis of the genes encoding the non-structural proteins of Langat Virus and comparative analysis with other flaviviruses
18. Genetic analysis of West Nile New York 1999 encephalitis virus
19. Universal diagnostic RT-PCR protocol for arboviruses
20. Phylogeny of the genus Flavivirus
21. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcription-polymerase chain reaction
22. Origin of the West Nile Virus responsible for ah outbreak of encephalitis in the Northeastern United States
23. Rapid and sensitive protein similarity searches
24. Detection of flaviviruses by reverse transcriptase-polymerase chain reaction with the universal primer set
25. Flaviviruses
26. Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction
27. Detection of viral RNA by microplate hybridisation
28. Rapid identification of Flavivirus using the polymerase chain reaction
29. Population dynamics of flaviviruses revealed by molecular phylogenies