Inhibition of Mycobacterium tuberculosis PknG by non-catalytic rubredoxin domain specific modification: Reaction of an electrophilic nitro-fatty acid with the Fe-S center

Free Radical Biology and Medicine

Volumn 65, Issue , 2013, Pages 150-161

  Gil, Magdalena ^a,b   Graña, Martín ^a   Schopfer, Francisco J ^c   Wagner, Tristan ^d,f   Denicola, Ana ^e   Freeman, Bruce A ^c   Alzari, Pedro M ^d   Batthyány, Carlos ^a,e   Durán, Rosario ^a,b  

^a INSTITUT PASTEUR DE MONTEVIDEO   (Uruguay)

^b INSTITUTO DE INVESTIGACIONES BIOLÓGICAS CLEMENTE ESTABLE   (Uruguay)

^c UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^d INSTITUT PASTEUR   (France)

^e UNIVERSIDAD DE LA REPÚBLICA   (Uruguay)

^f MAX PLANCK INSTITUTE FOR TERRESTRIAL MICROBIOLOGY   (Germany)

Author keywords

Mycobacterium tuberculosis; Nitratedfattyacids; Nitroalkene; PknG; Rubredoxin; Ser Thr kinase

Indexed keywords

BACTERIAL PROTEIN; CYSTEINE; FATTY ACID; IRON; PKNG PROTEIN; PROTEIN SERINE THREONINE KINASE; RUBREDOXIN; SULFUR; UNCLASSIFIED DRUG; ALKENE; NITRO DERIVATIVE; PKNH PROTEIN, MYCOBACTERIUM TUBERCULOSIS;

ALKYLATION; ARTICLE; CELLULAR DISTRIBUTION; CIRCULAR DICHROISM; CONTROLLED STUDY; ELECTROPHILICITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME STRUCTURE; MASS SPECTROMETRY; METAL BINDING; MICHAEL ADDITION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NUCLEOPHILICITY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; REACTION TIME; CHEMISTRY; METABOLISM; PHYSIOLOGY; SITE DIRECTED MUTAGENESIS;

ALKENES; BACTERIAL PROTEINS; CATALYTIC DOMAIN; CIRCULAR DICHROISM; FATTY ACIDS; MUTAGENESIS, SITE-DIRECTED; MYCOBACTERIUM TUBERCULOSIS; NITRO COMPOUNDS; PROTEIN-SERINE-THREONINE KINASES; RUBREDOXINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84884569103     PISSN: 08915849     EISSN: 18734596     Source Type: Journal    
DOI: 10.1016/j.freeradbiomed.2013.06.021     Document Type: Article

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