A thermostable exo-β-fructosidase immobilised through rational design

Food Chemistry

Volumn 145, Issue , 2014, Pages 826-831

  Martínez, Duniesky ^a   Cutiño Avila, Bessy ^b   Pérez, Enrique Rosendo ^a   Menéndez, Carmen ^c   Hernández, Lázaro ^c   Del Monte Martínez, Alberto ^b  

^a Fermentation Laboratory   (Cuba)

^b UNIVERSITY OF HAVANA   (Cuba)

^c CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Covalent immobilization; Invert syrup; Invertase; Rational design

Indexed keywords

BATCH STIRRED TANK REACTORS; CATALYTIC PROPERTIES; COVALENT IMMOBILIZATION; HIGH REACTION TEMPERATURES; INDUSTRIAL CONDITIONS; INVERT SYRUP; INVERTASE; RATIONAL DESIGN;

ENZYME ACTIVITY;

DESIGN;

BETA FRUCTOFURANOSIDASE; EXO BETA FRUCTOSIDASE; SUCROSE; UNCLASSIFIED DRUG;

ARTICLE; BATCH REACTOR; BIOCATALYST; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME MECHANISM; ENZYME SUBSTRATE; FIXED BED REACTOR; HALF LIFE TIME; HYDROLYSIS; STIRRED REACTOR; THERMOSTABILITY;

PICHIA PASTORIS; THERMOTOGA MARITIMA;

COVALENT IMMOBILISATION; INVERT SYRUP; INVERTASE; RATIONAL DESIGN;

BACTERIAL PROTEINS; BIOCATALYSIS; CHROMATOGRAPHY, AGAROSE; COMPUTATIONAL BIOLOGY; DIETARY SUCROSE; ENZYME STABILITY; ENZYMES, IMMOBILIZED; EXPERT SYSTEMS; FOOD HANDLING; FRUCTOSE; GLUCOSE; GLYCOSIDE HYDROLASES; GLYCOSYLATION; GLYOXYLATES; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MODELS, MOLECULAR; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEPHAROSE; THERMOTOGA MARITIMA;

EID: 84884484332     PISSN: 03088146     EISSN: 18737072     Source Type: Journal    
DOI: 10.1016/j.foodchem.2013.08.073     Document Type: Article

References (24)

1. F. Alberto, E. Jordi, B. Henrissat, and M. Czjzek Crystal structure of inactivated Thermotoga maritima invertase in complex with the trisaccharide substrate raffinose Biochemical Journal 395 2006 457 462
2. L. Amaya-Delgado, M.E. Hidalgo-Lara, and M.C. Montes-Horcasitas Hydrolysis of sucrose by invertase immobilized on nylon-6 microbeads Food Chemistry 99 2006 299 304
3. U. Andjelković, S. Pićurić, and Z. Vujčić Purification and characterisation of Saccharomyces cerevisiae external invertase isoforms Food Chemistry 120 2010 799 804
4. R. Bergamasco, F.J. Bassetti, F. Moraes, and G.M. Zanin Characterization free and immobilized invertase regarding activity and energy of activation Brazilian Journal of Chemical Engineering 17 2000 4 7
5. M.M. Bradford A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding Analytical Biochemistry 72 1976 248 254
6. J.M. Cregg, J.F. Tschopp, C. Stillman, R. Siegel, M. Akong, and W.S. Craig High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris Nature Biotechnology 5 1987 479 485 (Pubitemid 17081272)
7. T. Danisman, S. Tan, Y. Kacar, and A. Ergene Covalent immobilization of invertase on microporous pHEMA-GMA membrane Food Chemistry 85 2004 461 466 (Pubitemid 38064202)
8. A. del Monte, H. Nolasco, A. Forrellat, C. Aragón, A. García, and J. Díaz Evidencias de la presencia de lipasas en el hepatopáncreas de Litopenaeus schmitii Revista Electrónica AquaTIC 6 2002 207 222
9. A. del Monte-Martínez, and B.V. Cutiño-Avila Rational design of immobilized lipases and phospholipases Methods in Molecular Biology 861 2012 343 382
10. DeLano, W. L. (2002). PyMOL Reference Guide. In DeLano Scientific LLC (Eds.), The PyMOL molecular graphics system (pp. 1-68). San Carlos, California, Palo Alto, CA, USA. http://www.pymol.org.
11. E. Emregül, S. Sungur, and U. Akbulut Polyacrylamide-gelatine carrier system used for invertase immobilization Food Chemistry 97 2006 591 597 (Pubitemid 43181655)
12. J.M. Guisán Aldehyde-agarose gels as activated supports for immobilisation-stabilization of enzymes Enzyme and Microbial Technology 10 1988 375 382
13. J.M. Guisán, R. Fernández-Lafuente, V. Rodríguez, A. Bastida, R.M. Blanco, and G. Alvaro Enzyme stabilization by multipoint covalent attachment to activated pre-existing supports W.J.J. Van del Tweel, A. Harder, R.M. Buitelaar, Stability stabilization of enzymes 1993 Elsevier Science Publisher B.V Amsterdam 55 62
14. P. Hasal, V. Vojtisek, A. Cejkova, P. Kleezek, and O. Kofronova An immobilized whole yeast cell biocatalyst for enzymatic sucrose hydrolysis Enzyme and Microbial Technology 14 1992 221 229
15. S.M. Kotwal, and V. Shankar Immobilized invertase Biotechnology Advances 27 2009 311 322
16. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
17. W. Liebl, D. Brem, and A. Gotschlich Analysis of the gene for β-fructosidase (invertase, inulinase) of the hyperthermophilic bacterium Thermotoga maritima, and characterisation of the enzyme expressed in Escherichia coli Applied Microbiology and Biotechnology 50 1998 55 64 (Pubitemid 28372376)
18. C. Mateo, J.M. Palomo, M. Fuentes, L. Betancor, V. Grazu, and F. López-Gallego Glyoxyl-agarose: a fully inert hydrophilic support for immobilization and high stabilization of proteins Enzyme and Microbial Technology 39 2006 274 280
19. C. Menéndez, D. Martínez, L.E. Trujillo, Y. Mazola, E. González, and E.R. Pérez Constitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris Applied Microbiology and Biotechnology 97 2013 1201 1212
20. G.L. Miller Use of dinitrosalicylic acid reagent for determination of reducing sugar Analytical Chemistry 31 1959 426 428
21. A. Milovanović, N. Bozic, and Z. Vujčić Cell wall invertase immobilization within calcium alginate beads Food Chemistry 104 2007 81 86 (Pubitemid 46562546)
22. I. Muñoz-Gutiérrez, M.E. Rodríguez-Alegría, and A. López-Mungía Kinetic behavior and specificity of β-fructosidases in the hydrolysis of plant and microbial fructans Process Biochemistry 44 2009 891 898
23. J. Pedroche, M.M. Yust, C. Mateo, R. Fernández-Lafuente, J. Girón-Calle, and M. Alaiz Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: correlation between enzyme-support linkages and thermal stability Enzyme and Microbial Technology 40 2007 1160 1166 (Pubitemid 46356525)
24. R. Prodanovic, M. Simic, and Z. Vujčić Immobilization of periodate oxidized invertase by adsorption on sepiolite Journal of the Serbian Chemical Society 11 2003 819 824 (Pubitemid 38281361)