A versatile mini-mazF-cassette for marker-free targeted genetic modification in Bacillus subtilis

Journal of Microbiological Methods

Volumn 95, Issue 2, 2013, Pages 207-214

  Lin, Zhiwei ^a   Deng, Bin ^a   Jiao, Zhihua ^a,b   Wu, Bingbing ^a   Xu, Xin ^a   Yu, Dongyou ^a   Li, Weifen ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

Bacillus subtilis; Fusion PCR; Marker free; MazF; Mini mazF cassette

Indexed keywords

AMYE GENE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL STRAIN; CELL VIABILITY; CONTROLLED STUDY; DNA FRAGMENTATION; ENZYME ACTIVITY; GENE CASSETTE; GENE CLUSTER; GENE DISRUPTION; GENE LOCUS; HOMOLOGOUS RECOMBINATION; MAZF GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN SYNTHESIS; RESTRICTION SITE; SEQUENCE HOMOLOGY; TRANSCRIPTION REGULATION;

BACILLUS SUBTILIS; FUSION PCR; MARKER-FREE; MAZF; MINI-MAZF-CASSETTE;

BACILLUS SUBTILIS; BLEOMYCIN; DRUG RESISTANCE, BACTERIAL; GENE DELETION; GENE KNOCK-IN TECHNIQUES; GENE KNOCKOUT TECHNIQUES; GENETIC ENGINEERING; GENETIC MARKERS; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; PLASMIDS; PROMOTER REGIONS, GENETIC; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA; SEQUENCE DELETION;

EID: 84884369721     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.07.020     Document Type: Article

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