p-FAK-Tyr397 regulates spermatid adhesion in the rat testis via its effects on F-actin organization at the ectoplasmic specialization

American Journal of Physiology - Endocrinology and Metabolism

Volumn 305, Issue 6, 2013, Pages

  Wan, Hin Ting ^a   Mruk, Dolores D ^a   Li, Stephen Y T ^a   Mok, Ka Wai ^a   Lee, Will M ^b   Wong, Chris K C ^c   Cheng, C Yan ^a  

^a POPULATION COUNCIL   (United States)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

^c HONG KONG BAPTIST UNIVERSITY   (Hong Kong)

Author keywords

Actin filament bundles; Adherens junction; Focal adhesion kinase; Focal adhesion kinase mutant; Spermatogenesis; Testis

Indexed keywords

ACTIN RELATED PROTEIN 3; EPIDERMAL GROWTH FACTOR RECEPTOR; F ACTIN; FOCAL ADHESION KINASE; NECTIN 2; P FOCAL ADHESION KINASE TYR 397; PHALLOIDIN; UNCLASSIFIED DRUG;

ACTIN FILAMENT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ADHESION; CELL SPECIFICITY; CELL TRANSPORT; CONTROLLED STUDY; FLUORESCENCE; GENETIC TRANSFECTION; IN VIVO STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; SEMINIFEROUS TUBULE EPITHELIUM; SERTOLI CELL; SPERMATID; TESTIS; UPREGULATION;

ACTIN FILAMENT BUNDLES; ADHERENS JUNCTION; FOCAL ADHESION KINASE; FOCAL ADHESION KINASE MUTANT; SPERMATOGENESIS; TESTIS;

ACTIN CYTOSKELETON; ACTINS; ANIMALS; ANIMALS, GENETICALLY MODIFIED; BLOOD-TESTIS BARRIER; CELL ADHESION; FOCAL ADHESION KINASE 1; MALE; PHOSPHORYLATION; RATS; RATS, SPRAGUE-DAWLEY; SEMINIFEROUS EPITHELIUM; SERTOLI CELLS; SPERMATIDS; SPERMATOGENESIS; TESTIS; TYROSINE;

EID: 84884359691     PISSN: 01931849     EISSN: 15221555     Source Type: Journal    
DOI: 10.1152/ajpendo.00254.2013     Document Type: Article

References (40)

1. Anitei M, Hoflack B. Bridging membrane and cytoskeleton dynamics in the secretory and endocytic pathways. Nat Cell Biol 14: 11-19, 2011.
2. Beardsley A, Robertson DM, O'Donnell L. A complex containing α6β1-integrin and phosphorylated focal adhesion kinase between Sertoli cells and elongated spermatids during spermatid release from the seminiferous epithelium. J Endocrinol 190: 759-770, 2006.
3. Boutros T, Chevet E, Metrakos P. Mitogen-activated protein (MAP) kinase/MAP kinase phosphatase regulation: roles in cell growth, death, and cancer. Pharmacol Rev 60: 261-310, 2008.
4. Cheng CY, Mruk DD. The blood-testis barrier and its implication in male contraception. Pharmacol Rev 64: 16-64, 2012.
5. Cheng CY, Mruk DD. An intracellular trafficking pathway in the seminiferous epithelium regulating spermatogenesis: a biochemical and molecular perspective. Crit Rev Biochem Mol Biol 44: 245-263, 2009.
6. Cheng CY, Mruk DD. A local autocrine axis in the testes that regulates spermatogenesis. Nat Rev Endocrinol 6: 380-395, 2010.
7. Cheng CY, Mruk DD. Regulation of blood-testis barrier dynamics by focal adhesion kinase (FAK). An unexpected turn of events. Cell Cycle 8: 3493-3499, 2009.
8. de Kretser DM, Kerr JB. The cytology of the testis. In: The Physiology of Reproduction Vol 1, edited by Knobil E, Neill JB, Ewing LL, Greenwald GS, Markert CL, and Pfaff DW. New York, NY: Raven, 1988, p. 837-932.
9. Grima J, Zhu LJ, Zong SD, Catterall JF, Bardin CW, Cheng CY. Rat testin is a newly identified component of the junctional complexes in various tissues whose mRNA is predominantly expressed in the testis and ovary. Biol Reprod 52: 340-355, 1995.
10. Hess RA, de Franca LR. Spermatogenesis and cycle of the seminiferous epithelium. Adv Exp Med Biol 636: 1-15, 2008.
11. Inagaki M, Irie K, Ishizaki H, Tanaka-Okamoto M, Miyoshi J, Takai Y. Role of cell adhesion molecule nectin-3 in spermatid development. Genes Cells 11: 1125-1132, 2006.
12. Lie PPY, Chan AYN, Mruk DD, Lee WM, Cheng CY. Restricted Arp3 expression in the testis prevents blood-testis barrier disruption during junction restructuring at spermatogenesis. Proc Natl Acad Sci USA 107: 11411-11416, 2010.
13. Lie PPY, Mruk DD, Lee WM, Cheng CY. Epidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epithelium. FASEB J 23: 2555-2567, 2009.
14. Lie PPY, Mruk DD, Mok KW, Su L, Lee WM, Cheng CY. Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on bloodtestis barrier dynamics in the rat. Proc Natl Acad Sci USA 109: 12562-12567, 2012.
15. Mok KW, Mruk DD, Silvestrini B, Cheng CY. rpS6 regulates bloodtestis barrier dynamics by affecting F-actin organization and protein recruitment. Endocrinology 153: 5036-5048, 2012.
16. Mruk DD, Cheng CY. Cell-cell interactions at the ectoplasmic specialization in the testis. Trends Endocrinol Metab 15: 439-447, 2004.
17. Mruk DD, Cheng CY. Enhanced chemiluminescence (ECL) for routine immunoblotting. An inexpensive alternative to commercially available kits. Spermatogenesis 1: 121-122, 2011.
18. Mruk DD, Cheng CY. An in vitro system to study Sertoli cell blood-testis barrier dynamics. Methods Mol Biol 763: 237-252, 2011.
19. Mruk DD, Cheng CY. Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis. Endocr Rev 25: 747-806, 2004.
20. Mruk DD, Silvestrini B, Cheng CY. Anchoring junctions as drug targets: role in contraceptive development. Pharmacol Rev 60: 146-180, 2008.
21. Mruk DD, Wong CH, Silvestrini B, Cheng CY. A male contraceptive targeting germ cell adhesion. Nature Med 12: 1323-1328, 2006.
22. O'Donnell L, Nicholls PK, O'Bryan MK, McLachlan RI, Stanton PG. Spermiation: the process of sperm release. Spermatogenesis 1: 14-35, 2011.
23. Ozaki-Kuroda K, Nakanishi H, Ohta H, Tanaka H, Kurihara H, Mueller S, Irie K, Ikeda W, Sakai T, Wimmer E, Nishimune Y, Takai Y. Nectin couples cell-cell adhesion and the actin scaffold at heterotypic testicular junctions. Curr Biol 12: 1145-1150, 2002.
24. Parvinen M. Regulation of the seminiferous epithelium. Endocr Rev 3: 404-417, 1982.
25. Qian X, Mruk DD, Wong EWP, Lie PPY, Cheng CY. Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in the rat testis. Endocrinology 154: 1907-1920, 2013.
26. Russell LD. Further observations on tubulobulbar complexes formed by late spermatids and Sertoli cells in the rat testis. Anat Rec 194: 213-232, 1979.
27. Russell LD. Observations on rat Sertoli ectoplasmic ('junctional') specializations in their association with germ cells of the rat testis. Tissue Cell 9: 475-498, 1977.
28. Russell LD, Saxena NK, Weber JE. Intratesticular injection as a method to assess the potential toxicity of various agents to study mechanisms of normal spermatogenesis. Gamete Res 17: 43-56, 1987.
29. Siu MKY, Mruk DD, Lee WM, Cheng CY. Adhering junction dynamics in the testis are regulated by an interplay of β1-integrin and focal adhesion complex (FAC)-associated proteins. Endocrinology 144: 2141-2163, 2003.
30. Su L, Mruk DD, Lie PPY, Silvestrini B, Cheng CY. A peptide derived from laminin- 3 reversibly impairs spermatogenesis in rats. Nat Communs 3: 1185 (doi:1110.1038/ncomms2171), 2012.
31. Vallejo AN, Pogulis RJ, Pease LR. PCR mutagenesis by overlap extension and gene SOE. CSH Protoc 2008: pdb.prot4861, 2008.
32. Vogl AW, Vaid KS, Guttman JA. The Sertoli cell cytoskeleton. Adv Exp Med Biol 636: 186-211, 2008.
33. Weber JE, Russell LD, Wong V, Peterson RN. Three dimensional reconstruction of a rat stage V Sertoli cell. II. Morphometry of Sertoli- Sertoli and Sertoli-germ cell relationships. Am J Anat 167: 163-179, 1983.
34. Wolski KM, Perrault C, Tran-Son-Tay R, Cameron DF. Strength measurement of the Sertoli-spermatid junctional complex. J Androl 26: 354-359, 2005.
35. Wong EWP, Cheng CY. Polarity proteins and cell-cell interactions in the testis. Int Rev Cell Mol Biol 278: 309-353, 2009.
36. Wong EWP, Mruk DD, Cheng CY. Biology and regulation of ectoplasmic specialization, an atypical adherens junction type, in the testis. Biochem Biophys Acta 1778: 692-708, 2008.
37. Wong EWP, Mruk DD, Lee WM, Cheng CY. Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis. Proc Natl Acad Sci USA 105: 9657-9662, 2008.
38. Wong EWP, Mruk DD, Lee WM, Cheng CY. Regulation of blood-testis barrier dynamics by TGF-_3 is a Cdc42-dependent protein trafficking event. Proc Natl Acad Sci USA 107: 11399-11404, 2010.
39. Young JS, Guttman JA, Vaid KS, Vogl AW. Cortactin (CTTN), NWASP (WASL), clathrin (CLTC) are present at podosome-like tubulobulbar complexes in the rat testis. Biol Reprod 80: 153-161, 2009.
40. Young JS, Vogl AW. Focal adhesion proteins zyxin and vinculin are co-distributed at tubulobulbar complexes. Spermatogenesis 2: 63-68, 2012.