Restricted Arp3 expression in the testis prevents blood-testis barrier disruption during junction restructuring at spermatogenesis

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 25, 2010, Pages 11411-11416

  Lie, Pearl P Y ^a   Chan, Apple Y N ^a   Mruk, Dolores D ^a   Lee, Will M ^b   Cheng, C Yan ^a  

^a POPULATION COUNCIL   (United States)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Actin dynamics; Ectoplasmic specialization; Seminiferous epithelial cycle; Spermiogenesis

Indexed keywords

ACTIN; ACTIN RELATED PROTEIN 2; ACTIN RELATED PROTEIN 3; NEURAL WISKOTT ALDRICH SYNDROME PROTEIN; PROTEIN INHIBITOR; TESTOSTERONE; UNCLASSIFIED DRUG; WISKOSTATIN; CARBAZOLE DERIVATIVE; N WASP PROTEIN, RAT; N-WASP PROTEIN, RAT; PROPANOLAMINE DERIVATIVE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLOOD TESTIS BARRIER; CONTROLLED STUDY; MALE; MEMBRANE STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; SEMINIFEROUS TUBULE; SIGNAL TRANSDUCTION; SPERMATID; SPERMATOCYTE; SPERMATOGENESIS; SPERMATOZOON MOTILITY; TIGHT JUNCTION; ANIMAL; CELL COMMUNICATION; CELL NUCLEUS; CYTOPLASM; EPITHELIUM; METABOLISM; PATHOLOGY; SERTOLI CELL; SPRAGUE DAWLEY RAT;

ACTIN-RELATED PROTEIN 3; ANIMALS; BLOOD-TESTIS BARRIER; CARBAZOLES; CELL COMMUNICATION; CELL NUCLEUS; CYTOPLASM; EPITHELIUM; MALE; PROPANOLAMINES; RATS; RATS, SPRAGUE-DAWLEY; SERTOLI CELLS; SPERMATOGENESIS; TIGHT JUNCTIONS; WISKOTT-ALDRICH SYNDROME PROTEIN, NEURONAL;

EID: 77954920588     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1001823107     Document Type: Article

References (27)

1. Hartsock A, Nelson WJ (2008) Adherens and tight junctions: Structure, function and connections to the actin cytoskeleton. Biochim Biophys Acta 1778:660-669.
2. Wong EWP, Mruk DD, Cheng CY (2008) Biology and regulation of ectoplasmic specialization, an atypical adherens junction type, in the testis. Biochim Biophys Acta 1778:692-708.
3. Vogl A, Vaid K, Guttman J (2008) The Sertoli cell cytoskeleton. Molecular Mechanisms in Spermatogenesis, ed Cheng CY (Landes Bioscience/Springer Science+Business Media, Austin, TX), pp 186-211.
4. Goley ED, Welch MD (2006) The ARP2/3 complex: An actin nucleator comes of age. Nat Rev Mol Cell Biol 7:713-726.
5. Weaver AM, Young ME, Lee WL, Cooper JA (2003) Integration of signals to the Arp2/3 complex. Curr Opin Cell Biol 15:23-30.
6. D'Souza R, et al. (2009) Disruption of tubulobulbar complex by high intratesticular estrogens leading to failed spermiation. Endocrinology 150:1861-1869.
7. Young JS, Guttman JA, Vaid KS, Shahinian H, Vogl AW (2009) Cortactin (CTTN), NWASP (WASL), and clathrin (CLTC) are present at podosome-like tubulobulbar complexes in the rat testis. Biol Reprod 80:153-161.
8. Lie PPY, et al. (2006) Dynamin II interacts with the cadherin- and occludin-based protein complexes at the blood-testis barrier in adult rat testes. J Endocrinol 191:571-586.
9. Cheng CY, et al. (2005) AF-2364 [1-(2,4-dichlorobenzyl)-1H-indazole-3- carbohydrazide] is a potential male contraceptive: A review of recent data. Contraception 72:251-261.
10. Ivanov AI, Hunt DF, Utech M, Nusrat A, Parkos CA (2005) Differential roles for actin polymerization and a myosin II motor in assembly of the epithelial apical junctional complex. Mol Biol Cell 16:2636-2650.
11. Legg JA, et al. (2007) N-WASP involvement in dorsal ruffle formation in mouse embryonic fibroblasts. Mol Biol Cell 18:678-687.
12. Siu MKY, Wong CH, Lee WM, Cheng CY (2005) Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases. J Biol Chem 280:25029-25047.
13. Meng J, Holdcraft RW, Shima JE, Griswold MD, Braun RE (2005) Androgens regulate the permeability of the blood-testis barrier. Proc Natl Acad Sci USA 102:16696-16700.
14. Yan HHN, Mruk DD, Lee WM, Cheng CY (2008) Blood-testis barrier dynamics are regulated by testosterone and cytokines via their differential effects on the kinetics of protein endocytosis and recycling in Sertoli cells. FASEB J 22:1945-1959.
15. Derivery E, Gautreau A (2010) Generation of branched actin networks: Assembly and regulation of the N-WASP and WAVE molecular machines. Bioessays 32:119-131.
16. Peterson JR, et al. (2004) Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nat Struct Mol Biol 11:747-755.
17. Lie PPY, Mruk DD, Lee WM, Cheng CY (2009) Epidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epithelium. FASEB J 23:2555-2567.
18. Scita G, et al. (2001) An effector region in Eps8 is responsible for the activation of the Rac-specific GEF activity of Sos-1 and for the proper localization of the Rac-based actin-polymerizing machine. J Cell Biol 154:1031-1044.
19. Disanza A, et al. (2004) Eps8 controls actin-based motility by capping the barbed ends of actin filaments. Nat Cell Biol 6:1180-1188.
20. Disanza A, et al. (2006) Regulation of cell shape by Cdc42 is mediated by the synergic actin-bundling activity of the Eps8-IRSp53 complex. Nat Cell Biol 8:1337-1347.
21. Drees F, Pokutta S, Yamada S, Nelson WJ, Weis WI (2005) α-catenin is a molecular switch that binds E-cadherin-β-catenin and regulates actin-filament assembly. Cell 123:903-915.
22. Ridley AJ, et al. (2003) Cell migration: Integrating signals from front to back. Science 302:1704-1709.
23. Mruk DD, Silvestrini B, Cheng CY (2008) Anchoring junctions as drug targets: Role in contraceptive development. Pharmacol Rev 60:146-180.
24. Young JS, Guttman JA, Vaid KS, Vogl AW (2009) Tubulobulbar complexes are intercellular podosome-like structures that internalize intact intercellular junctions during epithelial remodeling events in the rat testis. Biol Reprod 80:162-174.
25. Wong EWP, Mruk DD, Lee WM, Cheng CY (2008) Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis. Proc Natl Acad Sci USA 105:9657-9662.
26. Wong EWP, Sun S, Li MWM, Lee WM, Cheng CY (2009) 14-3-3 Protein regulates cell adhesion in the seminiferous epithelium of rat testes. Endocrinology 150:4713-4723.
27. Wang RS, et al. (2006) Androgen receptor in sertoli cell is essential for germ cell nursery and junctional complex formation in mouse testes. Endocrinology 147:5624-5633.