Development of Monoclonal Antibodies and Immunoassays for Sensitive and Specific Detection of Shiga Toxin Stx2f

PLoS ONE

Volumn 8, Issue 9, 2013, Pages

  Skinner, Craig ^a   Patfield, Stephanie ^a   Stanker, Larry ^a   He, Xiaohua ^a  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MONOCLONAL ANTIBODY; VEROTOXIN 1; VEROTOXIN 2; VIRULENCE FACTOR;

ARTICLE; BACTERIAL STRAIN; BINDING ASSAY; CROSS REACTION; ENZYME IMMUNOASSAY; ESCHERICHIA COLI; IMMUNOASSAY; IMMUNOBLOTTING; LIMIT OF DETECTION; NONHUMAN; PURIFICATION; SENSITIVITY AND SPECIFICITY; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, NEUTRALIZING; ANTIBODY SPECIFICITY; CELL LINE; CHICKENS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMANS; IMMUNOASSAY; MICE; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN 2;

EID: 84884268685     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0076563     Document Type: Article

References (45)

1. Centers for Disease Control and Prevention (CDC) (2013) Surveillance for foodborne disease outbreaks- United States, 2009-2010. MMWR Morb Mortal Wkly Rep 62: 41-47. PubMed: 23344696.
2. Gerner-Smidt P, Hise K, Kincaid J, Hunter S, Rolando S, et al. (2006) PulseNet USA: a five-year update. Foodborne Pathog Dis 3: 9-19. doi:10.1089/fpd.2006.3.9. PubMed: 16602975.
3. Hussein HS, (2007) Prevalence and pathogenicity of Shiga toxin-producing Escherichia coli in beef cattle and their products. J Anim Sci 85: E63-E72. doi:10.2527/jas.2006-421. PubMed: 17060419.
4. Cantey JR, (1985) Shiga toxin--an expanding role in the pathogenesis of infectious diseases. J Infect Dis 151: 766-771. doi:10.1093/infdis/151.5.766. PubMed: 3886803.
5. Pai CH, Kelly JK, Meyers GL, (1986) Experimental infection of infant rabbits with verotoxin-producing Escherichia coli. Infect Immun 51: 16-23. PubMed: 3510166.
6. Feng PC, Jinneman K, Scheutz F, Monday SR, (2011) Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes. Appl Environ Microbiol 77: 6699-6702. doi:10.1128/AEM.00370-11. PubMed: 21803918.
7. Mellmann A, Harmsen D, Cummings CA, Zentz EB, Leopold SR, et al. (2011) Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology. PLOS ONE 6: e22751. doi:10.1371/journal.pone.0022751. PubMed: 21799941.
8. Bielaszewska M, Mellmann A, Zhang W, Köck R, Fruth A, et al. (2011) Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. Lancet Infect Dis 11: 671-676. PubMed: 21703928.
9. Brzuszkiewicz E, Thürmer A, Schuldes J, Leimbach A, Liesegang H, et al. (2011) Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC). Arch Microbiol 193: 883-891. doi:10.1007/s00203-011-0725-6. PubMed: 21713444.
10. Delannoy S, Beutin L, Burgos Y, Fach P, (2012) Specific detection of enteroaggregative hemorrhagic Escherichia coli O104:H4 strains by use of the CRISPR locus as a target for a diagnostic real-time PCR. J Clin Microbiol 50: 3485-3492. doi:10.1128/JCM.01656-12. PubMed: 22895033.
11. Kalchayanand N, Arthur TM, Bosilevac JM, Schmidt JW, Wang R, et al. (2012) Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7. J Food Protect 75: 1207-1212. doi:10.4315/0362-028X.JFP-11-531. PubMed: 22980002.
12. Frank C, Werber D, Cramer JP, Askar M, Faber M, et al. (2011) Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in Germany. N Engl J Med 365: 1771-1780. doi:10.1056/NEJMoa1106483. PubMed: 21696328.
13. Gamage SD, Patton AK, Hanson JF, Weiss AA, (2004) Diversity and host range of Shiga toxin-encoding phage. Infect Immun 72: 7131-7139. doi:10.1128/IAI.72.12.7131-7139.2004. PubMed: 15557637.
14. Ooka T, Seto K, Kawano K, Kobayashi H, Etoh Y, et al. (2012) Clinical significance of Escherichia albertii. Emerg Infect Dis 18: 488-492. doi:10.3201/eid1803.111401. PubMed: 22377117.
15. Paton AW, Paton JC, (1996) Enterobacter cloacae producing a Shiga-like toxin II-related cytotoxin associated with a case of hemolytic-uremic syndrome. J Clin Microbiol 34: 463-465. PubMed: 8789041.
16. Samuel JE, Perera LP, Ward S, O'Brien AD, Ginsburg V, et al. (1990) Comparison of the glycolipid receptor specificities of Shiga-like toxin type II and Shiga-like toxin type II variants. Infect Immun 58: 611-618. PubMed: 2155181.
17. Endo Y, Tsurugi K, Yutsudo T, Takeda Y, Ogasawara T, et al. (1988) Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N-glycosidase activity of the toxins. Eur J Biochem 171: 45-50. doi:10.1111/j.1432-1033.1988.tb13756.x. PubMed: 3276522.
18. Fuller CA, Pellino CA, Flagler MJ, Strasser JE, Weiss AA, (2011) Shiga toxin subtypes display dramatic differences in potency. Infect Immun 79: 1329-1337. doi:10.1128/IAI.01182-10. PubMed: 21199911.
19. Tesh VL, Burris JA, Owens JW, Gordon VM, Wadolkowski EA, et al. (1993) Comparison of the relative toxicities of Shiga-like toxins type I and type II for mice. Infect Immun 61: 3392-3402. PubMed: 8335369.
20. Oloomi M, Bouzari S, Arshadi M, (2006) N-terminus leader sequence of Shiga toxin (Stx) 1 is essential for production of active recombinant protein in E. coli. Protein Pept Lett 13: 509-512. doi:10.2174/092986606776819538. PubMed: 16800807.
21. McGannon CM, Fuller CA, Weiss AA, (2010) Different classes of antibiotics differentially influence shiga toxin production. Antimicrob Agents Chemother 54: 3790-3798. doi:10.1128/AAC.01783-09. PubMed: 20585113.
22. Wagner PL, Livny J, Neely MN, Acheson DW, Friedman DI, et al. (2002) Bacteriophage control of Shiga toxin 1 production and release by Escherichia coli. Mol Microbiol 44: 957-970. doi:10.1046/j.1365-2958.2002.02950.x. PubMed: 12010491.
23. Law D, Ganguli LA, Donohue-Rolfe A, Acheson DW, (1992) Detection by ELISA of low numbers of Shiga-like toxin-producing Escherichia coli in mixed cultures after growth in the presence of mitomycin C. J Med Microbiol 36: 198-202. doi:10.1099/00222615-36-3-198. PubMed: 1548693.
24. Zhang X, McDaniel AD, Wolf LE, Keusch GT, Waldor MK, et al. (2000) Quinolone antibiotics induce Shiga toxin-encoding bacteriophages, toxin production, and death in mice. J Infect Dis 181: 664-670. doi:10.1086/315239. PubMed: 10669353.
25. Hertig A, Ridel C, Rondeau E, (2010) [Hemolytic uremic syndrome in adults]. Nephrol Ther 6: 258-271. doi:10.1016/j.nephro.2010.03.002. PubMed: 20399168.
26. Menne J, Nitschke M, Stingele R, Abu-Tair M, Beneke J, et al. (2012) Validation of treatment strategies for enterohaemorrhagic Escherichia coli O104:H4 induced haemolytic uraemic syndrome: case-control study. Bmj 345: e4565. doi:10.1136/bmj.e4565. PubMed: 22815429.
27. Scheutz F, Teel LD, Beutin L, Piérard D, Buvens G, et al. (2012) Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature. J Clin Microbiol 50: 2951-2963. doi:10.1128/JCM.00860-12. PubMed: 22760050.
28. Orth D, Grif K, Khan AB, Naim A, Dierich MP, et al. (2007) The Shiga toxin genotype rather than the amount of Shiga toxin or the cytotoxicity of Shiga toxin in vitro correlates with the appearance of the hemolytic uremic syndrome. Diagn Microbiol Infect Dis 59: 235-242. doi:10.1016/j.diagmicrobio.2007.04.013. PubMed: 17931818.
29. Oanh TK, Nguyen VK, De Greve H, Goddeeris BM, (2012) Protection of piglets against Edema disease by maternal immunization with Stx2e toxoid. Infect Immun 80: 469-473. doi:10.1128/IAI.05539-11. PubMed: 22083704.
30. Schmidt H, Scheef J, Morabito S, Caprioli A, Wieler LH, et al. (2000) A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons. Appl Environ Microbiol 66: 1205-1208. doi:10.1128/AEM.66.3.1205-1208.2000. PubMed: 10698793.
31. Müthing J, Meisen I, Zhang W, Bielaszewska M, Mormann M, et al. (2012) Promiscuous Shiga toxin 2e and its intimate relationship to Forssman. Glycobiology 22: 849-862. doi:10.1093/glycob/cws009. PubMed: 22279060.
32. Tyrrell GJ, Ramotar K, Toye B, Boyd B, Lingwood CA, et al. (1992) Alteration of the carbohydrate binding specificity of verotoxins from Gal alpha 1-4Gal to GalNAc beta 1-3Gal alpha 1-4Gal and vice versa by site-directed mutagenesis of the binding subunit. Proc Natl Acad Sci U S A 89: 524-528. doi:10.1073/pnas.89.2.524. PubMed: 1731324.
33. Skinner C, McMahon S, Rasooly R, Carter JM, He X, (2013) Purification and characterization of shiga toxin 2f, an immunologically unrelated subtype of shiga toxin 2. PLOS ONE 8: e59760. doi:10.1371/journal.pone.0059760. PubMed: 23555772.
34. Willford J, Mills K, Goodridge LD, (2009) Evaluation of three commercially available enzyme-linked immunosorbent assay kits for detection of Shiga toxin. J Food Protect 72: 741-747. PubMed: 19435221.
35. Sugasawara RJ, Cahoon BE, Karu AE, (1985) The influence of murine macrophage-conditioned medium on cloning efficiency, antibody synthesis, and growth rate of hybridomas. J Immunol Methods 79: 263-275. doi:10.1016/0022-1759(85)90106-1. PubMed: 3998479.
36. He X, McMahon S, Skinner C, Merrill P, Scotcher MC, et al. (2013) Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. J Immunol Methods, 389: 18-28. PubMed: 23279946.
37. Momtaz H, Jamshidi A, (2013) Shiga toxin-producing Escherichia coli isolated from chicken meat in Iran: Serogroups, virulence factors, and antimicrobial resistance properties. Poult Sci 92: 1305-1313. doi:10.3382/ps.2012-02542. PubMed: 23571340.
38. CDC (2013) ultistate Outbreak of Shiga toxin-producing Escherichia coli O121 Infections Linked to Farm Rich Brand Frozen Food Products.
39. Smith MJ, Melton-Celsa AR, Sinclair JF, Carvalho HM, Robinson CM, et al. (2009) Monoclonal antibody 11E10, which neutralizes shiga toxin type 2 (Stx2), recognizes three regions on the Stx2 A subunit, blocks the enzymatic action of the toxin in vitro, and alters the overall cellular distribution of the toxin. Infect Immun 77: 2730-2740. doi:10.1128/IAI.00005-09. PubMed: 19433543.
40. Chen C, Wang S, Wang H, Mao X, Zhang T, et al. (2012) Potent neutralization of botulinum neurotoxin/B by synergistic action of antibodies recognizing protein and ganglioside receptor binding domain. PLOS ONE 7: e43845. doi:10.1371/journal.pone.0043845. PubMed: 22952786.
41. Hull AE, Acheson DW, Echeverria P, Donohue-Rolfe A, Keusch GT, (1993) Mitomycin immunoblot colony assay for detection of Shiga-like toxin-producing Escherichia coli in fecal samples: comparison with DNA probes. J Clin Microbiol 31: 1167-1172. PubMed: 8501216.
42. Detection, Isolation and Identification of Escherichia coli O157:H7 from Meat Products (2012). USDA FSIS Microbiol Laboratory Guidebook Chapter 5: 06.
43. He X, Quinones B, McMahon S, Mandrell RE, (2012) A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli. Toxins (Basel) 4: 487-504. doi:10.3390/toxins4070487.
44. Paton AW, Morona R, Paton JC, (2000) A new biological agent for treatment of Shiga toxigenic Escherichia coli infections and dysentery in humans. Nat Med 6: 265-270. doi:10.1038/73111. PubMed: 10700227.
45. Paton AW, Morona R, Paton JC, (2001) Neutralization of Shiga toxins Stx1, Stx2c, and Stx2e by recombinant bacteria expressing mimics of globotriose and globotetraose. Infect Immun 69: 1967-1970. doi:10.1128/IAI.69.3.1967-1970.2001. PubMed: 11179385.