Exploring valid reference genes for quantitative real-time PCR analysis in Plutella xylostella (Lepidoptera: Plutellidae)

International Journal of Biological Sciences

Volumn 9, Issue 8, 2013, Pages 792-802

  Fu, Wei ^a,b,c   Xie, Wen ^b   Zhang, Zhuo ^c,d   Wang, Shaoli ^b   Wu, Qingjun ^b   Liu, Yong ^c,d   Zhou, Xiaomao ^a   Zhou, Xuguo ^e   Zhang, Youjun ^b  

^a HUNAN AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

^c Hunan Academy of Agricultural Sciences   (China)

^d Key Laboratory of Integrated Management of the Pests   (China)

^e UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Abiotic factor; Biotic factor; Plutella xylostella; qRT PCR analysis; Reference gene

Indexed keywords

ELONGATION FACTOR 1;

ABIOTIC FACTOR.; ANIMAL; ARTICLE; BIOTIC FACTOR; GENE; GENE EXPRESSION PROFILING; GENETICS; METHODOLOGY; MOTH; PLUTELLA XYLOSTELLA; QRT-PCR ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REFERENCE GENE; REFERENCE VALUE; STANDARD;

ABIOTIC FACTOR.; BIOTIC FACTOR; PLUTELLA XYLOSTELLA; QRT-PCR ANALYSIS; REFERENCE GENE;

ANIMALS; GENE EXPRESSION PROFILING; GENES; MOTHS; PEPTIDE ELONGATION FACTOR 1; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE VALUES;

EID: 84884252546     PISSN: 14492288     EISSN: None     Source Type: Journal    
DOI: 10.7150/ijbs.5862     Document Type: Article

References (37)

1. Bustin S. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol. 2000; 5: 169-193.
2. Bustin S, Benes V, Nolan T, et al. Quantitative real-time RT-PCR--a perspective. J Mol Endocrinol. 2005; 34: 597-601.
3. VanGuilder H, Vrana K, Freeman W. Twenty-five years of quantitative PCR for gene expression analysis. Biotechniques. 2008; 44: 619-626.
4. Citri A, Pang Z, Südhof T, et al. Comprehensive qRT-PCR profiling of gene expression in single neuronal cells. Nat Protoc. 2012; 7: 118-117.
5. Bustin S, Benes V, Garson J, et al. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55: 611-622.
6. Bustin S, Beaulieu J, Huggett J, et al. MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments. BMC Mol Biol. 2010; 11: 74.
7. Derveaux S, Vandesompele J, Hellemans J. How to do successful gene expression analysis using real-time PCR. Methods. 2010; 50: 227-230.
8. Radonić A, Thulke S, Mackay I, et al. Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun. 2004; 313: 856-862.
9. Vandesompele J, De Preter K, Pattyn F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3: RESEARCH0034
10. Andersen C, Ledet-Jensen J, Ørntoft T. Normalization of real-time quantitative RT-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004; 64: 5245-5250.
11. Pfaffl M, Tichopad A, Prgomet C, et al. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations. Biotechnol Lett. 2004; 26: 509-515.
12. Suzuki T, Higgins P, Crawford D. Control selection for RNA quantitation. Biotechniques. 2000; 29: 332-337.
13. Lee P, Sladek R, Greenwood C, Hudson T, et al. Control genes and variability: absence of ubiquitous reference transcripts in diverse mammalian expression studies. Genome Res. 2002; 12: 292-297.
14. Ponton F, Chapuis M, Pernice M, et al. Evaluation of potential reference genes for reverse transcription-qRT-PCR studies of physiological responses in Drosophila melanogaster. J Insect Physiol. 2011; 57: 840-850.
15. Dheda K, Huggett J, Chang J, Kim L, et al. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal Biochem. 2005; 344: 141-143.
16. Talekar N, Shelton A. Biology, ecology, and management of the diamondback moth. Annu Rev Entomol. 1993; 38: 275-301.
17. Wu G, Jiang S. Field monitor of insecticide resistance and toxicological mechanism in Plutella xylostella (L.). Acta Phytophylacica Sinica. 2002; 29: 351-355.
18. You M, Yue Z, He W, et al. A heterozygous moth genome provides insights into herbivory and detoxification. Nat Genetics. 2013; 45: 220-225.
19. Sayyed A, Saeed S, Noor-ul-ane M, et al. Genetic, biochemical, and physiological characterization of spinosad resistance in Plutella xylostella(Lepidoptera: Plutellidae). J Econ Entomol. 2008; 101: 1658-1666.
20. Bautista M, Miyata T, Miura K, et al. RNA interference-mediated knockdown of a cytochrome P450, CYP6BG1, from the diamondback moth, Plutella xylostella, reduces larval resistance to permethrin. Insect Mol Biol. 2009; 39: 38-46.
21. Yang Z, Wen L, Wu Q, et al. Effects of injecting cadherin gene dsRNA on growth and development in diamondback moth Plutella xylostella (Lep.: Plutellidae). J Appl Entomol. 2008; 133: 75-81.
22. Sonoda S. Molecular analysis of pyrethroid resistance conferred by target insensitivity and increased metabolic detoxification in Plutella xylostella. Pest Manag Sci. 2010; 66: 572-575.
23. Son Y, Kim Y. Immunosuppression induced by entomopathogens is rescued by addition of apolipophorin III in the diamondback moth, Plutella xylostella. J Invertebr Pathol. 2011; 106: 217-222.
24. Teng X, Zhang Z, He G, et al. Validation of reference genes for quantitative expression analysis by real-time RT-PCR in four Lepidopteran Insects. J Insect Sci. 2012; 12: 1-17.
25. Zhao J, Cao J, Li Y, et al. Transgenic plants expressing two Bacillus thuringiensis toxins delay insect resistance evolution. Nat Biotechnol. 2003; 21: 1493-1497.
26. Yang Z, Wu Q, Wang S, et al. Expression of cadherin, aminopeptidase N and alkaline phosphatase genes in Cry1Ac-susceptible and Cry1Ac-resistant strains of Plutella xylostella (L.). J Appl Entomol. 2012; 136: 539-548.
27. Wang L, Li X, Zhang J, et al. Monitoring of resistance for the diamondback moth to Bacillus thuringiensis Cry1ac and Cry1ba toxins and a Bt commercial formulation. J Appl Entomol. 2007; 131: 441-446.
28. Pfaffl M. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29: e45.
29. Silver N, Best S, Jiang J, et al. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol. 2006; 7: 33.
30. Xie F, Sun G, Stiller J, et al. Genome-wide functional analysis of the cotton transcriptome by creating an integrated EST database. PLoS ONE 2011; 6: e26980.
31. Shen, G, Jiang, H, Wang, X, et al. Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae). BMC Mol Biol 2010; 11: 76.
32. Scharlaken B, Graaf D, Goossens K, et al. Reference gene selection for insect expression studies using quantitative real-time PCR: the head of the honeybee, Apis mellifera, after a bacterial challenge. J Insect Sci. 2008; 8: 33.
33. Van Hiel M, Van Wielendaele P, Temmerman L, et al. Identification and validation of housekeeping genes in brains of the desert locust Schistocerca gregaria under different developmental conditions. BMC Mol Biol. 2009; 10: 56.
34. Chapuis M, Tohidi-Estahani D, Dodgson T, et al. Assessment and validation of a suite of reverse transcription-quantitative PCR reference genes for analyses of density-dependent behavioural plasticity in the Australian plague locust. BMC Genomics. 2011; 12: 7.
35. Jiang H, Liu Y, Tang P, et al. Validation of endogenous reference genes for insecticide-induced and developmental expression profiling of Liposcelis bostsrychophila (Psocoptera: Liposcelididae). Mol Biol Rep. 2010; 37: 1019-1029
36. Majerowicz D, Alves-Bezerra M, Logullo R, et al. Looking for reference genes for real-time quantitative PCR experiments in Rhodnius prolixus (Hemiptera: Reduviidae). Insect Mol Bio. 2011; 20: 713-722.
37. Hornáková D, Matousková P, Kindl J, et al. Selection of reference genes for real-time polymerase chain reaction analysis in tissues from Bombus terrestris and Bombus lucorum of different ages. Anal Biochem. 2010; 397: 118-120.