The RNA methyltransferase dnmt2 is required for efficient dicer-2-dependent siRNA pathway activity in Drosophila

Cell Reports

Volumn 4, Issue 5, 2013, Pages 931-937

  Durdevic, Zeljko ^a   Mobin, MehrpouyaBalaghy ^a,b   Hanna, Katharina ^a   Lyko, Frank ^a   Schaefer, Matthias ^a  

^a GERMAN CANCER RESEARCH CENTER   (Germany)

^b ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ARGONAUTE 2 PROTEIN; COMPLEMENTARY DNA; DICER; DICER 2; DNA METHYLTRANSFERASE 2; DOUBLE STRANDED RNA; RIBOSOME RNA; RNA METHYLTRANSFERASE; SMALL INTERFERING RNA; TRANSFER RNA; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA FRAGMENTATION; DROSOPHILA; GENE SILENCING; GENOTYPE; HEAT SHOCK RESPONSE; IMMUNOPRECIPITATION; MALE; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; RNA SEQUENCE; UPREGULATION;

ANIMALIA;

ANIMALS; DNA (CYTOSINE-5-)-METHYLTRANSFERASE; DROSOPHILA; DROSOPHILA PROTEINS; FEMALE; GENE EXPRESSION; RNA, DOUBLE-STRANDED; RNA, SMALL INTERFERING; RNA, TRANSFER;

EID: 84884167428     PISSN: None     EISSN: 22111247     Source Type: Journal    
DOI: 10.1016/j.celrep.2013.07.046     Document Type: Article

References (27)

1. Babiarz J.E., Ruby J.G., Wang Y., Bartel D.P., Blelloch R. Mouse ES cells express endogenous shRNAs, siRNAs, and other Microprocessor-independent, Dicer-dependent small RNAs. Genes Dev. 2008, 22:2773-2785.
2. Carthew R.W., Sontheimer E.J. Origins and Mechanisms of miRNAs and siRNAs. Cell 2009, 136:642-655.
3. Cenik E.S., Fukunaga R., Lu G., Dutcher R., Wang Y., Tanaka Hall T.M., Zamore P.D. Phosphate and R2D2 restrict the substrate specificity of Dicer-2, an ATP-driven ribonuclease. Mol. Cell 2011, 42:172-184.
4. Cole C., Sobala A., Lu C., Thatcher S.R., Bowman A., Brown J.W.S., Green P.J., Barton G.J., Hutvagner G. Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs. RNA 2009, 15:2147-2160.
5. Czech B., Malone C.D., Zhou R., Stark A., Schlingeheyde C., Dus M., Perrimon N., Kellis M., Wohlschlegel J.A., Sachidanandam R., et al. An endogenous small interfering RNA pathway in Drosophila. Nature 2008, 453:798-802.
6. Durdevic Z., Schaefer M. tRNA modifications: necessary for correct tRNA-derived fragments during the recovery from stress?. Bioessays 2013, 35:323-327.
7. Ghildiyal M., Seitz H., Horwich M.D., Li C., Du T., Lee S., Xu J., Kittler E.L.W., Zapp M.L., Weng Z., Zamore P.D. Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells. Science 2008, 320:1077-1081.
8. Goll M.G., Kirpekar F., Maggert K.A., Yoder J.A., Hsieh C.-L., Zhang X., Golic K.G., Jacobsen S.E., Bestor T.H. Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2. Science 2006, 311:395-398.
9. Haussecker D., Huang Y., Lau A., Parameswaran P., Fire A.Z., Kay M.A. Human tRNA-derived small RNAs in the global regulation of RNA silencing. RNA 2010, 16:673-695.
10. Hurto R.L. Unexpected functions of tRNA and tRNA processing enzymes. Adv. Exp. Med. Biol. 2011, 722:137-155.
11. Ivanov P., Emara M.M., Villen J., Gygi S.P., Anderson P. Angiogenin-induced tRNA fragments inhibit translation initiation. Mol. Cell 2011, 43:613-623.
12. Lee Y.S., Nakahara K., Pham J.W., Kim K., He Z., Sontheimer E.J., Carthew R.W. Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell 2004, 117:69-81.
13. Li Z., Ender C., Meister G., Moore P.S., Chang Y., John B. Extensive terminal and asymmetric processing of small RNAs from rRNAs, snoRNAs, snRNAs, and tRNAs. Nucleic Acids Res. 2012, 40:6787-6799.
14. Lim D.-H., Oh C.-T., Lee L., Hong J.-S., Noh S.-H., Hwang S., Kim S., Han S.-J., Lee Y.S. The endogenous siRNA pathway in Drosophila impacts stress resistance and lifespan by regulating metabolic homeostasis. FEBS Lett. 2011, 585:3079-3085.
15. Lim D.-H., Lee L., Oh C.-T., Kim N.-H., Hwang S., Han S.-J., Lee Y.S. Microarray analysis of Drosophila dicer-2 mutants reveals potential regulation of mitochondrial metabolism by endogenous siRNAs. J.Cell. Biochem. 2013, 114:418-427.
16. Okamura K., Ishizuka A., Siomi H., Siomi M.C. Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. Genes Dev. 2004, 18:1655-1666.
17. Okamura K., Balla S., Martin R., Liu N., Lai E.C. Two distinct mechanisms generate endogenous siRNAs from bidirectional transcription in Drosophila melanogaster. Nat. Struct. Mol. Biol. 2008, 15:581-590.
18. Okamura K., Chung W.-J., Ruby J.G., Guo H., Bartel D.P., Lai E.C. The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs. Nature 2008, 453:803-806.
19. Pederson T. Regulatory RNAs derived from transfer RNA?. RNA 2010, 16:1865-1869.
20. Rehwinkel J., Natalin P., Stark A., Brennecke J., Cohen S.M., Izaurralde E. Genome-wide analysis of mRNAs regulated by Drosha and Argonaute proteins in Drosophila melanogaster. Mol. Cell. Biol. 2006, 26:2965-2975.
21. Saito K., Siomi M.C. Small RNA-mediated quiescence of transposable elements in animals. Dev. Cell 2010, 19:687-697.
22. Schaefer M., Pollex T., Hanna K., Tuorto F., Meusburger M., Helm M., Lyko F. RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage. Genes Dev. 2010, 24:1590-1595.
23. Schönborn J., Oberstrass J., Breyel E., Tittgen J., Schumacher J., Lukacs N. Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res. 1991, 19:2993-3000.
24. Sobala A., Hutvagner G. Small RNAs derived from the 5' end of tRNA can inhibit protein translation in human cells. RNA Biol. 2013, 10:553-563.
25. Thompson D.M., Parker R. Stressing out over tRNA cleavage. Cell 2009, 138:215-219.
26. Tuorto F., Liebers R., Musch T., Schaefer M., Hofmann S., Kellner S., Frye M., Helm M., Stoecklin G., Lyko F. RNA cytosine methylation by Dnmt2 and NSun2 promotes tRNA stability and protein synthesis. Nat. Struct. Mol. Biol. 2012, 19:900-905.
27. Yamasaki S., Ivanov P., Hu G.-F., Anderson P. Angiogenin cleaves tRNA and promotes stress-induced translational repression. J.Cell Biol. 2009, 185:35-42.