A duplex SYBR Green I-based real-time RT-PCR assay for the simultaneous detection and differentiation of Massachusetts and non-Massachusetts serotypes of infectious bronchitis virus

Molecular and Cellular Probes

Volumn 27, Issue 5-6, 2013, Pages 184-192

  Acevedo, Ana M ^a   Perera, Carmen L ^a   Vega, Armando ^a   Ríos, Liliam ^a   Coronado, Liani ^a   Relova, Damarys ^a   Frías, Maria T ^a   Ganges, Llilianne ^b   Núñez, José I ^b   Pérez, Lester J ^a  

^a CENTRO NACIONAL DE SANIDAD AGROPECUARIA CENSA   (Cuba)

^b CENTRE DE RECERCA EN SANITAT ANIMAL CRESA   (Spain)

Author keywords

Differentiation serotypes; Duplex real time RT PCR; Infectious bronchitis virus; SYBR Green I

Indexed keywords

ARTICLE; AVIAN INFECTIOUS BRONCHITIS VIRUS; CONTROLLED STUDY; DUPLEX SYBR GREEN I BASED REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; EPIDEMIC; GENE DOSAGE; GENOTYPE; IN VITRO STUDY; INFECTION CONTROL; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MELTING POINT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION; SENSITIVITY AND SPECIFICITY; SEROTYPE; STRAIN DIFFERENCE; VACCINATION; VIRUS DETECTION; VIRUS IDENTIFICATION; VIRUS STRAIN; VIRUS TYPING;

AVIAN INFECTIOUS BRONCHITIS VIRUS; GALLUS GALLUS;

DIFFERENTIATION SEROTYPES; DUPLEX REAL TIME RT-PCR; INFECTIOUS BRONCHITIS VIRUS; SYBR GREEN-I;

ANIMALS; CHICKENS; CORONAVIRUS INFECTIONS; DISEASE OUTBREAKS; INFECTIOUS BRONCHITIS VIRUS; MASSACHUSETTS; ORGANIC CHEMICALS; POULTRY DISEASES; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SEROTYPING; VACCINES, ATTENUATED; VACCINES, INACTIVATED; VIRAL VACCINES;

EID: 84884150907     PISSN: 08908508     EISSN: 10961194     Source Type: Journal    
DOI: 10.1016/j.mcp.2013.06.001     Document Type: Article

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