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Volumn 179, Issue 1, 2012, Pages 233-241

A multiple SYBR Green I-based real-time PCR system for the simultaneous detection of porcine circovirus type 2, porcine parvovirus, pseudorabies virus and Torque teno sus virus 1 and 2 in pigs

Author keywords

Multiple real time PCR system; Pig; Simultaneous detection; SYBR Green I; Virus

Indexed keywords

ACCURACY; AMPLICON; ANIMAL TISSUE; ARTICLE; CIRCOVIRUS; CIRCOVIRUS TYPE 2; CONTROLLED STUDY; DNA TEMPLATE; LIMIT OF DETECTION; MELTING POINT; NONHUMAN; NUCLEOTIDE SEQUENCE; PORCINE PARVOVIRUS; PRIORITY JOURNAL; PSEUDORABIES HERPETOVIRUS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STRAIN DIFFERENCE; TORQUE TENO VIRUS; TORQUE TENO VIRUS 1; TORQUE TENO VIRUS 2; VALIDITY; VIRUS DETECTION; VIRUS STRAIN;

EID: 84855250427     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.11.009     Document Type: Article
Times cited : (32)

References (50)
  • 2
    • 34447291386 scopus 로고    scopus 로고
    • Molecular diagnosis of viral diseases, present trends and future aspects: a view from the OIE Collaborating Centre for the Application of Polymerase Chain Reaction Methods for Diagnosis of Viral Diseases in Veterinary Medicine
    • Belák S. Molecular diagnosis of viral diseases, present trends and future aspects: a view from the OIE Collaborating Centre for the Application of Polymerase Chain Reaction Methods for Diagnosis of Viral Diseases in Veterinary Medicine. Vaccine 2007, 25:5444-5452.
    • (2007) Vaccine , vol.25 , pp. 5444-5452
    • Belák, S.1
  • 3
    • 0027448557 scopus 로고
    • Genomic organization and mapping of transcription and translation products of the NADL-2 strain of porcine parvovirus
    • Bergeron J., Menezes J., Tijssen P. Genomic organization and mapping of transcription and translation products of the NADL-2 strain of porcine parvovirus. Virology 1993, 197:86-98.
    • (1993) Virology , vol.197 , pp. 86-98
    • Bergeron, J.1    Menezes, J.2    Tijssen, P.3
  • 4
    • 77955278889 scopus 로고    scopus 로고
    • Studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs
    • Blomström A-L., Belák S., Fossum C., Fuxler L., Wallgren P., Berg M. Studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs. Virus Res. 2010, 152:59-64.
    • (2010) Virus Res. , vol.152 , pp. 59-64
    • Blomström, A.-L.1    Belák, S.2    Fossum, C.3    Fuxler, L.4    Wallgren, P.5    Berg, M.6
  • 5
    • 8444235950 scopus 로고    scopus 로고
    • Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR
    • Cao S., Chen H., Zhao J., Lu J., Xiao S., Jin M., Guo A., Wu B., He Q. Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR. Vet. Res. Commun. 2005, 29:263-269.
    • (2005) Vet. Res. Commun. , vol.29 , pp. 263-269
    • Cao, S.1    Chen, H.2    Zhao, J.3    Lu, J.4    Xiao, S.5    Jin, M.6    Guo, A.7    Wu, B.8    He, Q.9
  • 6
    • 79951579078 scopus 로고    scopus 로고
    • Genetic variability and phylogeny of Torque teno sus virus 1 (TTSuV1) and 2 (TTSuV2) based on complete genomes
    • Cortey M., Macera L., Segalés J., Kekarainen T. Genetic variability and phylogeny of Torque teno sus virus 1 (TTSuV1) and 2 (TTSuV2) based on complete genomes. Vet. Microbiol. 2011, 148:125-131.
    • (2011) Vet. Microbiol. , vol.148 , pp. 125-131
    • Cortey, M.1    Macera, L.2    Segalés, J.3    Kekarainen, T.4
  • 7
    • 0017115161 scopus 로고
    • The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine
    • Derbyshire J.B., Caplan B.A. The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine. Can. J. Comp. Med. 1976, 40:252-256.
    • (1976) Can. J. Comp. Med. , vol.40 , pp. 252-256
    • Derbyshire, J.B.1    Caplan, B.A.2
  • 9
    • 70349537317 scopus 로고    scopus 로고
    • Diagnosis of Aujeszky's disease virus infection in samples of doubtful etiology
    • Díaz J.C., Castell S., Barrera M. Diagnosis of Aujeszky's disease virus infection in samples of doubtful etiology. Rev. Salud. Anim. 2006, 28:36-39.
    • (2006) Rev. Salud. Anim. , vol.28 , pp. 36-39
    • Díaz, J.C.1    Castell, S.2    Barrera, M.3
  • 10
    • 57849154032 scopus 로고    scopus 로고
    • Effect of coinfection with genogroup 1 porcine torque teno virus on porcine circovirus type 2-associated postweaning multisystemic wasting syndrome in gnotobiotic pigs
    • Ellis J.A., Allan G., Krakowka S. Effect of coinfection with genogroup 1 porcine torque teno virus on porcine circovirus type 2-associated postweaning multisystemic wasting syndrome in gnotobiotic pigs. Am. J. Vet. Res. 2008, 69:1608-1614.
    • (2008) Am. J. Vet. Res. , vol.69 , pp. 1608-1614
    • Ellis, J.A.1    Allan, G.2    Krakowka, S.3
  • 12
    • 72849121997 scopus 로고    scopus 로고
    • Insights into the evolutionary history of an emerging livestock pathogen: porcine circovirus 2
    • Firth C., Charleston M.A., Duffy S., Shapiro B., Holmes E.C. Insights into the evolutionary history of an emerging livestock pathogen: porcine circovirus 2. J. Virol. 2009, 83:12813-12821.
    • (2009) J. Virol. , vol.83 , pp. 12813-12821
    • Firth, C.1    Charleston, M.A.2    Duffy, S.3    Shapiro, B.4    Holmes, E.C.5
  • 13
    • 77953120842 scopus 로고    scopus 로고
    • Porcine Torque teno virus: determination of viral genomic loads by genogroup-specific multiplex rt-PCR, detection of frequent multiple infections with genogroups 1 or 2, and establishment of viral full-length sequences
    • Gallei A., Pesch S., Esking W.S., Keller C., Ohlinger V.F. Porcine Torque teno virus: determination of viral genomic loads by genogroup-specific multiplex rt-PCR, detection of frequent multiple infections with genogroups 1 or 2, and establishment of viral full-length sequences. Vet. Microbiol. 2010, 143:202-212.
    • (2010) Vet. Microbiol. , vol.143 , pp. 202-212
    • Gallei, A.1    Pesch, S.2    Esking, W.S.3    Keller, C.4    Ohlinger, V.F.5
  • 14
    • 38649122425 scopus 로고    scopus 로고
    • Development of a novel hot-start multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus
    • Giammarioli M., Pellegrini C., Casciari C., De Mia G.M. Development of a novel hot-start multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus. Vet. Res. Commun. 2008, 32:255-262.
    • (2008) Vet. Res. Commun. , vol.32 , pp. 255-262
    • Giammarioli, M.1    Pellegrini, C.2    Casciari, C.3    De Mia, G.M.4
  • 15
    • 0034940799 scopus 로고    scopus 로고
    • Comparative utility of restriction fragment length polymorphism analysis and gene sequencing to molecular epidemiological investigation of a viral outbreak
    • Goldberg T.L., Weigel R.M., Hahn E.C., Scherba G. Comparative utility of restriction fragment length polymorphism analysis and gene sequencing to molecular epidemiological investigation of a viral outbreak. Epidemiol. Infect. 2001, 126:415-424.
    • (2001) Epidemiol. Infect. , vol.126 , pp. 415-424
    • Goldberg, T.L.1    Weigel, R.M.2    Hahn, E.C.3    Scherba, G.4
  • 16
    • 78650568224 scopus 로고    scopus 로고
    • Recent advances in the epidemiology, diagnosis and control of diseases caused by porcine circovirus type 2
    • Grau-Roma L., Fraile L., Segalés J. Recent advances in the epidemiology, diagnosis and control of diseases caused by porcine circovirus type 2. Vet. J. 2011, 187:23-32.
    • (2011) Vet. J. , vol.187 , pp. 23-32
    • Grau-Roma, L.1    Fraile, L.2    Segalés, J.3
  • 17
    • 70349384628 scopus 로고    scopus 로고
    • A review of RT-PCR technologies used in veterinary virology and disease control: sensitive and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health
    • Hoffmann B., Beer M., Reid S.M., Mertens P., Oura C.A., van Rijn P.A., Slomka M.J., Banks J., Brown I.H., Alexander D.J., King D.P. A review of RT-PCR technologies used in veterinary virology and disease control: sensitive and specific diagnosis of five livestock diseases notifiable to the World Organisation for Animal Health. Vet. Microbiol. 2009, 139:1-23.
    • (2009) Vet. Microbiol. , vol.139 , pp. 1-23
    • Hoffmann, B.1    Beer, M.2    Reid, S.M.3    Mertens, P.4    Oura, C.A.5    van Rijn, P.A.6    Slomka, M.J.7    Banks, J.8    Brown, I.H.9    Alexander, D.J.10    King, D.P.11
  • 18
    • 74649085525 scopus 로고    scopus 로고
    • Simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs by multiplex polymerase chain reaction
    • Jiang Y., Shang H., Xu H., Zhu L., Chen W., Zhao L., Fang L. Simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs by multiplex polymerase chain reaction. Vet. J. 2010, 183:172-175.
    • (2010) Vet. J. , vol.183 , pp. 172-175
    • Jiang, Y.1    Shang, H.2    Xu, H.3    Zhu, L.4    Chen, W.5    Zhao, L.6    Fang, L.7
  • 19
    • 33645534842 scopus 로고    scopus 로고
    • Prevalence of swine Torque teno virus in post-weaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs in Spain
    • Kekarainen T., Sibila M., Segalés J. Prevalence of swine Torque teno virus in post-weaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs in Spain. J. Gen. Virol. 2006, 87:833-837.
    • (2006) J. Gen. Virol. , vol.87 , pp. 833-837
    • Kekarainen, T.1    Sibila, M.2    Segalés, J.3
  • 20
    • 0037245205 scopus 로고    scopus 로고
    • Pathogenesis of postweaning multisystemic wasting syndrome reproduced by co-infection with Korean isolates of porcine circovirus 2 and porcine parvovirus
    • Kim J., Choi C., Chae C. Pathogenesis of postweaning multisystemic wasting syndrome reproduced by co-infection with Korean isolates of porcine circovirus 2 and porcine parvovirus. J. Comp. Pathol. 2003, 128:52-59.
    • (2003) J. Comp. Pathol. , vol.128 , pp. 52-59
    • Kim, J.1    Choi, C.2    Chae, C.3
  • 21
    • 0141500083 scopus 로고    scopus 로고
    • Simultaneous detection and differentiation between porcine circovirus and porcine parvovirus in boar semen by multiplex seminested polymerase chain reaction
    • Kim J., Han D.U., Choi C., Chae C. Simultaneous detection and differentiation between porcine circovirus and porcine parvovirus in boar semen by multiplex seminested polymerase chain reaction. J. Vet. Med. Sci. 2003, 65:741-744.
    • (2003) J. Vet. Med. Sci. , vol.65 , pp. 741-744
    • Kim, J.1    Han, D.U.2    Choi, C.3    Chae, C.4
  • 22
    • 57849165064 scopus 로고    scopus 로고
    • Evaluation of induction of porcine dermatitis and nephropathy syndrome in gnotobiotic pigs with negative results for porcine circovirus type 2
    • Krakowka S., Hartunian C., Hamberg A., Shoup D., Rings M., Zhang Y., Allan G., Ellis J.A. Evaluation of induction of porcine dermatitis and nephropathy syndrome in gnotobiotic pigs with negative results for porcine circovirus type 2. Am. J. Vet. Res. 2008, 69:1615-1622.
    • (2008) Am. J. Vet. Res. , vol.69 , pp. 1615-1622
    • Krakowka, S.1    Hartunian, C.2    Hamberg, A.3    Shoup, D.4    Rings, M.5    Zhang, Y.6    Allan, G.7    Ellis, J.A.8
  • 23
    • 33845301704 scopus 로고    scopus 로고
    • Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs
    • Lee C.-S., Moon H.-J., Yang J.-S., Park S.-J., Song D.-S., Kang B.-K., Park B.-K. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs. J. Virol. Methods 2007, 139:39-43.
    • (2007) J. Virol. Methods , vol.139 , pp. 39-43
    • Lee, C.-S.1    Moon, H.-J.2    Yang, J.-S.3    Park, S.-J.4    Song, D.-S.5    Kang, B.-K.6    Park, B.-K.7
  • 24
    • 43649106364 scopus 로고    scopus 로고
    • Simultaneous detection and genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR and amplicon melting curve analysis using SYBR Green
    • Martinez E., Riera P., Sitja M., Fang Y., Oliveira S., Maldonado J. Simultaneous detection and genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR and amplicon melting curve analysis using SYBR Green. Res. Vet. Sci. 2008, 85:184-193.
    • (2008) Res. Vet. Sci. , vol.85 , pp. 184-193
    • Martinez, E.1    Riera, P.2    Sitja, M.3    Fang, Y.4    Oliveira, S.5    Maldonado, J.6
  • 25
    • 55549095620 scopus 로고    scopus 로고
    • Development and validation of a SYBR Green real-time PCR for the quantification of porcine circovirus type 2 in serum, buffy coat, feces, and multiple tissues
    • McIntosh K.A., Tumber A., Harding J.C.S., Krakowka S., Ellis J.A., Hill J.E. Development and validation of a SYBR Green real-time PCR for the quantification of porcine circovirus type 2 in serum, buffy coat, feces, and multiple tissues. Vet. Microbiol. 2009, 133:23-33.
    • (2009) Vet. Microbiol. , vol.133 , pp. 23-33
    • McIntosh, K.A.1    Tumber, A.2    Harding, J.C.S.3    Krakowka, S.4    Ellis, J.A.5    Hill, J.E.6
  • 26
    • 40749147321 scopus 로고    scopus 로고
    • Porcine parvovirus
    • Blackwell Publishing, Ames, IA, B.E. Straw, J.J. Zimmerman, S. D'Allaire, D.J. Taylor (Eds.)
    • Mengeling W.L. Porcine parvovirus. Diseases of Swine 2006, Blackwell Publishing, Ames, IA. 9th edition. B.E. Straw, J.J. Zimmerman, S. D'Allaire, D.J. Taylor (Eds.).
    • (2006) Diseases of Swine
    • Mengeling, W.L.1
  • 27
    • 0033631564 scopus 로고    scopus 로고
    • Aujeszky's disease (Pseudorabies) virus: the virus and molecular pathogenesis-state of the art
    • Mettenleiter T.C. Aujeszky's disease (Pseudorabies) virus: the virus and molecular pathogenesis-state of the art. Vet. Res. 2000, 31:99-115.
    • (2000) Vet. Res. , vol.31 , pp. 99-115
    • Mettenleiter, T.C.1
  • 28
    • 67349209625 scopus 로고    scopus 로고
    • Real-time PCR to detect and analyze virulent PPV loads in artificially challenged sows and their fetuses
    • Miao L.-f., Zhang C.-f., Chen C.-m., Cui S.-j. Real-time PCR to detect and analyze virulent PPV loads in artificially challenged sows and their fetuses. Vet. Microbiol. 2009, 138:145-149.
    • (2009) Vet. Microbiol. , vol.138 , pp. 145-149
    • Miao, L.-F.1    Zhang, C.-F.2    Chen, C.-M.3    Cui, S.-J.4
  • 29
    • 34147191455 scopus 로고    scopus 로고
    • Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract
    • Nauwynck H., Glorieux S., Favoreel H., Pensaert M. Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract. Vet. Res. 2007, 38:229-241.
    • (2007) Vet. Res. , vol.38 , pp. 229-241
    • Nauwynck, H.1    Glorieux, S.2    Favoreel, H.3    Pensaert, M.4
  • 30
    • 84855231004 scopus 로고
    • Aislamiento del virus de la rinotraqueitis infecciosa bovina de terneros con queratoconjuntivitis
    • Noda J., Nuñez A., García J. Aislamiento del virus de la rinotraqueitis infecciosa bovina de terneros con queratoconjuntivitis. Rev. Salud. Anim. 1984, 6:651-653.
    • (1984) Rev. Salud. Anim. , vol.6 , pp. 651-653
    • Noda, J.1    Nuñez, A.2    García, J.3
  • 31
    • 58349087658 scopus 로고    scopus 로고
    • History of discoveries and pathogenicity of TT viruses
    • Okamoto H. History of discoveries and pathogenicity of TT viruses. Curr. Top Microbiol. Immunol. 2009, 331:1-20.
    • (2009) Curr. Top Microbiol. Immunol. , vol.331 , pp. 1-20
    • Okamoto, H.1
  • 32
    • 1442286315 scopus 로고    scopus 로고
    • Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs
    • Olvera A., Sibila M., Calsamiglia M., Segales J., Domingo M. Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs. J. Virol. Methods 2004, 117:75-80.
    • (2004) J. Virol. Methods , vol.117 , pp. 75-80
    • Olvera, A.1    Sibila, M.2    Calsamiglia, M.3    Segales, J.4    Domingo, M.5
  • 33
    • 0038666509 scopus 로고    scopus 로고
    • Single nucleotide polymorphism genotyping using allele-specific PCR and fluorescence melting curves
    • Papp A.C., Pinsonneault J.K., Cooke G., Sadee W. Single nucleotide polymorphism genotyping using allele-specific PCR and fluorescence melting curves. Biotechniques 2003, 34:1068-1072.
    • (2003) Biotechniques , vol.34 , pp. 1068-1072
    • Papp, A.C.1    Pinsonneault, J.K.2    Cooke, G.3    Sadee, W.4
  • 35
    • 80555155598 scopus 로고    scopus 로고
    • Molecular detection of Torque teno sus virus in lymphoid tissues in concomitant infections with other porcine viral pathogens
    • Pérez L.J., Díaz de Arce H., Frías M.T., Perera C.L., Ganges L., Núñez J.I. Molecular detection of Torque teno sus virus in lymphoid tissues in concomitant infections with other porcine viral pathogens. Res. Vet. Sci. 2011, 10.1016/j.rvsc.2011.02.012.
    • (2011) Res. Vet. Sci.
    • Pérez, L.J.1    Díaz de Arce, H.2    Frías, M.T.3    Perera, C.L.4    Ganges, L.5    Núñez, J.I.6
  • 36
    • 77955918177 scopus 로고    scopus 로고
    • Genetic characterization and phylogenetic analysis of porcine circovirus type 2 strains present in Cuban swine herds
    • Pérez L.J., Díaz de Arce H., Frías M.T. Genetic characterization and phylogenetic analysis of porcine circovirus type 2 strains present in Cuban swine herds. Res. Vet. Sci. 2010, 89:301-305.
    • (2010) Res. Vet. Sci. , vol.89 , pp. 301-305
    • Pérez, L.J.1    Díaz de Arce, H.2    Frías, M.T.3
  • 38
    • 70349490569 scopus 로고    scopus 로고
    • Development of polymerase chain reaction assay for the detection of pseudorabies virus in clinical samples
    • Pérez L.J., Díaz de Arce H. Development of polymerase chain reaction assay for the detection of pseudorabies virus in clinical samples. Braz. J. Microbiol. 2009, 40:433-438.
    • (2009) Braz. J. Microbiol. , vol.40 , pp. 433-438
    • Pérez, L.J.1    Díaz de Arce, H.2
  • 44
    • 68949166160 scopus 로고    scopus 로고
    • Fluorescence-based multiplex real-time RT-PCR arrays for the detection and serotype determination of foot-and-mouth disease virus
    • Tam S., Clavijo A., Engelhard E.K., Thurmond M.C. Fluorescence-based multiplex real-time RT-PCR arrays for the detection and serotype determination of foot-and-mouth disease virus. J. Virol. Methods 2009, 161:183-191.
    • (2009) J. Virol. Methods , vol.161 , pp. 183-191
    • Tam, S.1    Clavijo, A.2    Engelhard, E.K.3    Thurmond, M.C.4
  • 46
    • 32344444310 scopus 로고    scopus 로고
    • Real-time RT-PCR and SYBR Green I melting curve analysis for the identification of Plum pox virus strains C, EA, and W: Effect of amplicon size, melt rate, and dye translocation
    • Varga A., James D. Real-time RT-PCR and SYBR Green I melting curve analysis for the identification of Plum pox virus strains C, EA, and W: Effect of amplicon size, melt rate, and dye translocation. J. Virol. Methods 2006, 132:146-153.
    • (2006) J. Virol. Methods , vol.132 , pp. 146-153
    • Varga, A.1    James, D.2
  • 47
    • 0036925248 scopus 로고    scopus 로고
    • Kinetic characterization of primer mismatches in allel-specific PCR: a quantitative assessment
    • Waterfall C.M., Eisenthal R., Cobb B.D. Kinetic characterization of primer mismatches in allel-specific PCR: a quantitative assessment. Biochem. Biophys. Res. Commun. 2002, 299:715-722.
    • (2002) Biochem. Biophys. Res. Commun. , vol.299 , pp. 715-722
    • Waterfall, C.M.1    Eisenthal, R.2    Cobb, B.D.3
  • 48
    • 77952089735 scopus 로고    scopus 로고
    • A study on the severity and relevance of porcine circovirus type 2 infections in Dutch fattening pigs with respiratory diseases
    • Wellenberg G.J., Bouwkampa F.T., Wolf P.J., Swart W.A.J.M., Mombarg M.J., de Gee A.L.W. A study on the severity and relevance of porcine circovirus type 2 infections in Dutch fattening pigs with respiratory diseases. Vet. Microbiol. 2010, 142:217-224.
    • (2010) Vet. Microbiol. , vol.142 , pp. 217-224
    • Wellenberg, G.J.1    Bouwkampa, F.T.2    Wolf, P.J.3    Swart, W.A.J.M.4    Mombarg, M.J.5    de Gee, A.L.W.6
  • 49
    • 64949128856 scopus 로고    scopus 로고
    • A multiplex PCR for rapid and simultaneous detection of porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus in clinical specimens
    • Yue F., Cui S., Zhang C., Yoon K.J. A multiplex PCR for rapid and simultaneous detection of porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus in clinical specimens. Virus Genes 2009, 38:392-397.
    • (2009) Virus Genes , vol.38 , pp. 392-397
    • Yue, F.1    Cui, S.2    Zhang, C.3    Yoon, K.J.4
  • 50
    • 33846804274 scopus 로고    scopus 로고
    • Study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts
    • Zeeuw E.J., Leinecker N., Herwig V., Selbitz H.-J., Truyen U. Study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts. J. Gen. Virol. 2007, 88:420-427.
    • (2007) J. Gen. Virol. , vol.88 , pp. 420-427
    • Zeeuw, E.J.1    Leinecker, N.2    Herwig, V.3    Selbitz, H.-J.4    Truyen, U.5


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