Trans-chalcone and quercetin down-regulate fatty acid synthase gene expression and reduce ergosterol content in the human pathogenic dermatophyte Trichophyton rubrum

BMC Complementary and Alternative Medicine

Volumn 13, Issue , 2013, Pages

  Bitencourt, Tamires A ^a   Komoto, Tatiana T ^a   Massaroto, Bruna G ^a   Miranda, Carlos Eduardo S ^a   Beleboni, Rene O ^a   Marins, Mozart ^a   Fachin, Ana L ^a  

^a UNIVERSITY OF RIBEIRÃO PRETO   (Brazil)

Author keywords

Antifungal activity; Antifungal agent; Antifungal target; Dermatophytes; Trichophyton rubrum

Indexed keywords

ABC TRANSPORTER; ANTIFUNGAL AGENT; CERULENIN; CHALCONE; ELLAGIC ACID; ERGOSTEROL; FATTY ACID SYNTHASE; FLUCONAZOLE; GALANGIN; GENISTEIN; LUTEOLIN; QUERCETIN;

ANTIFUNGAL ACTIVITY; ARTICLE; BROTH DILUTION; CELL MEMBRANE; DOWN REGULATION; ENZYMATIC ASSAY; ENZYME ACTIVITY; FATTY ACID SYNTHESIS; FUNGAL COLONIZATION; FUNGAL STRAIN; GENE EXPRESSION; HUMAN; IC 50; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PROTOPLAST; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPECTROPHOTOMETRY; TRICHOPHYTON RUBRUM;

ANTIFUNGAL AGENTS; CHALCONE; ERGOSTEROL; FATTY ACID SYNTHASES; FUNGAL PROTEINS; GENE EXPRESSION; METHYLTRANSFERASES; MICROBIAL SENSITIVITY TESTS; QUERCETIN; TRICHOPHYTON;

EID: 84883853206     PISSN: None     EISSN: 14726882     Source Type: Journal    
DOI: 10.1186/1472-6882-13-229     Document Type: Article

References (30)

1. Marconi VC, Kradin R, Marty FM, Hospenthal DR, Kotton CN. Disseminated dermatophytosis in a patient with hereditary hemochromatosis and hepatic cirrhosis: case report and review of the literature. Med Mycol 2010, 48(3):518-527. 10.3109/13693780903213512, 20092423.
2. Ameen M. Epidemiology of superficial fungal infections. Clinics in dermatology 2010, 28(2):197-201.
3. Chen BK, Friedlander SF. Tinea capitis update: a continuing conflict with an old adversary. Curr Opin Pediatr 2001, 13(4):331-335. 10.1097/00008480-200108000-00008, 11717558.
4. Baddley JW, Moser SA. Emerging fungal resistance. Clin Lab Med 2004, 24(3):721-735. 10.1016/j.cll.2004.05.003, 15325062.
5. Projan SJ, Youngman PJ. Antimicrobials: new solutions badly needed. Curr Opin Microbiol 2002, 5(5):463-465. 10.1016/S1369-5274(02)00364-8, 12354551.
6. Roemer T, Xu D, Singh SB, Parish CA, Harris G, Wang H, Davies JE, Bills GF. Confronting the challenges of natural product-based antifungal discovery. Chem Biol 2011, 18(2):148-164. 10.1016/j.chembiol.2011.01.009, 21338914.
7. Monk BC, Cannon RD. Genomic pathways to antifungal discovery. Curr Drug Targets: Infect Disord 2002, 2(4):309-329.
8. Sturtevant J. Translation elongation-3-like factors: are they rational antifungal targets?. Expert Opin Ther Tar 2002, 6(5):545-553.
9. Leibundgut M, Maier T, Jenni S, Ban N. The multienzyme architecture of eukaryotic fatty acid synthases. Curr Opin Struct Biol 2008, 18(6):714-725. 10.1016/j.sbi.2008.09.008, 18948193.
10. Maier T, Leibundgut M, Boehringer D, Ban N. Structure and function of eukaryotic fatty acid synthases. Q Rev Biophys 2010, 43(3):373-422. 10.1017/S0033583510000156, 20731893.
11. Nomura S, Horiuchi T, Omura S, Hata T. he action mechanism of cerulenin. I. Effect of cerulenin on sterol and fatty acid biosynthesis in yeast. J Biochem 1972, 71(5):783-796.
12. Li XC, Joshi AS, ElSohly HN, Khan SI, Jacob MR, Zhang Z, Khan IA, Ferreira D, Walker LA, Broedel SE, et al. Fatty acid synthase inhibitors from plants: isolation, structure elucidation, and SAR studies. J Nat Prod 2002, 65(12):1909-1914. 10.1021/np020289t, 12502337.
13. Zhang W, Yu L, Leng W, Wang X, Wang L, Deng X, Yang J, Liu T, Peng J, Wang J, et al. cDNA microarray analysis of the expression profiles of Trichophyton rubrum in response to novel synthetic fatty acid synthase inhibitor PHS11A. Fungal genetics and biology: FG & B 2007, 44(12):1252-1261. 10.1016/j.fgb.2007.03.002, 18824240.
14. Yu L, Zhang W, Liu T, Wang X, Peng J, Li S, Jin Q. Global gene expression of Trichophyton rubrum in response to PH11B, a novel fatty acid synthase inhibitor. J Applied Microbiol 2007, 103(6):2346-2352.
15. Fachin AL, Ferreira-Nozawa MS, Maccheroni W, Martinez-Rossi NM. Role of the ABC transporter TruMDR2 in terbinafine, 4-nitroquinoline N-oxide and ethidium bromide susceptibility in Trichophyton rubrum. J Med Microbiol 2006, 55(Pt 8):1093-1099.
16. Arthington-Skaggs BA, Jradi H, Desai T, Morrison CJ. Quantitation of ergosterol content: novel method for determination of fluconazole susceptibility of Candida albicans. J Clin Microbiol 1999, 37(10):3332-3337. 85559, 10488201.
17. Cove DJ. The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et biophysica acta 1966, 113(1):51-56. 10.1016/S0926-6593(66)80120-0, 5940632.
18. Nowakowska Z. A review of anti-infective and anti-inflammatory chalcones. Eur J Med Chem 2007, 42(2):125-137. 10.1016/j.ejmech.2006.09.019, 17112640.
19. Boeck P, Leal PC, Yunes RA, Filho VC, Lopez S, Sortino M, Escalante A, Furlan RL, Zacchino S. Antifungal activity and studies on mode of action of novel xanthoxyline-derived chalcones. Arch Pharm 2005, 338(2-3):87-95.
20. Brown AK, Papaemmanouil A, Bhowruth V, Bhatt A, Dover LG, Besra GS. Flavonoid inhibitors as novel antimycobacterial agents targeting Rv0636, a putative dehydratase enzyme involved in Mycobacterium tuberculosis fatty acid synthase II. Microbiology 2007, 153(Pt 10):3314-3322.
21. Jung HJ, Park Y, Sung WS, Suh BK, Lee J, Hahm K-S, Lee DG. Fungicidal effect of pleurocidin by membrane-active mechanism and design of enantiomeric analogue for proteolytic resistance. Biochimica et Biophysica Acta (BBA) - Biomembranes 2007, 1768(6):1400-1405.
22. Lee DG, Park Y, Kim HN, Kim HK, Kim PI, Choi BH, Hahm K-S. Antifungal Mechanism of an Antimicrobial Peptide, HP (2-20), Derived from N-Terminus of Helicobacter pylori Ribosomal Protein L1 against Candida albicans. Biochem Biophys Res Communications 2002, 291(4):1006-1013.
23. Young LY, Hull CM, Heitman J. Disruption of ergosterol biosynthesis confers resistance to amphotericin B in Candida lusitaniae. Antimicrobial agents and chemotherapy 2003, 47(9):2717-2724. 10.1128/AAC.47.9.2717-2724.2003, 182600, 12936965.
24. Zhang W, Yu L, Yang J, Wang L, Peng J, Jin Q. Transcriptional profiles of response to terbinafine in Trichophyton rubrum. Appl Microbiol Biotechnol 2009, 82(6):1123-1130. 10.1007/s00253-009-1908-9, 19234875.
25. Barker KS, Crisp S, Wiederhold N, Lewis RE, Bareither B, Eckstein J, Barbuch R, Bard M, Rogers PD. Genome-wide expression profiling reveals genes associated with amphotericin B and fluconazole resistance in experimentally induced antifungal resistant isolates of Candida albicans. J Antimicrob Chemother 2004, 54(2):376-385. 10.1093/jac/dkh336, 15201233.
26. Leber R, Zinser E, Hrastnik C, Paltauf F, Daum G. Export of steryl esters from lipid particles and release of free sterols in the yeast, Saccharomyces cerevisiae. Biochimica et Biophysica Acta (BBA) - Biomembranes 1995, 1234(1):119-126.
27. Pinto WJ, Lozano R, Nes WR. Inhibition of sterol biosynthesis by ergosterol and cholesterol in Saccharomyces cerevisiae. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1985, 836(1):89-95.
28. Servouse M, Karst F. Regulation of early enzymes of ergosterol biosynthesis in Saccharomyces cerevisiae. Biochem J 1986, 240(2):541-547. 1147448, 2880580.
29. Casey WM, Keesler GA, Parks LW. Regulation of partitioned sterol biosynthesis in Saccharomyces cerevisiae. J Bacteriol 1992, 174(22):7283-7288. 207422, 1429452.
30. Smith SJ, Crowley JH, Parks LW. Transcriptional regulation by ergosterol in the yeast Saccharomyces cerevisiae. Mol Cell Biol 1996, 16(10):5427-5432. 231542, 8816455.