Polymerase chain reaction test for clostridium difficile toxin B gene reveals similar prevalence rates in children with and without inflammatory bowel disease

Journal of Pediatric Gastroenterology and Nutrition

Volumn 57, Issue 3, 2013, Pages 293-297

  Lamousé Smith, Esi S N ^a   Weber, Sarah ^a   Rossi, Richard F ^b   Neinstedt, Liliane J ^a   Mosammaparast, Nima ^c   Sandora, Thomas J ^b   McAdam, Alexander J ^b   Bousvaros, Athos ^a  

^a Divisions of Gastroenterology and Nutrition   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

^c WASHINGTON UNIVERSITY   (United States)

Author keywords

Clostridium difficile; Crohn disease; diarrhea; inflammatory bowel disease; ulcerative colitis

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN B; BACTERIAL PROTEIN; BACTERIAL TOXIN; ENTEROTOXIN; TOXB PROTEIN, CLOSTRIDIUM DIFFICILE;

ADOLESCENT; ADULT; ARTICLE; BACTERIAL GENE; CHILD; CHILDHOOD DISEASE; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; CROHN DISEASE; DIARRHEA; ENTERITIS; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES ANALYSIS; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PRIORITY JOURNAL; SCHOOL CHILD; SEROPREVALENCE; ULCERATIVE COLITIS; CHI SQUARE DISTRIBUTION; CLOSTRIDIUM INFECTIONS; COMPARATIVE STUDY; COMPLICATION; FECES; GENETICS; INFLAMMATORY BOWEL DISEASE; MICROBIOLOGY; PEPTOCLOSTRIDIUM DIFFICILE; PREVALENCE; PROCEDURES;

CLOSTRIDIUM DIFFICILE;

ADOLESCENT; BACTERIAL PROTEINS; BACTERIAL TOXINS; CHI-SQUARE DISTRIBUTION; CHILD; CHILD, PRESCHOOL; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; DIARRHEA; ENTEROTOXINS; FECES; FEMALE; GENES, BACTERIAL; HUMANS; INFLAMMATORY BOWEL DISEASES; MALE; POLYMERASE CHAIN REACTION; PREVALENCE;

EID: 84883741182     PISSN: 02772116     EISSN: 15364801     Source Type: Journal    
DOI: 10.1097/MPG.0b013e3182999990     Document Type: Article

References (31)

1. Musa S, Thomson S, Cowan M., et al. Clostridium difficile infection and inflammatory bowel disease. Scand J Gastroenterol 2010; 45: 261-72.
2. Navaneethan U, Venkatesh PG, Shen B. Clostridium difficile infection and inflammatory bowel disease: understanding the evolving relationship. World J Gastroenterol 2010; 16: 4892-904.
3. Frank DN, St Amand AL, Feldman RA, et al. Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. Proc Natl Acad Sci U S A 2007; 104: 13780-5. (Pubitemid 350003397)
4. Schwiertz A, Jacobi M, Frick J.S., et al. Microbiota in pediatric inflammatory bowel disease. J Pediatr 2010; 157: 240e1-4e1.
5. Papa E, Docktor M, Smillie C., et al. Non-invasive mapping of the gastrointestinal microbiota identifies children with inflammatory bowel disease. PLoS One 2012; 7:e39242.
6. Sartor RB. Genetics and environmental interactions shape the intestinal microbiome to promote inflammatory bowel disease versus mucosal homeostasis. Gastroenterology 2010; 139: 1816-9.
7. Mezoff E, Mann EA, Hart K.W., et al. Clostridium difficile infection and treatment in the pediatric inflammatory bowel disease population. J Pediatr Gastroenterol Nutr 2011; 52: 437-41.
8. Pascarella F, Martinelli M, Miele E., et al. Impact of Clostridium difficile infection on pediatric inflammatory bowel disease. J Pediatr 2009; 154: 854-8.
9. Markowitz JE, Brown KA, Mamula P, et al Failure of single-toxin assays to detect Clostridium difficile infection in pediatric inflammatory bowel disease. Am J Gastroenterol 2001; 96: 2688-90.
10. Gryboski JD. Clostridium difficile in inflammatory bowel disease relapse. J Pediatr Gastroenterol Nutr 1991; 13: 39-41.
11. Hyams JS, McLaughlin JC Lack of relationship between Clostridium difficile toxin and inflammatory bowel disease in children. J Clin Gastroenterol 1985; 7: 387-90. (Pubitemid 16248642)
12. Bryant K, McDonald LC Clostridium difficile infections in children. Pediatr Infect Dis J 2009; 28: 145-6.
13. Belanger SD, Boissinot M, Clairoux N., et al. Rapid detection of Clostridium difficile infeces byreal-time PCR. J Clin Microbiol 2003; 41: 730-4. (Pubitemid 36184188)
14. Peterson LR, Manson RU, Paule S.M., et al. Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea. Clin Infect Dis 2007; 45: 1152-60. (Pubitemid 47580313)
15. Peterson LR, Mehta MS, Patel P.A., et al. Laboratory testing for Clostridium difficile infection: light at the end of the tunnel. Am J Clin Pathol 2011; 136: 372-80.
16. Luna RA, Boyanton BL Jr, Mehta S, et al. Rapid stool-based diagnosis of Clostridium difficile infection by real-time PCR in a children's hospital. J Clin Microbiol 2011; 49: 851-7.
17. Stamper PD, Alcabasa R, Aird D., et al. Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples. J Clin Microbiol 2009; 47: 373-8.
18. Selvaraju SB, Gripka M, Estes K., et al. Detection of toxigenic Clostridium difficile in pediatric stool samples: an evaluation of Quik Check Complete Antigen assay, BD GeneOhm Cdiff PCR, and Pro-Gastro Cd PCR assays. Diagn Microbiol Infect Dis 2011; 71: 224-9.
19. Barbut F, Braun M, Burghoffer B., et al. Rapid detection of toxigenic strains of Clostridium difficile in diarrheal stools by real-time PCR. J Clin Microbiol 2009; 47: 1276-7.
20. Enoch DA, Butler MJ, Pai S, et al Clostridium difficile in children: colonisation and disease. J Infect 2011; 63: 105-13.
21. Denno DM, Stapp JR, Boster D.R., et al. Etiology of diarrhea in pediatric outpatient settings. Pediatr Infect Dis J 2005; 24: 142-8. (Pubitemid 40250773)
22. McDonald LC, Killgore GE, Thompson A, et al Anepidemic, toxin genevariant strain of Clostridium difficile. N Engl J Med 2005; 353: 2433-41. (Pubitemid 41770164)
23. Goodhand JR, Alazawi W, Rampton DS Systematic review: Clostridium difficile and inflammatory bowel disease. Aliment Pharmacol Ther 2011; 33: 428-41.
24. Clayton EM, Rea MC, Shanahan F, et al The vexed relationship between Clostridium difficile and inflammatory bowel disease: an assessment of carriage in an outpatient setting among patients in remission. Am J Gastroenterol 2009; 104: 1162-9.
25. Banaszkiewicz A, Kowalska-Duplaga K, Pytrus T., et al. Clostridium difficile infectioninnewly diagnosed pediatric patients with inflammatory bowel disease: prevalence and risk factors. Inflamm Bowel Dis 2012; 18: 844-8.
26. Kvach EJ, Ferguson D, Riska P.F., et al. Comparison of BD GeneOhm Cdiff real-time PCR assay with a two-step algorithm and a toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection. J Clin Microbiol 2010; 48: 109-14.
27. Cho SY, Nam YS, Kim M.J., et al. Detection of Clostridium difficile as a routine diagnosis: comparison of real-time PCR and enzyme immunoassay. Am J Clin Pathol 2012; 137: 494-5.
28. Barbut F, Monot M, Rousseau A., et al. Rapid diagnosis of Clostridium difficile infection by multiplex real-time PCR. Eur J Clin Microbiol Infect Dis 2011; 30: 1279-85.
29. Freeman J, Wilcox MH The effects of storage conditions on viability of Clostridium difficile vegetative cells and spores and toxin activity in human faeces. J Clin Pathol 2003; 56: 126-8. (Pubitemid 36177255)
30. Cohen SH, Gerding DN, Johnson S, et al Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the society for healthcare epidemiology of America (SHEA) and the infectious diseases society of America (IDSA). Infect Control Hosp Epidemiol 2010; 31: 431-55.
31. Berg AM, Kelly CP, Farraye FA Clostridium difficile infection in the inflammatory bowel disease patient. Inflamm Bowel Dis 2012; 19: 194-204.