Rapid detection of toxigenic strains of clostridium difficile in diarrheal stools by real-time PCR

Journal of Clinical Microbiology

Volumn 47, Issue 4, 2009, Pages 1276-1277

  Barbut, Frederic ^a   Braun, Mylena ^a   Burghoffer, Beatrice ^a   Lalande, Valerie ^a   Eckert, Catherine ^a  

^a ASSISTANCE PUBLIQUE HÔPITAUX DE PARIS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLICON; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTION; CYTOTOXICITY TEST; DIAGNOSTIC ACCURACY; DIARRHEA; FECES ANALYSIS; FLUOROMETRY; GENE AMPLIFICATION; GOLD STANDARD; HUMAN; LETTER; NONHUMAN; PRIORITY JOURNAL; PROSPECTIVE STUDY; PSEUDOMEMBRANOUS COLITIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; BIOSYNTHESIS; FECES; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION;

CLOSTRIDIUM DIFFICILE;

BACTERIAL TOXIN;

BACTERIAL TOXINS; CLOSTRIDIUM DIFFICILE; ENTEROCOLITIS, PSEUDOMEMBRANOUS; FECES; HUMANS; POLYMERASE CHAIN REACTION;

EID: 65249184354     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00309-09     Document Type: Letter

References (5)

1. Bélanger, S. D., M. Boissinot, N. Clairoux, F. J. Picard, and M. G. Bergeron. 2003. Rapid detection of Clostridium difficile in feces by real-time PCR. J. Clin. Microbiol. 41:730-734.
2. Peterson, L. R., R. U. Manson, S. M. Paule, D. M. Hacek, A. Robicsek, R. B. Thomson, Jr., and K. L. Kaul. 2007. Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea. Clin. Infect. Dis. 45:11521160.
3. Sloan, L. M., B. J. Duresko, D. R. Gustafson, and J. E. Rosenblatt. 2008. Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 46:1996-2001.
4. Stamper, P. D., R. Alcabasa, D. Aird, W. Babiker, J. Wehrlin, I. Ikpeama, and K. C. Carroll. 2009. Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples. J. Clin. Microbiol. 47:373-378.
5. van den Berg, R. J., N. Vaessen, H. P. Endtz, T. Schulin, E. R. van der Vorm, and E. J. Kuijper. 2007. Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study. J. Med. Microbiol. 56:36-42.