Detection of toxigenic Clostridium difficile in pediatric stool samples: An evaluation of Quik Check Complete Antigen assay, BD GeneOhm Cdiff PCR, and ProGastro Cd PCR assays

Diagnostic Microbiology and Infectious Disease

Volumn 71, Issue 3, 2011, Pages 224-229

  Selvaraju, Suresh B ^a,b   Gripka, Megan ^a   Estes, Kristen ^a   Nguyen, Ashley ^a   Jackson, Mary Anne ^a,b   Selvarangan, Rangaraj ^a,b  

^a CHILDREN S MERCY HOSPITAL   (United States)

^b UNIVERSITY OF MISSOURI KANSAS CITY SCHOOL OF MEDICINE   (United States)

Author keywords

BD GeneOhm PCR; C Diff Quik Check Complete; Clostridium difficile; ProGastro PCR

Indexed keywords

ARTICLE; BACTERIUM CULTURE; CHILD; CLINICAL ARTICLE; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; FECES CULTURE; HUMAN; INTERMETHOD COMPARISON; MEDICAL RECORD REVIEW; NONHUMAN; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; TOXIGENIC CULTURE;

ANTIGENS, BACTERIAL; CHILD, PRESCHOOL; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; ENTEROTOXINS; FECES; HUMANS; INFANT; INFANT, NEWBORN; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 80053895967     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2011.07.015     Document Type: Article

References (26)

1. Alcala L., Sanchez-Cambronero L., Catalan M.P., Sanchez-Somolinos M., Pelaez M.T., Martin M., Bouza E. Comparison of three commercial methods for rapid detection of Clostridium difficile toxins A and B from fecal specimens. J. Clin. Microbiol. 2008, 46:3833-3835.
2. Babady N.E., Stiles J., Ruggiero P., Khosa P., Huang D., Shuptar S., Kamboj M., Kiehn T.E. Evaluation of the Cepheid Xpert Clostridium difficile Epi assay for diagnosis of C. difficile infection and typing of the NAP1 strain at a cancer hospital. J. Clin. Microbiol. 2010, 48:4519-4524.
3. Centers for Disease Control and Prevention (CDC) Surveillance for community-associated Clostridium difficile-Connecticut, 2006. MMWR Morb. Mortal. Wkly. Rep. 2008, 57:340-343.
4. Doing K.M., Hintz M.S., Keefe C., Horne S., Levasseur S., Kulikowski M.L. Reevaluation of the Premier Clostridium difficile toxin A and B immunoassay with comparison to glutamate dehydrogenase common antigen testing evaluating Bartels cytotoxin and Prodesse ProGastro Cd polymerase chain reaction as confirmatory procedures. Diagn. Microbiol. Infect. Dis. 2010, 66:129-134.
5. Eastwood K., Else P., Charlett A., Wilcox M. Comparison of nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J. Clin. Microbiol. 2009, 47:3211-3217.
6. Gilligan P.H. Is a two-step glutamate dehydrogenase antigen-cytotoxicity neutralization assay algorithm superior to the premier toxin A and B enzyme immunoassay for laboratory detection of Clostridium difficile?. J. Clin. Microbiol. 2008, 46:1523-1525.
7. Goldenberg S.D., Cliff P.R., French G.L. Glutamate dehydrogenase for laboratory diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 2010, 48:3050-3051.
8. Karre T., Sloan L., Patel R., Mandrekar J., Rosenblatt J. Comparison of two commercial molecular assays to a laboratory developed molecular assays for diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 2011, 49:725-727.
9. Kim J., Smathers S.A., Prasad P., Leckerman K.H., Coffin S., Zaoutis T. Epidemiological features of Clostridium difficile-associated disease among inpatients at children's hospitals in the United States, 2001-2006. Pediatrics 2008, 122:1266-1270.
10. Kvach E.J., Ferguson D., Riska P.F., Landry M.L. Comparison of BD GeneOhm Cdiff real-time PCR assay with a two-step algorithm and a toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection. J. Clin. Microbiol. 2010, 48:109-114.
11. Larson A.M., Fung A.M., Fang F.C. Evaluation of tcdB real-time PCR in a three-step diagnostic algorithm for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 2010, 48:124-130.
12. Luna R.A., Boyanton B.L., Mehta S., Courtney E.M., Webb C.R., Revell P.A., Versalovic J. Rapid stool-based detection of Clostridium difficile infection by real-time PCR in a children's hospital. J. Clin. Microbiol. 2011, 49:851-857.
13. Novak-Weekley S.M., Marlowe E.M., Miller J.M., Cumpio J., Nomura J.H., Vance P.H., Weissfeld A. Clostridium difficile testing in the clinical laboratory by use of multiple testing algorithms. J. Clin. Microbiol. 2010, 48:889-893.
14. Peterson L.R., Robicsek A. Does my patient have Clostridium difficile infection?. Ann. Intern. Med. 2009, 151:176-179.
15. Quinn C.D., Sefers S.E., Babiker W., He Y., Alcabasa R., Stratton C.W., Carroll K.C., Tang Y.W. C. Diff Quik Chek Complete EIA provides a reliable first-line method for detection of Clostridium difficile in stool specimens. J. Clin. Microbiol. 2010, 48:603-605.
16. Reller M.E., Lema C.A., Perl T.M., Cai M., Ross T.L., Spek K.A., Carroll K.C. Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 2007, 45:3601-3605.
17. Reller M.E., Alcabasa R.C., Lema C.A., Carroll K.C. Comparison of two rapid assays for Clostridium difficile common antigen and a C. difficile toxin A/B assay with the cell culture neutralization assay. Am. J. Clin. Pathol. 2010, 133:107-109.
18. Sharp S.E., Ruben L.O., Pohl J.C., Hatcher P.A., Jayne L.M., Ivie W.M. Evaluation of the C. Diff Quik Chek Complete assay, a new GDH & A/B toxin combination lateral flow assay for use in the rapid, simple diagnosis of Clostridium difficile disease. J. Clin. Microbiol. 2010, 48:2082-2086.
19. Sloan L.M., Duresko B.J., Gustafson D.R., Rosenblatt J.E. Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 2008, 46:1996-2001.
20. Stamper P.D., Alcabasa R., Aird D., Babiker W., Wehrlin J., Ikpeama I., Carroll K.C. Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples. J. Clin. Microbiol. 2009, 47:373-378.
21. Stamper P.D., Babiker W., Alcabasa R., Aird D., Wehrlin J., Ikpeama I., Gluck L., Carroll K.C. Evaluation of a new commercial TaqMan PCR assay for direct detection of Clostridium difficile toxin B gene in clinical stool specimens. J. Clin. Microbiol. 2009, 47:3846-3850.
22. Swindells J., Brenwald N., Reading N., Oppenheim B. Evaluation of diagnostic tests for Clostridium difficile infection. J. Clin. Microbiol. 2010, 48:606-608.
23. Tenover F.C., Novak-Weekley S., Woods C.W., Peterson L.R., Davis T., Schreckenberger P., Fang F.C., Dascal A., Gerding D.N., Nomura J.H., Goering R.V., Akerlund T., Weissfeld A.S., Baron E.J., Wong E., Marlowe E.M., Whitmore J., Persing D.H. Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches. J. Clin. Microbiol. 2010, 48:3719-3724.
24. Ticehurst J.R., Aird D.Z., Dam L.M., Borek A.P., Hargrove J.T., Carroll K.C. Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J. Clin. Microbiol. 2006, 44:1145-1149.
25. Wilcox M.H., Planche T., Fang F.C., Gilligan P. Point-counterpoint. What is the current role of algorithmic approaches for diagnosis of Clostridium difficile infection?. J. Clin. Microbiol. 2010, 48:4347-4353.
26. Zilberberg M.D., Tillotson G.S., McDonald L.C. Clostridium difficile infections among hospitalized children, United States, 1997-2006. Emerg. Infect. Dis. 2010, 16:604-609.