A western blot assay to measure cyclin dependent kinase activity in cells or in vitro without the use of radioisotopes

FEBS Letters

Volumn 587, Issue 18, 2013, Pages 3089-3095

  Lewis, Cody W ^a   Taylor, Ryan G ^a   Kubara, Philip M ^a   Marshall, Kris ^a   Meijer, Laurent ^b   Golsteyn, Roy M ^a  

^a UNIVERSITY OF LETHBRIDGE   (Canada)

^b Centre de Perharidy   (France)

Author keywords

Cdk1; Mitosis; Protein phosphatase 1C ; Tissue culture

Indexed keywords

ADENOSINE TRIPHOSPHATE; CYCLIN DEPENDENT KINASE; FLUOROCHROME; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN PHOSPHATASE 1C ALPHA; RADIOISOTOPE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IN VITRO STUDY; INTERPHASE; MITOSIS; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; WESTERN BLOTTING;

4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID; CAMPTOTHECIN; CDK1; CDK2; CPT; CYCLIN DEPENDENT KINASE 1; CYCLIN DEPENDENT KINASE 2; DIMETHYL SULFOXIDE; DITHIOTHREITOL; DMSO; DTT; EGTA; ETHYLENEGLYCOL-BIS (BETA-AMINOETHYLETHER)- N,N′-TETRAACETIC ACID; HEPES; IPTG; ISOPROPYL Β-D-1-THIOGALACTOPYRANOSIDE; MITOSIS; PBS; PHOSPHATE-BUFFERED SALINE; PROTEIN PHOSPHATASE 1CΑ; TISSUE CULTURE;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CDC2 PROTEIN KINASE; ESCHERICHIA COLI; GENE EXPRESSION; HT29 CELLS; HUMANS; MCF-7 CELLS; MITOSIS; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN PHOSPHATASE 1; RECOMBINANT FUSION PROTEINS;

EID: 84883488396     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2013.08.003     Document Type: Article

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