Absolute quantitation of endogenous proteins with precision and accuracy using a capillary Western system

Analytical Biochemistry

Volumn 442, Issue 1, 2013, Pages 97-103

  Chen, Jin Qiu ^a   Heldman, Madeleine R ^a   Herrmann, Michelle A ^a   Kedei, Noemi ^a   Woo, Wonhee ^a   Blumberg, Peter M ^a   Goldsmith, Paul K ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Capillary immunoassay; Protein kinase C (PKC); Quantitative protein analysis; Simple Western

Indexed keywords

ANTIBODIES; CALCIUM COMPOUNDS; CELL CULTURE; ENZYMES; IMMUNOLOGY;

ACCURATE QUANTIFICATIONS; BIOLOGICAL RESPONSE; CAPILLARY IMMUNOASSAY; PROTEIN ANALYSIS; PROTEIN EXPRESSION LEVELS; PROTEIN EXPRESSIONS; PROTEIN KINASE C; SIMPLE WESTERN;

CELLS;

ANTIBODY; BRYOSTATIN 1; EPITOPE; LIGAND; PROTEIN; PROTEIN KINASE C; ISOENZYME; RECOMBINANT PROTEIN;

ACCURACY; ANALYTIC METHOD; ANTIBODY AFFINITY; ANTINEOPLASTIC ACTIVITY; ARTICLE; AUTOMATION; CAPILLARY IMMUNOASSAY; CELL LINE; CELL LYSATE; CELL STRAIN LNCAP; CELL STRAIN U 937; CELL STRAIN U937; CONTROLLED STUDY; DRUG TARGETING; HUMAN; HUMAN CELL; IMMUNOASSAY; LIMIT OF QUANTITATION; MEASUREMENT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN TARGETING; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIMPLE WESTERN SYSTEM; WESTERN BLOTTING; METABOLISM; PROCEDURES; TUMOR CELL LINE;

AUTOMATION; BLOTTING, WESTERN; CELL LINE, TUMOR; HUMANS; IMMUNOASSAY; ISOENZYMES; PROTEIN KINASE C; RECOMBINANT PROTEINS;

EID: 84883461647     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.07.022     Document Type: Article

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