Vero cells expressing porcine circovirus type 2-capsid protein and their diagnostic application

Journal of Virological Methods

Volumn 194, Issue 1-2, 2013, Pages 26-32

  Kim, Yeon Hee ^a,b   Kweon, Chang Hee ^a   Kang, Seung Won ^a   Oh, Yoon I ^a   Song, Jae Young ^a   Lee, Kyoung Ki ^a   Park, Se Chang ^b  

^a Animal Plant and Fisheries Quarantine and Inspection Agency   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

Boar; Lentiviral transduction; Porcine circovirus type 2; Quantitative IFA

Indexed keywords

ANTIGEN; LENTIVIRUS VECTOR; NUCLEOCAPSID PROTEIN; RECOMBINANT NUCLEOCAPSID PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS ANTIBODY;

ANIMAL CELL; ANTIBODY DETECTION; ARTICLE; BONE MARROW CELL; CIRCOVIRUS; CONTROLLED STUDY; DIAGNOSTIC TEST; DIAGNOSTIC TEST ACCURACY STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES; FEMALE; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PERFORMANCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE IMMUNOFLUORESCENCE ASSAY; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY; SERUM; SPERM; SWINE; VERO CELL; VIRAL DISEASE IMMUNOFLUORESCENCE ASSAY; VIRUS GENOME; VIRUS LOAD;

BOAR; LENTIVIRAL TRANSDUCTION; PORCINE CIRCOVIRUS TYPE 2; QUANTITATIVE IFA;

ANIMALS; ANTIBODIES, VIRAL; CAPSID PROTEINS; CERCOPITHECUS AETHIOPS; CIRCOVIRUS; DIAGNOSTIC TESTS, ROUTINE; FLUORESCENT ANTIBODY TECHNIQUE; PORCINE POSTWEANING MULTISYSTEMIC WASTING SYNDROME; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SWINE; VERO CELLS; VETERINARY MEDICINE;

EID: 84883366693     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.07.030     Document Type: Article

References (37)

1. Alberti K.A., Estienne M.J., Meng X.J. Effect of vaccination of boars against porcine circovirus type 2 on ejaculate characteristics, serum antibody titers, viremia, and semen virus shedding. J. Anim. Sci. 2011, 89:1581-1587.
2. Allan G.M., McNeilly F., Kennedy S., Daft B., Clarke E.G., Ellis J.A., Haines D.M., Meehan B.M., Adair B.M. Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. J. Vet. Diagn. Invest. 1998, 10:3-10.
3. Blanchard P., Mahe D., Cariolet R., Truong C., Le Dimna M., Arnauld C., Rose N., Eveno E., Albina E., Madec F., Jestin A. An ORF2 protein-based ELISA for porcine circovirus type 2 antibodies in post-weaning multisystemic wasting syndrome. Vet. Microbiol. 2003, 94:183-194.
4. Brunborg I.M., Moldal T., Jonassen C.M. Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting syndrome using a TaqMan-based real-time PCR. J. Virol. Methods 2004, 122:171-178.
5. Chae C. A review of porcine circovirus 2-associated syndromes and diseases. Vet. J. 2005, 169:326-336.
6. Chae C. Porcine circovirus type 2 and its associated diseases in Korea. Virus Res. 2012, 164:107-113.
7. Cooray S., Howe S.J., Thrasher A.J. Retrovirus and lentivirus vector design and methods of cell conditioning. Methods Enzymol. 2012, 507:29-57.
8. Ellis J., Hassard L., Clark E., Harding J., Allan G., Willson P., Strokappe J., Martin K., McNeilly F., Meehan B., Todd D., Haines D. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can. Vet. J. 1998, 39:44-51.
9. Follenzi A., Ailles L.E., Bakovic S., Geuna M., Naldini L. Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences. Nat. Genet. 2000, 25:217-222.
10. Fort M., Olvera A., Sibila M., Segales J., Mateu E. Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs. Vet. Microbiol. 2007, 125:244-255.
11. Huang L., Lu Y., Wei Y., Guo L., Liu C. Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2. J. Virol. Methods 2011, 171:26-33.
12. Jianguo W., Tianhang L., Hong Z., Zhengmao L., Jianwei B., Xuchao X., Guoen F. Optimization of culture conditions for endothelial progenitor cells from porcine bone marrow in vitro. Cell Prolif. 2010, 43:418-426.
13. Kweon C.H., Kwon B.J., Kim I.J., Lee S.Y., Ko Y.J. Development of monoclonal antibody-linked ELISA for sero-diagnosis of vesicular stomatitis virus (VSV-IN) using baculovirus expressed glycoprotein. J. Virol. Methods 2005, 130:7-14.
14. Kweon C.H., Oh Y.I., Song J.Y., Lee K.K. Indirect ELISA for the Detection of Antibodies to Porcine Circovirus Type 2 (PCV2). Proc. Korean J. Vet. Sci. 2011, 5(3):292.
15. Liu C., Ihara T., Nunoya T., Ueda S. Development of an ELISA based on the baculovirus-expressed capsid protein of porcine circovirus type 2 as antigen. J. Vet. Med. Sci. 2004, 66:237-242.
16. Madson D.M., Ramamoorthy S., Kuster C., Pal N., Meng X.J., Halbur P.G., Opriessnig T. Characterization of shedding patterns of Porcine circovirus types 2a and 2b in esperimentally inoculated mature boars. J. Vet. Diagn. Invest. 2008, 20:725-734.
17. Madson D.M., Ramamoorthy S., Kuster C., Pal N., Meng X.J., Halbur P.G., Opriessnig T. Infectivity of porcine circovirus type 2 DNA in semen from experimentally-infected boars. Vet. Res. 2009, 40:10.
18. Magar R., Muller P., Larochelle R. Retrospective serological survey of antibodies to porcine circovirus type 1 and type 2. Can. J. Vet. Res. 2000, 64:184-186.
19. Mahe D., Blanchard P., Truong C., Arnauld C., Le Cann P., Cariolet R., Madec F., Albina E., Jestin A. Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes. J. Gen. Virol. 2000, 81:1815-1824.
20. Mcintosh K.A., Harding J.C., Parker S., Ellis J.A., Appleyard G.D. Nested polymerase chain reaction detection and duration of porcine circovirus type 2 in semen with sperm morphological analysis from naturally infected boars. J. Vet. Diagn. Invest. 2006, 18:380-384.
21. Morozov I., Sirinarumitr T., Sorden S.D., Halbur P.G., Morgan M.K., Yoon K.J., Paul P.S. Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J. Clin. Microbiol. 1998, 36:2535-2541.
22. Nawagitgul P., Harms P.A., Morozov I., Thacker B.J., Sorden S.D., Lekcharoensuk C., Paul P.S. Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin. Diagn. Lab. Immunol. 2002, 9:33-40.
23. Nawagitgul P., Morozov I., Bolin S.R., Harms P.A., Sorden S.D., Paul P.S. Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J. Gen. Virol. 2000, 81:2281-2287.
24. Opriessnig T., Meng X.J., Halbur P.G. Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J. Vet. Diagn. Invest. 2007, 19:591-615.
25. Perez-Martin E., Grau-Roma L., Argilaguet J.M., Nofrarias M., Escribano J.M., Gomez-Sebastian S., Segales J., Rodriguez F. Development of two Trichoplusia ni larvae-derived ELISAs for the detection of antibodies against replicase and capsid proteins of porcine circovirus type 2 in domestic pigs. J. Virol. Methods 2008, 154:167-174.
26. Prieto C., Fontana D., Etcheverrigaray M., Kratje R. A strategy to obtain recombinant cell lines with high expression levels Lentiviral vector-mediated transgenesis. BMC Proc. 2011, 5(Suppl. 8):P7.
27. Racine S., Kheyar A., Gagnon C.A., Charbonneau B., Dea S. Eucaryotic expression of the nucleocapsid protein gene of porcine circovirus type 2 and use of the protein in an indirect immunofluorescence assay for serological diagnosis of postweaning multisystemic wasting syndrome in pigs. Clin. Diagn. Lab. Immunol. 2004, 11:736-741.
28. Roh I.S., Lee K.W., Kim J.H., Han J.H., Jean Y.H. Pathogenesis and pathogenicity for the porcine circovirus 2 Korean isolates in weaned pigs. Korean J. Vet. Res. 2007, 47:175-185.
29. Segales J., Allan G.M., Domingo M. Porcine circovirus diseases. Anim. Health Res. Rev. 2005, 6:119-142.
30. Segales J., Sitjar M., Domingo M., Dee S., Del Pozo M., Noval R., Sacristan C., De las Heras A., Ferro A., Latimer K.S. First report of post-weaning multisystemic wasting syndrome in pigs in Spain. Vet. Rec. 1997, 141:600-601.
31. Segura M.M., Garnier A., Durocher Y., Coelho H., Kamen A. Production of lentiviral vectors by large-scale transient transfection of suspension cultures and affinity chromatography purification. Biotechnol. Bioeng. 2007, 98:789-799.
32. Shang S.B., Li Y.F., Guo J.Q., Wang Z.T., Chen Q.X., Shen H.G., Zhou J.Y. Development and validation of a recombinant capsid protein-based ELISA for detection of antibody to porcine circovirus type 2. Res. Vet. Sci. 2008, 84:150-157.
33. Tischer I., Gelderblom H., Vettermann W., Koch M.A. A very small porcine virus with circular single-stranded DNA. Nature 1982, 295:64-66.
34. Tischer I., Peters D., Rasch R., Pociuli S. Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Arch. Virol. 1987, 96:39-57.
35. Tischer I., Rasch R., Tochtermann G. Characterization of papovavirus- and picornavirus-like particles in permanent pig kidney cell lines. Zentralbl. Bakteriol. Orig. A 1974, 226:153-167.
36. Truong C., Mahe D., Blanchard P., Le Dimna M., Madec F., Jestin A., Albina E. Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection. Arch. Virol. 2001, 146:1197-1211.
37. Walker I.W., Konoby C.A., Jewhurst V.A., McNair I., McNeilly F., Meehan B.M., Cottrell T.S., Ellis J.A., Allan G.M. Development and application of a competitive enzyme-linked immunosorbent assay for the detection of serum antibodies to porcine circovirus type 2. J. Vet. Diagn. Invest. 2000, 12:400-405.