Development of two Trichoplusia ni larvae-derived ELISAs for the detection of antibodies against replicase and capsid proteins of porcine circovirus type 2 in domestic pigs

Journal of Virological Methods

Volumn 154, Issue 1-2, 2008, Pages 167-174

  Pérez Martín, Eva ^a   Grau Roma, Llorenç ^a,b   Argilaguet, Jordi M ^a   Nofrarías, Miquel ^a   Escribano, José M ^c,d   Gómez Sebastián, Silvia ^c   Segalés, Joaquim ^a,b   Rodríguez, Fernando ^a  

^a CENTRE DE RECERCA EN SANITAT ANIMAL CRESA   (Spain)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c UNIVERSIDAD POLITÉCNICA DE MADRID   (Spain)

^d INIA   (Spain)

Author keywords

Capsid protein; ELISA; Porcine circovirus type 2 (PCV2); Postweaning multisystemic wasting syndrome (PMWS); Replicase protein; Trichoplusia ni insect larvae

Indexed keywords

CAPSID PROTEIN; INSECT PROTEIN; REPLICASE PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY BLOOD LEVEL; ANTIBODY DETECTION; ARTHROPOD LARVA; ARTICLE; AUTOPSY; BACULOVIRUS; CIRCOVIRIDAE; CONTROLLED STUDY; DOMESTIC PIG; ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; OUTCOME ASSESSMENT; POSTWEANING MULTISYSTEMIC WASTING SYNDROME; PRIORITY JOURNAL; PROTEIN EXPRESSION; VIRUS CAPSID; VIRUS INFECTION;

ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; BACULOVIRIDAE; CAPSID PROTEINS; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; DNA-DIRECTED DNA POLYMERASE; ENZYME-LINKED IMMUNOSORBENT ASSAY; LARVA; LEPIDOPTERA; SUS SCROFA;

ANIMALIA; HEXAPODA; MIRIDAE; PORCINE CIRCOVIRUS 2; SUIDAE; SUS SCROFA DOMESTICA; TRICHOPLUSIA NI;

EID: 56249098617     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.07.034     Document Type: Article

References (49)

1. Allan G.M., McNeilly F., Meehan B.M., Kennedy S., Mackie D.P., Ellis J.A., Clark E.G., Espuña E., Saubi N., Riera P., Botner A., and Charreyre C.E. Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Vet. Microbiol. 66 (1999) 115-123
2. Barderas M.G., Wigdorovitz A., Merelo F., Beitia F., Alonso C., Borca M.V., and Escribano J.M. Serodiagnosis of African swine fever using the recombinant protein p30 expressed in insect larvae. J. Virol. Methods 89 (2000) 129-136
3. Barderas M.G., Rodriguez F., Gomez-Puertas P., Aviles M., Beitia F., Alonso C., and Escribano J.M. Antigenic and immunogenic properties of a chimera of two immunodominant African swine fever virus proteins. Arch. Virol. 146 (2001) 1681-1691
4. Blanchard P., Mahe D., Cariolet R., Truong C., Le Dimna M., Arnauld C., Rose N., Eveno E., Albina E., Madec F., and Jestin A. An ORF2 protein-based ELISA for porcine circovirus type 2 antibodies in postweaning multisystemic wasting syndrome. Vet. Microbiol. 94 (2003) 183-194
5. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
6. Brunborg I.M., Moldal T., and Jonassen C.M. Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting syndrome using a TaqMan-based real-time PCR. J. Virol. Methods 122 (2004) 171-178
7. Calsamiglia M., Segalés J., Quintana J., Rosell C., and Domingo M. Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome. J. Clin. Microbiol. 40 (2002) 1848-1850
8. Carasova P., Celer V., Takacova K., Trundova M., Molinkova D., Lobova D., and Smola J. The levels of PCV2 specific antibodies and viremia in pigs. Res. Vet. Sci. 83 (2007) 274-278
9. Cheung A.K., and Bolin S.R. Kinetics of porcine circovirus type 2 replication. Arch. Virol. 147 (2002) 43-58
10. Ellis J., Hassard L., Clark E., Harding J., Allan G., Willson P., Strokappe J., Martin K., McNeilly F., Meehan B., Todd D., and Haines D. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can. Vet. J. 39 (1998) 44-51
11. Fachinger V., Bischoff R., Jedidia S.B., Saalmüller A., and Elbers K. The effect of vaccination against porcine circovirus type 2 in pigs suffering from porcine respiratory disease complex. Vaccine 26 (2008) 1488-1499
12. Fort M., Olvera A., Sibila M., Segalés J., and Mateu E. Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs. Vet. Microbiol. 125 (2007) 244-255
13. Fort M., Sibila M., Allepuz A., Mateu E., Roerink F., and Segalés J. Porcine circovirus type 2 (PCV2) vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins. Vaccine 26 (2008) 1063-1071
14. Grau-Roma L., Crisci E., Sibila M., López-Soria S., Nofrarias M., Cortey M., Fraile L., Olvera A., and Segalés J. A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence. Vet. Microbiol. 1 128 (2008) 23-35
15. Hamel A.L., Lin L.L., and Nayar G.P. Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. J. Virol. 72 (1998) 5262-5267
16. Larochelle R., Magar R., and D'Allaire S. Comparative serologic and virologic study of commercial swine herds with and without postweaning multisystemic wasting syndrome. Can. J. Vet. Res. 67 (2003) 114-120
17. Liu C., Ihara T., Nunoya T., and Ueda S. Development of an ELISA based on the baculovirus-expressed capsid protein of porcine circovirus type 2 as antigen. J. Vet. Med. Sci. 66 (2004) 237-242
18. Liu J., Zhu Y., Chen I., Lau J., He F., Lau A., Wang Z., Karuppannan A.K., and Kwang J. The ORF3 protein of porcine circovirus type 2 interacts with porcine ubiquitin E3 ligase Pirh2 and facilitates p53 expression in viral infection. J. Virol. 123 (2007) 203-209
19. Liu Q., Tikoo S.K., and Babiuk L.A. Nuclear localization of the ORF2 protein encoded by porcine circovirus Type 2. Virology 285 (2001) 91-99
20. Magar R., Müller P., and Larochelle R. Retrospective serological survey of antibodies to porcine circovirus type 1 and type 2. Can. J. Vet. Res. 64 (2000) 184-186
21. Mahé D., Blanchard P., Truong C., Arnauld C., Le Cann P., Cariolet R., Madec F., Albina E., and Jestin A. Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes. J. Gen. Virol. 81 (2000) 1815-1824
22. Mankertz A., Caliskan R., Hattermann K., Hillenbrand B., Krzendoerfer P., Mueller B., Schmitt C., Steinfeldt T., and Finsterbusch T. Molecular biology of porcine circovirus: analyses of gene expression and viral replication. Vet. Microbiol. 98 (2004) 81-88
23. Mankertz A., Domingo M., Folch J.M., LeCann P., Jestin A., Segalés J., Chmielewicz B., Plana-Duran J., and Soike D. Characterisation of PCV-2 isolates form Spain, Germany and France. Virus Res. 66 (2000) 65-77
24. Mankertz A., Mankertz J., Wolf K., and Buhk H.J. Identification of a protein essential for replication of porcine circovirus. J. Gen. Virol. 79 (1998) 381-384
25. Medin J.A., Hunt L., Gathy K., Evans R.K., and Coleman M.S. Efficient, low-cost protein factories: expression of human adenosine deaminase in baculovirus-infected insect larvae. Proc. Natl. Acad. Sci. U.S.A. 87 (1990) 2760-2764
26. Medin J.A., Gathy K., and Coleman M.S. Expression of foreign proteins in Trichoplusia ni larvae. In: Richardson C.D. (Ed). Methods in Molecular Biology: Baculovirus Expression Protocols vol. 39 (1995), Humana Press, Totowa, NJ 265-275
27. Meehan B.M., McNeilly F., Todd D., Kennedy S., Jewhurst V.A., Ellis J.A., Hassard L.E., Clark E.G., Haines D.M., and Allan G.M. Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. J. Gen. Virol. 79 (1998) 2171-2179
28. Meerts P., Misinzo G., Lefebvre D., Nielsen J., Botner A., Kristensen C.S., and Nauwynck H.J. Correlation between the presence of neutralizing antibodies against porcine circovirus 2 (PCV2) and protection against replication of the virus and development of PCV2-associated disease. BMC Vet. Res. 2 (2006) 6
29. Nawagitgul P., Harms P.A., Morozov I., Thacker B.J., Sorden S.D., Lekcharoensuk C., and Paul P.S. Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin. Diagn. Lab. Immunol. 9 (2002) 33-40
30. Olvera A., Sibila M., Calsamiglia M., Segales J., and Domingo M. Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs. J. Virol. Methods 117 (2004) 75-80
31. Opriessnig T., Patterson A.R., Elsener J., Meng X.J., and Halbur P.G. Influence of maternal antibodies on efficacy of porcine circovirus type 2 (PCV2) vaccination to protect pigs from experimental infection with PCV2. Clin. Vaccine Immunol. 15 (2008) 397-401
32. Pérez-Filgueira D.M., Gonzalez-Camacho F., Gallardo C., Resino-Talavan P., Blanco E., Gomez-Casado E., Alonso C., and Escribano J.M. Optimization and validation of recombinant serological tests for African Swine Fever diagnosis based on detection of the p30 protein produced in Trichoplusia ni larvae. J. Clin. Microbiol. 44 (2006) 3114-3121
33. Pérez-Filgueira D.M., Resino-Talaván P., Cubillos C., Angulo I., Barderas M.G., Barcena J., and Escribano J.M. Development of a low-cost, insect larvae-derived recombinant subunit vaccine against RHDV. Virology 364 (2007) 422-430
34. Pogranichniy R.M., Yoon K.J., Harms P.A., Sorden S.D., and Daniels M. Case-control study on the association of porcine circovirus type 2 and other swine viral pathogens with postweaning multisystemic wasting syndrome. J. Vet. Diagn. Invest. 14 (2002) 449-456
35. Ramírez-Mendoza H., Martínez C., Mercado C., Castillo-Juárez H., Hernández J., and Segalés J. Porcine circovirus type 2 antibody detection in backyard pigs from Mexico City. Res. Vet. Sci. 83 (2007) 130-132
36. Reis U., Blum B., von Specht B.U., Domdey H., and Collins J. Antibody production in silkworm cells and silkworm larvae infected with a dual recombinant Bombyx mori nuclear polyhedrosis virus. Biotechnology (NY) 10 (1992) 910-912
37. Roca M., Balasch M., Segalés J., Calsamiglia M., Viaplana E., Urniza A., Hattermann K., Mankertz A., Plana-Duran J., and Domingo M. In vitro and in vivo characterization of an infectious clone of a European strain of porcine circovirus type 2. J. Gen. Virol. 85 (2004) 1259-1266
38. Rodríguez-Arrioja G.M., Segalés J., Balasch M., Rosell C., Quintana J., Folch J.M., Plana-Durán J., Mankertz A., and Domingo M. Serum antibodies to porcine circovirus type 1 (PCV-1) and type 2 (PCV-2) in pigs with and without postweaning multisystemic wasting syndrome (PMWS). Vet. Rec. 146 (2000) 762-764
39. Segalés J. Update on postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome diagnostics. J. Swine Health Prod. 10 (2002) 277-281
40. Segalés J., Allan M.G., and Domingo M. Porcine circovirus diseases. Anim. Health Res. Rev. 6 (2005) 119-142
41. Segalés J., and Domingo M. Postweaning multysistemic wasting syndrome (PMWS) in pigs. A review. Vet. Q. 24 (2002) 109-124
42. Sibila M., Calsamiglia M., Segalés J., Blanchard P., Badiella L., Le Dimna M., Jestin A., and Domingo M. Use of a polymerase chain reaction assay and an ELISA to monitor porcine circovirus type 2 infection in pigs from farms with and without postweaning multisystemic wasting syndrome. Am. J. Vet. Res. 65 (2004) 88-92
43. Sorden S.D. Update on porcine circovirus and postweaning multisystemic wasting syndrome. J. Swine Health Prod. 8 (2000) 133-136
44. Shang S.B., Li Y.F., Guo J.Q., Wang Z.T., Chen Q.X., Shen H.G., and Zhou J.Y. Development and validation of a recombinant capsid protein-based ELISA for detection of antibody to porcine circovirus type 2. Res. Vet. Sci. 84 (2008) 150-157
45. Sumathy S., Palhan V.B., and Gopinathan K.P. Expression of human growth hormone in silkworm larvae through recombinant Bombyx mori nuclear polyhedrosis virus. Protein Exp. Purif. 7 (1996) 262-268
46. Todd, D., McNulty, M.S., Mankertz, A., Luckert, P.D., Randles, J.W., Dale, J.L., 2000. Family Circoviridae. In: Van Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L., Carstens, E.B., Estes, K.M., Lemon, S.M., McGeoch, D.J., Maniloff, J., Mayo, M.A., Pringle, C.R., Wickner, R.B. (Eds.). Virus taxonomy: classification and nomenclature of viruses. Seventh report of the International Committee on Taxonomy of Viruses. Academic Press, San Diego, 299-302.
47. Vicente J., Segalés J., Höfle U., Balasch M., Plana-Durán J., Domingo M., and Gortázar C. Epidemiological study on porcine circovirus type 2 (PCV2) infection in the European wild boar (Sus scrofa). Vet. Res. 35 (2004) 243-253
48. Walker I.W., Konoby C.A., Jewhurst V.A., McNair I., McNeilly F., Meehan B.M., Cottrell T.S., Ellis J.A., and Allan G.M. Development and application of a competitive enzyme-linked immunosorbent assay for the detection of serum antibodies to porcine circovirus type 2. J. Vet. Diagn. Invest. 12 (2000) 400-405
49. Wu P.C., Chien M.S., Tseng Y.Y., Lin J., Lin W.L., Yang C.Y., and Huang C. Expression of the porcine circovirus type 2 capsid protein subunits and application to an indirect ELISA. J. Biotechnol. 133 (2008) 58-64