Formation of microvascular networks in vitro

Nature Protocols

Volumn 8, Issue 9, 2013, Pages 1820-1836

  Morgan, John P ^a   Delnero, Peter F ^a   Zheng, Ying ^b   Verbridge, Scott S ^a,g   Chen, Junmei ^c   Craven, Michael ^a,g,h   Choi, Nak Won ^a,g,i   Diaz Santana, Anthony ^a,g   Kermani, Pouneh ^d   Hempstead, Barbara ^d   López, José A ^c,e   Corso, Thomas N ^f   Fischbach, Claudia ^a   Stroock, Abraham D ^a,j  

^a Cornell University   (United States)

^b University of Washington   (United States)

^c PUGET SOUND BLOOD CENTER   (United States)

^d WEILL CORNELL MEDICAL COLLEGE   (United States)

^e UNIVERSITY OF WASHINGTON   (United States)

^f Virginia Polytechnic Institute ^*  (United States)

^g VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^h IFyber LLC   (United States)

ⁱ Korea Institute of Science and Technology   (South Korea)

^j L ORÉAL RECHERCHE   (France)

more..

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN TYPE 1;

ARTICLE; CELL CULTURE; COCULTURE; ENDOTHELIUM CELL; FLUORESCENCE IMAGING; FORCE; HEMODYNAMICS; HISTOLOGY; HUMAN; HUMAN CELL; HYDROGEL; IN VITRO STUDY; MICROFLUIDICS; MICROTECHNOLOGY; MICROVASCULATURE; PARENCHYMA; PERFUSION; PRIORITY JOURNAL; REGENERATIVE MEDICINE; TISSUE ENGINEERING; TRANSMISSION ELECTRON MICROSCOPY; TUMOR THROMBUS; TUMOR VASCULARIZATION;

CELL CULTURE TECHNIQUES; CELLS, CULTURED; COCULTURE TECHNIQUES; COLLAGEN TYPE I; ENDOTHELIAL CELLS; HUMANS; HYDROGEL; MICROSCOPY, ELECTRON, TRANSMISSION; MICROTECHNOLOGY; MICROVESSELS; NEOVASCULARIZATION, PHYSIOLOGIC; OPTICAL IMAGING; TISSUE ENGINEERING;

EID: 84883342307     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.110     Document Type: Article

References (47)

1. Choi, N.W. et al. Microfluidic scaffolds for tissue engineering. Nat. Mater. 6, 908-915 (2007).
2. Zheng, Y. et al. In vitro microvessels for the study of angiogenesis and thrombosis. Proc. Natl. Acad. Sci. USA 109, 9342-9347 (2012).
3. Verbridge, S.S. et al. Physicochemical regulation of endothelial sprouting in a 3-D microfluidic angiogenesis model. J. Biomed. Mater. Res. A doi:10.1002/jbm.a.34587 (5 April 2013).
4. Stroock, A.D. & Fischbach, C. Microfluidic culture models of tumor angiogenesis. Tissue Eng. Part A 16, 2143-2146 (2010).
5. Verbridge, S.S. et al. Oxygen-controlled 3-D cultures to analyze tumor angiogenesis. Tissue Eng Part A 16, 2157-2159 (2010).
6. Song, J.W. & Munn, L.L. Fluid forces control endothelial sprouting. Proc. Natl. Acad. Sci. USA 108, 15342-15347 (2011).
7. Song, J.W., Bazou, D. & Munn, L.L. Anastomosis of endothelial sprouts forms new vessels in a tissue analogue of angiogenesis. Integr. Biol. 4, 857-862 (2012).
8. Shin, Y. et al. In vitro 3D collective sprouting angiogenesis under orchestrated ANG-1 and VEGF gradients. Lab Chip 11, 2175-2181 (2011).
9. Shin, Y. et al. Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels. Nat. Protoc. 7, 1247-1259 (2012).
10. Davis, G.E., Bayless, K.J. & Mavila, A. Molecular basis of endothelial cell morphogenesis in three-dimensional extracellular matrices. Anat. Rec. 268, 252-275 (2002).
11. Yannas, I.V. & Burke, J.F. Design of an artificial skin I. basic design principles. J. Biomed. Mater. Res. 14, 65-81 (1980).
12. Langer, R. & Vacanti, J.P. Tissue engineering. Science 260, 920-926 (1993).
13. Jain, R.K., Au, P., Tam, J., Duda, D.G. & Fukumura, D. Engineering vascularized tissue. Nat. Biotechnol. 23, 821-823 (2005).
14. Stroock, A.D. & Cabodi, M. Microfluidic biomaterials. MRS Bulletin 31, 114-119 (2006).
15. Mikos, A.G. et al. Prevascularization of porous biodegradable polymers. Biotechnol. Bioeng. 42, 716-723 (1993).
16. Levenberg, S. et al. Engineering vascularized skeletal muscle tissue. Nat. Biotechnol. 23, 879-884 (2005).
17. Du, Y. et al. Sequential assembly of cell-laden hydrogel constructs to engineer vascular-like microchannels. Biotechnol. Bioeng. 108, 1693-1703 (2011).
18. Neumann, T., Nicholson, B.S. & Sanders, J.E. Tissue engineering of perfused microvessels. Microvasc. Res. 66, 59-67 (2003).
19. Cabodi, M. et al. A microfluidic biomaterial. J. Am. Chem. Soc. 127, 13788-13789 (2005).
20. Chrobak, K.M., Potter, D.R. & Tien, J. Formation of perfused, functional microvascular tubes in vitro. Microvasc. Res. 71, 185-196 (2006).
21. Miller, J.S. et al. Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues. Nat. Mater. 11, 768-774 (2012).
22. Whitesides, G.M., Ostuni, E., Takayama, S., Jiang, X. & Ingber, D.E. Soft lithography in biology and biochemistry. Annu. Rev. Biomed. Eng. 3, 335-373 (2001).
23. Qin, D., Xia, Y. & Whitesides, G.M. Soft lithography for micro- and nanoscale patterning. Nat. Protoc. 5, 491-502 (2010).
24. Tang, M.D., Golden, A.P. & Tien, J. Molding of three-dimensional microstructures of gels. J. Am. Chem. Soc. 125, 12988-12989 (2003).
25. Nelson, C.M., Vanduijn, M.M., Inman, J.L., Fletcher, D.A. & Bissell, M.J. Tissue geometry determines sites of mammary branching morphogenesis in organotypic cultures. Science 314, 298-300 (2006).
26. Bellan, L.M. et al. Fabrication of an artificial 3-dimensional vascular network using sacrificial sugar structures. Soft Matter 5, 1354-1357 (2009).
27. McGuigan, A.P. & Sefton, M.V. Vascularized organoid engineered by modular assembly enables blood perfusion. Proc. Natl. Acad. Sci. USA 103, 11461-11466 (2006).
28. Gauvin, R., Guillemette, M., Dokmeci, M. & Khademhosseini, A. Application of microtechnologies for the vascularization of engineered tissues. Vasc. Cell 3, 24 (2011).
29. Borenstein, J.T. et al. Microfabrication technology for vascularized tissue engineering. Biomed. Microdevices 4, 167-175 (2002).
30. Fidkowski, C. et al. Endothelialized microvasculature based on a biodegradable elastomer. Tissue Eng. 11, 302-309 (2005).
31. Bettinger, C.J. et al. Silk fibroin microfluidic devices. Adv. Mater. 19, 2847-2850 (2007).
32. Ling, Y. et al. A cell-laden microfluidic hydrogel. Lab Chip 7, 756-762 (2007).
33. Nelson, C.M. & Tien, J. Microstructured extracellular matrices in tissue engineering and development. Curr. Opin. Biotechnol. 17, 518-523 (2006).
34. Golden, A.P. & Tien, J. Fabrication of microfluidic hydrogels using molded gelatin as a sacrificial element. Lab Chip 7, 720-725 (2007).
35. Iruela-Arispe, M.L. & Davis, G.E. Cellular and molecular mechanisms of vascular lumen formation. Dev. Cell 16, 222-231 (2009).
36. Asahara, T., Kawamoto, A. & Masuda, H. Concise review: circulating endothelial progenitor cells for vascular medicine. Stem Cells 29, 1650-1655 (2011).
37. Feener, E.P. & King, G.L. Vascular dysfunction in diabetes mellitus. Lancet 350 (suppl. 1), SI9-S13 (1997).
38. Muschler, G.F., Nakamoto, C. & Griffith, L.G. Engineering principles of clinical cell-based tissue engineering. J Bone Joint Surg. Am. 86-A, 1541-1558 (2004).
39. Sung, J.H., Kam, C. & Shuler, M.L. A microfluidic device for a pharmacokinetic-pharmacodynamic (PK-PD) model on a chip. Lab Chip 10, 446-455 (2010).
40. Wong, K.H., Chan, J.M., Kamm, R.D. & Tien, J. Microfluidic models of vascular functions. Annu. Rev. Biomed. Eng. 14, 205-230 (2012).
41. Cross, V.L. et al. Dense collagen matrices with microstructure and cellular remodeling for three-dimensional cell culture. Biomaterials 31, 8596-8607 (2010).
42. King, K.R., Wang, C.C.J., Kaazempur-Mofrad, M.R., Vacanti, J.P. & Borenstein, J.T. Biodegradable microfluidics. Adv. Mater. 16, 2007-2012 (2004).
43. Vickerman, V., Blundo, J., Chung, S. & Kamm, R. Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging. Lab Chip 8, 1468-1477 (2008).
44. Bornstein, M.B. Reconstituted rattail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes. Lab Invest. 7, 134-137 (1958).
45. Rajan, N., Habermehl, J., Cote, M.F., Doillon, C.J. & Mantovani, D. Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications. Nat. Protoc. 1, 2753-2758 (2006).
46. Haessler, U., Pisano, M., Wu, M. & Swartz, M.A. Dendritic cell chemotaxis in 3D under defined chemokine gradients reveals differential response to ligands CCL21 and CCL19. Proc. Natl. Acad. Sci. USA 108, 5614-5619 (2011).
47. Franco, C. & Gerhardt, H. Tissue engineering: blood vessels on a chip. Nature 488, 465-466 (2012).