Carrier free co-immobilization of alpha amylase, glucoamylase and pullulanase as combined cross-linked enzyme aggregates (combi-cleas): a tri-enzyme biocatalyst with one pot starch hydrolytic activity

Bioresource Technology

Volumn 147, Issue , 2013, Pages 269-275

  Talekar, Sachin ^a   Pandharbale, Amol ^b   Ladole, Mayur ^a   Nadar, Shamraja ^a   Mulla, Mosin ^a   Japhalekar, Kshitija ^a   Pattankude, Kishori ^a   Arage, Devika ^a  

^a KIT's College of Engineering   (India)

^b NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Alpha amylase; Combined cross linked enzyme aggregates (combi CLEAs); Glucoamylase; One pot starch hydrolysis; Pullulanase

Indexed keywords

AGGREGATES; AMYLASES; HYDROLYSIS; MIXTURES; SCANNING ELECTRON MICROSCOPY; STARCH;

ALPHA AMYLASE; CROSS-LINKED ENZYME AGGREGATES; GLUCOAMYLASE; PULLULANASE; STARCH HYDROLYSIS;

ENZYME IMMOBILIZATION;

AMYLASE; GLUCAN 1,4 ALPHA GLUCOSIDASE; HYDROLASE; PULLULANASE; STARCH;

CATALYST; ENZYME ACTIVITY; HYDROLYSIS; PH; SCANNING ELECTRON MICROSCOPY; STARCH; TEMPERATURE EFFECT;

ARTICLE; BIOCATALYST; CROSS LINKING; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME STABILITY; HYDROLYSIS; PH; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; THERMOSTABILITY;

ALPHA AMYLASE; COMBINED CROSS-LINKED ENZYME AGGREGATES (COMBI-CLEAS); GLUCOAMYLASE; ONE POT STARCH HYDROLYSIS; PULLULANASE;

ALPHA-AMYLASES; BIOCATALYSIS; ENZYME STABILITY; GLUCAN 1,4-ALPHA-GLUCOSIDASE; GLYCOSIDE HYDROLASES; HYDROLYSIS; TEMPERATURE;

EID: 84883305127     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2013.08.035     Document Type: Article

References (35)

1. Atia K.S., Ismail S.A., El-Arnaouty M.B., Dessouki A.M. Use of coimmobilized β-amylase and pullulanase in reduction of saccharification time of starch and increase in maltose yield. Biotechnol. Prog. 2003, 19:853-857.
2. Bryjak J. Glucoamylase, α-amylase and β-amylase immobilization on acrylic carriers. Biochem. Eng. J. 2003, 16:347-355.
3. Chakrabarti A.C., Storey K.B. Co-immobilization of amyloglucosidase and pullulanase for enhanced starch hydrolysis. Appl. Microbiol. Biotechnol. 1990, 33:48-50.
4. Cordeiro A.L., Lenk T., Werner C. Immobilization of Bacillus licheniformis α-amylase onto reactive polymer films. J. Biotechnol. 2011, 154:216-221.
5. Crabb W.S., Shetty J.K. Commodity scale production of sugars from starches. Curr. Opin. Microbiol. 1999, 2:252-256.
6. Dekker L.A. Worthington Enzyme Manual 1977, Worthington Biochemical Corp, Freehold, NJ, p. 173.
7. Dessouki A.M., Issa G., Atia K.S. Pullulanase immobilization on natural and synthetic polymers. J. Chem. Technol. Biotechnol. 2001, 76:700-706.
8. Eldin M.S.M., Seuror E.I., Nasr M.A., El-Aassar M.R., Tieama H.A. Affinity covalent immobilization of glucoamylase onto rho-benzoquinone activated alginate beads: I beads preparation and characterization. Appl. Biochem. Biotechnol. 2011, 164:10-22.
9. Gupta M.N., Dalal S., Kapoor M. Preparation and characterization of combi-CLEAs catalyzing multiple non cascade reactions. J. Mol. Catal. B Enzym. 2007, 44:128-132.
10. Gupta K., Jana A.K., Kumar S., Maiti M. Immobilization of α-amylase and amyloglucosidase onto ion-exchange resin beads and hydrolysis of natural starch at high concentration. Bioprocess Biosyst. Eng. 2013, (in press).
11. Jung D.H., Jung J.H., Seo D.H., Ha S.J., Kweon D.K., Park C.S. One-pot bioconversion of sucrose to trehalose using enzymatic sequential reactions in combined cross-linked enzyme aggregates. Bioresour. Technol. 2013, 130:801-804.
12. Kimura T., Ogata M., Kobayashi H., Yoshida M., Oishi K., Nakakuki T. Continuous production of maltotetraose using a dual immobilized enzyme system of maltotetraose-forming amylase and pullulanase. Biotechnol. Bioeng. 1990, 36:790-796.
13. Kuroiwa T., Shoda H., Ichikawa S., Sato S., Mukataka S. Immobilization and stabilization of pullulanase from Klebsiella pneumoniae by a multipoint attachment method using activated agar gel supports. Process Biochem. 2005, 40:2637-2642.
14. Majumder A.B., Mondal K., Singh T.P., Gupta M.N. Designing cross-linked lipase aggregates for optimum performance as biocatalysts. Biocatal. Biotransform. 2008, 26:235-242.
15. Martensson K. Preparation of an immobilized two-enzyme system, β-amylase-pullulanase, to an acrylic copolymer for the conversion of starch to maltose. I. Preparation and stability of immobilized β-amylase. Biotechnol. Bioeng. 1974, 16:567-577.
16. Martensson K. Preparation of an immobilized two-enzyme system, β-amylase-pullulanase, to an acrylic copolymer for the conversion of starch to maltose. II. Co-coupling of the enzymes and use in a packed bed column. Biotechnol. Bioeng. 1974, 16:579-591.
17. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 1959, 31:725-729.
18. Montoro-García S., Gil-Ortiz F., Navarro-Fernández J., Rubio V., García-Carmona F., Sánchez-Ferrer A. Improved cross-linked enzyme aggregates for the production of deascetyl beta-lactam antibiotics intermediates. Bioresour. Technol. 2010, 101:331-336.
19. Namdeo M., Bajpai S.K. Immobilization of α-amylase onto cellulose-coated magnetite (CCM) nanoparticles and preliminary starch degradation study. J. Mol. Catal. B Enzym. 2009, 59:134-139.
20. Nicolov Z.L., Reilly P.J. Enzymatic depolymerisation of starch. Biocatalysts for Industry 1991, 37-62. Plenum Press, New York. J.S. Dordick (Ed.).
21. Ogawa K., Nakajima-Kambe T., Nakahara T., Ko-kufuta E. Co-immobilization of gluconolactonase with glucose oxidase for improvement in kinetic property of enzymatically induced volume collapse in ionic gels. Biomacromolecules 2002, 3:625-631.
22. Park D., Haam S., Jang K., Ahn I.S., Kim W.S. Immobilization of starch-converting enzymes on surface-modified carriers using single and co-immobilized systems: properties and application to starch hydrolysis. Process Biochem. 2005, 40:53-61.
23. Sangeetha K., Abraham T.E. Preparation and characterization of cross-linked enzyme aggregates (CLEA) of subtilisin for controlled release applications. Int. J. Biol. Macromol. 2008, 43:314-319.
24. Schoevaart R., Wolbers M.W., Golubovic M., Ottens M.A., Kieboom P.G., Van Rantwijk F., Van der Wielen L.A.M., Sheldon R.A. Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs). Biotechnol. Bioeng. 2004, 87:754-762.
25. Taboada-Puig R., Junghanns C., Demarche P., Moreira M.T., Feijoo G., Lema J.M., Agathos S.N. Combined cross-linked enzyme aggregates from versatile peroxidase and glucose oxidase: production, partial characterization and application for the elimination of endocrine disruptors. Bioresour. Technol. 2011, 102:6593-6599.
26. Tanriseven A., Uludaǧ Y.B., Doǧan S. A novel method for the immobilization of glucoamylase to produce glucose from maltodextrin. Enzyme Microb. Technol. 2002, 30:406-409.
27. Talekar S., Shah V., Patil S., Nimbalkar M. Porous cross-linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase. Catal. Sci. Technol. 2012, 2:1575-1579.
28. Talekar S., Ghodake V., Ghotage T., Rathod P., Deshmukh P., Nadar S., Mulla M., Ladole M. Novel magnetic cross-linked enzyme aggregates (magnetic CLEAs) of alpha amylase. Bioresour. Technol. 2012, 123:542-547.
29. Talekar S., Desai S., Pillai M., Nagavekar N., Ambarkar S., Surnis S., Ladole M., Nadar S., Mulla M. Carrier free co-immobilization of glucoamylase and pullulanase as combi-cross linked enzyme aggregates (combi-CLEAs). RSC Adv. 2013, 3:2265-2271.
30. Talekar S., Joshi A., Joshi G., Kamat P., Haripurkar R., Kambale S. Parameters in preparation and characterization of cross-linked enzyme aggregates (CLEAs). RSC Adv. 2013, 3:12485-12511.
31. Wang M., Jia C., Qi W., Yu Q., Peng X., Su R., He Z. Porous-CLEAs of papain: application to enzymatic hydrolysis of macromolecules. Bioresour. Technol. 2011, 102:3541-3545.
32. Xue R., Woodley J.M. Process technology for multi-enzymatic reaction systems. Bioresour. Technol. 2012, 115:183-195.
33. Zhang L., Zhu X., Zheng S., Sun H. Photochemical preparation of magnetic chitosan beads for immobilization of pullulanase. Biochem. Eng. J. 2009, 46:83-87.
34. Zhao G., Wang J., Li Y., Huang H., Chen X. Reversible immobilization of glucoamylase onto metal-ligand functionalized magnetic FeSBA-15. Biochem. Eng. J. 2012, 68:159-166.
35. Zhen Q., Wang M., Qi W., Su R., He Z. Preparation of β-mannanase CLEAs with macromolecular cross-linker. Catal. Sci. Technol. 2013, 3:1937-1941.