Real-time imaging of αv integrin molecular dynamics in osteosarcoma cells in vitro and in vivo

Anticancer Research

Volumn 33, Issue 8, 2013, Pages 3021-3026

  Tome, Yasunori ^a,b,c   Sugimoto, Naotoshi ^d   Yano, Shuya ^a,b   Momiyama, Masashi ^a,b   Mii, Sumiyuki ^a,b   Maehara, Hiroki ^c   Bouvet, Michael ^a,b   Tsuchiya, Hiroyuki ^d   Kanaya, Fuminori ^c   Hoffman, Robert M ^a,b  

^a ANTICANCER INC   (United States)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^c UNIVERSITY OF THE RYUKYUS   (Japan)

^d KANAZAWA UNIVERSITY   (Japan)

Author keywords

v Integrin; Confocal microscopy; Fibronectin; Green fluorescent protein; In vitro; In vivo; Metastasis; Nude mouse; Osteosarcoma; Real time imaging

Indexed keywords

CD51 ANTIGEN; COLLAGEN FIBER; FIBRONECTIN; GREEN FLUORESCENT PROTEIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR DYNAMICS; MOLECULAR IMAGING; MOUSE; NONHUMAN; OSTEOSARCOMA CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ΑV INTEGRIN; CONFOCAL MICROSCOPY; FIBRONECTIN; GREEN FLUORESCENT PROTEIN; IN VITRO; IN VIVO; METASTASIS; NUDE MOUSE; OSTEOSARCOMA; REAL-TIME IMAGING;

ANIMALS; CELL LINE, TUMOR; COMPUTER SYSTEMS; DIAGNOSTIC IMAGING; FIBRONECTINS; GREEN FLUORESCENT PROTEINS; HUMANS; INTEGRIN ALPHAV; MICE; MICE, NUDE; MOLECULAR DYNAMICS SIMULATION; OSTEOSARCOMA; PROTEIN BINDING; TIBIA;

EID: 84883277908     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Yao ES, Zhang H, Chen YY, Lee B, Chew K, Moore D and Park C: Increased β1 integrin is associated with decreased survival in invasive breast cancer. Cancer Res 67: 659-664, 2007.
2. Okamura M, Yamaji S, Nagashima Y, Nishikawa M, Yoshimoto N, Kido Y, Iemoto Y, Aoki I and Ishigatsubo Y: Prognostic value of integrin β1-ILK-pAKT signaling pathway in non-small cell lung cancer. Hum Pathol 38: 1081-1091, 2007.
3. Nikkola J, Vihinen P, Vlaykova T, Hahka-Kemppinen M, Heino J and Pyrhonen S: Integrin chains β1 and αv as prognostic factors in human metastatic melanoma. Melanoma Res 14: 29-37, 2004.
4. Grzesiak JJ, Tran Cao HS, Burton DW, Kaushal S, Vargas F, Clopton P, Snyder CS, Deftos LJ, Hoffman RM and Bouvet M: Knockdown of the β1 integrin subunit reduces primary tumor growth and inhibits pancreatic cancer metastasis. Int J Cancer 129: 2905-2915, 2011.
5. Kimura H, Tome Y, Momiyama M, Hayashi K, Tsuchiya H, Bouvet M and Hoffman RM: Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice. Int J Cancer 131: 2027-2033, 2012.
6. Uehara F, Tome Y, Yano S, Miwa S, Mii S, Hiroshima Y, Bouvet M, Maehara H, Kanaya F and Hoffman RM: A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized in vivo. Anticancer Res 33: 1361-1366, 2013.
7. Tome Y, Tsuchiya H, Hayashi K, Yamauchi K, Sugimoto N, Kanaya F, Tomita K and Hoffman RM: In vivo gene transfer between interacting human osteosarcoma cell lines is associated with acquisition of enhanced metastatic potential. J Cell Biochem 108: 362-367, 2009.
8. Miwa S, Tome Y, Yano S, Hiroshima Y, Uehara F, Mii S, Kimura H, Hayashi K, Tsuchiya H, Bouvet M, Efimova EV and Hoffman RM: Single cell time-lapse imaging of focus formation by the DNA damage-response protein 53BP1 after UVC irradiation of human pancreatic cancer cells. Anticancer Res 33: 1373-1377, 2013.
9. Marshall JF and Hart IR: The role of αv-integrins in tumour progression and metastasis. Semin Cancer Biol 7: 129-138, 1996.
10. Kissa K, Murayama E, Zapata A, Cortes A, Perret E, Machu C and Herbomel P: Live imaging emerging hematopoietic stem cells and early thymus colonization. Blood 111: 1147-1156, 2008.
11. Kubow KE and Horwits AR: Reducing background fluorescence reveals adhesions in 3D matrices. Nat Cell Biol 13: 3-5, 2011.
12. Uchugonova A, Zhao M, Weinigel M, Zhang Y, Bouvet M, Hoffman RM and Koenig K: Multiphoton tomography visualizes collagen fibers in the tumor microenvironment that maintain cancercell anchorage and shape. J. Cell. Biochem 114: 99-102, 2013.
13. Hoffman RM. The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer 5: 796-806, 2005.
14. Hoffman RM and Yang M. Subcellular imaging in the live mouse. Nature Protocols 1: 775-782, 2006.
15. Hoffman RM and Yang M. Color-coded fluorescence imaging of tumor-host interactions. Nature Protocols 1: 928-935, 2006.
16. Hoffman RM and Yang M. Whole-body imaging with fluorescent proteins. Nature Protocols 1: 1429-1438, 2006.
17. Yang M, Baranov E, Wang JW, Jiang P, Wang X, Sun FX, Bouvet M, Moossa AR, Penman S and Hoffman RM. Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model Proc. Natl. Acad. Sci. USA 99: 3824-3829, 2002.
18. Yang M, Baranov E, Jiang P, Sun FX, Li XM. Li L, Hasegawa S, Bouvet M, Ai-Tuwaijri M, Chishima T, Shimada H, Moossa AR, Penman S and Hoffman RM. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases, Proc. Natl. Acad. Sci. USA 97: 1206-1211, 2000.