Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis

Methods in Molecular Biology

Volumn 1017, Issue , 2013, Pages 193-199

  Holmberg, Mats ^a   Nollen, Ellen A A ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

Aggregation intermediates; C. elegans; Native agarose gel electrophoresis; Polyglutamine; Protein aggregation

Indexed keywords

POLYGLUTAMINE;

AGAR GEL ELECTROPHORESIS; ARTICLE; CAENORHABDITIS ELEGANS; GENETIC SCREENING; LYSIS; MICROSCOPY; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; NONHUMAN; PARKINSON DISEASE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN MODIFICATION;

CAENORHABDITIS ELEGANS;

EID: 84883180256     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-438-8_14     Document Type: Article

References (21)

1. Chiti F, Dobson CM (2006) Protein misfolding, functional amyloid, and human disease. Annu Rev Biochem 75:333-366
2. Nollen EA, Garcia SM, van Haaften G, Kim S, Chavez A, Morimoto RI, Plasterk RH (2004) Genome-wide RNA interference screen identifies previously undescribed regulators of polyglutamine aggregation. Proc Natl Acad Sci USA 101:6403-6408
3. van Ham TJ, Thijssen KL, Breitling R, Hofstra RM, Plasterk RH, Nollen EA (2008) C. elegans model identifies genetic modifiers of alpha-synuclein inclusion formation during aging. PLoS Genet 4:e1000027
4. Wang J, Farr GW, Hall DH, Li F, Furtak K, Dreier L, Horwich AL (2009) An ALS-linked mutant sod1 produces a locomotor defect associated with aggregation and synaptic dysfunction when expressed in neurons of Caenorhabditis elegans. PLoS Genet 5:e1000350
5. Silva MC, Fox S, Beam M, Thakkar H, Amaral MD, Morimoto RI (2011) A genetic screening strategy identifies novel regulators of the proteostasis network. PLoS Genet 7:e1002438
6. van Ham TJ, Holmberg MA, van der Goot AT, Teuling E, Garcia-Arencibia M, Kim HE, Du D, Thijssen KL, Wiersma M, Burggraaff R, van Bergeijk P, van Rheenen J, Jerre van Veluw G, Hofstra RM, Rubinsztein DC, Nollen EA (2010) Identification of MOAG-4/SERF as a regulator of age-related proteotoxicity. Cell 142:601-612
7. Kamath RS, Ahringer J (2003) Genome-wide RNAi screening in Caenorhabditis elegans. Methods 30:313-321
8. Pothof J, van Haaften G, Thijssen K, Kamath RS, Fraser AG, Ahringer J, Plasterk RHA, Tijsterman M (2003) Identification of genes that protect the C. elegans genome against mutations by genomewide RNAi. Genes Dev 17:443-448
9. van Haaften G, Vastenhouw NL, Nollen EA, Plasterk RH, Tijsterman M (2004) Gene interactions in the DNA damage-response pathway identified by genome-wide RNA-interference analysis of synthetic lethality. Proc Natl Acad Sci USA 101:12992-12996
10. Jorgensen EM, Mango SE (2002) The art and design of genetic screens: Caenorhabditis elegans. Nat Rev Genet 3:356-369
11. Gidalevitz T, Krupinski T, Garcia S, Morimoto RI (2009) Destabilizing protein polymorphisms in the genetic background direct phenotypic expression of mutant SOD1 toxicity. PLoS Genet 5:e1000399
12. Wanker EE, Scherzinger E, Heiser V, Sittler A, Eickhoff H, Lehrach H (1999) Membranefilter assay for detection of amyloid-like polyglutamine- containing protein aggregates. Methods Enzymol 309:375-386
13. Bagriantsev SN, Kushnirov VV, Liebman SW (2006) Analysis of amyloid aggregates using agarose gel electrophoresis. Methods Enzymol 412:33-48
14. Halfmann R, Lindquist S (2008) Screening for amyloid aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis. J Vis Exp (17). pii: 838. doi: 10.3791/838
15. Weiss A, Klein C, Woodman B, Sathasivam K, Bibel M, Regulier E, Bates GP, Paganetti P (2008) Sensitive biochemical aggregate detection reveals aggregation onset before symptom development in cellular and murine models of Huntington's disease. J Neurochem 104:846-858
16. Yerkes S, Vesenka J, Kmiec EB (2010) A stable G-quartet binds to a huntingtin protein fragment containing expanded polyglutamine tracks. J Neurosci Res 88:335-345
17. Kryndushkin DS, Alexandrov IM, Ter-Avanesyan MD, Kushnirov VV (2003) Yeast [PSI+] prion aggregates are formed by small Sup35 polymers fragmented by Hsp104. J Biol Chem 278:49636-49643
18. Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671-675
19. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77:71-94
20. Wittig I, Braun HP, Schagger H (2006) Blue native PAGE. Nat Protoc 1:418-428
21. Kim R, Yokota H, Kim SH (2000) Electrophoresis of proteins and protein-protein complexes in a native agarose gel. Anal Biochem 282:147-149