Screening for amyloid aggregation by semi-denaturing detergent-agarose gel electrophoresis

Journal of Visualized Experiments

Volumn , Issue 17, 2008, Pages

  Halfmann, Randal ^a,b,c   Lindquist, Susan ^a,b,c  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055112289     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/838     Document Type: Article

References (10)

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2. Alexandrov, I.M., Vishnevskaya, A.B., Ter-Avanesyan, M.D. & Kushnirov, V.V. Appearance and Propagation of Polyglutamine-based Amyloids in Yeast: TYROSINE RESIDUES ENABLE POLYMER FRAGMENTATION. J. Biol. Chem. 283, 15185-15192 (2008).
3. Allen, K.D. et al. Hsp70 chaperones as modulators of prion life cycle: novel effects of Ssa and Ssb on the Saccharomyces cerevisiae prion [PSI+]. Genetics 169, 1227-1242 (2005).
4. Aron, R., Higurashi, T., Sahi, C. & Craig, E.A. J-protein co-chaperone Sis1 required for generation of [RNQ+] seeds necessary for prion propagation. The EMBO journal 26, 3794-3803 (2007).
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7. Salnikova, A.B., Kryndushkin, D.S., Smirnov, V.N., Kushnirov, V.V. & Ter-Avanesyan, M.D. Nonsense suppression in yeast cells overproducing Sup35 (eRF3) is caused by its non-heritable amyloids. J. Biol. Chem. 280, 8808-8812 (2005).
8. Tank, E.M., Harris, D.A., Desai, A.A. & True, H.L. Prion protein repeat expansion results in increased aggregation and reveals phenotypic variability. Mol. Cell. Biol. 27, 5445-5455 (2007).
9. Douglas, P.M. et al. Chaperone-dependent amyloid assembly protects cells from prion toxicity. Proc. Natl. Acad. Sci. USA 105, 7206-7211 (2008).
10. Nagy, B., Costello, R., and Csako, G. Downward blotting of proteins in a model based on apolipoprotein(a) phenotyping. Analytical Biochemistry. 231, 40-45 (1995).