Simultaneous detection by multiplex PCR of the high-temperature-growing Pythium species: P. aphanidermatum, P. helicoides and P. myriotylum

Journal of General Plant Pathology

Volumn 79, Issue 5, 2013, Pages 350-358

  Ishiguro, Yasushi ^a   Asano, Takahiro ^a   Otsubo, Kayoko ^a   Suga, Haruhisa ^a   Kageyama, Koji ^a  

^a GIFU UNIVERSITY   (Japan)

Author keywords

Disease management; Molecular detection; Molecular diagnosis; Multiplex PCR

Indexed keywords

EUKARYOTA; PYTHIUM; PYTHIUM APHANIDERMATUM; PYTHIUM HELICOIDES; PYTHIUM MYRIOTYLUM;

EID: 84883049326     PISSN: 13452630     EISSN: 1610739X     Source Type: Journal    
DOI: 10.1007/s10327-013-0466-2     Document Type: Article

References (33)

1. Ali-Shtayeh MS, Lim-Ho Chee Len, Dick MW (1986) An improved method and medium for quantitative estimates of populations of Pythium species from soil. Trans Br Mycol Soc 86: 39-47.
2. Asano T, Kageyama K, Hyakumachi M (1999) Surface disinfestation of resting spores of Plasmodiophora brassicae used to infect hairy roots of Brassica spp. Phytopathology 89: 314-319.
3. Asano T, Senda M, Suga H, Kageyama K (2010) Development of multiplex PCR to detect five Pythium species related to turfgrass diseases. J Phytopathol 158: 609-615.
4. Blair JE, Coffey MD, Park SY, Geiser DM, Kang S (2008) A multi-locus phylogeny for Phytophthora utilizing markers derived from complete genome sequences. Fungal Genet Biol 45: 266-277.
5. Godfrey SAC, Monds RD, Lash DT, Marshall JW (2003) Identification of Pythium oligandrum using species-specific ITS rDNA PCR oligonucleotides. Mycol Res 107: 790-796.
6. Ishii K, Fukui M (2001) Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR. Appl Environ Microbiol 67: 3753-3755.
7. Kageyama K (2011) Characteristics of high-temperature-growing Pythium species nowadays frequently occurring disease (in Japanese). Plant Protect 65: 102-106.
8. Kageyama K, Ui T (1982) Survival structure of Pythium spp. in the soils of bean fields. Ann Phytopath Soc Japan 48: 308-313.
9. Kageyama K, Ohyama A, Hyakumachi M (1997) Detection of Pythium ultimum using polymerase chain reaction with species specific primers. Plant Dis 81: 1155-1160.
10. Kageyama K, Aoyagi T, Sunouchi R, Fukui H (2002) Root rot of miniature roses caused by Pythium helicoides. J Gen Plant Pathol 68: 15-20.
11. Kageyama K, Komatsu T, Suga H (2003) Refined PCR protocol for detection of plant pathogens in soil. J Gen Plant Pathol 69: 153-160.
12. Kageyama K, Senda M, Asano T, Suga H, Iishiguro K (2007) Intra-isolate heterogeneity of the ITS region of rDNA in Pythium helicoides. Mycol Res 111: 416-423.
13. Kernaghan G, Reeleder RD, Hoke SMT (2008) Quantification of Pythium populations in ginseng soils by culture dependent and real time PCR methods. Appl Soil Ecol 40: 447-455.
14. Lèvesque CA, de Cock AWAM (2004) Molecular phylogeny and taxonomy of the genus Pythium. Mycol Res 108: 1363-1383.
15. Li M, Senda M, Komatsu T, Suga H, Kageyama K (2010) Development of real-time PCR technique for the estimation of population density of Pythium intermedium in forest soils. Microbiol Res 165: 695-705.
16. Li M, Asano T, Suga H, Kageyama K (2011) A multiplex PCR for the detection of Phytophthora nicotianae and P. cactorum, and a survey of their occurrence in strawberry production areas of Japan. Plant Dis 95: 1270-1278.
17. Meherun N, Motohash K, Watanabe H, Chikuo Y, Senda M, Suga H, Brasier C, Kageyama K (2011) Phytophthora chrysanthemi sp. nov., a new species causing root rot of chrysanthemum in Japan. Mycol Progress 10: 21-31.
18. Miyake N, Nagai H, Fukuta S, Kuroyanagi S, Takahashi R, Kageyama K (2012) Occurrence of Pythium root rot of poinsettia caused by Pythium myriotylum Drechsler and Pythium helicoides Drechsler (abstract in Japanese). Jpn J Phytopathol 78: 183.
19. Morita Y, Tojo M (2007) Modifications of PARP medium using fluazinam, miconazole, and nystatin for detection of Pythium spp. in soil. Plant Dis 91: 1591-1599.
20. Schroeder KL, Okubara PA, Tambong JT, Lèvesque CA, Paulitz TC (2006) Identification and quantification of pathogenic Pythium spp. from soils in eastern Washington using real-time polymerase chain reaction. Phytopathology 96: 637-647.
21. Schroeder KL, Martin FN, de Cock AWAM, Lèvesque CA, Spies CFJ, Okubara PA, Paulitz TC (2013) Molecular detection and quantification of Pythium species: evolving taxonomy, new tools, and challenges. Plant Dis 97: 4-20.
22. Suzuki M, Togawa M, Yoneyama C (2005) Occurrence of Pythium root rot of strawberry caused by Pythium helicoides (abstract in Japanese). Jpn J Phytopathol 71: 209.
23. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876-4882.
24. van der Plaats-Niterink AJ (1981) Monograph of the genus Pythium. Study Mycol 21: 1-242.
25. Wang PH, Chang CW (2003) Detection of the low-germination-rate resting oospores of Pythium myriotylum from soil by PCR. Lett Appl Microbiol 36: 157-161.
26. Wang PH, Boo LM, Lin YS, Yeh Y (2002) Specific detection of Pythium aphanidermatum from hydroponic nutrient solution by booster PCR with DNA primers developed from mitochondrial DNA. Phytoparasitica 30: 473-485.
27. Wang PH, Chung CY, Lin YS, Yeh Y (2003a) Use of polymerase chain reaction to detect the soft rot pathogen, Pythium myriotylum, in infected ginger rhizomes. Lett Appl Microbiol 36: 116-120.
28. Wang PH, Wang YT, White JG (2003b) Species-specific PCR primers for Pythium developed from ribosomal ITS1 region. Lett Appl Microbiol 37: 127-132.
29. Watanabe T (1977) Pathogenicity of Pythium myriotylum isolated from strawberry roots in Japan. Ann Phytopath Soc Japan 43: 306-309.
30. Watanabe H, Horinouchi H, Tanahashi I, Kageyama K (2005) Occurrence of root rot of strawberry caused by Pythiumhelicoides, and pathogenicity to several crops (abstract in Japanese). Jpn J Phytopathol 71: 209-210.
31. Watanabe H, Taguchi Y, Horinouchi H, Hyakumachi M, Kageyama K (2007) Pythium and Phytophthora species associated with root and stem rots of kalanchoe. J Gen Plant Pathol 73: 81-88.
32. Watanabe H, Kageyama K, Taguchi Y, Horinouchi H, Hyakumachi M (2008) Bait method to detect Pythium species that grow at high temperatures in hydroponic solutions. J Gen Plant Pathol 74: 417-424.
33. Yin-Ling, Kageyama K, Asano T, Senda M, Watanabe H, Suga H, Fukui H (2007) Detection of Pythium helicoides in flower production area using PCR with species-specific primers. Jpn J Phytopathol 73: 86-93.