-
1
-
-
0842278671
-
Laboratory evolution of cytochrome p450 bm-3 monooxygenase for organic cosolvents
-
Wong TS, Arnold FH, Schwaneberg U (2004) Laboratory evolution of cytochrome P450 BM-3 monooxygenase for organic cosolvents. Biotechnol Bioeng 85:351-358
-
(2004)
Biotechnol Bioeng
, vol.85
, pp. 351-358
-
-
Wong, T.S.1
Arnold, F.H.2
Schwaneberg, U.3
-
2
-
-
40749104246
-
Cytochrome p450 bm-3 evolved byrandom and saturation mutagenesis as an effective indole-hydroxylating catalyst
-
Li HM, Mei LH, Urlacher VB, Schmid RD (2008) Cytochrome P450 BM-3 evolved by random and saturation mutagenesis as an effective indole-hydroxylating catalyst. Appl Biochem Biotechnol 144:27-36
-
(2008)
Appl Biochem Biotechnol
, vol.144
, pp. 27-36
-
-
Li, H.M.1
Mei, L.H.2
Urlacher, V.B.3
Schmid, R.D.4
-
3
-
-
0034667190
-
High ef ficiency family shuffling based on multi-step pcr and in vivo dna recombination in yeast: Statistical and functional analysis of a combinatorial library between human cytochrome p450 1a1 and 1a2
-
Abecassis V, Pompon D, Truan G (2000) High ef ficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2. Nucleic Acids Res 28:E88
-
(2000)
Nucleic Acids Res
, vol.28
-
-
Abecassis, V.1
Pompon, D.2
Truan, G.3
-
4
-
-
0036289574
-
Protein building blocks preserved by recombination
-
Voigt CA, Martinez C, Wang ZG, Mayo SL, Arnold FH (2002) Protein building blocks preserved by recombination. Nat Struct Biol 9:553-558
-
(2002)
Nat Struct Biol
, vol.9
, pp. 553-558
-
-
Voigt, C.A.1
Martinez, C.2
Wang, Z.G.3
Mayo, S.L.4
Arnold, F.H.5
-
5
-
-
0032518266
-
Dna shuffling of a family of genes from diverse species accelerates directed evolution
-
Crameri A, Raillard SA, Bermudez E, Stemmer WP (1998) DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature 391:288-291
-
(1998)
Nature
, vol.391
, pp. 288-291
-
-
Crameri, A.1
Raillard, S.A.2
Bermudez, E.3
Stemmer, W.P.4
-
6
-
-
0028110130
-
Dna shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
-
Stemmer WP (1994) DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Proc Natl Acad Sci U S A 91:10747-10751
-
(1994)
Proc Natl Acad Sci U S A
, vol.91
, pp. 10747-10751
-
-
Stemmer, W.P.1
-
7
-
-
35748939419
-
A shuffled cyp2c library with a high degree of structural integrity and functional versatility
-
Huang W, Johnston WA, Hayes MA, De Voss JJ, Gillam EM (2007) A shuffled CYP2C library with a high degree of structural integrity and functional versatility. Arch Biochem Biophys 467:193-205
-
(2007)
Arch Biochem Biophys
, vol.467
, pp. 193-205
-
-
Huang, W.1
Johnston, W.A.2
Hayes, M.A.3
De Voss, J.J.4
Gillam, E.M.5
-
8
-
-
36348968330
-
A shuffled cyp1a library shows both structural integrity and functional diversity
-
Johnston WA, Huang W, De Voss JJ, Hayes MA, Gillam EM (2007) A shuffled CYP1A library shows both structural integrity and functional diversity. Drug Metab Dispos 35: 2177-2185
-
(2007)
Drug Metab Dispos
, vol.35
, pp. 2177-2185
-
-
Johnston, W.A.1
Huang, W.2
De Voss, J.J.3
Hayes, M.A.4
Gillam, E.M.5
-
9
-
-
80555150588
-
Facile production of minor metabolites for drug development using a cyp3a shuffled library
-
Hunter DJ, Behrendorff JB, Johnston WA, Hayes PY, Huang W, Bonn B, Hayes MA, De Voss JJ, Gillam EM (2011) Facile production of minor metabolites for drug development using a CYP3A shuffled library. Metab Eng 13:682-693
-
(2011)
Metab Eng
, vol.13
, pp. 682-693
-
-
Hunter, D.J.1
Behrendorff, J.B.2
Johnston, W.A.3
Hayes, P.Y.4
Huang, W.5
Bonn, B.6
Hayes, M.A.7
De Voss, J.J.8
Gillam, E.M.9
-
11
-
-
84866379763
-
Directed evolution reveals requisite sequence elements in the functional expression of p450 2f1 in escherichia coli
-
Behrendorff JBYH, Moore CD, Kim K-H, Kim D-H, Smith CA, Johnston WA, Yun C-H, Yost GS, Gillam EMJ (2012) Directed evolution reveals requisite sequence elements in the functional expression of P450 2F1 in Escherichia coli. Chem Res Toxicol 25: 1964-1974.
-
(2012)
Chem Res Toxicol
, vol.25
, pp. 1964-1974
-
-
Jbyh, B.1
Moore, C.D.2
Kim, K.-H.3
Kim, D.-H.4
Smith, C.A.5
Johnston, W.A.6
Yun, C.-H.7
Yost, G.S.8
Gillam, E.M.J.9
-
12
-
-
0028050350
-
Rapid evolution of a protein in vitro by dna shuffling
-
Stemmer WP (1994) Rapid evolution of a protein in vitro by DNA shuffling. Nature 370:389-391
-
(1994)
Nature
, vol.370
, pp. 389-391
-
-
Stemmer, W.P.1
-
13
-
-
34247626257
-
Extending the diversity of cytochrome p450 enzymes by dna family shuffling
-
Rosic NN, Huang W, Johnston WA, DeVoss JJ, Gillam EM (2007) Extending the diversity of cytochrome P450 enzymes by DNA family shuffling. Gene 395:40-48
-
(2007)
Gene
, vol.395
, pp. 40-48
-
-
Rosic, N.N.1
Huang, W.2
Johnston, W.A.3
DeVoss, J.J.4
Gillam, E.M.5
-
14
-
-
0032814117
-
Novel family shuffling methods for the in vitro evolution of enzymes
-
Kikuchi M, Ohnishi K, Harayama S (1999) Novel family shuffling methods for the in vitro evolution of enzymes. Gene 236: 159-167
-
(1999)
Gene
, vol.236
, pp. 159-167
-
-
Kikuchi, M.1
Ohnishi, K.2
Harayama, S.3
-
15
-
-
0025994649
-
Expression and enzymatic activity of recombinant cytochrome p450 17 alpha-hydroxylase in escherichia coli
-
Barnes HJ, Arlotto MP, Waterman MR (1991) Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli . Proc Natl Acad Sci U S A 88: 5597-5601
-
(1991)
Proc Natl Acad Sci U S A
, vol.88
, pp. 5597-5601
-
-
Barnes, H.J.1
Arlotto, M.P.2
Waterman, M.R.3
-
16
-
-
39049159646
-
Quantitative wholecell cytochrome p450 measurement suitable for high-throughput application
-
Johnston WA, Huang W, De Voss JJ, Hayes MA, Gillam EM (2008) Quantitative wholecell cytochrome P450 measurement suitable for high-throughput application. J Biomol Screen 13:135-141
-
(2008)
J Biomol Screen
, vol.13
, pp. 135-141
-
-
Johnston, W.A.1
Huang, W.2
De Voss, J.J.3
Hayes, M.A.4
Gillam, E.M.5
-
17
-
-
84892903674
-
Using plasmid vectors in molecular cloning
-
In: Samuel D, Russell DW (eds) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
-
Sambrook J, Russell DW (2006) Using plasmid vectors in molecular cloning. In: Samuel D, Russell DW (eds) The condensed protocols from molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 2-8
-
(2006)
The Condensed Protocols from Molecular Cloning: A Laboratory Manual
, pp. 2-8
-
-
Sambrook, J.1
Russell, D.W.2
-
18
-
-
0028930681
-
Reverse electrophoresis to concentrate dna fractions
-
Kadokami Y, Lewis RV (1995) Reverse electrophoresis to concentrate DNA fractions. Anal Biochem 226:193-195
-
(1995)
Anal Biochem
, vol.226
, pp. 193-195
-
-
Kadokami, Y.1
Lewis, R.V.2
-
19
-
-
0030843652
-
Drug metabolism by escherichia coli expressing human cytochromes p450
-
Parikh A, Gillam EM, Guengerich FP (1997) Drug metabolism by Escherichia coli expressing human cytochromes P450. Nat Biotechnol 15:784-788
-
(1997)
Nat Biotechnol
, vol.15
, pp. 784-788
-
-
Parikh, A.1
Gillam, E.M.2
Guengerich, F.P.3
-
20
-
-
0025675856
-
High ef ficiency transformation of escherichia coli with plasmids
-
Inoue H, Nojima H, Okayama H (1990) High ef ficiency transformation of Escherichia coli with plasmids. Gene 96:23-28
-
(1990)
Gene
, vol.96
, pp. 23-28
-
-
Inoue, H.1
Nojima, H.2
Okayama, H.3
|