Transcriptomic and phospho-proteomic analyzes of erythroblasts expanded in vitro from normal donors and from patients with polycythemia vera

American Journal of Hematology

Volumn 88, Issue 9, 2013, Pages 723-729

  Hricik, Todd ^a   Federici, Giulia ^b,c,d   Zeuner, Ann ^c   Alimena, Giuliana ^c,e   Tafuri, Agostino ^e   Tirelli, Valentina ^c   Varricchio, Lilian ^f   Masiello, Francesca ^c   Ciaffoni, Fiorella ^c   Vaglio, Stefania ^c   Petricoin, Emanuel F ^d   Girelli, Gabriella ^c   Levine, Ross L ^a   Migliaccio, Anna Rita F ^c,f  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b REGINA ELENA NATIONAL CANCER INSTITUTE   (Italy)

^c ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^d GEORGE MASON UNIVERSITY   (United States)

^e SAPIENZA UNIVERSITY OF ROME   (Italy)

^f MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEXAMETHASONE; ERYTHROPOIETIN; INTERLEUKIN 3; JANUS KINASE 2; LYMPHOTOXIN; PHOSPHATIDYLINOSITOL 3 KINASE; RNA; STAT PROTEIN; STEM CELL FACTOR; TRANSCRIPTION FACTOR GATA 1; TRANSCRIPTION FACTOR TAL1;

ADULT; AGED; ARTICLE; BLOOD DONOR; CELL MATURATION; CLINICAL ARTICLE; CONTROLLED STUDY; ENRICHMENT CULTURE; ERYTHROBLAST; ERYTHROID CELL; ESCAPE BEHAVIOR; FEMALE; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; INTRACELLULAR SIGNALING; MALE; NORMAL HUMAN; PHOSPHORYLATION; POLYCYTHEMIA VERA; PRIORITY JOURNAL; PROTEOMICS; SOMATIC MUTATION; STEM CELL EXPANSION; TRANSCRIPTOMICS;

ADULT; AGED; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; CASE-CONTROL STUDIES; CELLS, CULTURED; DEXAMETHASONE; ERYTHROBLASTS; ERYTHROPOIESIS; ERYTHROPOIETIN; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMANS; INTERLEUKIN-3; MALE; MIDDLE AGED; PHOSPHOPROTEINS; POLYCYTHEMIA VERA; PROTEOMICS; PROTO-ONCOGENE PROTEINS; RAB4 GTP-BINDING PROTEINS; RECEPTORS, G-PROTEIN-COUPLED; SIGNAL TRANSDUCTION; STEM CELL FACTOR;

EID: 84882582645     PISSN: 03618609     EISSN: 10968652     Source Type: Journal    
DOI: 10.1002/ajh.23487     Document Type: Article

References (45)

1. Gilbert HS. Definition, clinical features and diagnosis of polycythaemia vera. (0308-2261), in press.
2. Levine RL, Gillliland, D. Gary, Myeloproliferative disorders. Blood 2008;112(6):2190-2198.
3. Schuringa JJ, et al. Constitutive activation of STAT5A promotes human hematopoietic stem cell self-renewal and erythroid differentiation. J Exp Med 2004;200(5):623-635.
4. Oda A., et al. Erythropoietin induces tyrosine phosphorylation of Jak2, STAT5A, and STAT5B in primary cultured human erythroid precursors. Blood 1998;92(2):443-451.
5. von Lindern M, et al. The glucocorticoid receptor cooperates with the erythropoietin receptor and c-kit to enhance and sustain proliferation of erythroid progenitors in vitro. Blood 1999;94(2):550-559.
6. Panzenbock B, et al. growth and differentiation of human stem cell factor/erythropoietin-dependent erythroid progenitor cells in vitro. Blood 1998;92(10):3658-3668.
7. Migliaccio G, et al. Under HEMA conditions, self-replication of human erythroblasts is limited by autophagic death. Blood Cells Mol Dis 2011;47(3):182-197.
8. Ugo V, et al. Multiple signaling pathways are involved in erythropoietin-independent differentiation of erythroid progenitors in polycythemia vera. Exp Hematol 2004;32(2):179-187.
9. Golde DW, et al. Polycythemia vera: Hormonal modulation of erythropoiesis in vitro. Blood 1977;49(3):399-405.
10. Bruchova H, et al. Erythropoiesis in polycythemia vera is hyper-proliferative and has accelerated maturation. Blood Cells Mol Dis 2009;43(1):81-87.
11. Varricchio L, et al. The dominant negative beta isoform of the glucocorticoid receptor is uniquely expressed in erythroid cells expanded from polycythemia vera patients. Blood 2011;118(2):425-436.
12. Zeuner A, et al. Increased death receptor resistance and FLIPshort expression in polycythemia vera erythroid precursor cells. Blood 2006;107(9):3495-3502.
13. Merryweather-Clarke AT, et al. Global gene expression analysis of human erythroid progenitors. Blood 2011;117(13):e96-e108.
14. Welch JJ, et al. Global regulation of erythroid gene expression by transcription factor GATA-1. Blood 2004;104(10):3136-3147.
15. Peller S, et al. Identification of gene networks associated with erythroid differentiation. Blood Cells Mol Dis 2009;43(1):74-80.
16. Xu J, et al. Combinatorial assembly of developmental stage-specific enhancers controls gene expression programs during human erythropoiesis. Dev Cell 2012;23(4):796-811.
17. Komor M, et al. Transcriptional profiling of human hematopoiesis during in vitro lineage-specific differentiation. Stem Cells 2005;23(8):1154-1169.
18. Narla A, et. al., Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 2011;118(8):2296-2304.
19. Migliaccio G, et al. In vitro mass production of human erythroid cells from the blood of normal donors and of thalassemic patients. Blood Cell Mol Dis 2002;28(2):169-180.
20. <4397949E.pdf>.
21. .
22. Westerterp M, et al. Regulation of hematopoietic stem and progenitor cell mobilization by cholesterol efflux pathways. Cell Stem Cell 2012;11(2):195-206.
23. Dorsam RT, Gutkind, J.S. G-protein-coupled receptors and cancer. Nat Rev Cancer 2007;7(2):79-94.
24. Kim JE, et al. Splicing variants of the orphan G-protein-coupled receptor GPR56 regulate the activity of transcription factors associated with tumorigenesis. J Cancer Res Clin Oncol 2010;136(1):47-53.
25. Hellberg C, et al. Activation of protein kinase C alpha is necessary for sorting the PDGF beta-receptor to Rab4a-dependent recycling. Mol Biol Cell 2009;20(12):2856-2863.
26. Mukhopadhyay SS, et al. Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia. J Cell Biol 2006;175(2):225-235.
27. Orkin SH, Zon, LI. Hematopoiesis: An evolving paradigm for stem cell biology. Cell 2008;132(4):631-644.
28. Mouthon M, et al., Expression of tal-1 and GATA-binding proteins furing human hematopoiesis. Blood 1993;81(3):647-655.
29. Diaz-Blanco E, et al. Molecular signature of CD34(+) hematopoietic stem and progenitor cells of patients with CML in chronic phase. Leukemia 2007;21(3):494-504.
30. Li Z., e.a., Erlotinib effectively inhibits JAK2 V617F activiity and polycythemia vera cell growth. J Biol Chem 2007;282(6):3428-3432.
31. Pardanani A, Tefferi, A. Imatinib targets other than bcr/abl and their clinical relevance in myeloid disorders. Blood 2004;104(7):1931-1939.
32. Anzai N. c-kit associated with the transmembrane 4 superfamily proteins constitutes a functionally distinct subunit in human hematopoietic progenitors. Blood 2002;99(12):4413-4421.
33. Boles B, et al. Chk1 haploinsufficiency results in anemia and defective erythropoiesis. PLoS One 2010;5(1):e8581 1-11.
34. Song MS, Salmena L, Pandolfi, PP. The functions and regulation of the PTEN tumour suppressor. Nat Rev Mol Cell Biol 2012;13(5):283-296.
35. Tamguney T, Stokoe, D. New insights into PTEN. J Cell Sci 2007;120(Pt 23):4071-4079.
36. Oshiro N, et al. The proline-rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1. J Biol Chem 2007;282(28):20329-20339.
37. Wang L, Harris TE, Lawrence JC Jr. Regulation of proline-rich Akt substrate of 40 kDa (PRAS40) function by mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation. J Biol Chem 2008;283(23):15619-15627.
38. Wiza C., N.E.B., Ouwens D.M., Role of PRAS40 in Akt and mTOR signaling in health and disease. Am J Physiol Endocrinol Metab 2012;302:E1453-E1460.
39. Herget T, et al. The myristoylated alanine-rich C-kinase substrate (MARCKS) is sequentially phosphorylated by conventional, novel and atypical isotypes of protein kinase C. Eur J Biochem 1995;233:448-457.
40. von Lindern M, et al. Protein kinase C alpha controls erythropoietin receptor signaling. J Biol Chem 2000;275(44):34719-34727.
41. Shaywitz AGME. CREB: A stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu Rev Biochem 1999;68:821-861.
42. Arthur JS, Cohen P, MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells. FEBS Lett 2000;482:44-48.
43. Mayr B, Montminy M, Transcriptional regulation by the phosphorylation-dependent factor CRE Nat. Rev Mol Cell Bio 2001;2:599-608.
44. Blobel GA, et. al., CREB-binding protein coorperates with transcription factor GATA-1 and is required for erythroid differentiation. Proc Natl Acad Sci 1998;95:2061-2066.
45. Bell ES, Park M. Models of crk adaptor proteins in cancer. Genes Canc 2012;3(5-6):341-352.