Effect of saliva stabilisers on detection of porcine reproductive and respiratory syndrome virus in oral fluid by quantitative reverse transcriptase real-time PCR

Veterinary Journal

Volumn 197, Issue 2, 2013, Pages 224-228

  Decorte, Inge ^a   Van der Stede, Yves ^b,c   Nauwynck, Hans ^c   De Regge, Nick ^a   Cay, Ann Brigitte ^a  

^a VETERINARY AND AGROCHEMICAL RESEARCH CENTRE   (Belgium)

^b CVD ERA   (Belgium)

^c GHENT UNIVERSITY   (Belgium)

Author keywords

Oral fluid; Porcine reproductive and respiratory syndrome virus; Quantitative reverse transcriptase real time PCR

Indexed keywords

STABILIZING AGENT; VIRUS RNA;

ARTERIVIRUS; ARTICLE; BODY FLUID; CONTROLLED STUDY; DIAGNOSTIC KIT; DIAGNOSTIC VALUE; GENE AMPLIFICATION; INTERMETHOD COMPARISON; LIMIT OF DETECTION; NONHUMAN; ORAL FLUID; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA STABILITY; ROOM TEMPERATURE; SALIVA ANALYSIS; STORAGE TEMPERATURE; SWINE; VIRUS DETECTION;

ORAL FLUID; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS; QUANTITATIVE REVERSE TRANSCRIPTASE REAL-TIME PCR;

ANIMALS; EXCIPIENTS; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SALIVA; SPECIMEN HANDLING; SWINE;

PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SUS;

EID: 84881663147     PISSN: 10900233     EISSN: 15322971     Source Type: Journal    
DOI: 10.1016/j.tvjl.2013.02.001     Document Type: Article

References (18)

1. Chittick W.A., Stensland W.R., Prickett J.R., Strait E.L., Harmon K., Yoon K.-J., Wang C., Zimmerman J.J. Comparison of RNA extraction and real-time reverse transcription polymerase chain reaction methods for the detection of porcine reproductive and respiratory syndrome virus in porcine oral fluid specimens. Journal of Veterinary Diagnostic Investigation 2011, 23:248-253.
2. Detmer S.E., Patnayak D.P., Jiang Y., Gramer M.R., Goyal S.M. Detection of influenza A virus in porcine oral fluid samples. Journal of Veterinary Diagnostic Investigation 2011, 23:241-247.
3. Eichel H.J., Conger N., Chernick W.S. Acid and alkaline ribonucleases of human parotid, submaxillary, and whole saliva. Archives of Biochemistry and Biophysics 1964, 107:197-208.
4. Gregory R.L., Kim D.E., Kindle J.C., Hobbs L.C., Lloyd D.R. Immunoglobulin-degrading enzymes in localized juvenile periodontitis. Journal of Periodontal Research 1992, 27:176-183.
5. Hedman J., Nordgaard A., Rasmusson B., Ansell R., Rådstrom P. Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modelling of DNA profiles. BioTechniques 2009, 47:951-958.
6. Kittawornrat A., Prickett J., Chittick W., Wang C., Engle M., Johnson J., Patnayak D., Schwartz T., Whitney D., Olsen C., Schwartz K., Zimmerman J. Porcine reproductive and respiratory syndrome virus (PRRSV) in serum and oral fluid samples from individual boars: Will oral fluid replace serum for PRRSV surveillance?. Virus Research 2010, 154:170-176.
7. Ochert A.S., Boulter A.W., Birnbaum W., Johnson N.W., Teo C.G. Inhibitory effect of salivary fluids on PCR: Potency and removal. PCR Methods and Applications 1994, 3:365-368.
8. Oleksiewicz M.B., Bøtner A., Madsen K.G., Storgaard T. Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes. Veterinary Microbiology 1998, 64:7-22.
9. Prickett J.R., Kim W., Simer R., Yoon K.-J., Zimmerman J. Oral-fluid samples for surveillance of commercial growing pigs for porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 infections. Journal of Swine Health and Production 2008, 16:86-91.
10. Prickett J., Simer R., Christopher-Hennings J., Yoon K.-J., Evans R.B., Zimmerman J.J. Detection of porcine reproductive and respiratory syndrome virus infection in porcine oral fluid samples: A longitudinal study under experimental conditions. Journal of Veterinary Diagnostic Investigation 2008, 20:156-163.
11. Prickett J.R., Cutler S., Kinyon J., Naberhaus N., Stensland W.R., Yoon K.-J., Zimmerman J.J. Stability of porcine reproductive and respiratory syndrome virus and antibody in swine oral fluid. Journal of Swine Health and Production 2010, 18:187-195.
12. Prickett J.R., Johnson J., Murtaugh M.P., Puvanendiran S., Wang C., Zimmerman J.J., Opriessnig T. Prolonged detection of PCV2 and anti-PCV2 antibody in oral fluids following experimental inoculation. Transboundary and Emerging Diseases 2010, 58:121-127.
13. Ramirez A., Wang C., Prickett J.R., Pogranichniy R., Yoon K.-J., Main R., Johnson J.K., Rademacher C., Hoogland M., Hoffmann P., Kurtz A., Zimmerman J. Efficient surveillance of pig populations using oral fluids. Preventive Veterinary Medicine 2012, 104:292-300.
14. Romagosa A., Gramer M., Joo H.S., Torremorell M. Sensitivity of oral fluids for detecting influenza A virus in populations of vaccinated and non-vaccinated pigs. Influenza and Other Respiratory Viruses 2012, 6:110-118.
15. Schipper R.G., Siletti E., Vingerhoeds M.H. Saliva as research material: Biochemical, physicochemical and practical aspects. Archives of Oral Biology 2007, 52:1114-1135.
16. Taylor S., Wakem M., Dijkman G., Alsarraj M., Nguyen M. A practical approach to RT-qPCR-publishing data that conform to the MIQE guidelines. Methods 2010, 50:S1-S5.
17. Van Breedam W., Costers S., Vanhee M., Gagnon C.A., Rodríguez-Gómez I.M., Geldhof M., Verbeeck M., Van Doorsselaere J., Karniychuk U., Nauwynck H.J. Porcine reproductive and respiratory syndrome virus (PRRSV)-specific mAbs: Supporting diagnostics and providing new insights into the antigenic properties of the virus. Veterinary Immunology and Immunopathology 2011, 141:246-257.
18. Wasilk A., Callahan J.D., Christopher-Hennings J., Gay T.A., Fang Y., Dammen M., Reos M.E., Torremorell M., Polson D., Mellencamp M., Nelson E., Nelson W.M. Detection of U.S., Lelystad, and European-like porcine reproductive and respiratory syndrome viruses and relative quantitation in boar semen and serum samples by real-time PCR. Journal of Clinical Microbiology 2004, 42:4453-4461.