Sensitivity of oral fluids for detecting influenza A virus in populations of vaccinated and non-vaccinated pigs

Influenza and other Respiratory Viruses

Volumn 6, Issue 2, 2012, Pages 110-118

  Romagosa, Anna ^a   Gramer, Marie ^a   Joo, Han Soo ^a   Torremorell, Montserrat ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Diagnostic; Influenza virus; Oral fluids; Surveillance; Swine

Indexed keywords

INFLUENZA VACCINE; VIRUS RNA;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; FEMALE; FLUID INTAKE; INFLUENZA A; INFLUENZA VACCINATION; INFLUENZA VIRUS A H1N1; NONHUMAN; NOSE SMEAR; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS ISOLATION;

ANIMALS; INFLUENZA A VIRUS; INFLUENZA VACCINES; MOLECULAR DIAGNOSTIC TECHNIQUES; NOSE; ORTHOMYXOVIRIDAE INFECTIONS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SALIVA; SENSITIVITY AND SPECIFICITY; SWINE; SWINE DISEASES; VETERINARY MEDICINE; VIROLOGY;

EID: 84857055644     PISSN: 17502640     EISSN: 17502659     Source Type: Journal    
DOI: 10.1111/j.1750-2659.2011.00276.x     Document Type: Article

References (26)

1. Prickett JR, Kim W, Simer R, Yoon KJ, Zimmerman J. Oral-fluid samples for surveillance of commercial growing pigs for porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 infections. J Swine Health Prod 2008; 16:86-91.
2. Detmer SE, Patnayak DP, Jiang Y, Gramer MR, Goyal SM. Detection of Influenza A virus in porcine oral fluid samples. J Vet Diagn Invest 2011; 23:241-247.
3. Mandel ID, Wotman S. The salivary secretions in health and disease. Oral Sci Rev 1976; 8:25-47.
4. Robinson L, Bonita EL, Kothapalli S, Craig WR, Fox JD. Use of throat swab or saliva specimens for detection of respiratory viruses in children. Clin Infect Dis 2008; 46:61-64.
5. Feldman M, Plancarte A, Sandoval M, Wilson M, Flisser A. Comparison of two assays (EIA and EITB) and two samples (saliva and serum) for the diagnosis of neurocysticercosis. Trans R Soc Trop Med Hyg 1990; 84:559-562.
6. Jayashree S, Bhan MK, Kumar R, Raj P, Glass R, Bhandari N. Serum and salivary antibodies as indicators of rotavirus infection in neonates. J Infect Dis 1988; 158:1117-1120.
7. Li C, Ha T, Ferguson DA, Chi DS, Zhao R, Patel NR. A newly develop PCR assay of H. pylori in gastric biopsy, saliva, and feces. Evidence of high prevalence of H. pylori in saliva supports oral transmission. Dig Dis Sci 1996; 41:2142-2149.
8. Parry JV, Perry KR, Panday S, Mortimer PP. Diagnosis of hepatitis A and B by testing saliva. J Med Virol 1989; 28:255-260.
9. Perry KR, Brown DW, Parry JV, Panday S, Pipkin C, Richards A. Detection of measles, mumps, and rubella antibodies in saliva using antibody captures radioimmunoassay. J Med Virol 1993; 40:235-240.
10. Schultsz C, Qadri F, Hossain SA, Ahmed F, Ciznar I. Shigella-specific IgA in saliva of children with bacillary dysentery. FEMS Microbiol Immunol 1992; 4:65-72.
11. Holmstrom P, Syrjanen S, Laine P, Valle SL, Suni J. HIV antibodies in whole saliva detected by Elisa and Western blot assays. J Med Virol 1990; 30:245-248.
12. Callahan JD, Brown F, Osorio F et al. Use of a portable real-time reverse transcriptase polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus. J Am Vet Med Assoc 2002; 220:1636-1642.
13. Bender JS, Shen HG, Irwin C, Schwartz KJ, Opriessnig T. Characterization of Erysipelothrix species isolates from clinically affected pigs, environmental samples, and vaccine strains from six recent swine erysipelas outbreaks in the United States. Clin Vaccine Immunol 2010; 17:1605-1611.
14. Prickett J, Simer R, Christopher-Hennings J, Yoon KJ, Evans RB, Zimmerman JJ. Detection of porcine reproductive and respiratory syndrome virus infection in porcine oral fluid samples: a longitudinal study under experimental conditions. J Vet Diagn Invest 2008; 20:156-163.
15. Kittawornrat A, Prickett J, Chittick W et al. Porcine reproductive and respiratory syndrome virus (PRRSv) in serum and oral fluid samples from individual boars: will oral fluid replace serum for PRRSv surveillance? Virus Res 2010; 154:170-176.
16. USDA. Part II: reference of swine health and health management in the United States, 2000, NAHMS swine 2006: National Animal Health Monitoring System, [on line] 2006. Available at (Accessed 30 November 2010); 16-20.
17. Meguro H, Bryant JD, Torrence AE, Wright PF. Canine kidney cell line for isolation of respiratory virus. J Clin Microbiol 1979; 9:175-179.
18. Vincent LA, Larger KM, Ma W et al. Evaluation of hemagglutinin subtype 1 influenza viruses from the United States. Vet Microbiol 2006; 118:212-222.
19. United States Department of Agriculture, National Animal Health Laboratory Network, Protocol BPA-9034.01.
20. Spackman E, Senne DA, Myers TJ et al. Development of a real time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J Clin Microbiol 2002; 40:3256-32560.
21. Pedersen JC. Hemagglutination-inhibition test for avian influenza virus subtype identification and the detection and quantization of serum antibodies to the avian influenza virus. Methods in Molecular Biology, Vol 436. Avian Influenza Virus. Totowa, NJ: Erica Spackman © Humana Press, 2008.
22. Romagosa A, Gramer M, Joo HS, Deen J, Torremorell M. Influenza virus transmission rates in vaccinated and non-vaccinated pig populations. Proceedings of the International Conference on Options for the control of Influenza VII, Hong Kong, China: International Society for Influenza and other Respiratory Diseases, 2010: 9.
23. Heinen PP, van Niewstadt AP, de Boer-Luijtze EA, Bianchi ATJ. Analysis of the quality of protection induced by a porcine influenza A vaccine to challenger with H3N2 virus. Vet Immunol Immunopathol 2001; 82:39-56.
24. Van Reeth K, Labarque G, De Clercq S, Pensaert M. Efficacy of vaccination of pigs with different H1N1 swine influenza viruses using a recent challenge strain and different parameters of protection. Vaccine 2001; 19:4479-4486.
25. Wills RW, Zimmerman JJ, Yoon K-J et al. Porcine reproductive and respiratory syndrome virus: routes of excretion. Vet Microbiol 1997; 57:69-81.
26. Millman ST, Brooks RM, Zimmerman J, Irwin C. Effects of acute influenza virus infection on swine behaviour associated with collection of oral fluid specimens for disease surveillance; in: McGill University, (ed.). Proceedings of the 9th North American Regional Meeting of the International Society for Applied Ethology. Montreal, Canada, 2009; 28.