Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes

Veterinary Microbiology

Volumn 64, Issue 1, 1998, Pages 7-22

  Oleksiewicz, M B ^b   Botner A ^b   Madsen, K G ^a   Storgaard, T ^b  

^a DANISH INSTITUTE OF AGRICULTURAL SCIENCES   (Denmark)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Diagnosis viruses; Pig viruses; PRRSV; RFLP; RT PCR; Vaccinations

Indexed keywords

ARTICLE; CONTROLLED STUDY; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SWINE; VACCINATION; VIRUS INFECTION;

ANIMALS; ANTIBODIES, MONOCLONAL; DENMARK; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; EUROPEAN UNION; FEMALE; MACROPHAGES, ALVEOLAR; MALE; OPEN READING FRAMES; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SEMEN; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SPECIFIC PATHOGEN-FREE ORGANISMS; SWINE; UNITED STATES;

PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS; SUIDAE; SUS SCROFA;

EID: 0031764269     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1135(98)00254-5     Document Type: Article

References (36)

1. Boom R., Sol C.J.A., Salimans M.M.M., Jansen C.L., Wertheim-van Dillen P.M.E., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:1990;495-503.
2. Bøtner A., Nielsen J., Bille-Hansen V. Isolation of porcine reproductive and respiratory syndrome (PRRS) virus in a Danish swine herd and experimental infection of pregnant gilts with the virus. Vet. Microbiol. 40:1994;351-360.
3. Bøtner A., Strandbygaard B., Sørensen K.J., Have P., Madsen K.G., Smedegaard Madsen E., Alexandersen S. Appearance of acute PRRS-like symptoms in sow herds after vaccination with a modified live PRRS vaccine. Vet. Rec. 141:1997;497-499.
4. Christopher-Hennings, J., Nelson, E.A., Hines, R.J., Nelson, J.K., Swenson, S.L., Zimmerman, J.J., Chase, C.L., Yaeger, M.J., Benfield, D.A., 1995a. Persistence of porcine reproductive and respiratory syndrome virus in serum and semen of abult boars. J. Vet. Diag. Invest. 7, 456-464.
5. Christopher-Hennings, J., Nelson, E.A., Nelson, J.K. Hines, R.J., Swenson, S.L., Hill, H.T., Zimmerman, J.J., Katz, J.B., Yaeger, M.J., Chase, C.C., 1995b. Detection of porcine reproductive and respiratory syndrome virus in boar semen by PCR. J. Clin. Microbiol. 33, 1730-1734.
6. Duan X., Nauwynck H.J., Pensaert M.B. Virus quantification and identification of cellular targets in the lungs and lymphoid tissues of pigs at different time intervals after inoculation with porcine reproductive and respiratory syndrome virus (PRRSV). Vet. Microbiol. 56:1997;9-19.
7. Gilbert S.A., Larochelle R., Magar R., Cho H.J., Deregt D. Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay. J. Clin. Microbiol. 35:1997;264-267.
8. Gradil C., Dubuc C., Eaglesome M.D. Porcine reproductive and respiratory syndrome virus: Seminal transmission. Vet. Rec. 138:1996;521-522.
9. Kapur V., Elam M.R., Pawlovich T.M., Murtaugh M.P. Genetic variation in porcine reproductive and respiratory syndrome virus isolates in the mid-western United States. J. Gen. Virol. 77:1996;1271-1276.
10. Kim H.S., Kwang J., Yoon I.J., Joo H.S., Frey M.L. Enhanced replication of poroine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line. Arch. Virol. 133:1993;477-483.
11. Kono Y., Kanno T., Shimizu M., Yamada S., Ohashi S., Nakamine M., Shirai J. Nested PCR for detection and typing of porcine reproductive and respiratory syndrome (PRRS) virus in pigs. JV Medical Science. 58:1996;941-946.
12. Lai M.C. RNA recombination in animal and plant viruses. Microbiol. Rev. 56:1992;61-79.
13. Lai M.C., Banner L.R. Random nature of coronavirus RNA recombination in the absence of selection pressure. Virology. 185:1991;441-445.
14. Larochelle R., Magar R. Evaluation of the presence of porcine reproductive and respiratory syndrome virus in packaged pig meat using virus isolation and polymerase chain reaction (PCR) method. Vet. Microbiol. 58:1997;1-8.
15. Legeay O., Bounaix S., Denis M., Arnauld C., Hutet E., Cariolet R., Albina E., Jestin A. Development of a RT-PCR test coupled with a microplate colorimetric assay for the detection of a swine Arterivirus (PRRSV) in boar semen. J. Virol. Methods. 68:1997;65-80.
16. Madsen K.G., Hansen C.M., Madsen E.S., Strandbygaard B., Bøtner A., Sørensen K.J. Detection of porcine reproductive and respiratory syndrome virus of the American-type in Danish swine herds. Arch. Virol. 143:1998;1683-1700.
17. Mardassi H., Wilson L., Mounir S., Dea S. Detection of porcine reproductive and respiratory syndrome virus and efficient differentiation between Canadian and European strains by reverse transcription and PCR amplification. J. Clin. Microbiol. 32:1994;2197-2203.
18. Meng X.J., Paul P.S., Halbur P.G., Lum M.A. Phylogenetic analyses of the putative M (ORF 6) and N (ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): Implication for the existence of two genotypes of PRRSV in the USA and Europe. Arch. Virol. 140:1995;745-755.
19. Meulenberg J.J.M., Hulst M.M., De Meijer E.J., Moonen P.L.J.M., Den Besten A., De Kluyver E.P., Wensvoort G., Moormann R.J.M. Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology. 192:1993;62-72.
20. Meulenberg J.M., Petersen-den B.A., Kluyver E.d., Moormann R.M., Schaaper W.M., Wensvoort G., De K.E. Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus. Virology. 206:1995;155-163.
21. Meulenberg J.J., Besten A.d., Kluyver E.d., Nieuwstadt A.v., Wensvoort G., Moormann R.J. Molecular characterization of Lelystad virus. Vet. Microbiol. 55:1997;197-202.
22. Murphy N.R., Leinbach S.S., Hellwig R.J. A potent, cost-effective RNase inhibitor. BioTechniques. 18:1995;1068-1073.
23. Murtaugh M.P., Elam M.R., Kakach L.T. Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus. Arch. Virol. 140:1995;1451-1460.
24. Nielsen, J., Bøtner, A., Oleksiewicz, M.B., Storgaard, T., 1998. Experimental inoculation of late-term pregnant sows with a field isolate of PRRS vaccine-like virus, In: Proc. 15th Int. Pig Vet. Soc. Congr., Birmingham, UK.
25. Nielsen T.L., Nielsen J., Have P., Baekbo P., Hoff J.R., Botner A. Examination of virus shedding in semen from vaccinated and from previously infected boars after experimental challenge with porcine reproductive and respiratory syndrome virus. Vet. Microbiol. 54:1997;101-112.
26. Sellner L.N., Coelen R.J., Mackenzie J.S. Revers transcriptase inhibits Taq polymerase activity. Nucleic Acids Res. 20:1992;1487-1490.
27. Shin J., Torrison J., Choi C.S., Gonzalez S.M., Crabo B., Molitor T.W. Monitoring of porcine reproductive and respiratory syndrome virus infection in boars. Vet. Microbiol. 55:1997;337-346.
28. Storgaard, T., 1998. (unpub).
29. Suarez P., Zardoya R., Martin M.J., Prieto C., Dopazo J., Solana A., Castro J.M. Phylogenetic relationships of european strains of porcine reproductive and respiratory syndrome virus (PRRSV) inferred from DNA sequences of putative ORF-5 and ORF-7 genes. Virus Res. 42:1996;159-165.
30. Suárez P., Zardoya R., Prieto C., Solana A., Tabarés E., Bautista J.M., Castro J.M. Direct detection of the porcine reproductive and respiratory syndrome (PRRS) virus by reverse polymerase chain reaction (RT-PCR). Arch. Virol. 135:1994;89-99.
31. Sur J.-H., Doster A.R., Christian J.S., Galeota J.A., Wills R.W., Zimmerman J.J., Osorio F.A. Porcine reproductive and respiratory syndrome virus replicates in testicular germ cells, alters spermatogenesis, and induces germ cell death by apoptosis. J. Virol. 71:1997;9170-9179.
32. Swenson S.L., Hill H.T., Zimmerman J.J., Evans L.E., Landgraf J.G., Wills R.W., Sanderson T.P., McGinley M.J., Brevik A.K., Ciszewski D.K. Excretion of porcine reproductive and respiratory syndrome virus in semen after experimentally induced infection in boars. J. Am. Vet. Med. Assoc. 204:1994;1943-1948.
33. Sørensen K.J., Bøtner A., Smedegaard Madsen E., Strandbygaard B., Nielsen J. Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus. Vet. Microbiol. 56:1997;1-8.
34. Sørensen K.J., Strandbygaard B., Bøtner A., Madsen E.S., Nielsen J., Have P. Blocking ELISA's for the distinction between antibodies against European and American strains of porcine reproductive and respiratory syndrome (PRRS) virus. Vet. Microbiol. 60:1998;169-177.
35. Van Woensel P., Van der Wouw J., Visser N. Detection of porcine reproductive respiratory syndrome virus by the polymerase chain reaction. J. Virol. Methods. 47:1994;273-278.
36. Yoon K.J., Zimmerman J.J., McGinley M.J., Landgraf J., Frey M.L., Hill H.T., Platt K.B. Failure to consider the antigenic diversity of porcine reproductive and respiratory syndrome (PRRS) virus isolates may lead to misdiagnosis. J. Vet. Diagn. Invest. 7:1995;386-387.